QCM-D Study on Relationship of PEG Coated Stainless Steel Surfaces to Protein Resistance

Authors: Norzita Ngadi, John Abrahamson, Conan Fee, Ken Morison

Abstract:

Nonspecific protein adsorption generally occurs on any solid surfaces and usually has adverse consequences. Adsorption of proteins onto a solid surface is believed to be the initial and controlling step in biofouling. Surfaces modified with end-tethered poly(ethylene glycol) (PEG) have been shown to be protein-resistant to some degree. In this study, the adsorption of β-casein and lysozyme was performed on 6 different types of surfaces where PEG was tethered onto stainless steel by polyethylene imine (PEI) through either OH or NHS end groups. Protein adsorption was also performed on the bare stainless steel surface as a control. The adsorption was conducted at 23 °C and pH 7.2. In situ QCM-D was used to determine PEG adsorption kinetics, plateau PEG chain densities, protein adsorption kinetics and plateau protein adsorbed quantities. PEG grafting density was the highest for a NHS coupled chain, around 0.5 chains / nm2. Interestingly, lysozyme which has smaller size than β-casein, appeared to adsorb much less mass than that of β- casein. Overall, the surface with high PEG grafting density exhibited a good protein rejection.

Keywords: QCM-D, PEG, stainless steel, β-casein, lysozyme.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1080088

References:

[1] P. Kingshott, W. Jiang, G. B. Nikolaj and G. Lone, G, "Covalent attachment of Poly(ethylene glycol) to surfaces, critical for reducing bacterial adhesion", Langmuir. vol. 19, pp. 6912-6921, 2003
[2] L.D.Unsworth, H.Sheardown and J.L.Brash, "Protein resistance of surfaces prepared by sorption of end-thiolated poly(ethylene glycol) to gold:Effect of surface chain density", Langmuir. vol. 21. pp.1036-1041, 2005
[3] T.Satomi, Y.Nagasaki, H.Kobayashi, T.Tateishi, K.Kataoka and H.Otsuka, "Physicochemical characterization of densely packed poly (ethylene glycol) layer for minimizing nonspecific protein adsorption", Journal of Nanoscience and Nanotechnology, vol. 7, pp. 2394-2399, 2007
[4] J. Wei, D.B. Ravn, L. Gram and P. Kingshott, "Stainless Steel Modified with PEG can Prevent Protein Adsorption but not Bacterial Adhesion", Colloids and Surfaces B: Biointerfaces, vol. 32, pp. 275-291.2005
[5] J.G. Archambault and J.L. Brash, "Protein Repellent Polyurethane-Urea Surfaces by Chemical Grafting of Hydroxyl-Terminated Poly(ethylene oxide): Effects of Protein Size and Charge", Colloids and Surfaces B: Biointerfaces, vol. 33, pp. 111-120, 2004
[6] A. Roosjen, H.C. Mei, H.J. Busscher and W. Norde, "Microbial Adhesion to Poly(ethylene oxide) Brushes: Influence of Polymer Chain Length and Temperature", Langmuir, vol. 20, pp. 10949-10955, 2004
[7] W.Norde and D.Gage, "Interaction of BSA and human blood plasma with PEO-tethered surfaces: influence of PEO chain length, grafting density and temperature", Langmuir, vol. 20, pp. 4162-4167, 2004
[8] K.L.Prime and G.M.Whitesides, "Adsorption of Proteins onto Surfaces Containing End-Attached Oligo( ethylene oxide): A Model System Using Self-Assembled Monolayers", Journal American Chemistry Society, vol. 115, pp. 10714-10721, 1993
[9] K.Uchida, H.Otsuka, M.Kaneko, K.Kataoka and Y.Nagasaki, "A reactive poly (ethylene glycol) layer to achieve specific surface plasmon resonance sensing with a high S/N: The substantial role of a short underbrushed PEG layer in minimizing nonspecific adsorption", Analytical Chemistry, vol. 77, pp. 1075-1080
[10] C.Yoshikawa, A.Goto. Y.Tsuji, T.Fukuda, T.Kimura, K.Yamamoto and A.Kishida, "Protein repellency of well-defined, concentrated poly (2- hydroxyethyl methacrylate) brushes by the size exclusion effect", Macromolecules, vol. 39, pp. 2284-2290, 2006
[11] Y.J.Wu, R.B.Timmons, J.S.Jen and F.E.Molock, "Non-fouling surfaces produced by gas phase pulsed plasma polymerization of an ultra low molecular weight ethylene oxide containing monomer", Colloids and surfaces B: Biointerfaces, vol. 18, pp. 235-248, 2000
[12] N.Weber, H.Peter, and J.Kohn, "Formation of viscoelastic protein layers on polymeric surfaces relevant to platelet adhesion", Wiley InterScience, pp. 420-427,2005
[13] F. Hook, J. Voros, M. Rodahl, R. Kurrat, P. Boni, J.J. Ramsden, M. Textor, N.D. Spencer, P. Tengvall, J. Gold and B. Kasemo, "A Comparative Study of Protein Adsorption on Titanium Oxide Surfaces Using in situ Ellipsometry, Optical Waveguide Lightmode Spectroscopy and Quartz Crystal Microbalance/Dissipation". Colloids and Surfaces B: Biointerfaces, pp. 155-170, 2002.
[14] M.T. Muller, X. Yan, S. Lee, S.S. Perry and N.D. Spencer, N.D. (2005). "Lubrication Properties of a Brushlike Copolymer as a Function of the Amount of Solvent Absorbed within the Brush". Macromolecules, pp. 5706-5713, 2005.
[15] I.M. Nnebe, R.D. Tilton, J.W. Schneider, "Direct Force Measurement of the Stability of Poly(ethylene glycol)-Polyethylenimine Graft Films", Journal of Colloid and Interface Science, vol. 276, pp. 306-316, 2004.
[16] R.Fukai. P.H.R.Dakwa and W.Chen, "Strategies toward biocompatible artificial implants:grafting of functionalized poly(ethylene glycol)s to poly(ethylene terephthalate) surfaces", Journal of Polymer Science:Part A:Polymer Chemistry. vol. 42, pp. 5389-5400, 2004.
[17] R. Michel, S. Pasche, M. Textor and D. G. Castner, "Influence of PEG Architecture on Protein Adsorption and Conformation", Langmuir, vol. 21, pp. 12327-12332, 2005.
[18] A.Helparin, "Polymer brushes that resist adsorption of model proteins:Design parameters", Langmuir, vol. 15, pp. 2525-2533, 1999
[19] N.Nath, J.Hyun, H.Ma and A.Chilkoti, "Surface Engineering for Control of Protein and Cell Interactions", Surface Science, vol. 570, pp. 98-110, 2004.
[20] M.Malmsten, K.Emoto and J.M.V.Alstine, "Effect of chain density on inhibition of protein adsorption by Poly(ethylene glycol) based coatings", Journal of Colloidal and Interface Science, vol. 202, pp. 507- 517, 1998.