Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30172
Effects of Functional Protein on Osteoblasts in Rat

Authors: Jie Sun, Guoyou Yin, Xianqing Zhang, Qiusheng She, Zhaohui Xie, Lanying Chen, Anfang Zhao

Abstract:

To assess the effects of functional protein on osteoblast, Large quantity of high-purity osteoblasts had been cultivated successfully by adopting sequential enzyme digestion. The growth curve of osteoblasts was protracted by cell counting. Proliferation of osteoblasts was assessed by MTT colorimetry. The experimental results show the functional protein can enhance proliferation, the properties of adhesion and discuss the effect of osteopontin on osteoblast.

Keywords: functional protein, osteoblast, MTT

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1078164

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 892

References:


[1] Senger DR, Wirth DF, Hynes RO (1979) Cell .16:885-893
[2] Liaw L, Skinner MP, Raines EW et al (1994) J. Clin. Inves. 95,713-724
[3] Smith LL, Cheung HK, Ling LE et al (1996) J. Biol. Chem. 271,28485-28491
[4] Yokosaki Y, Matsuura N, Sasaki T et al (1999) J. Biol. Chem. 274,36328-36334
[5] S├©rensen ES, H├©rup P Petersen TE (1993) Protein Science .4,2040-2049
[6] Giachelli CM and Steitz S (2000) Matrix Biology. 19,615-622
[7] Denhardt DT, Noda M, O-Regan AW et al (2001) J. Clin. Invest. 107,1055-1061
[8] Khan SA, Lopez-Chua CA, Zhang J et al (2002) J.Cell. Biochem. 85,728-736
[9] Senger DR, Perruzzi CA, Papadopoulos A et al (1989) Biochimica et Biophysica Acta. 996,43-48
[10] Rittling SR and Novck KE (1997) Cell Growth and Differentiation.8,1061-1069
[11] Azuma N and Yamauchi K (1987) J.Dairy Res.54, 199-205
[12] Noda M, Tsuji K, Nifuji A (2003) (J). Clin Calcium.13,464-466
[13] Wan YM, Ma YJ, Zhang XY(2005) (J). Acta Physiologica Sinica. 57,384-388
[14] Jie Sun, Guoyou Yin, Ning Liu.(2010).Milk Science International.65,131-134.
[15] Cuq P, Marcelli C, Cousse H (1995) C R Seances Soc Biol Fil.189,303-12
[16] Buckley MJ, Banes AJ, Jordan RD (1990) J Oral Maxillofac Surg.48,276-282; discussion 282-283
[17] Lucy L, Michael PS, Elaine WR (1995) J. Clin. Invest .2,713-724
[18] Jun-Hyeog Jang and Ji-Hyun Kim (2005) Bioechnology Leters.27,1767-1770
[19] Kayla JB, Gerald AM, Martin SJ (1998) J. Cell Sci. 111,1165-1174
[20] Liaw L, Almeida M, Hart CE (1994) (J). Circ Res .74,214-224
[21] Liu CJ, Liu JG, Yu TC et al (2007) Chinese Journal of Gerontology. 7,525-527.
[22] Liaw L, Almeida M, Hart CE (1994) (J). Circ Res 74,214-224
[23] S├©rensen S, Justesen SJ, Johnsen AH (2003) Protein Expression and Purification 30,238-245
[24] Kon S, Maeda M, Segawa T et al (2000) J. Cell. Biochem.77,487-498.
[25] Liu CJ, Liu JG, Yu TC et al (2007)Chinese Journal of Gerontology 7,525-527.