Cultivation of Thymus by In Vitro And Hydroponics Combined Method
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Cultivation of Thymus by In Vitro And Hydroponics Combined Method

Authors: E. Sargsyan, A. Vardanyan, L. Ghalachyan, S. Bulgadaryan


Our results showed that for the growth of qualitative seedling and vegetative raw material of ðó. marschallianus Willd. and T. serphyllum L. it is more profitable to use the in vitro and hydroponics combined method. In in vitro culture it is possible to do micro-propagation whole year with 98-99% rhizogenesis. 30000 micro-plants were obtained from one explant during 9 months. Hydroponic conditions provide the necessary microclimate for microplants where the survival rate without acclimatization was 93.3%. The essential oil content in hydroponic dry herb of both species in vegetative and blossom phase was 1.3% whereas in wild plants it was 1.2%, the content of extractive substances and vitamin C also exceeded wild plants. Our biochemical and radiochemical investigations indicated that the medicinal raw materials obtained from hydroponic and wild plants of Thymus species correspond to the demands of SPh XI, and the content of artificial radionuclides does not exceed the MACL.

Keywords: Hydroponics, In vitro, Micro-propagation, Thymus

Digital Object Identifier (DOI):

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[1] R.G.Butenko Biology of Cultivated Cells of Streptophyta by In vitro and Biotechnology on its Base, Monograph, Moscow, p. 159, 1999 (in Russian)
[2] G.S. Davtyan Hydroponic Production Achievement for Agrochemical Science. Yerevan, pp.352-365, 1980 (in Russian).
[3] Essential Oils Market Information Booklet, IENICA, May 2004,
[4] European Pharmacopoeia 2005. 5th edition, Strasburg-, Version 5.1 EDQM
[5] V.P.Georgievskiy, N.F. Komissarenko, S.E. Dmitruk The Biologically Active Substances of Herbal Plants, 1990, p. 191-242 (in Russian)
[6] N.V.Kataeva, R.G.Butenko, Clonal Micro-propagation of Plants. Moscow, p.96, 1983 (in Russian)
[7] T. Murashige, F.Skoog A Revised Medium for Rapid Growing and Bioassay with Tobacco Tissue Culture. // Physical Plantarium, V.1 (15), pp.473-497, 1962.
[8] N.I.Grinkevich, L.N. Safronich The Chemical Analysis of Herbal Plants. p. 175, 1983 (in Russian)
[9] F.I.Pavlotskaya Methods of 90 Sr and other long-living isotopes determination. In collection. Physiological-chemical methods of soil study. p. 126, 1966 (in Russian)
[10] B.C. Prister, C.C. Zubach The using of Arsenazo III defining U in the soils and biological objects. "Radiochemistry" 10, Issue 6, pp. 743-748, 1968 (in Russian)
[11] Red Book of Republic of Armenia. Second edition, p. 7, 2010.
[12] E.D.Sargsyan, N.N. Tambian, S.Kh. Mairapetyan Cultivation of Lawsonia by Hydroponics and In Vitro Combined Method in Armenia.- Transaction of International Forum on Problems of Science, Technology & Education, Moscow, pp. 45-47, 1998 (in Russian)
[13] A.O.Sari, B.Oguz Importance of medicinal and aromatic plants in horticultural crops of Eagean and Marmara Regions. Eagean Agricultural Research Institute Publications, Izmir, 2000, N 98, p. 82
[14] State Pharmacopoeia USSR, 11th edition, Moscow: "Medicine--, pp. 323-325, 1990 (in Russian)
[15] The Radiation Safety Norms. Hygienic Norms, Moscow, sanitary rules and regulations centre, Ministry of public health of Russia, p. 116, 1999 (in Russian)
[16] A.I. Yermakov, B.B. Arasimovich, M.N. Smirnova-Ikonkinova, I.K. Murri. Methods of plants biochemical investigations. Moscow, pp. 90- 60, 1952 (in Russian)