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Sophorolipids Production by Candida Bombicola using Synthetic Dairy Wastewater
Abstract:Sophorolipids (SLs) production by the yeast Candida bombicola was studied in batch shake flasks using synthetic dairy wastewaters (SDWW) with or without any added external carbon and nitrogen sources. A maximum SLs production of 38.76 g/l was observed with the SDWW supplemented with low cost substrate of sugarcane molasses at 50 g/l and soybean oil at 50 g/l. When the SDWW was supplemented with more costly glucose, yeast extract, urea and soybean oil, the production, however, got lowered to only 29.49 g/l, but with a maximum biomass production of 17.38 g/l together with a complete utilization of the carbon sources.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1077679Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2701
 R. K. Hommel, and K. Huse, "Regulation of sophorose lipid production by Candida (Torulopsis) apicola," Biotechnol. Lett., 1993, vol 15, pp. 853-858.
 2. H. J. Asmer, S. Lang, F. Wagner, and V. Wray, "Microbial production, structure elucidation and bioconversion of sophorose lipids," JAOCS, 1988, vol. 65, pp. 1460-1466.
 A. P. J. Gorin, J. F. T. Spencer, and A. P. Tulloch, "Hydroxy fatty acid glycosides of sophorose from Torulopsis magnoliae," Can. J. Chem., 1961, vol. 39, pp. 846-895.
 4. D. K. Y. Solaiman, R. D. Ashby, J. A. Zerkowski, and T. A. Foglia, "Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola," Biotechnol. Lett., 2007, vol. 29, pp. 1341-1347.
 U. Rau, S. Hammen, R. Heckmann, V. Wray, and S. Lang, "Sophorolipids: a source for novel compounds," Ind. Crop Prod. 2001, vol. 13, pp. 85-92.
 C. N. Mulligan, R. N. Yong, and B. F. Gibbs, "Heavy metal removal from sediments by biosurfactants," J. Hazard. Mater. 2001, vol 85, pp. 111-125.
 7. C. Schippers, K. Gessner, T. Muller, and T. Scheper, "Microbial degradation of phenanthrene by addition of a sophorolipid mixture," J. Biotechnol. 2000, vol 83, pp. 189-198.
 L. R. Lynd, C. E. Wyman, and T. U. Gerngross, "Biocommodity engineering," Biotechnol. Prog. 1999, vol 15, pp. 777-793.
 A. Daverey, and K. Pakshirajan, Production, characterization, and properties of sophorolipids from the yeast Candida bombicola using a low-cost fermentative medium. Appl. Biochem. Biotechnol., to be published.
 M. Deshpande, L. Daniels, "Evaluation of sophorolipid biosurfactant production by Candida bombicola using animal fat," Bioresour. Technol. 1995, vol. 54, pp. 143-150.
 B. D. Tripathi, and A. R. Upadhyay, "Dairy effluent polishing by aquatic macrophytes," Water Air Soil Pollut., 2003, vol. 143, pp. 377- 385.
 R. Willey, "Fats, oils and greases: The minimization and treatment of wastewaters generated from oil refining and margarine production," Ecotoxicol. Environ. Saf., 2001, vol. 50, pp. 127-133.
 M. C. Cammarota, and D. M. G. Freire, "A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content," Bioresour. Technol., 2006, vol. 97, pp. 2195-2210.
 K. Tano-Debrah, S. Fukuyama, N. Otonari, F. Taniguchi, and M. Ogura, "An inoculum for the aerobic treatment of wastewaters with high concentrations of fats and oils," Bioresour. Technol., 1999, vol. 69, pp. 133-139.
 L. Masse, K. J. Kennedy, and S. Chou, "Testing of alkaline and enzymatic hydrolysis pretreatments for oil particles in slaughterhouse wastewater" Bioresour. Technol., 2001, vol. 77, pp. 145-155.
 G. Sivalingam, S. Chattopadhyay, and G. Madras, "Solvent effects on lipase catalyzed biodegradation of poly (epsilon-caprolactone) in solution," Polym. Degrad. Stab., 2003, vol. 79, pp. 413-418.
 M. C. M. R. Leal, D. M. G. Freire, M. C. Cammarota, and G. L. Sant-Anna Jr., "Effect of enzymatic hydrolysis on anaerobic treatment of dairy wastewater," Process Biochem. 2006, vol. 41, pp. 1173-1178.
 S. Joshi, C. Bharucha, S. Jha, S. Yadav, A. Nerurkar, and A. J. Desai, "Biosurfactant production using molasses and whey under thermophilic conditions," Bioresour. Technol., 2008, vol. 99, pp. 195-199.
 T. A. Scott, Jr., and E. H. Melvin, "Determination of dextran with anthrone," Anal. Chem., 1953, vol. 25, pp. 1656-1661.
 Y. H. Hu, and L-K. Ju, "Sophorolipid production from different lipid precursors observed with LC-MS," Enzyme Microb. Technol., 2001, vol. 29, pp. 593-601.
 21. A. Daverey, and K. Pakshirajan, Production of sophorolipids by the yeast Candida bombicola using simple and low cost fermentative media," Food Res. Intern., to be published.