Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30174
Sophorolipids Production by Candida Bombicola using Synthetic Dairy Wastewater

Authors: A. Daverey, K. Pakshirajan, P. Sangeetha

Abstract:

Sophorolipids (SLs) production by the yeast Candida bombicola was studied in batch shake flasks using synthetic dairy wastewaters (SDWW) with or without any added external carbon and nitrogen sources. A maximum SLs production of 38.76 g/l was observed with the SDWW supplemented with low cost substrate of sugarcane molasses at 50 g/l and soybean oil at 50 g/l. When the SDWW was supplemented with more costly glucose, yeast extract, urea and soybean oil, the production, however, got lowered to only 29.49 g/l, but with a maximum biomass production of 17.38 g/l together with a complete utilization of the carbon sources.

Keywords: Candida bombicola, dairy wastewater, fat and oil, sophorolipids.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1077679

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2701

References:


[1] R. K. Hommel, and K. Huse, "Regulation of sophorose lipid production by Candida (Torulopsis) apicola," Biotechnol. Lett., 1993, vol 15, pp. 853-858.
[2] 2. H. J. Asmer, S. Lang, F. Wagner, and V. Wray, "Microbial production, structure elucidation and bioconversion of sophorose lipids," JAOCS, 1988, vol. 65, pp. 1460-1466.
[3] A. P. J. Gorin, J. F. T. Spencer, and A. P. Tulloch, "Hydroxy fatty acid glycosides of sophorose from Torulopsis magnoliae," Can. J. Chem., 1961, vol. 39, pp. 846-895.
[4] 4. D. K. Y. Solaiman, R. D. Ashby, J. A. Zerkowski, and T. A. Foglia, "Simplified soy molasses-based medium for reduced-cost production of sophorolipids by Candida bombicola," Biotechnol. Lett., 2007, vol. 29, pp. 1341-1347.
[5] U. Rau, S. Hammen, R. Heckmann, V. Wray, and S. Lang, "Sophorolipids: a source for novel compounds," Ind. Crop Prod. 2001, vol. 13, pp. 85-92.
[6] C. N. Mulligan, R. N. Yong, and B. F. Gibbs, "Heavy metal removal from sediments by biosurfactants," J. Hazard. Mater. 2001, vol 85, pp. 111-125.
[7] 7. C. Schippers, K. Gessner, T. Muller, and T. Scheper, "Microbial degradation of phenanthrene by addition of a sophorolipid mixture," J. Biotechnol. 2000, vol 83, pp. 189-198.
[8] L. R. Lynd, C. E. Wyman, and T. U. Gerngross, "Biocommodity engineering," Biotechnol. Prog. 1999, vol 15, pp. 777-793.
[9] A. Daverey, and K. Pakshirajan, Production, characterization, and properties of sophorolipids from the yeast Candida bombicola using a low-cost fermentative medium. Appl. Biochem. Biotechnol., to be published.
[10] M. Deshpande, L. Daniels, "Evaluation of sophorolipid biosurfactant production by Candida bombicola using animal fat," Bioresour. Technol. 1995, vol. 54, pp. 143-150.
[11] B. D. Tripathi, and A. R. Upadhyay, "Dairy effluent polishing by aquatic macrophytes," Water Air Soil Pollut., 2003, vol. 143, pp. 377- 385.
[12] R. Willey, "Fats, oils and greases: The minimization and treatment of wastewaters generated from oil refining and margarine production," Ecotoxicol. Environ. Saf., 2001, vol. 50, pp. 127-133.
[13] M. C. Cammarota, and D. M. G. Freire, "A review on hydrolytic enzymes in the treatment of wastewater with high oil and grease content," Bioresour. Technol., 2006, vol. 97, pp. 2195-2210.
[14] K. Tano-Debrah, S. Fukuyama, N. Otonari, F. Taniguchi, and M. Ogura, "An inoculum for the aerobic treatment of wastewaters with high concentrations of fats and oils," Bioresour. Technol., 1999, vol. 69, pp. 133-139.
[15] L. Masse, K. J. Kennedy, and S. Chou, "Testing of alkaline and enzymatic hydrolysis pretreatments for oil particles in slaughterhouse wastewater" Bioresour. Technol., 2001, vol. 77, pp. 145-155.
[16] G. Sivalingam, S. Chattopadhyay, and G. Madras, "Solvent effects on lipase catalyzed biodegradation of poly (epsilon-caprolactone) in solution," Polym. Degrad. Stab., 2003, vol. 79, pp. 413-418.
[17] M. C. M. R. Leal, D. M. G. Freire, M. C. Cammarota, and G. L. Sant-Anna Jr., "Effect of enzymatic hydrolysis on anaerobic treatment of dairy wastewater," Process Biochem. 2006, vol. 41, pp. 1173-1178.
[18] S. Joshi, C. Bharucha, S. Jha, S. Yadav, A. Nerurkar, and A. J. Desai, "Biosurfactant production using molasses and whey under thermophilic conditions," Bioresour. Technol., 2008, vol. 99, pp. 195-199.
[19] T. A. Scott, Jr., and E. H. Melvin, "Determination of dextran with anthrone," Anal. Chem., 1953, vol. 25, pp. 1656-1661.
[20] Y. H. Hu, and L-K. Ju, "Sophorolipid production from different lipid precursors observed with LC-MS," Enzyme Microb. Technol., 2001, vol. 29, pp. 593-601.
[21] 21. A. Daverey, and K. Pakshirajan, Production of sophorolipids by the yeast Candida bombicola using simple and low cost fermentative media," Food Res. Intern., to be published.