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Comparative Study on the Antioxidant Activity of Leaf Extract and Carotenoids Extract from Ipomoea batatas var. Oren (Sweetpotato) Leaves

Authors: Seow-Mun Hue, Amru Nasrulhaq Boyce, Chandran Somasundram


Ipomoea batatas (Sweetpotato) is currently ranked sixth in the total world food production and are planted mainly for their storage roots. The present study was undertaken to evaluate and compare the antioxidant properties of the leaf and carotenoids extract from the Ipomoea batatas var. Oren leaves. Total flavonoids in the leaf extract was 144.6 ± 40.5 μg/g compared to 114.86 ± 4.35 μg/g catechin equivalent in the carotenoids extract. Total polyphenols in the leaf extracts (3.470 ± 0.024 GAE g/100g DW) was slightly higher compared to carotenoids extract (2.994 ± 0.078 GAE g/100g DW). The carotenoids extract marked a higher radical scavenging capacity with the IC50= 491.86 μg/ml compared to leaf extract (IC50= 545.39 μg/ml). Concentration-dependent reducing activity was observed for both extracts. Thus, the carotenoids extraction process retained most of the antioxidant capacity from the leaves and can be made into potential natural yellow dye with antioxidant property.

Keywords: antioxidants, carotenoids extract, Ipomoea batatas, sweetpotato leaves

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[1] A. Bolivar, C. Cevallos and C. Z. Luis, "Stoichiometric and kinetic studies of phenolic antioxidants from Andean purple corn and redfleshed sweetpotato," Journal of Agricultural Food Chemistry, vol. 51, pp. 3313-3319, 2003.
[2] S. Furuta, I. Suda, Y. Nishiba and O. Yamakawa, "High tertbutylperoxyl radical scavenging activities of sweetpotato cultivars with purple flesh," Food Sci. Technol. Int. Tokyo, vol. 29, pp. 33-35, 1998.
[3] K. Ishiguro and M. Yoshimoto, "Lutein Content of Sweetpotato leaves II. Cultivar Differences, Distribution in Leaves at Different Positions and Changes during storage," National Agricultural Research Centre for Kyushu Okinawa region (KONARC), Sweetpotato Research Front, vol. 20, pp. 4, 2007.
[4] S. M. Hue, A. N. Boyce and S. Chandran, "Influence of growth stage and variety on the pigment levels in Ipomoea batatas (Sweetpotato) leaves," African Journal of Agricultural Research, vol. 6(10), pp. 2379- 2385, 2011.
[5] S. M. Islam, M. Yoshimoto, S. Yahara, S. Okuno, K. Ishiguro and O. Yamakawa, "Identification and Characterization of Foliar Polyphenolic Composition in Sweetpotato (Ipomoea batatas L.) Genotypes," Journal of Agricultural Food Chemistry, vol. 50, pp. 3718-3722, 2002.
[6] K. M. Fisch, V. Bohm, A. D. Wright and G. M. Konig, "Antioxidative meroterpenoids from the brown alga Cystoseira crinite," Journal of Natural Products, vol. 66, pp. 968-975, 2003.
[7] M. Y. Shon, T. H. Kim and N. J. Sung, "Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts," Food Chemistry, vol. 82, pp. 593-597, 2003.
[8] M. Wettasinghe and F. Shahidi, "Antioxidant and free radicalscavenging properties of ethanolic extracts of defatted borage (Borago officinallis L.) seeds," Food Chemistry, vol. 67, pp. 399-414, 1999.
[9] N. Salah, N. J. Miller, G. Paganga, L. Tijburg, G. P. Bolwell and C. A. Rice-Evans, "Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants," Archives of Biochemistry and Biophysics, vol. 322, pp. 339−346, 1995.
[10] R. Edge, D. J. McGarvey and T. G. Truscott, "The carotenoids as antioxidants- a review," Journal of Photochemistry and Photobiology, vol. 41, pp. 189-200, 1997.
[11] A. M. Papas, "Beyond ╬▒-tocopherol: the role of the other tocopherols and tocotrienols," in Phytochemicals in Nutrition and Health, M. S. Meskin, W. R. Bidlack, A. J. Davies, & S. T. Omaye, New York: CRC press, 2002, pp. 61-78.
[12] M. Kimura and D. B. Rodriguez-Amaya, "A scheme for obtaining standards and HPLC quantification of leafy vegetable carotenoids," Journal of Food Chemistry, vol. 78, pp. 389-398, 2002.
[13] A. L. Waterhouse, "Determination of Total Phenolics," Current Protocols in Food Analytical Chemistry, pp. I1.1.1- I1.1.8, 2002.
[14] M. Oyaizu, "Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine," Japanese Journal of Nutrition, vol. 103, pp. 413-419, 1986.
[15] S. H. Bae and H. J. Suh, "Antioxidant activities of five different mulberry cultivars in Korea," LWT-Food Science and Technology, vol. 40, pp. 955-962, 2007.
[16] B. Sun, J.M. Ricardo-da-Silva and I. Spranger, "Critical factors of vanillin assay for catechins and proanthocyanidins," Journal of Agricultural Food Chemistry, vol. 46, pp. 4267-4274, 1998.
[17] M. M. E. Mervat and A. A. T. Hanan, "Antioxidant Activities, Total Anthocyanins, Phenolics and Flavonoids Contents of Some Sweetpotato Genotypes under Stress of Different Concentrations of Sucrose and Sorbitol," Australian Journal of Basic and Applied Sciences, vol. 3(4), pp. 3609-3616, 2009.
[18] N. C. Cook and S. Samman, "Flavonoids- Chemistry, metabolism, Cardioprotective Effects, and dietary sources," Nutritional Biochemistry, vol. 7, pp. 66-76, 1996.
[19] I. E. Dreosti, "Antioxidant polyphenols in tea, cocoa and wine," Nutrition, vol. 16, pp. 692-694, 2000.
[20] D. Marinova, F. Ribarova and M. Atanassova, "Total phenolics and total flavonoids in Bulgarian fruits and vegetables," Journal of the University of Chemical Technology and Metallurgy, vol. 40(3), pp. 255-260, 2005.
[21] Y. Nakamura, S. Watanabe, N. Miyake, H. Kohno and T. Osawa, "Dihydrochalcones: evaluation as novel radical scavenging antioxidants," Journal of Agricultural and Food Chemistry, vol. 51, pp. 3309-3312, 2003.
[22] T. Sawa, M. Nakao, T. Akaike, K. Ono and H. Maeda, "Akylperoxyl radical-scavenging activity of various flavonoids and other phenolic compounds: implicatins for the anti-tumor-promoter effect of vegetables," Journal of Agricultural Food Chemistry, vol. 47, pp. 397- 402, 1999.
[23] M. Hajimahmoodi, N. Sadehi, B. Jannat, M. R. Oveisi, S. Madani et al., "Antioxidant activity, reducing power and total phenolic content of Iranian Olive cultivar," Journal of Biological Sciences, vol. 8(4), pp. 779-783, 2008.
[24] L. Barros, M. J. Ferreira, B. Queiros, I. C. F. R. Ferreira and P. Baptista, "Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities," Food Chemistry, vol. 103, pp. 413-419, 2007.
[25] M. S. Blois, "Antioxidant determinations by the use of a stable free radical," Nature, vol. 181, pp. 1199-1200, 1958.
[26] R. Y. Yang, S. C. S. Tsou, T. C. Lee, P. M. Hanson and P. Y. Lai, "Antioxidant Capacities and Daily Antioxidant Intake from Vegetables Consumed in Taiwan," In: Symposium on Taiwan-America Agricultural Cooperative Projects. Taichung Hsien : Agricultural Research Institute, pp. 69-77, 2005.