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Effect of L-Arginine on Neuromuscular Transmission of the Chick Biventer Cervicis Muscle

Authors: S. Asadzadeh Vostakolaei

Abstract:

In this study, the effect of L-arginine was examined at the neuromuscular junction of the chick biventer cervicis muscle. LArginine at 500 μg/ ml, decreased twitch response to electerical stimulation, and produced rightward shift of the dose- response curve for acetylcholine or carbachol. L-Arginine at 1000μg/ ml produced a strong shift to the right of the dose – response curve for acetylcholine or carbachol with a reduction in the efficacy. The inhibitory effect of L-arginine on the twitch response was blocked by caffeine (200μg/ ml). NO levels were also measured in the chick biventer cervicis muscle homogenates, using spectrophotometric method for the direct detection of NO, nitrite and nitrate. Total nitrite (nitrite + nitrate) was measured by a spectrophotometer at 540 nm after the conversion of nitrate to nitrite by copperized cadmium granules. NO levels were found to be significantly increased in concentrations 500 and 1000μg/ ml of L-arginine in comparison with the control group (p<0.001). These findings indicate a possible role of increased NO levels in the suppressive action of L-arginine on the twitch response. In addition, the results indicate that the post- junctional antagonistic action of L-arginine is probably the result of impaired sarcoplasmic reticulum (SR) Ca+2 releases.

Keywords: Chick, L-Arginine, Nitric Oxide, Skeletal muscle.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1075659

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References:


[1] M. Doherty, P.M. Smith, "Effects of caffeine ingestion on rating of perceived exertion during and after exercise: a meta-analysis", Scand J Med Sci Sports, 2005; 15 (2): 69-78.
[2] I. Gath , E.I. Closs, U. Godtel-Armbrust, S. M. Schmitt, M .Nakane and L. Wessler, "Inducible NO synthase II and neuronal NO synthase I are constitutively expressed in different structures of guinea pig skeletal muscle: implications for contractile function".FASEB, 1996, 10: 1614- 1620.
[3] J.M. Hare, "Nitric oxide and excitation-contraction coupling", J Mol Cell Cardiol 35,2003., PP.719-729.
[4] l. Kobzik, M.B. Reid, D.S. Bredt and J.S. Stamler, "Nitric oxide in skeletal muscle", 1994, Nature 372, PP.546-548.
[5] L. Kobzik, B. Stringer, J.L Balligand, M.B. Reid and J.S. Stamler, "Endothelial type nitric oxide synthase in skeletal muscle fibers: mitochondrial relationships" Biochem Biophys Res Commun, 1995, 211: 375-381.
[6] G. Maréchal and P.Gailly, "Effects of nitric oxide on the contraction of skeletal muscle", Cell Mol Life Sci (CMLF), 1999, vol.55, PP.1088- 1102.
[7] L.G. Meszaros, I.Minarovic and A. Zahradnikova, "Inhibition of the skeletal muscle ryanodine receptor calcium release channel by nitric oxide", FEBS Lett 1996;380, 49-52.
[8] M.B. Reid, "Role of nitric oxide in skeletal muscle: synthesis, distribution and functional importance", Acta Physiol Scand 1998, 162, PP. 401-409.
[9] F. Silvagno, H.Xia and D.S. Bredt, "Neuronal nitric-oxide synthase-mu, an alternatively spliced isoform expressed in differentiated skeletal muscle", J Biol Chem 1996, 271, PP.11204-11208.
[10] J.S. Stamler and G.Meissner, "Physiology of nitric oxide in skeletal muscle", Physiol Res2001, 81:209-237.
[11] M. Thompson, L.Becker, D. Bryant, G. Williams, D.Levin, L.Margraf and B.P. Giroir, "Expression of the inducible nitric oxide synthase gene in diaphragm and skeletal muscle", J Appl Physiol 1996, 81:2415-2420.
[12] W.S. Waring, J. Goudsmit, J. Marwick, D. J. Webb and S.R. Maxwell "Acute caffeine intake influences central more than peripheral blood pressure in young adults", Am J Hypertens 2003, 16: 919-924.
[13] A. Zahradnikova, I. Minarovic, R.Venema and J.G. Meszaros, "Inactivation of the cardiac ryanodine receptor calcium release channel by nitric oxide", Cell Calcium 1998, 21:447-454.