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Comparative Performance and Microbial Community of Single-phase and Two-phase Anaerobic Systems Co-Digesting Cassava Pulpand Pig Manure

Authors: P. Panichnumsin, B. K. Ahring, A. Nopharatana, P. Chaipresert

Abstract:

In this study, we illustrated the performance and microbial community of single- and two-phase systems anaerobically co-digesting cassava pulp and pig manure. The results showed that the volatile solid reduction and biogas productivity of two-phase CSTR were 66 ± 4% and 2000 ± 210 ml l-1 d-1, while those of singlephase CSTR were 59 ± 1% and 1670 ± 60 ml l-1 d-1, respectively. Codigestion in two-phase CSTR gave higher 12% solid degradation and 25% methane production than single-phase CSTR. Phylogenetic analysis of 16S rDNA clone library revealed that the Bacteroidetes were the most abundant group, followed by the Clostridia in singlephase CSTR. In hydrolysis/acidification reactor of two-phase system, the bacteria within the phylum Firmicutes, especially Clostridium, Eubacteriaceae and Lactobacillus were the dominant phylogenetic groups. Among the Archaea, Methanosaeta sp. was the exclusive predominant in both digesters while the relative abundance of Methanosaeta sp. and Methanospirillum hungatei differed between the two systems.

Keywords: Anaerobic co-digestion, Cassava pulp, Microbialdiversity, Pig manure.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1075124

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References:


[1] B. Demirel and P. Scherer, "The roles of acetotrophic and hydrogenotrophic methanogens during anaerobic conversion of biomass to methane: a review," Reviews in Environmental Science and Biotechnology, vol. 7, pp. 173-190, 2008.
[2] N. Azbar and R. E. Speece, "Two-phase, two-stage, and single-stage anaerobic process comparison," Journal of Environmental Engineering, vol. 127, pp. 240-248, 2001.
[3] S. Babel, K. Fukushi, and B. Sitanrassamee, "Effect of acid speciation on solid waste liquefaction in an anaerobic acid digester," Water Research, vol. 38, pp. 2417-2423, 2004.
[4] H. Bouallagui, O. Haouari, Y. Touhami, R. Ben Cheikh, L. Marouani, and M. Hamdi, "Effect of temperature on the performance of an anaerobic tubular reactor treating fruit and vegetable waste," Process Biochemistry, vol. 39, pp. 2143-2148, 2004.
[5] Z. Wang and C. J. Banks, "Evaluation of a two stage anaerobic digester for the treatment of mixed abattoir wastes," Process Biochemistry, vol. 38, pp. 1267-1273, 2003.
[6] G. l. m. Yilmazer and O. Yenigon, "Two-phase anaerobic treatment of cheese whey," Water Science and Technology, vol. 40, pp. 289-295, 1999.
[7] T. C. Zhang and T. Noike, "Comparison of one-phase and two-phase anaerobic digestion in characteristics of substrate degradation and bacterial population level," Water Science and Technology, vol. 23, pp. 1157-1166, 1991.
[8] W. A. Joubert and T. J. Britz, "The effect of pH and temperature manipulation on metabolite composition during acidogenesis in a hybrid anaerobic digester," Applied Microbiology and Biotechnology, vol. 24, pp. 253-258, 1986.
[9] J. Massanet-Nicolau, R. Dinsdale, and A. Guwy, "Hydrogen production from sewage sludge using mixed microflora inoculum: Effect of pH and enzymatic pretreatment," Bioresource Technology, vol. 99, pp. 6325- 6331, 2008.
[10] H. Q. Yu, X. J. Zheng, Z. H. Hu, and G. W. Gu, "High-rate anaerobic hydrolysis and acidogenesis of sewage sludge in a modified upflow reactor," in Water Science and Technology. vol. 48, 2003, pp. 69-75.
[11] S. T. Cassini, M. C. E. Andrade, T. A. Abreu, R. Keller, and R. F. Goncalves, "Alkaline and acid hydrolytic processes in aerobic and anaerobic sludges: effect on total EPS and fractions," in 4th International Symposium on Anaerobic Digestion of Solid Waste, Copenhagen, 2005.
[12] Y. Yu, B. Park, and S. Hwang, "Co-digestion of lignocellulosics with glucose using thermophilic acidogens," Biochemical Engineering Journal, vol. 18, pp. 225-229, 2004.
[13] N. Paepatung, P. Kullavanijaya, O. Laopitinun, A. Nopharatana, W. Songkasisri, P. Chaiprasert, and M. Leetochavalit, "Current status of biomass potential and biogas technologies in Thailand," in International symposium in science and technology at Kansai University, Collaboration between ASEAN countries in environment and life science Osaka, Japan, 2007.
[14] P. Panichnumsin, A. Noppharatana, B. K. Ahring, and P. Chaiprasert, "Anaerobic Co-digestion of Cassava Pulp and Pig manure: Effects of Waste Ratio and Inoculum Substrate Ratio," in SEE 2006 Sustainable Energy and Environment Technology and Policy Innovations, 1. Bangkok, Thailand, 2006, pp. 932-937.
[15] F. J. Callaghan, D. A. J. Wase, K. Thayanithy, and C. F. Forster, "Continuous co-digestion of cattle slurry with fruit and vegetable wastes and chicken manure," Biomass and Bioenergy, vol. 22, pp. 71- 77, 2002.
[16] J. Cheon, T. Hidaka, S. Mori, H. Koshikawa, and H. Tsuno, "Applicability of random cloning method to analyze microbial community in full-scale anaerobic digesters," Journal of Bioscience and Bioengineering, vol. 106, pp. 134-140, 2008.
[17] I. C. S. Duarte, L. L. Oliveira, N. K. D. Saavedra, F. Fantinatti- Garboggini, V. M. Oliveira, and M. B. A. Varesche, "Evaluation of the microbial diversity in a horizontal-flow anaerobic immobilized biomass reactor treating linear alkylbenzene sulfonate," Biodegradation, vol. 19, pp. 375-385, 2008.
[18] M. Lee, T. Hidaka, W. Hagiwara, and H. Tsuno, "Comparative performance and microbial diversity of hyperthermophilic and thermophilic co-digestion of kitchen garbage and excess sludge," Bioresource Technology, vol. 100, pp. 578-585, 2009.
[19] H. Wang, A. Lehtomaki, K. Tolvanen, J. Puhakka, and J. Rintala, "Impact of crop species on bacterial community structure during anaerobic co-digestion of crops and cow manure," Bioresource Technology, vol. 100, pp. 2311-2315, 2009.
[20] APHA, Standard methods for the examination of water and waste water, 19 ed. Washington, D.C., USA: American Public Health Association, American Water Works Association, and Water Environment Federation, 1995.
[21] D. S. Mladenovska Z, Ahring BK., "Anaerobic digestion of manure and mixture of manure with lipids: biogas reactor performance and microbial community analysis.," Water Science and Technology, vol. 48, pp. 271-8, 2003.
[22] J. Godon, E. Zumstein, P. Dabert, F. Habouzit, and R. Moletta, "Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis," Appl. Environ. Microbiol., vol. 63, pp. 2802-2813, 1997.
[23] M. Munson, D. Nedwell, and T. Embley, "Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh," Appl. Environ. Microbiol., vol. 63, pp. 4729-4733, 1997.
[24] J. Sambrook, E. F. Fritsch, and T. Maniatis, Molecular cloning a laboratory manual, 2nd ed.: Cold Spring Harbor Laboratory Press, 1989.
[25] U. Nubel, B. Engelen, A. Felske, J. Snaidr, A. Wieshuber, R. Amann, W. Ludwig, and H. Backhaus, "Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis," J. Bacteriology, vol. 178, pp. 5636-5643, 1996.
[26] Z. Yu and M. Morrison, "Comparisons of Different Hypervariable Regions of rrs Genes for Use in Fingerprinting of Microbial Communities by PCR-Denaturing Gradient Gel Electrophoresis " Applied and Environmental Microbiology, , vol. 70, pp. 4800-4806, 2004.
[27] R. I. Amann, W. Ludwig, and K. H. Schleifer, "Phylogenetic identification and in situ detection of individual microbial cells without cultivation. ," Microbiology Reviews, vol. 59, pp. 143-169, 1995.
[28] L. Raskin, L. K. Poulsen, D. R. Noguera, B. E. Rittmann, and D. A. Stahl, "Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization," Appl Environ Microbiol., vol. 60, pp. 1241-1248, 1994
[29] W. V. Sigler, C. Miniaci, and J. Zeyer, "Electrophoresis time impacts the denaturing gradient gel electrophoresis-based assessment of bacterial community structure," Journal of Microbiological Methods, vol. 57, pp. 17-22, 2004.
[30] R. Slepecky and H. Hemphill, "The Genus BacillusÔÇöNonmedical," in The Prokaryotes, 2006, pp. 530-562.
[31] J. Wiegel, R. Tanner, and F. A. Rainey, "An introduction to the family Clostridiaceae," in The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3rd ed, M. Dworkin, Ed. New York, 2005.
[32] R. Sleat and R. Mah, Hydrolytic bacteria In Anaerobic digestion of biomass. London: Elsevier Applied Science, 1987.
[33] P. Peu, H. Brugère, A. M. Pourcher, M. Kérourédan, J. J. Godon, J. P. Delgenès, and P. Dabert, "Dynamics of a Pig Slurry Microbial Community during Anaerobic Storage and Management " Applied and Environmental Microbiology, vol. 72, pp. 3578-3585, 2006.
[34] R. Snell-Castro, J.-J. Godon, J.-P. Delgenes, and P. Dabert, "Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis," FEMS microbiology ecology, vol. 52, pp. 229-42, 2005.
[35] L. R. Lynd, P. J. Weimer, W. H. Van-Zyl, and I. S. Pretorius, "Microbial Cellulose Utilization: Fundamentals and Biotechnology " Microbiology and Molecular Biology Reviews, vol. 66, pp. 506-577, 2002.
[36] C. Matthies, C. H. Kuhner, G. Acker, and H. L. Drake, "Clostridium uliginosum sp. nov., a novel acid-tolerant, anaerobic bacterium with connecting filaments," International Journal of Systematic and Evolutionary Microbiology, vol. 51, pp. 1119-1125, 2001.
[37] M. De Angelis, S. Siragusa, M. Berloco, L. Caputo, L. Settanni, G. Alfonsi, M. Amerio, A. Grandi, A. Ragni, and M. Gobbetti, "Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding," Research in Microbiology, vol. 157, pp. 792-801, 2006.
[38] B. G. Wiese, W. Strohmar, F. A. Rainey, and H. Diekmann, "Lactobacillus panis sp. nov., from Sourdough with a Long Fermentation Period," International Journal System Bacteriology vol. 46 pp. 449-453, 1996.
[39] N. F. Ye, F. Lu, L. M. Shao, J. J. Godon, and P. J. He, "Bacterial community dynamics and product distribution during pH-adjusted fermentation of vegetable wastes," Journal of Applied Microbiology, vol. 103, pp. 1055-1065, 2007.
[40] J. Kuever and B. Schink, "Syntrophus Mountfort, Brulla, Krumholz and Bryant 1984, 216 VP," in Bergey-s Manual® of Systematic Bacteriology, 2005, pp. 1033-1035.
[41] B. Jackson, Bhupathiraju VK, Tanner RS, Woese CR, and M. McInerney, "Syntrophus aciditophicus sp. nov., a new anaerobic bacterium that degrades fatty acids and benzoate in syntrophic association with hydrogen-using microorganisms," Arch Microbiol, vol. 171, pp. 107-114, 1999.
[42] Liu Y, Balkwill DL, Aldrich HC, Drake GR, and B. DR, "Characterization of the anaerobic propionate-degrading syntrophs Smithella propionica gen. nov., sp. nov. and Syntrophobacter wolinii," Int J Syst Bacteriol, vol. 49, pp. 545-556, 1999.
[43] S. Zinder, "Physiological ecology of methanogens," in Methanogenesis, J. Ferry, Ed. New York: Chapman&Hall, 1993, pp. 128-206.
[44] S. E. Lowe, M. K. Jain, and J. G. Zeikus, "Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, salinity, or substrates.," Microbiology Reviews, vol. 57, pp. 451-509, 1993.
[45] P. L. McCarty and D. P. Smith, "Anaerobic waste water treatment," Environmental Science and Technology, vol. 20, pp. 1200-1206, 1986.
[46] K. D. McMahon, P. G. Stroot, R. I. Mackie, and L. Raskin, "Anaerobic codigestion of municipal solid waste and biosolids under various mixing conditions--II: microbial population dynamics," Water Research, vol. 35, pp. 1817-1827, 2001.