Pervasiveness of Aflatoxin in Peanuts Growing in the Area of Pothohar, Pakistan

Authors: Mateen Abbas, Abdul Muqeet Khan, Muhammad Rafique Asi, Javed Akhtar

Abstract:

Mycotoxin (aflatoxins) contamination of peanuts is a great concern for human health. A total of 72 samples of unripe, roasted, and salty peanuts were collected randomly from Pothohar plateau of Pakistan for the assessment of aflatoxin. Samples were dried, ground and extracted by acetonitrile (84%). The filtered extracts were cleaned up by MycoSep-226 and analyzed by high performance liquid chromatography with flourescence detector. Quantification limit of Aflatoxin was 1 μg/kg and 70% Recovery was observed in spiked samples in the range 1–10 μg/kg. The screening of mycotoxins indicated that aflatoxins were present in most of the samples being detected in 82%, in concentrations from 14.25 μg/kg to 98.80 μg/kg. Optimal conditions for mycotoxin production and fungal growth are frequently found in the crop fields as well as in store houses. Human exposure of such toxin can be controlled by pointed out such awareness and implemented the regulations.

Keywords: Aflatoxin, HPLC, Pakistan, Peanuts, Punjab

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1074714

References:

[1] M. A. Shad, P. Humayun, N. Haq, K. Hyder and A. U. Muhammad, "Evaluation of biochemical and phytochemical Composition of some groundnut varieties Grown in arid zone of Pakistan", Pak. J. Bot., vol. 41, no. 6, pp. 2739-2749, 2009.
[2] N. Hussain and C. R. Ahmed, "Problems of groundnut cultivation in the representative areas of Punjab," Planning and Evaluation cell, Agriculture department, Lahore, 1984, pp. 1-14.
[3] R. V. Bhat and S. Vashanti, "Occurrence of aflatoxins and its economic impact on human nutrition and animal feed," The New Regulation, Agricultural Development, vol. 23, pp. 50-56, 1999.
[4] H. Njapau, E. M. Muzungaile and R. C. Changa, "The effect of village processing techniques on the content of aflatoxins in corn and peanuts in Zambia," Journal of the Science of Food and Agriculture, vol. 76, pp. 450-456, 1998.
[5] D. McDonald and C. Harkness, "Growth of Aspergillus flavus and production of aflatoxin in groundnuts, Part IV," Tropical Science, vol. 4, pp. 12-27, 1964.
[6] T. D. Phillips, "Detection and Decontamination of aflatoxincontaminated food products," in J. H. Williams, D. G. Cummins, G. Hutto and A. King (Eds.), Impacts and scientific advances through collaborative research on peanut CRSP, 1997, pp. 117-130.
[7] S. A. Bankole and A. Adebanjo, "Mycotoxins in food in West Africa: current situation and possibilities of controlling it," African Journal of Biotechnology, vol. 2, no. 9, 254-263, 2003.
[8] N. Magan, V. Sanchis and D. Aldred, "Role of spoilage fungi in seed deterioration," in D. K. Aurora (Ed.), Fungal biotechnology in agricultural, food and environmental applications, Chapter 28, pp. 311- 323, 2004.
[9] Codex Alimentarius Commission. "Joint FAO/WHO food standards programme," Codex committee on food additives and contaminants. Thirty-third session. Hague, Netherlands: CODEX, 2001.
[10] R. G. D. Steel, J. H. Torrie and D. A. Dickey, "Principles and procedures of statistics," Mc Graw Hill Book Co., Inc., New York, 1997.
[11] A. Brudzynski, W. Van Pee and W. Kornazewski, "The occurrence of aflatoxin B1 in peanuts, corn and dried cassava sold at the local market in Kinshasa, Zaire: its coincidence with high hepatoma morbidity among the population," Zeszyty Problemowe Postepow Nauk Rolniczych, vol. 189, pp. 113-115, 1977.
[12] N. Barro, C. A. Quattara, P. A. Nikiema, A. S. Quattara and A.S. Traore, "Microbial quality assessment of some street food Widely consumed in Ouagadougou, Burkina Faso," Sante, vol. 12, pp. 369-374, 2002.
[13] V. V. Garcia, "An overview of peanut utilization in the Philippines," in T. C. Yam and C. Tan (Eds.), Trends in food product development, Singapore: Singapore Institute of Food Science and Technology, pp. 21- 26, 1989.
[14] C. K. Mutegi, H. K. Ngugi, S. L. Hendriks and R. B. Jones, "Prevalence and factors associated with aflatoxin contamination of peanuts from Western Kenya," International Journal of Food Microbiology, vol. 130, no. 1, pp. 27-34, 2009.
[15] I. Kamika and L. L. Takoy, "Natural occurrence of Aflatoxin B1 in peanut collected from Kinshasa Democratic Republic of Congo," Food Control, vol. 22, pp. 1760-1764, 2011.
[16] S. A. Bankole, A. A. Adenusi, O. S. Lawal and O. O. Adesanya, "Occurrence of aflatoxin B1 in food products derivable from ÔÇÿegusi- melon seeds consumed in southwestern Nigeria," Food control, vol. 21, pp. 974-976, 2010.
[17] H. E. Ok, H. L. Kim, W. B. Shim, H. Lee, D. H. Bae and D. H. Chung, "Natural occurrence of Afaltoxin B1 in the marketed foods and risk estimates of dietary exposure in Koreans," Journal of Food Protection, vol. 70, no. 12, pp. 2824-2828, 2007.
[18] S. Egal, A. Hounsa, Y. Y. Gong, P. C. Turner, C. P. Wild, A. Hall, "Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa," International Journal of Food microbiology, vol. 104, pp. 215-234, 2005.
[19] L. Shundo, S. A. Navas, L. C. A. Lamardo, V. Ruvieri, and M. Sabino, "Estimate of aflatoxinM1exposure in milk and occurrence in Brazil," Food Control, vol. 20, pp. 655-657, 2009.
[20] E. Razzazi-Fazeli, C. T. Noviandi, S. Porasuphatana, A. Agus and J. Bohm, "A survey of aflatoxins and B1 and total afaltoxin contamination in baby food, peanut and corn products sold at retail in Indonesia analysed by ELSA and HPLC," Mycotoxin Research, vol. 20, pp. 51-58, 2004.
[21] J. H. Williams, T. D. Phillips, P. E. Jolly, J. K. Stiles, C. M. Jolly and D. Aggarwal, "Human aflatoxincosis in developing countries: a review of toxicology, exposure, potential health consequences and interventions," American Society and Clinical Nutrition, vol. 80, no.5, pp. 1106-1122, 2004.