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Influence of Laser Treatment on the Growth of Sprouts of Different Wheat Varieties

Authors: N. Bakradze, N. Gagelidze, T. Dumbadze, L. Amiranashvili, A. D. L. Batako


Cereals are considered as a strategic product in human life and their demand is increasing with the growth of world population. Increasing wheat production is important for the country. One of the ways to solve the problem is to develop and implement new, environmentally and economically acceptable technologies. Such technologies include pre-sowing treatment of seed with a laser and associative nitrogen-fixing bacteria - Azospirillum brasilense. In the region there are the wheat varieties - Dika and Lomtagora, which are among the most common in Georgia. Dika is a frost-resistant wheat, with a high ability to adapt to the environment, resistant to falling and it is sown in highlands. Lomtagora 126 differs with its winter and drought resistance, and it has a great ability to germinate. Lomtagora is characterized by a strong root system and a high budding capacity. It is an early variety, fall-resistant, easy to thresh and suitable for mechanized harvesting with large and red grains. This paper presents some preliminary experimental results where a continuous CO2 laser with a power of 25-40 W was used to radiate grains at a flow rate of 10 and 15 cm/sec. The treatment was carried out on grains of the Triticum aestivum L. var. Lutescens (local variety name - Lomtagora 126), and Triticum carthlicum Nevski (local variety name - Dika). Here the grains were treated with A. brasilense isolate (108-109 CFU/ml), which was isolated from the rhizosphere of wheat. It was observed that the germination of the wheat was not significantly influenced by either laser or bacteria treatment. The results of our research show that combined treatment with laser and A. brasilense significantly influenced the germination of wheat. In the case of the Lomtagora 126 variety, grains were exposed to the beam on a speed of 10 cm/sec, only slightly improved the growth for 38-day seedlings, in case of exposition of grains with a speed of 15 cm/sec - by 23%. Treatment of seeds with A. brasilense in both exposed and non-exposed variants led to an improvement in the growth of seedlings, with A. brasilense alone - by 22%, and with combined treatment of grains - by 29%. In the case of the Dika variety, only exposure led to growth by 8-9%, and the combined treatment - by 10-15%, in comparison with the control variant. Superior effect on growth of seedlings of different varieties was achieved with the combinations of laser treatment on grains in a beam of 15 cm/sec (radiation power 30-40 W) and in addition of A. brasilense - nitrogen fixing bacteria. Therefore, this is a promising application of A. brasilense as active agents of bacterial fertilizers due to their ability of molecular nitrogen fixation in cereals in combination with laser irradiation: choosing a proper strain gives a good ability to colonize roots of agricultural crops, providing a high nitrogen-fixing ability and the ability to mobilize soil phosphorus, and laser treatment stimulates natural processes occurring in plant cells, will increase the yield.

Keywords: laser treatment, Azospirillum brasilense, seeds, wheat varieties, Lomtagora, Dika

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[1] L. Ujmajuridze, Ts. Samadashvili, and G. Chkhutiashvili, “The strategy of wheat production and its role in the state independence of Georgia,” Bulletin of the Georgian Academy of Agricultural Sciences, vol. 37, no. 1, pp. 10–13, June 2017.
[2] E.N. Sinskaya, “Historical geography of cultivated flora (at the dawn of agriculture),” Leningrad: Kolos, 1969.
[3] D. Bedoshvili, M. Mosulishvili, M. Merabishvili, G. Chkhutiashvili, M. Chokheli, and N. Ustiashvili, “Heritage wheats of Georgia”, XXIV International Scientific and Practical Conference: International Trends in Science and Technology, Poland, 2020, pp. 3–5.
[4] T. Kaukhchishvili, “Herodote's notes about Georgia,” Tbilisi: Academy of Sciences of Georgian SSR, 1960.
[5] B. Bolghashvili, “Management of the technological quality of winter wheat grain,” Bulletin of the Georgian Academy of Agricultural Sciences, vol. 37, no. 1, pp. 14–17, June 2017.
[6] M. Gogniashvili, P. Naskidashvili, D. Bedoshvili, A. Kotorashvili, N. Kotaria, and T. Beridze, “Complete chloroplast DNA sequences of Zanduri wheat (Triticum spp.),” Genetic Resources and Crop Evolution, vol. 62, pp. 1269–1277, March 2015.
[7] Ts. Samadashvili, G. Chkhartishvili, and N. Bendianishvili, “Georgian Soft Wheat Varieties – Yield and Propagation Opportunities,” Agrarian Georgia, vol. 3, pp. 16–18, Mar. 2017.
[8] C. Hernández-Aguilar, A. Domínguez-Pacheco, A. Cruz-Orea, A. Podleśna, R. Ivanov, A. C. Carballo, M. C. Pérez Reyes, G. Sánchez Hernández, R. Z. Bautista and J. L. López-Bonilla, “Laser biostimulation in seeds and plants,” Gayana Bot., vol.73 no.1, pp. 132–149, June 2016.
[9] L. Ferdosizadeh, S.A. Sadat-Noori, N. Zare, S. Saghafi, “Assessment laser pretreatments on germination and yield of wheat (Triticum Aestivum L.) under salinity stress,” World Journal of Agricultural Research, vol. 1, no. 1, pp. 5–9, 2013.
[10] G. G. Piccinin, A. L. Braccini, C. A. Scapim, A. K. Suzukawa, L. G. M. Dan, and F. B. Godinho, “Agronomic performance of maize in response to seed inoculation with Azospirillum brasilense associated with nitrogen doses and bioregulator,” Journal of Food, Agriculture & Environment, vol.13, no. 3&4, pp. 67–73, Oct. 2015.
[11] Y. Pii, T. Mimmo, N. Tomasi, R. Terzano, S. Cesco, and C. Crecchio, “Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process”, Biol Fertil Soils, 51, 4, 403–415, Jan. 2015.
[12] O. Fukami, P. Cerezini, M. Hungria, “Azospirillum: benefits that go far beyond biological nitrogen fixation”. AMB Express, vol. 73, no. 8, 1–12, May 2018.
[13] G. G. Piccinin, A. L. Braccini, L. G.M. Dan, C. A. Scapim, Th. T. Ricci, and G. L. Bazo, “Efficiency of seed inoculation with Azospirillum brasilense on agronomic characteristics and yield of wheat,” J. Industrial Crops and Products, vol. 43, 393–397, May 2013.
[14] J. I. Baldani, V.L.D. Baldani, “History on the biological nitrogen fixation research in graminaceous plants: special emphasis on the Brazilian experience,” An Acad Bras Cienc, vol. 77, no. 3, pp. 549–79, Sept. 2005.
[15] A.Torbica, J. Mastilović, “Influence of different factors on wheat proteins quality,” Food Processing, Quality and Safety, vol. 35, no. 2, pp. 47–52, Apr. 2008.
[16] N. G. Halford, T. Y. Curtis, Zh. Chen, and J. Huang, “Effects of abiotic stress and crop management on cereal grain composition: implications for food quality and safety,” Journal of Experimental Botany, vol. 66, no. 5, pp. 1145–1156, March 2015.
[17] C. Creus, R. Sueldo, C. Barassi, “Azospirillum inoculation in pregerminating wheat seeds,” Canadian Journal of Microbiology, vol. 42, no. 1, pp. 83–86, Jan. 1996