Commenced in January 2007
Paper Count: 31515
Investigating the Formation of Nano-Hydroxyapatite on a Biocompatible and Antibacterial Cu/Mg-Substituted Bioglass
Abstract:Multifunctional bioactive glasses (BGs) are designed with a focus on the provision of bactericidal and biological properties desired for angiogenesis, osteogenesis, and ultimately potential applications in bone tissue engineering. To achieve these, six sol-gel copper/magnesium substituted derivatives of 58S-BG, i.e. a mol% series of 60SiO2-4P2O5-5CuO-(31-x) CaO/xMgO (where x=0, 1, 3, 5, 8, and 10), were synthesized. Afterwards, the effect of MgO/CaO substitution on the in vitro formation of nano-hydroxyapatite (HA), osteoblast-like cell responses and BGs antibacterial performance were studied. During the BGs synthesis, the elimination of nitrates was achieved at 700 °C that prevented the BGs crystallization and stabilized the obtained dried gels. The structural and morphological evaluations were performed with X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). These characterizations revealed that Cu-substituted 58S-BG consisting of 5 mol% MgO (BG-5/5) slightly had retarded the formation of HA. In addition, Cu-substituted 58S-BGs consisting 8 mol% and 10 mol% MgO (BG-5/8 and BG-5/10) displayed lower bioactivity probably due to the lower ion release rate of Ca–Si into the simulated body fluid (SBF). The determination of 3-(4, 5 dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and alkaline phosphate (ALP) activities proved that the highest values of both differentiation and proliferation of MC3T3-E1 cells can be obtained from a 5 mol% MgO substituted BG, while the over addition of MgO (8 mol% and 10 mol%) decreased the bioactivity. Furthermore, these novel Cu/Mg-substituted 58S-BGs displayed antibacterial effect against methicillin-resistant Staphylococcus aureus bacteria. Taken together, the results suggest the equally-substituted BG-5/5 (i.e. the one consists of 5 mol% of both CuO and MgO) as a promising candidate for bone tissue engineering, among all newly designed BGs in this work, owing to its desirable cell proliferation, ALP activity and antibacterial properties.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.3300508Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 431
 K. H. Karlsson, L. Hupa, Thirty-five years of guided tissue engineering, J. Non. Cryst. Solids. 354 (2008) 717–721.
 G. Dickson, F. Buchanan, D. Marsh, E. Harkin-Jones, U. Little, M. McCaigue, Orthopaedic tissue engineering and bone regeneration, Technol. Heal. Care. 15 (2007) 57–67.
 B. Sharma, S. Varghese, Progress in orthopedic biomaterials and drug delivery, Drug Deliv. Transl. Res. 6 (2016) 75–76.
 T. Kokubo, Nihon Medikaru Materiaru Kabushiki Kaisha., Bioceramics and their clinical applications, Woodhead Pub. and Maney Pub. on behalf of Institute of Materials, Minerals & Mining, 2008.
 M. Laczka, K. Cholewa-Kowalska, A. M. Osyczka, Bioactivity and osteoinductivity of glasses and glassceramics and their material determinants, Ceram. Int. 42 (2016) 14313–14325.
 L. L. Hench, The story of Bioglass®, J. Mater. Sci. Mater. Med. 17 (2006) 967–978.
 P. Sepulveda, J. R. Jones, L. L. Hench, Characterization of melt-derived 45S5 and sol-gel-derived 58S bioactive glasses, J. Biomed. Mater. Res. 58 (2001) 734–740.
 D. Arcos, D. C. Greenspan, M. Vallet-Regí, A new quantitative method to evaluate the in vitro bioactivity of melt and sol-gel-derived silicate glasses, J. Biomed. Mater. Res. Part A. 65A (2003) 344–351.
 R. Ravarian, F. Moztarzadeh, M. S. Hashjin, S. M. Rabiee, P. Khoshakhlagh, M. Tahriri, Synthesis, characterization and bioactivity investigation of bioglass/hydroxyapatite composite, Ceram. Int. 36 (2010) 291–297.
 A. Moghanian, S. Firoozi, M. Tahriri, Synthesis and in vitro studies of sol-gel derived lithium substituted 58S bioactive glass, Ceram. Int. 43 (2017) 12835–12843.
 A. Moghanian, S. Firoozi, M. Tahriri, Characterization, in vitro bioactivity and biological studies of sol-gel synthesized SrO substituted 58S bioactive glass, Ceram. Int. 43 (2017).
 J. Ma, C. Z. Chen, D. G. Wang, X. Shao, C. Z. Wang, H. M. Zhang, Effect of MgO addition on the crystallization and in vitro bioactivity of glass ceramics in the CaO–MgO–SiO2–P2O5 system, Ceram. Int. 38 (2012) 6677–6684.
 A. Moghanian, A. Sedghi, A. Ghorbanoghli, E. Salari, The effect of magnesium content on in vitro bioactivity, biological behavior and antibacterial activity of sol–gel derived 58S bioactive glass, Ceram. Int. (2018).
 A. M. El-Kady, A. F. Ali, R. A. Rizk, M. M. Ahmed, Synthesis, characterization and microbiological response of silver doped bioactive glass nanoparticles, Ceram. Int. 38 (2012) 177–188.
 C. Wu, Y. Zhou, M. Xu, P. Han, L. Chen, J. Chang, Y. Xiao, Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity, Biomaterials. 34 (2013) 422–433.
 R. L. Du, J. Chang, S. Y. Ni, W. Y. Zhai, J. Y. Wang, Characterization and in vitro Bioactivity of Zinc-containing Bioactive Glass and Glass-ceramics, J. Biomater. Appl. 20 (2006) 341–360.
 N. Kilcup, U. Werner-Zwanziger, E. Tonkopi, D. Boyd, Unanticipated stabilization of zinc-silicate glasses by addition of lanthanum: Implications for therapeutic inorganic ion delivery systems, J. Non. Cryst. Solids. 429 (2015) 83–92.
 J. Ma, C. Z. Chen, D. G. Wang, J. H. Hu, Synthesis, characterization and in vitro bioactivity of magnesium-doped sol–gel glass and glass-ceramics, Ceram. Int. 37 (2011) 1637–1644.
 A. Hoppe, B. Sarker, R. Detsch, N. Hild, D. Mohn, W. J. Stark, A. R. Boccaccini, In vitro reactivity of Sr-containing bioactive glass (type 1393) nanoparticles, J. Non. Cryst. Solids. 387 (2014) 41–46.
 F. Baino, G. Novajra, V. Miguez-Pacheco, C. Vitale-Brovarone, Bioactive glasses: Special applications outside the skeletal system, J. Non. Cryst. Solids. 432 (2016) 15–30.
 M. E. Maguire, J. A. Cowan, Magnesium chemistry and biochemistry, BioMetals. 15 (2002) 203–210.
 D. H. H. M. Viering, J. H. F. de Baaij, S. B. Walsh, R. Kleta, D. Bockenhauer, Genetic causes of hypomagnesemia, a clinical overview, Pediatr. Nephrol. 32 (2017) 1123–1135.
 S. Castiglioni, A. Cazzaniga, W. Albisetti, J. Maier, Magnesium and Osteoporosis: Current State of Knowledge and Future Research Directions, Nutrients. 5 (2013) 3022–3033.
 M. M. Belluci, T. Schoenmaker, C. Rossa-Junior, S. R. Orrico, T. J. de Vries, V. Everts, Magnesium deficiency results in an increased formation of osteoclasts, J. Nutr. Biochem. 24 (2013) 1488–1498.
 S. Bernick, G. F. Hungerford, Effect of Dietary Magnesium Deficiency on the Bones and Teeth of Rats, J. Dent. Res. 44 (1965) 1317–1324.
 R. K. Rude, H. E. Gruber, L. Y. Wei, A. Frausto, B. G. Mills, Magnesium Deficiency: Effect on Bone and Mineral Metabolism in the Mouse, Calcif. Tissue Int. 72 (2003) 32–41.
 M. A. Saghiri, A. Asatourian, J. Orangi, C. M. Sorenson, N. Sheibani, Functional role of inorganic trace elements in angiogenesis—Part II: Cr, Si, Zn, Cu, and S, Crit. Rev. Oncol. Hematol. 96 (2015) 143–155.
 A. Nasulewicz, A. Mazur, A. Opolski, Role of copper in tumour angiogenesis—clinical implications, J. Trace Elem. Med. Biol. 18 (2004) 1–8.
 G. Hu, Copper stimulates proliferation of human endothelial cells under culture, J. Cell. Biochem. 69 (1998) 326–335.
 J. Li, D. Zhai, F. Lv, Q. Yu, H. Ma, J. Yin, Z. Yi, M. Liu, Preparation of copper-containing bioactive glass/eggshell membrane nanocomposites for improving angiogenesis, antibacterial activity and wound healing, Acta Biomater. (2016).
 J. Ye, J. He, C. Wang, K. Yao, Z. Gou, Copper-containing mesoporous bioactive glass coatings on orbital implants for improving drug delivery capacity and antibacterial activity, Biotechnol. Lett. 36 (2014) 961–968.
 J. Pérez-Pariente, A. F. Balas, M. Vallet-Regí*, Surface and Chemical Study of SiO2•P2O5•CaO•(MgO) Bioactive Glasses, Chem. Mater. 12 (2000) 750–755.
 J. Ma, C. Z. Chen, D. G. Wang, Y. Jiao, J. Z. Shi, Effect of magnesia on the degradability and bioactivity of sol–gel derived SiO2–CaO–MgO–P2O5 system glasses, Colloids Surfaces B Biointerfaces. 81 (2010) 87–95.
 M. Vallet-Regí, A. J. Salinas, J. Román, M. Gil, L. L. Hench, Effect of magnesium content on the in vitro bioactivity of CaO-MgO-SiO2-P2O5 sol-gel glasses, J. Mater. Chem. 9 (1999) 515–518.
 E. Dietrich, H. Oudadesse, In vitro bioactivity of melt‐derived glass 46S6 doped with magnesium, J. Biomed. 88 (2009) 1087–1096.
 D. Bellucci, A. Sola, R. Salvatori, A. Anesi, L. Chiarini, V. Cannillo, Role of magnesium oxide and strontium oxide as modifiers in silicate-based bioactive glasses: Effects on thermal behaviour, mechanical properties and in-vitro bioactivity, Mater. Sci. Eng. C. 72 (2017) 566–575.
 S. J. Watts, R. G. Hill, M. D. O’Donnell, R. V. Law, Influence of magnesia on the structure and properties of bioactive glasses, J. Non. Cryst. Solids. 356 (2010) 517–524.
 M. Prabhu, K. Kavitha, P. Manivasakan, V. Rajendran, P. Kulandaivelu, Synthesis, characterization and biological response of magnesium-substituted nanobioactive glass particles for biomedical applications, Ceram. Int. 39 (2013) 1683–1694.
 M. Erol, A. Özyuguran, Ö. Çelebican, Synthesis, Characterization, and In Vitro Bioactivity of Sol-Gel-Derived Zn, Mg, and Zn-Mg Co-Doped Bioactive Glasses, Chem. Eng. Technol. 33 (2010) 1066–1074.
 J. S. Moya, A. P. Tomsia, A. Pazo, C. Santos, F. Guitin, In vitro formation of hydroxylapatite layer in a MgO-containing glass, J. Mater. Sci. Mater. Med. 5 (1994) 529–532.
 A.. Salinas, J. Román, M. Vallet-Regi, J. Oliveira, R.. Correia, M.. Fernandes, In vitro bioactivity of glass and glass-ceramics of the 3CaO•P2O5–CaO•SiO2–CaO•MgO•2SiO2 system, Biomaterials. 21 (2000) 251–257.
 A. Saboori, M. Rabiee, F. Moztarzadeh, M. Sheikhi, M. Tahriri, M. Karimi, Synthesis, characterization and in vitro bioactivity of sol-gel-derived SiO2–CaO–P2O5–MgO bioglass, Mater. Sci. Eng. C. 29 (2009) 335–340.
 X. Wang, X. Li, A. Ito, Y. Sogo, Synthesis and characterization of hierarchically macroporous and mesoporous CaO–MO–SiO2–P2O5 (M=Mg, Zn, Sr) bioactive glass scaffolds, Acta Biomater. 7 (2011) 3638–3644.
 A. Balamurugan, G. Balossier, J. Michel, S. Kannan, H. Benhayoune, A.H.S. Rebelo, J.M.F. Ferreira, Sol gel derived SiO2-CaO-MgO-P2O5 bioglass system—Preparation andin vitro characterization, J. Biomed. Mater. Res. Part B Appl. Biomater. 83B (2007) 546–553.
 R. Namba, M. Inacio, E. Paxton, Risk factors associated with deep surgical site infections after primary total knee arthroplasty: an analysis of 56,216 knees, JBJS. (2013).
 K. Yuan, Y. Chan, K. Kung, Comparison of osseointegration on various implant surfaces after bacterial contamination and cleaning: a rabbit study., Int. J. (2014).
 J. Kolmas, E. Groszyk, D. Kwiatkowska-Różycka, Substituted hydroxyapatites with antibacterial properties., Biomed Res. Int. 2014 (2014) 178123.
 D. Campoccia, L. Montanaro, C. R. Arciola, A review of the biomaterials technologies for infection-resistant surfaces, Biomaterials. 34 (2013) 8533–8554.
 T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi, T. Yamamuro, Solutions able to reproducein vivo surface-structure changes in bioactive glass-ceramic A-W3, J. Biomed. Mater. Res. 24 (1990) 721–734.
 H. M. Elgendy, M. E. Norman, A. R. Keaton, C. T. Laurencin, Osteoblast-like cell (MC3T3-E1) proliferation on bioerodible polymers: an approach towards the development of a bone-bioerodible polymer composite material, Biomaterials. 14 (1993) 263–269.
 Y. Gotoh, K. Hiraiwa, M. Nagayama, In vitro mineralization of osteoblastic cells derived from human bone., Bone Miner. 8 (1990) 239–50.
 C. E. Yellowley, Z. Li, Z. Zhou, C. R. Jacobs, H. J. Donahue, Functional Gap Junctions Between Osteocytic and Osteoblastic Cells, J. Bone Miner. Res. 15 (2010) 209–217.
 S. Hu, J. Chang, M. Liu, C. Ning, Study on antibacterial effect of 45S5 Bioglass®, J. Mater. Sci. Mater. Med. 20 (2009) 281–286.
 S. Hu, C. Ning, Y. Zhou, L. Chen, K. Lin, J. Chang, Antibacterial activity of silicate bioceramics, J. Wuhan Univ. Technol. Sci. Ed. 26 (2011) 226–230.
 R. L. Siqueira, O. Peitl, E. D. Zanotto, Gel-derived SiO2–CaO–Na2O–P2O5 bioactive powders: Synthesis and in vitro bioactivity, Mater. Sci. Eng. C. 31 (2011) 983–991.
 D. Xiao, Z. Tan, Y. Fu, K. Duan, X. Zheng, X. Lu, Hydrothermal synthesis of hollow hydroxyapatite microspheres with nano-structured surface assisted by inositol hexakisphosphate, Ceram. Int. (2014).
 M. Mozafari, F. Moztarzadeh, M. Tahriri, Investigation of the physico-chemical reactivity of a mesoporous bioactive SiO2–CaO–P2O5 glass in simulated body fluid, J. Non. Cryst. Solids. 356 (2010) 1470–1478.
 R. Kamalian, A. Yazdanpanah, F. Moztarzadeh, R. Ravarian, Z. Moztarzadeh, M. Tahmasbi, # Masoud Mozafari, Synthesis And Characterization Of Bioactive Glass/Forsterite Nanocomposites For Bone Implants, Ceram. – Silikáty. 56 (2012) 331–340.
 V. K. Vyas, A. S. Kumar, S. Prasad, S. P. Singh, R. Pyare, Bioactivity and mechanical behaviour of cobalt oxide-doped bioactive glass, Bull. Mater. Sci. 38 (2015) 957–964.
 K. Zhang, H. Yan, D.C. Bell, A. Stein, L. F. Francis, Effects of materials parameters on mineralization and degradation of sol-gel bioactive glasses with 3D-ordered macroporous structures, J. Biomed. Mater. Res. 66A (2003) 860–869.
 L. L. Hench, J. Wilson, An Introduction to Bioceramics, World Scientific, 1993.
 J. R. Jones, New trends in bioactive scaffolds: The importance of nanostructure, J. Eur. Ceram. Soc. 29 (2009) 1275–1281.
 X. Chen, J. Ou, Y. Wei, Z. Huang, Y. Kang, G. Yin, Effect of MgO contents on the mechanical properties and biological performances of bioceramics in the MgO–CaO–SiO2 system, J. Mater. Sci. Mater. Med. 21 (2010) 1463–1471.
 D. Khvostenko, T. J. Hilton, J. L. Ferracane, J. C. Mitchell, J. J. Kruzic, Bioactive glass fillers reduce bacterial penetration into marginal gaps for composite restorations, Dent. Mater. 32 (2016) 73–81.
 J. P. Ruparelia, A. K. Chatterjee, S. P. Duttagupta, S. Mukherji, Strain specificity in antimicrobial activity of silver and copper nanoparticles, Acta Biomater. 4 (2008) 707–716.
 Robinson D Griffith R Shechtman D Evans R Conzemius M, In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, Acta Biomater. 6 (2010) 1869–1877.
 I. Allan, H. Newman, M. Wilson, Antibacterial activity of particulate Bioglass® against supra- and subgingival bacteria, Biomaterials. 22 (2001) 1683–1687.
 M. Kazem-Rostami, A. Moghanian, Hünlich base derivatives as photo-responsive Λ-shaped hinges, Org. Chem. Front. 4 (2017) 224–228.
 A. Khazaei, S. Saednia, J. Saien, M. Kazem-Rostami, M. Sadeghpour, M. K. Borazjani, F. Abbasi, Grafting Amino Drugs to Poly(styrene- alt -maleic Anhydride) as a Potential Method for Drug Release, J. Braz. Chem. Soc. 24 (2013) 1109–1115.