Commenced in January 2007
Paper Count: 30745
Comparative Forensic Analysis of Lipsticks Using Thin Layer Chromatography and Gas Chromatography
Abstract:Lipsticks constitute a significant source of transfer evidence, and can, therefore, provide corroborative or inclusionary evidence in criminal investigation. This study aimed to determine the uniqueness and persistence of different lipstick smears using Thin Layer Chromatography (TLC), and Gas Chromatography with a Flame Ionisation Detector (GC-FID). In this study, we analysed lipstick smears retrieved from tea cups exposed to the environment for up to four weeks. The n-alkane content of each sample was determined using GC-FID, while TLC was used to determine the number of bands, and retention factor of each band per smear. This study shows that TLC gives more consistent results over a 4-week period than GC-FID. It also proposes a maximum exposure time of two weeks for the analysis of lipsticks left in the open using GC-FID. Finally, we conclude that neither TLC nor GC-FID can distinguish lipstick evidence recovered from hypothetical crime scenes.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.3298918Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 574
 Borowska S, Brzoska MM. Metals in cosmetics: implications for human health. J. Appl. Toxicol., 2015, vol. 35, no. 6, pp. 551-572.
 Tahan GI, Santos NKS, Albuquerque AC, Martins I. Determination of parabens in serum by Liquid chromaatography-Tandem Mass Spectrometry: Correlation with lipstick use. Reg. Toxicol. Pharmacol., 2016, vol. 79, pp. 42-48.
 Da Silva E, David AM, Pejovic-Milic A. The quantification of total lead in lipstick specimens by total reflection X-ray fluorescence spectrometry. X-ray Spectrosc., 2015, vol. 44, pp. 451-457.
 Zakaria A, Ho YB. Heavy metals contamination in lipsticks and their associated health risks to lipstick consumers. Reg. Toxicol. Pharmacol., 2015, vol. 73, no. 1, pp. 191-195.
 Castello A, Alvarez-Segui M, Verdu F. Luminuous lip-prints as criminal evidence. Forensic Sci. Int., 2005, vol. 155, no. 2-3, pp. 185-187.
 Salahioglu F, Went MJ, Gibson SJ. Application of Raman spectroscopy for the differentiation of lipstick traces. Anal. Methods, 2013, vol. 5, no. 20, pp. 5392-5401.
 Barbaro A, Cormaci P, Barbaro A. DNA typing from lipstick prints left on the skin. Forensic Sci. Int: Genet., 2009, vol. 2, no. 1, pp. 125-126.
 Adamowicz MS, Labonte RD, Schienman JE. The potential of cosmetic applicators as a source of DNA for forensic analysis. J. Forensic Sci., 2015, vol. 60, no. 4, pp. 1001-1011.
 Webb LG, Egan SE, Turbett GR. Recovery of DNA for Forensic Analysis from Lip Cosmetics. J Forensic Sci 2001; 46: 1474-9.
 Joshi B, Verma, K, and Singh J. A Comparison of Red Pigments in Different Lipsticks Using Thin Layer Chromatography (TLC). J. Anal. Bioanal. Techn., 2013, vol. 4, no. 1, pp. 157-161.
 Jasuja OP, Singh R. Thin-layer chromatographic analysis of liquid lipsticks. J. Forensic Identif., 2005, vol. 55, no. 1, pp. 28-35.
 Andrasko, J. Forensic Analysis of Lipsticks. Forensic Sci. Int., 1981, vol. 17, pp. 235-251.
 Reuland DJ, Trinler WA. A comparison of lipstick smears by High Performance Liquid Chromatography. Part II. The effects of wear-time and subject on the chromatograms. J. Forensic Sci. Soc., 1984, vol. 24, no. 5, pp. 509-518.
 Sharma V, Bharti A, Kumar R. On the spectroscopic investigation of lipstick stains: Forensic trace evidence. Spectrochim Acta Part A: Mol. and Biomol. Spectros., 2019, vol. 215, pp. 48-57.
 Wong JXW, Sauzier G, Lewis SW. Forensic determination of lipsticks using visible and attenuated total reflectance infrared spectroscopy. Forensic Sci. Int., 2019, vol. 298, pp. 88-96.
 Ehara, Y. and Marumo, Y. Identification of lipstick smears by fluorescence observation and purge-and-trap gas chromatography. Forensic Sci. Int., 1998, vol. 96, no. 1, pp. 1–10.
 Wang SW, Hsu KH, Huang SC, Tseng SS, Wang DY, Cheng HF. Determination of polycyclic aromatic hydrocarbons (PAHs) in cosmetic products by gas chromatography-tandem mass spectrometry J. Food Drug Anal., 2019, Submitted for publication.
 Choudhry, M. Y. Comparison of minute smears of lipstick by microspectrophotometry and scanning electron-microscopy energy dispersive spectroscopy. J. Forensic Sci., 1991, vol. 36, no. 2, pp. 366-375.
 Salahioglu F, Went MJ. Differentiation of lipsticks by Raman spectroscopy. Forensic Sci. Int., 2012, vol. 223, no. 1-3, pp. 148-152.
 Joshi B, Verma K, Singh J. J. Anal. Bioanal Tech., 2013, vol. 4, no. 1, pp. 1-4.
 Abdullah AFL, Marimuthu Y, Haw CK, Said NM, Muslim NZM, Hassan NFK, Yacoob MH, Hooi YC. Forensic discrimination of lipsticks by thin layer chromatography and gas chromatography-mass spectrometry. Malaysian J. Forensic Sci., vol. 2, no. 1, 2011, pp. 22-28.