The Effects of Drought and Nitrogen on Soybean (Glycine max (L.) Merrill) Physiology and Yield

Authors: Oqba Basal, András Szabó

Abstract:

Legume crops are able to fix atmospheric nitrogen by the symbiotic relation with specific bacteria, which allows the use of the mineral nitrogen-fertilizer to be reduced, or even excluded, resulting in more profit for the farmers and less pollution for the environment. Soybean (Glycine max (L.) Merrill) is one of the most important legumes with its high content of both protein and oil. However, it is recommended to combine the two nitrogen sources under stress conditions in order to overcome its negative effects. Drought stress is one of the most important abiotic stresses that increasingly limits soybean yields. A precise rate of mineral nitrogen under drought conditions is not confirmed, as it depends on many factors; soybean yield-potential and soil-nitrogen content to name a few. An experiment was conducted during 2017 growing season in Debrecen, Hungary to investigate the effects of nitrogen source on the physiology and the yield of the soybean cultivar 'Boglár'. Three N-fertilizer rates including no N-fertilizer (0 N), 35 kg ha^-1 of N-fertilizer (35 N) and 105 kg ha^-1 of N-fertilizer (105 N) were applied under three different irrigation regimes; severe drought stress (SD), moderate drought stress (MD) and control with no drought stress (ND). Half of the seeds in each treatment were pre-inoculated with Bradyrhizobium japonicum inoculant. The overall results showed significant differences associated with fertilization and irrigation, but not with inoculation. Increasing N rate was mostly accompanied with increased chlorophyll content and leaf area index, whereas it positively affected the plant height only when the drought was waived off. Plant height was the lowest under severe drought, regardless of inoculation and N-fertilizer application and rate. Inoculation increased the yield when there was no drought, and a low rate of N-fertilizer increased the yield furthermore; however, the high rate of N-fertilizer decreased the yield to a level even less than the inoculated control. On the other hand, the yield of non-inoculated plants increased as the N-fertilizer rate increased. Under drought conditions, adding N-fertilizer increased the yield of the non-inoculated plants compared to their inoculated counterparts; moreover, the high rate of N-fertilizer resulted in the best yield. Regardless of inoculation, the mean yield of the three fertilization rates was better when the water amount increased. It was concluded that applying N-fertilizer to provide the nitrogen needed by soybean plants, with the absence of N₂-fixation process, is very important. Moreover, adding relatively high rate of N-fertilizer is very important under severe drought stress to alleviate the drought negative effects. Further research to recommend the best N-fertilizer rate to inoculated soybean under drought stress conditions should be executed.

Keywords: Drought stress, inoculation, N-fertilizer, soybean physiology, yield.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1474431

References:

[1] J. He, Y-L. Du, T. Wang, N. C. Turner, R-P. Yang, Y. Jin, Y. Xi, C. Zhang, T. Cui,X-W. Fang and F-M. Li, “Conserved water use improves the yield performance of soybean (Glycine max (L.) Merr.) under drought,” Agricultural Water Management, vol. 179, pp. 236-245, 2016.
[2] D. Li, H. Liu, Y. Qiao, Y. Wang, Z. Cai, B. Dong, Ch. Shi, Y. Liu, X. Li and M. Liu, “Effects of elevated CO2on the growth, seed yield, and water use efficiency of soybean (Glycine max (L.) Merr.) under drought stress,” Agricultural Water Management, vol. 129, pp. 105–112, 2013.
[3] L. Hao, Y. Wang, J. Zhang, Y. Xie, M. Zhang, L. Duan and Z. Li, “Coronatine enhances drought tolerance via improving antioxidative capacity to maintaining higher photosynthetic performance in soybean,” Plant Science, vol. 210, pp. 1–9, 2013.
[4] P. Cerezini, B. H. Kuwano, M. B. dos Santos, F. Terassi, M. Hungria and M. A. Nogueira, “Strategies to promote early nodulation in soybean under drought,” Field Crops Research, vol. 196, pp. 160–167, 2016.
[5] X-D. Fan, J-Q. Wang, N. Yang, Y-Y. Dong, L. Liu, F-W. Wang, N. Wang, H. Chen, W-C. Liu, Y-P. Sun, J-Y. Wu and H-Y. Li, “Gene expression profiling of soybean leaves and roots under salt, saline–alkali and drought stress by high-throughput Illumina sequencing,” Gene, vol. 512, pp. 392–402, 2013.
[6] F. Liu, Ch. R. Jensen and M. N. Andersen, “Drought stress effect on carbohydrate concentration in soybean leaves and pods during early reproductive development: its implication in altering pod set,” Field Crops Research, vol. 86, pp. 1–13, 2004.
[7] M. Sincik, B. Candogan, C. Demirtas, H. Büyükcangaz, S. Yazgan and A. Göksoy, “Deficit irrigation of soya bean (Glycine max (L.) Merr.) in a sub-humid climate,”. Journal of Agronomy and Crop Science, vol. 194, pp. 200–205, 2008.
[8] T. R. Sinclair, L. C. Purcell, C. A. King, C. H. Sneller, P. Chen and V. Vadez, “Drought tolerance and yield increase of soybean resulting from improved symbiotic N2 fixation,” Field Crops Research, vol. 101, pp. 68–71, 2007.
[9] L. P. Manavalan, S. K. Guttikonda, L. S. P. Tran and H. T. Nguyen, “Physiological and molecular approaches to improve drought resistance in soybean,” Plant Cell Physiol., vol. 50, pp. 1260–1276, 2009.
[10] M. Reynolds and R. Tuberosa, “Translational research impacting on crop productivity in drought-prone environments,” Curr. Opin. Plant Biol., vol. 11 no. 2, pp. 171-179, 2008.
[11] J. Imsande, “Agronomic characteristics that identify high yield, high protein soybean genotypes,” Agron. J., vol. 84, pp. 409–414, 1992.
[12] J. R. Frederick, J. T. Woolley, J. D. Hesketh and D. B. Peters, “Phenological Responses of Old and Modern Soybean Cultivars to Air Temperature and Soil Moisture Treatment,” Field Crops Research, vol. 21, pp. 9-18, 1989.
[13] J. Dong, X. Xiao, P. Wagle, G. Zhang, Y. Zhou, C. Jin, M. S. Torn, T. P. Meyers, A. E. Suyker, J. Wang, H. Yan, Ch. Biradar and B. Moore, “Comparison of four EVI-based models for estimating gross primary production of maize and soybean croplands and tallgrass prairie under severe drought,” Remote Sensing of Environment, vol. 162, pp. 154–168, 2015.
[14] F. A. Kadhem, J. E. Specht and J. H. Williams, “Soybean irrigation serially timed during stages R1 to R6. I. Agronomic responses,” Agron. J., vol. 77, pp. 291-298, 1985.
[15] R. C. Muchow, “Canopy development in grain legumes grown under different soil water regimes in a semi-arid tropical environment,” Field Crops Research, vol. 11, pp. 99-109, 1985.
[16] S. Atti, R. Bonnell, D. Smith and S. Prasher, “Response of an Indeterminate Soybean {Glycine max (L.) Merr} to Chronic Water Deficit During Reproductive Development Under Greenhouse Conditions,” Canadian Water Resources Journal / Revue canadienne des ressources hydriques, vol. 29, no. 4, pp. 209-222, 2004.
[17] M. Mak, M. Babla, S. C. Xu, A. O’Carrigan, X. H. Liu, Y. M. Gong, P. Holford and Z. H. Chen, “Leaf mesophyll K+, H+ and Ca2+ fluxes are involved in drought-induced decrease in photosynthesis and stomatal closure in soybean,”. Environmental and Experimental Botany vol. 98, pp. 1– 12, 2014.
[18] J. L. Monteith and R. K. Scott, “Weather and yield variation of crops,”. In: K. Blaxter and L. Fowden (Editors), Food, Nutrition and Climate. Applied Science Publishers, Barking, Great Britain, pp. 127-149, 1982.
[19] T. R. Sinclair, S. C. Spaeth and J. S. Vendeland, “Microclimate limitations to crop yield,”. In: M.H. Miller, D.M. Brown, E.G. Beauchamp (Eds.), Breaking the Soil/Climate Barriers to Crop Yield, University of Guelph, Ontario, Canada (1981), pp. 3-27, 1981.
[20] X. Liu, J. Jin, G. Wang and S. J. Herbert, “Soybean yield physiology and development of high-yielding practices in Northeast China,” Field Crops Research, vol. 105, pp. 157–171, 2008.
[21] S. Bajaj, P. Chen, D. E. Longer, A. Shi, A. Hou, T. Ishibashi and K. R. Brye, “Irrigation and planting date effects on seed yield and agronomic traits of early-maturing Soybean,” J. Crop Improv., vol. 22, no. 1, pp. 47–65, 2008.
[22] E. Dogan, H. Kirnak and O. Copur, “Deficit irrigations during soybean reproductive stages and CROPGRO-soybean simulations under semi-arid climatic conditions,” Field Crops Research, vol. 103, no. 2, pp. 154–159, 2007.
[23] S. Gercek, E. Boydak, M. Okant and M. Dikilitas, “Water pillow irrigation compared to furrow irrigation for soybean production in a semi-arid area,” Agricultural Water Management, vol. 96, no. 1, pp. 87–92, 2009.
[24] N. Sionit and P. J. Kramer, “Effect of water stress during different stages of growth of soybeans,” Agronomy Journal, vol. 69, pp. 274-278, 1977.
[25] A. Maleki, A. Naderi, R. Naseri, A. Fathi, S. Bahamin and R. Maleki, “Physiological Performance of Soybean Cultivars under Drought Stress,” Bull. Env. Pharmacol. Life Sci., vol. 2, no. 6, pp. 38-44, 2013.
[26] Y. Ishibashi, H. Yamaguchi, T. Yuasa, M. Iwaya-Inoue, S. Arima and S. Zheng, “Hydrogen peroxide spraying alleviates drought stress in soybean plants,” Journal of Plant Physiology, vol. 168, pp. 1562–1567, 2011.
[27] W. Cui, Z. Chang and N. Li, “Effect of drought stress on physiology ecology and yield of soybean,” Journal of Water Resources and Water Engineering, vol. 24, pp. 20–24. (in Chinese), 2013.
[28] F. Fabre and C. Planchon, “Nitrogen nutrition, yield and protein content in soybean,” Plant Science, vol. 152, pp. 51–58, 2000.
[29] N. Fageria and V. Baligar, “Enhancing nitrogen use efficiency in crop plants,” Adv. Agron., vol. 88, pp. 97–185, 2005.
[30] F. Salvagiotti, K. G. Cassman, J. E. Specht, D. T. Walters, A. Weiss and A. Dobermann, “Nitrogen uptake, fixation and response to fertilizer N in soybeans: A review,” Field Crops Research, vol. 108, pp. 1–13, 2008.
[31] G. Yinbo, M. B. Peoples and B. Rerkasem, “The effect of N fertilizer strategy on N 2 fixation, growth and yield of vegetable soybean,” Field Crops Research, vol. 51, pp. 221-229, 1997.
[32] B. J. Ferguson, A. Indrasumunar, S. Hayashi, M-H. Lin, Y-H. Lin, D. E. Reid and P. M. Gresshoff, “Molecular analysis of legume nodule development and autoregulation,” J. Integr. Plant Biol., vol. 52, pp. 61–76, 2010.
[33] G. Seneviratne, L. H. J. Holm and E. M. H. G. Ekanayake, “Agronomic benefits of rhizobial inoculant use over nitrogen fertilizer application in tropical soybean,” Field Crops Research, vol. 68, pp. 199-203, 2000.
[34] J. Ying, D. F. Herridge, M. B. Peoples and B. Rerkasem, “Effect of N fertilization on N, fixation and N balances of soybean grown after lowland rice,” Plant and Soil, vol. 147, pp. 235-242, 1992.
[35] M. Hungria, J. Franchini, R. Campo and P. Graham, “The importance of nitrogen fixation to soybean cropping in South America,” In: Werner, D., Newton, W. (Eds.), Nitrogen Fixation in Agriculture, Forestry, Ecology, and the Environment. Springer, Netherlands, pp. 25–42, 2005.
[36] S. Caliskan, I. Ozkaya, M. E. Caliskan and M. Arslan, “The effects of nitrogen and iron fertilization on growth, yield and fertilizer use efficiency of soybean in a Mediterranean-type soil,” Field Crops Research, vol. 108, pp. 126–132, 2008.
[37] J. E. Harper, “Soil and symbiotic nitrogen requirements for optimum soybean production,” Crop Sci., vol. 14, pp. 255–260, 1974.
[38] M. Obaton, M. Miquel, P. Robin, G. Conejero, A. Domenach and R. Bardin, “Influence du deficit hydrique sur l'activite nitrate reductase et nitrogenase chez le Soja (Glycine max L. Merr. cv. Hodgson),” C.R. Acad. Sci. Paris, vol. 294, pp. 1007-1012, 1982.
[39] L. C. Purcell and C. A. King, “Drought and Nitrogen Source Effects on Nitrogen Nutrition, Seed Growth and Yield in Soybean,” J. Plant Nutr., vol. 19, pp. 969-993, 1996.
[40] W. R. Fehr and C. E. Caviness, “Stages of soybean development,” Special Report. 87. http://lib.dr.iastate.edu/specialreports/87, 1977.
[41] C. J. DeMooy, J. Pesek and E. Spaldon, “Mineral nutrition of soybeans. In: ed. B.E. Caldwell, Soybeans: Improvement, Production, and Uses,” Agronomy Series. ASA Publishers, Madison, pp. 276-352, 1973.
[42] I. Watanabe, K. Tabuchi and H. Nakano, “Response of soybean to supplemental nitrogen after flowering,” In: ed. S. Shanmugasundaram, E.W. Sulzberger and B.T. Mclean, Soybean in Tropical and Subtropical Cropping Systems. AVRDC, Shanhua, Taiwan, pp. 301-308, 1986.
[43] T. Sinclair and R. Serraj, “Legume Nitrogen-Fixation and Drought,” Nature, vol. 378, p. 344, 1995.
[44] J. Levitt, “Responses of plants to environmental stresses,” Academic Press. New York and London. 697 pp, 1980.
[45] R. B. Austin, “Maximizing crop production in water limited environments,” 13-25. In: F. W. G. Baker. Drought resistance in cereals. CAB International, Wallingford, England 2220, 1989.
[46] E. Rurangwa, B. Vanlauwe and K. E. Giller, “Benefits of inoculation, P fertilizer and manure on yields of common bean and soybean also increase yield of subsequent maize,” Agriculture, Ecosystems and Environment, vol. 261, pp. 219-229, 2018.
[47] M. Albareda, D. N. Rodriguez-Navarro and F. J. Temprano, “Soybean inoculation: Dose, N fertilizer supplementation and rhizobia persistence in soil,” Field Crops Research, vol. 113, pp. 352–356, 2009.
[48] S. Zimmer, M. Messmer, Th. Haase, H-P. Piepho, A. Mindermann, H. Schulz, A. Habekuß, F. Ordon, K-P. Wilbois and J. Heß, “Effects of soybean variety and Bradyrhizobium strains on yield, protein content and biological nitrogen fixation under cool growing conditions in Germany,” Europ. J. Agronomy, vol. 72, pp. 38–46, 2016.
[49] G. Kaschuk, M. A. Nogueira, M. J. de Luca and M. Hungria, “Response of determinate and indeterminate soybean cultivars to basal and topdressing N fertilization compared to sole inoculation with Bradyrhizobium,” Field Crop Research, vol. 195, pp. 21–27, 2016.
[50] A. F. MacKenzie and P. C. Kirby, “Effects of fertilizers and soil series on yields of corn, barley, wheat and soybeans,” Soil Fertility Research at Macdonald College: a summary of 1968-1978. McGill University, Montreal, PQ, 1979.
[51] D. L. Dornbos and R. E. Mullen, “Soybean seed protein and oil contents and fatty acid composition adjustments by drought and temperature,” Journal of the American Oil Chemists' Society, vol. 69, no. 3, pp. 228-231, 1992.