Effects of Pressure and Temperature on the Extraction of Benzyl Isothiocyanate by Supercritical Fluids from Tropaeolum majus L. Leaves
Tropaeolum majus L. is a native plant to South and Central America, used since ancient times by our ancestors to combat different diseases. Glucotropaeolonin is one of its main components, which when hydrolyzed, forms benzyl isothiocyanate (BIT) that promotes cellular apoptosis (programmed cell death in cancer cells). Therefore, the present research aims to evaluate the effect of the pressure and temperature of BIT extraction by supercritical CO2 from Tropaeolum majus L. The extraction was carried out in a supercritical fluid extractor equipment Speed SFE BASIC Brand: Poly science, the leaves of Tropaeolum majus L. were ground for one hour and lyophilized until obtaining a humidity of 6%. The extraction with supercritical CO2 was carried out with pressures of 200 bar and 300 bar, temperatures of 50°C, 60°C and 70°C, obtained by the conjugation of these six treatments. BIT was identified by thin layer chromatography using 98% BIT as the standard, and as the mobile phase hexane: dichloromethane (4:2). Subsequently, BIT quantification was performed by high performance liquid chromatography (HPLC). The highest yield of oleoresin by supercritical CO2 extraction was obtained pressure 300 bar and temperature at 60°C; and the higher content of BIT at pressure 200 bar and 70°C for 30 minutes to obtain 113.615 ± 0.03 mg BIT/100 g dry matter was obtained.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1316534Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 513
 Nanzi, A. Monograph CAPUCHINA. 1999. Retrieved on December 12, 2016 ofwww.youblisher.com/p/61602-Capuchina-Tropaeolum-majus-L/
 Colbert “New Bible Cure for Cancer: ancient truths and natural remedies and the latest findings for health” 2010.
 Spiridon, E. & Barberaki, M. “Plants Fight Cancer That”. Editorial Taylor Francis Group. London and New York. 2003. Page 187.
 Lykkesfeld, J. & Lindberg MB “Synthesis of Benzylglucosinolate Tropaeolum majus L. in Plant Biochemistry Laboratory”. Retrieved on December 12, 2016 of maltawildplants.com/TROP/Docs/TPLMJ/TRPMJ-Synthesis_Benzylglucosinolate.pdf.
 Benedí G., C. and P. Simon, J. “Toxiques Plantes ornamentals” Barcelona (2013).
 Barroso, P., de Carvalho, P., Rochab, T., Pessoa, F., Azevedo, D., & Mendes, M. Evaluation of the composition of Carica papaya L. seed oil supercritical CO2 Extracted With. Biotechnology Reports Volume 11, September 2016, Pages 110-116.
 Rodriguez, TL Determination of in vitro antibacterial activity of benzyl isothiocyanate carica pubescens (Mount papaya) against Helicobacter pylori. Basadrina Medical Journal. Basadrina Medical Journal. UNJBG - FACS. 2011.
 USP. The United States Pharmacopeial Convention. 2007. 39. usp United States of America. Pag 499-500.
 Ahluwalia, S., Shivhare, U. & Basu, S. Supercritical CO2 Extraction of Compounds With Activity Antioxidant Fruits and Vegetables from Waste -A Review. Focusing on Modern Food Industry (FMFI) Volume 2 Issue 1 February 2013.