Effect of Three Drying Methods on Antioxidant Efficiency and Vitamin C Content of Moringa oleifera Leaf Extract
Moringa oleifera is a plant containing many nutrients that are mostly concentrated within the leaves. Commonly, the separation process of these nutrients involves solid-liquid extraction followed by evaporation and drying to obtain a concentrated extract, which is rich in proteins, vitamins, carbohydrates, and other essential nutrients that can be used in the food industry. In this work, three drying methods were used, which involved very different temperature and pressure conditions, to evaluate the effect of each method on the vitamin C content and the antioxidant efficiency of the extracts. Solid-liquid extractions of Moringa leaf (LE) were carried out by employing an ethanol solution (35% v/v) at 50 °C for 2 hours. The resulting extracts were then dried i) in a convective oven (CO) at 100 °C and at an atmospheric pressure of 750 mbar for 8 hours, ii) in a vacuum evaporator (VE) at 50 °C and at 300 mbar for 2 hours, and iii) in a freeze-drier (FD) at -40 °C and at 0.050 mbar for 36 hours. The antioxidant capacity (EC50, mg solids/g DPPH) of the dry solids was calculated by the free radical inhibition method employing DPPH˙ at 517 nm, resulting in a value of 2902.5 ± 14.8 for LE, 3433.1 ± 85.2 for FD, 3980.1 ± 37.2 for VE, and 8123.5 ± 263.3 for CO. The calculated antioxidant efficiency (AE, g DPPH/(mg solids·min)) was 2.920 × 10-5 for LE, 2.884 × 10-5 for FD, 2.512 × 10-5 for VE, and 1.009 × 10-5 for CO. Further, the content of vitamin C (mg/L) determined by HPLC was 59.0 ± 0.3 for LE, 49.7 ± 0.6 for FD, 45.0 ± 0.4 for VE, and 23.6 ± 0.7 for CO. The results indicate that the convective drying preserves vitamin C and antioxidant efficiency to 40% and 34% of the initial value, respectively, while vacuum drying to 76% and 86%, and freeze-drying to 84% and 98%, respectively.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1314542Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 922
 L. Gopalakrishnan, K. Doriya and D. S. Kumar. “Moringa oleifera: A review on nutritive importance and its medicinal application,” Food Sci. Human Wellness, vol. 5, pp. 49-56, 2016.
 N. Sankhyan, A. Sharma, C. A. Seth, A. Chauhan and S. Kulshrestha, “Determination and comparison of vitamin C content from Moringa oleifera by different methods,” Int. J. Agric. Sci. Res., vol. 3, pp. 67-70, 2013.
 G. Valdés-Hernández, L. Cruz-Viera and R. Comet-Rodríguez. “Influencia de las condiciones de operación en la extracción de polifenoles a partir de hojas de Moringa oleifera Lam,” Revista CENIC. Ciencias Químicas, vol.46, pp. 135-145, 2015.
 C. Sánchez-Moreno, J. Larrauri and F. Saura-Calixto. “A Procedure to Measure the Antiradical Efficiency of Polyphenols,” J. Sci. Food Agric., vol. 76 , pp. 270–276, 1998.
 P. H. M. Marfil, E. M. Santos and V. R. N. Telis. “Ascorbic acid degradation kinetics in tomatoes at different drying conditions,” Food Sci. Technol., vol. 4, pp. 1642-1647, 2008.