Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 31023
Effect of Bacillus subtilis Pb6 on Growth and Gut Microflora in Clostridium perfringens Challenged Broilers

Authors: A. Khalique, T. Naseem, N. Haque, Z. Rasool


The objective of current study was to investigate the effect of Bacillus subtilis PB6 (CloSTAT) as a probiotic in broilers. The corn-soybean based diet was divided into four treatment groups; T1 (basal diet with no probiotic and no Clostridium perfringens); T2 (basal diet challenged with C. perfringens without probiotic); T3 (basal diet challenged with C. perfringens having 0.05% probiotic); T4 (basal diet challenged with C. perfringens having 0.1% probiotic). Every treatment group had four replicates with 24 birds each. Body weight and feed intake were measured on weekly basis, while ileal bacterial count was recorded on day-28 following Clostridium perfringens challenge. The 0.1% probiotic treatment showed 7.2% increase in average feed intake (P=0.05) and 8% increase in body weight compared to T2. In 0.1% treatment body weight was 5% higher than T3 (P=0.02). It was also observed that 0.1% treatment had improved feed conversion ratio (1.77) on 6th week. No effect of treatment was observed on mortality and ileal bacterial count. The current study indicated that 0.1% use of probiotic had positive response in C. perfringens challenged broilers.

Keywords: broilers, clostridium perfringens, Bacillus subtilis PB6, antibiotic growth promoters, CloSTAT

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 687


[1] T. Netherwood, H. J. Gilbert, D. S. Parker, and A. G. O’Donnell, “Probiotics shown to change bacterial community structure in the avian gastrointestinal tract”, Appl. Environ. Microbiol. vol. 65, Nov. 1999, pp. 5134–5138.
[2] F. Van Immerseel, J. De Buck, F. Pasmans, G. Huyghebaert, F. Haesebrouck, and R. Ducatelle, “Clostridium perfringensin poultry: an emerging threat for animal and public health”, Avian Pathol., vol. 33, Dec. 2004, pp. 537-549.
[3] J. Patterson, and K. Burkholder, 2003. “Application of prebiotics and probiotics in poultry production”, Poult. Sci., vol. 82, Apr. 2003, pp. 627-631.
[4] R. W. Jack, J. R. Tagg, and B. Ray, “Bacteriocins of gram positive bacteria. Microbiol. Rev., vol. 59, Jun. 1995, pp. 171-200.
[5] A. L. Teo, and H. M. Tan, 2006. “Effect of Bacillus subtilis PB6 (CloSTAT) on broilers infected with a pathogenic strain of Escherichia coli”, J. Appl. Poult. Res., vol. 15, Summer 2006, pp.229-235.
[6] M. R. Spinosa, T. Braccini, E. Ricca, M. De Felice, L. Morelli, G. Pozzi, and M. R. Oggioni, “On the fate of ingested Bacillus spores”, Res. Microbiol., Vol. 151, Jun. 2000, pp.361– 368.
[7] A. L. Teo, and H. M. Tan, “Inhibition of Clostridium perfringens by a novel strain of Bacillus subtilis isolated from the gastrointestinal tracts of healthy chickens”, Appl. Environ. Microbiol., vol. 71, Aug. 2005, pp. 4185–4190.
[8] A. Y. Teo, and H.M. Tan, “Evaluation of the performance and intestinal gut microflora of broilers fed on corn-soy diets supplemented with Bacillus subtilis PB6 (CloSTAT)”, J. Appl. Poult. Res., vol. 16, Summer 2007, pp. 296-303.
[9] A. M. Abudabos, A. H. Alyemni, and M. B. A. Al Marshad, “Bacillus subtilis PB6 based-probiotic (CloSTATTM) improves intestinal morphology and microbiological status of broiler chickens under Clostridium perfringens challenge”, Int. J. Agric. Biol., vol. 15, Mar, 2013, pp. 978–982.
[10] S. Jayaraman, G. Thangavel, H. Kurian, R. Mani, R. Mukkalil, and H. Chirakkal, “Bacillus subtilis PB6 improves intestinal health of broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis”, Poult. Sci., vol. 92, Feb 2013, pp.370-374.
[11] A. R. Gholamiandehkordi, L. Timbermont, A. Lanckriet, W.V.D. Broeck, K.Pedersen, J. Dewulf, F. Pasmans, J.L. Grimes, S. Rahimi, E. Oviedo,B.W. Sheldon, and F.B.O. Santos, “Effects of a direct-fed microbial (Primalac) on turkey poult performance and susceptibility to oral salmonella challenge” Poult. Sci., vol. 87, Jul. 2008, pp. 1464–1470.
[12] S. Sen,S. Ingale, Y. Kim, J. Kim, K. Kim, J. Lohakare , E. H. Kim E, Kim , M. Ryu , and I. Kwon I, “Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology”, Res.Vet. Sci., vol. 93, Aug. 2012, pp. 264-268.
[13] B. Mohan, R. Kadirvel, A. Natarajan, and M. Bhaskaran, “Effect of probiotic supplementation on growth, nitrogen utilisation and serum cholesterol in broilers”, Br. Poult. Sci., vol. 37, May 1996, pp. 395-401.
[14] C. Fritts, J. Kersey, M. Motl, E. Kroger, F. Yan, J. Si, Q. Jiang, M. Campos, A. Waldrou, and P. Waldroup, “Bacillus subtilis C-3102 (Calsporin) improves live performance and microbiological status of broiler chickens”, J. Appl. Poult. Res., vol. 9, Summer 2000, pp. 149-155.
[15] T. Melegy, N. F. Khaled, R. El-Bana, and H. Abdellatif, “Effect of Dietary Supplementation Bacillus subtilis PB6(CLOSTATTM) on Performance, Immunity, Gut Health and Carcass Traits in Broilers”, J. Amer. Sci., vol. 7, Dec. 2001, pp. 891-898.
[16] A. Khaksefidi, and T. Ghoorchi, “Effect of probiotic on performance and immunocompetence in broiler chicks”, J. Poult. Sci., vol. 43, Aug. 2006, pp. 296-300.
[17] R. M. La Ragione, and M. J. Woodward, “Competitive exclusion by Bacillus subtilis spores of Salmonella entericaserotype Enteritidis and Clostridium perfringens in young chickens”. Vet. Microbiol. vol. 94, Jul. 2003, pp. 245-256.
[18] K. Maruta, H. Miyazak, S. Masuda, M. Takahashi, T. Marubashi, Y. Tadano, and H. Takahashi, “Exclusion of intestinal pathogens by continuous feeding with Bacillus subtilis C-3102, and its influence on the intestinal microflora in broilers”, Anim. Sci. Technol., vol. 67, Mar. 1996, pp. 273-280.