Evaluation of Thrombolytic Activity of Zingiber cassumunar Roxb. and Thai Herbal Prasaplai Formula
The propose of this study was to investigate in vitro thrombolytic activity of Zingiber cassumunar Roxb. and Prasaplai, a Thai herbal formulation of Z. cassumunar Roxb. Herbs were extracted with boiling water and concentrated by lyophilization. To observe their thrombolytic potential, an in vitro clot lysis method was applied where streptokinase and sterile distilled water were used as positive and negative controls, respectively. Crude aqueous extracts from Z. cassumunar Roxb. and Prasaplai formula showed significant thrombolytic activity by clot lysis of 17.90% and 25.21%, respectively, compared to the negative control water (5.16%) while the standard streptokinase revealed 64.78% clot lysis. These findings suggest that Z. cassumunar Roxb. exhibits moderate thrombolytic activity and cloud play an important role in the thrombolytic properties of Prasaplai formula. However, further study should be done to observe in vivo clot dissolving potential and to isolate active component(s) of these extracts.
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 J.C. Mucklow, “Thrombolytic treatment streptokinase is more economical than Alteplase,” Bitrish Medicine Journal, vol. 311, no. 7018, p. 1506, 1995.
 D. Collen, “Coronary thrombosis: streptokinase or recombinant tissue‐type plasminogen activator,” Annals of internal medicine, vol. 112, no. 7, pp. 529–538, 1990.
 D. B. Baruah, R. N. Dash, M. R. Chaudhari, and S. S. Kadam, “Plasminogen activators: A comparison,” Vascular pharmacology, vol. 44, no. 1, pp. 1–9, 2006.
 S. C. Patel, and A. Mody, “Cerebral hemorrhagic complications of thrombolytic therapy,” Progress in Cardiovascular Diseases, vol. 42, no. 3, pp. 217–233, 1999.
 S. A. Rouf, M. Moo‐Young, and Y. Chisti, “Tissue plasminogen activator: characteristics, applications and production technology,” Biotechnology Advances, vol. 4, no. 3, pp. 239–266, 1996.
 A. Das, S. M.R. Dewan, R. Ali, P.C. Debnath, and M. Billah, “Investigation of in vitro thrombolytic potential of ethanolic extract of Momordica charantia fruits: An anti-diabetic medicinal plant,” Der Pharmacia Sinica, vol. 4, no. 2, pp. 104-108, 2013.
 M. S. Kim, and K. A. Lee, “Antithrombotic activity of Methanolic Extract of Umbilicaria esculenta,” Journal of Ethnopharmacology, vol. 105, no. 3, pp. 342–345, 2006.
 Y. R. Jin, J. Y. Yu, J. J. Lee, S. H. You, J. H. Chung, J. Y. Noh, et al., “Antithrombotic and antiplatelet activities of Korean red ginseng extract,” Basic & Clinical Pharmacology & Toxicology, vol. 100, no. 3, pp. 170–175, 2007.
 J. J. Lee, T. Kim, W. K. Cho, and J. Y. Ma, “Antithrombotic and antiplatelet activities of Soshiho-tang extract” BMC Complementary and Alternative Medicine, vol. 13:137, 2013.
 T. Bordia, N. Mohammed, M.Thomson, and M. Ali, An evaluation of garlic and onion as antithrombotic agents,” Prostaglandins, Leukotrienes and Essential Fatty Acids, vol. 54, no. 3, pp. 183–186, 1996.
 H. S. Demrow, P. R. Slane, and J. D. Folts, “Administration of wine and grape juice inhibits in vivo platelet activity and thrombosis in stenosed canine coronaryartheries,” Circulation, vol. 91, no. 4, p. 1182, 1995.
 P. Tangyuenyongwatana, and W. Gritsanapan, “Standardization of Prasaplai, a Thai traditional preparation for antidysmenorrhea,” Botanics: Targets and Therapy, vol. 2016, no. 6, pp.1–9, 2015.
 S. Nualkaew, W, Gritanapan, F. Petereit, and A. Nahrstedt, “A new fatty acid esters originated during storage by the interaction of components in Prasaplai, a Traditional medicine,” Planta Medica, vol. 70, no. 12, pp. 1243–1246, 2004.
 C. Kamalashiran, and O. Lekskulchai, “Thecomparative study of the efficiency and side effect of Prasaplai extract versus mefenamic acid on relieving primary dysmenorrheal: a clinical phase II trials,” Thammasat Medical Journal, 12, no. 4, pp. 749–756,2012.
 C. C. Guzman, and S. S. Siemosma, Plant Resources of South-East Asia, spices no.13, Leiden, the Netherland, 1999.
 A. Kaewchoothong, S. Tewtrakul, and P. Panichayupakaranant, “Inhibitory effect of phenylbutanoid-rich Zingiber cassumunar extracts on nitric oxide production by murine macrophage-like RAW264.7 cells,” Phytotherapy Research, vol. 26, no. 12, pp. 1789–1792, 2012.
 R. Jeenapongsa, K. Yoovathaworn, K. M. Sriwatanakul, U. Pongprayoon, and K. Sriwatanakul, “Anti-inflammatory activity of (E)-1-(3,4-dimethoxyphenyl) butadiene from Zingiber cassumunar Roxb,” Journal of Ethnopharmacology, vol. 87, no.(2-3), pp. 143–148, 2003.
 A. Panthong, D.V. Kanjanapothi,. Niwatananun, P. Tuntiwachwuttikul, and V. Reutrakul, “Anti-inflammatory activity of compounds isolated from Zingiber cassumunar,” Planta Medica, vol, 56, no. 6p. 655, 1990.
 M. N. I. Bhuiyan, J. U. Chowdhury, and J. Begum, “Volatile constituents of essential oils isolated from leaf and rhizome of Zingiber cassumunar Roxb,” Bangladesh Journal of Pharmacology, vol. 3, pp. 69–73, 2008.
 Y. Ozaki, N. Kawahara, and M. Harada, “Anti-inflammatory effect of Zingiber cassumunar ROXB. and its active principles,” Chemical and Pharmaceutical Bulletin, vol. 39, no. 9, pp. 2353–2356, 1991.
 D. Kanjanapothi, P. Soparat, A. Panthong, P. Tuntiwachwuttikul, and V. Reutrakul, “A uterine relaxant compound from Zingiber cassumunar,” Planta Medica; vol. 53, no. 4, pp. 329–332,1987.
 N. R. Farnsworth and N. Bunyapraphatsara, Thai Medicinal Plants: Recommended for Primary Health Care System, Prachachon, Bangkok, Thailand, 1992.
 A. R. Han, M. S. Kim, Y. H. Jeong, S. K. Lee, and E. K. Seo, “Cyclooxygenase-2 inhibitory phenylbutenoids from the rhizomes of Zingiber cassumunar,” Chemical and Pharmaceutical Bulletin, vol. 53, no. 11, pp. 1466–1468, 2005.
 T. Masuda, and A. Jittoe, “Phenylbutenoid monomers from the rizhomes of Zigiber cassumunar,” Phytochemistry, vol39, no. 2, pp. 459–461, 1995.
 H. Dinter, R. Haensel, and A. Pelter, “The structures of cassumunaquinones 1 and 2 from Zingiber cassumunar Roxb,” Z Zeitschriftfür Naturforschung. C, vol. 35, pp. 154–155,1980.
 A. K. Bordoloi, J. Sperkova, and P. A. Leclercq, “Essential oils of Zingiber cassumunar roxb. From Northeast India,” Journal of Essential Oil Research, vol. 11, no. 4, pp. 441–445, 1999.
 A. Jitoe, T Masuda, and T. J. Mabry, “Novel Antioxidants, Cassumunarin A, B and C, for Zingiber cassumunar,” Tetrahedron Letters, vol. 35, no. 7, pp. 981–984,1994.
 T. Masuda, A. Jitoe, and T. J. Mabry, “Isolation and structure determination of cassumunarins A, B, and C: new anti-inflammatory antioxidants from a tropical ginger, Zingiber cassumunar,” Journal of the American Oil Chemists' Society, vol. 72, no. 9, pp. 1053–1057, 1995.
 T. Dechatiwongse and K. Yoshihira, “Chemical studies on the rhizome of plai (Zingiber cassumunar Roxb.),” Bulletin of the Department of Medical Sciences, vol. 15, no. 4, pp. 1–15,1973.
 T. Masuda, A. Jitoe, and N. Nakatani, “Structures of cassumunin a, b, and c, new potent antioxidants from Zingiber cassumunar Roxb,” Chemical Letters, vol. 22, no. 1, pp. 189–192, 1993.
 P. Tangyuenyongwatana, and W. Gritsanapan, “Prasaplai: An essential Thai traditional formulation for primary dysmenorrhea treatment,” TANG, vol. 4, no. 2, pp. 1–8.2014.
 N. V. Harbone, Phytochemical method A guide to modern techniques of plant analysis 2nd ed. Chapman and Hall London, 1994, p. 425.
 S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M Taori, and H. F. Daginawala, “Development of an in vitro model to study clot lysis activity of thrombolytic drugs,” Thrombosis Journal, vol. 4:4, 2006.
 S. Prasad, R. S. Kashyap, J. Y. Deopujari, H. J. Purohit, G. M. Taori, and H. F. Daginawala, “Effect of Fagoniaarabica (Dhamasa) on in vitro thrombolysis,” BMC Complementary and Alternative Medicine, vol. 7, :36, 2007.
 M. Ali, R. Akhter, S. N. Narjish, M. Shahriar and M. A. Bhuiyan, “Studies of preliminary phytochemical screening, membrane stabilizing activity, throbolytic activity and in vitro antioxidant activity of leafextract of Citrus hystrix,” International Journal of Pharmaceutical Sciences and Research, vol. 6, no.6, pp. 2367–2374, 2015.
 A. Bordia, S. K.Verma, and K. C. Srivastava, “Effect of garlic (Allium sativum) on blood lipids, blood sugar, fibrinogen and fibrinolytic activity in patients with coronary artery disease,” Prostaglandins, Leukotrienes and Essential Fatty Acids, vol. 58, no. 4, pp.257–263, 1998.
 S. K. Verma, and A. Bordia, “Ginger, fat and fibrinolysis,” Indian Journal of Medical Sciences, vol. 55, no. 2, pp. 83–86, 2001.