Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32578
Antibody-Conjugated Nontoxic Arginine-Doped Fe3O4 Nanoparticles for Magnetic Circulating Tumor Cells Separation

Authors: F. Kashanian, M. M. Masoudi, A. Akbari, A. Shamloo, M. R. Zand, S. S. Salehi


Nano-sized materials present new opportunities in biology and medicine and they are used as biomedical tools for investigation, separation of molecules and cells. To achieve more effective cancer therapy, it is essential to select cancer cells exactly. This research suggests that using the antibody-functionalized nontoxic Arginine-doped magnetic nanoparticles (A-MNPs), has been prosperous in detection, capture, and magnetic separation of circulating tumor cells (CTCs) in tumor tissue. In this study, A-MNPs were synthesized via a simple precipitation reaction and directly immobilized Ep-CAM EBA-1 antibodies over superparamagnetic A-MNPs for Mucin BCA-225 in breast cancer cell. The samples were characterized by vibrating sample magnetometer (VSM), FT-IR spectroscopy, Tunneling Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). These antibody-functionalized nontoxic A-MNPs were used to capture breast cancer cell. Through employing a strong permanent magnet, the magnetic separation was achieved within a few seconds. Antibody-Conjugated nontoxic Arginine-doped Fe3O4 nanoparticles have the potential for the future study to capture CTCs which are released from tumor tissue and for drug delivery, and these results demonstrate that the antibody-conjugated A-MNPs can be used in magnetic hyperthermia techniques for cancer treatment.

Keywords: Tumor tissue, antibody, magnetic nanoparticle, CTCs capturing.

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 993


[1] Sahoo, S. K., S. Parveen, and J. J. Panda. "The present and future of nanotechnology in human health care." Nanomedicine: Nanotechnology, Biology and Medicine 3.1 (2007): 20-31.
[2] Foldbjerg, Rasmus, Duy Anh Dang, and Herman Autrup. "Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549." Archives of toxicology 85.7 (2011): 743-750.
[3] Salgueiriño‐Maceira, Veronica, and Miguel A. Correa‐Duarte. "Increasing the complexity of magnetic core/shell structured nanocomposites for biological applications." Advanced Materials 19.23 (2007): 4131-4144.
[4] Latham, Andrew H., and Mary Elizabeth Williams. "Controlling transport and chemical functionality of magnetic nanoparticles." Accounts of chemical research 41.3 (2008): 411-420.
[5] Wang ZL. Nanobelts, nanowires, and nanodiskettes of semiconducting oxides—from materials to nanodevices. Advanced Materials. 2003 Mar 4;15(5):432-6.
[6] Olsvik O, Popovic T, Skjerve E, Cudjoe KS, Hornes E, Ugelstad J, Uhlen M. Magnetic separation techniques in diagnostic microbiology. Clinical microbiology reviews. 1994 Jan 1;7(1):43-54.
[7] Wang, Z., Zhu, H., Wang, X., Yang, F., & Yang, X. (2009). One-pot green synthesis of biocompatible arginine-stabilized magnetic nanoparticles. Nanotechnology, 20(46), 465606.
[8] Ebrahiminezhad, A., Ghasemi, Y., Rasoul-Amini, S., Barar, J., & Davaran, S. (2012). Impact of amino-acid coating on the synthesis and characteristics of iron-oxide nanoparticles (IONs). Bulletin of the Korean Chemical Society, 33(12), 3957-3962.
[9] Varadan, Vijay K., Linfeng Chen, and Jining Xie. Nanomedicine: design and applications of magnetic nanomaterials, nanosensors and nanosystems. John Wiley & Sons, 2008.
[10] Guimarães, Alberto P. Principles of nanomagnetism. Springer Science & Business Media, 2009.
[11] Jurgons, R., et al. "Drug loaded magnetic nanoparticles for cancer therapy." Journal of Physics: Condensed Matter 18.38 (2006): S2893.
[12] Vasir, Jaspreet K., and Vinod Labhasetwar. "Targeted drug delivery in cancer therapy." Technology in cancer research & treatment 4.4 (2005): 363-374.
[13] Corma, A., Fornes, V., Jorda, J. L., Rey, F., Fernandez-Lafuente, R., Guisan, J. M., & Mateo, C. (2001). Electrostatic and covalent immobilisation of enzymes on ITQ-6 delaminated zeolitic materials. Chemical Communications, (5), 419-420.