An Alternative Antimicrobial Approach to Fight Bacterial Pathogens from Phellinus linteus

Authors: S. Techaoei, K. Jarmkom, P. Eakwaropas, W. Khobjai

Abstract:

The objective of this research was focused on investigating in vitro antimicrobial activity of Phellinus linteus fruiting body extracts on Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. Phellinus linteus fruiting body was extracted with ethanol and ethyl acetate and was vaporized. The disc diffusion assay was used to assess antimicrobial activity against tested bacterial strains. Primary screening of chemical profile of crude extract was determined by using thin layer chromatography. The positive control and the negative control were used as erythromycin and dimethyl sulfoxide, respectively. Initial screening of Phellinus linteus crude extract with the disc diffusion assay demonstrated that only ethanol had greater antimicrobial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus. The MIC assay showed that the lower MIC was observed with 0.5 mg/ml of Pseudomonas aeruginosa and Methicillin-resistant Staphylococcus aureus and 0.25 mg/ml. of Escherichia coli and Staphylococcus aureus, respectively. TLC chemical profile of extract was represented at R_f ≈ 0.71-0.76.

Keywords: Staphylococcus aureus, Phellinus linteus, methicillin-resistant Staphylococcus aureus, antimicrobial activity, Escherichia coli.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1130059

References:

[1] A.S. Haddadin, S.A. Fappiano, and P.A. Lipsett, “Methicillin resistant Staphylococcus aureus (MRSA) in the intensive care unit” Postgrad. Med. J. 2002, Vol. 78, pp. 385-392.
[2] C.M. Kang, D.H. Han, H.K. Hwang, S.H. Choi, and W.J. Lee, “Anticancer effect of Phellinus linteus; potential clinical application in treating pancreatic ductal adenocarcinoma” J. Carcinogene Mutagene. 2013, pp. 13:1-8.
[3] P. Toopmuang, C. Khamchum, and V. Punsuvon, “Detection and confirmation of hispolon in the mushroom Phellinus linteus” 2014, J. Sci. Asia. Vol. 40, pp. 141-144.
[4] B.K. Yang, S.L. Hwang, I.K. Yun, E.J. Do, W.H. Lee, Y.M. Jung, S.C. Hong and D.C. Park, “Antitumor effects and immunodulating activities of Phellinus linteus extract in a CT-26 cell-injected colon cancer mouse model” Mycobiol. 2009, Vo. 37, pp 128-132.
[5] D.S. Šeklić, M.S. Stanković, M.G. Milutinović, M.D. Topuzović, A. Š. Štajn and S.D. Marković, “Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines” Arch. Biol. Sci. 2016, Vol.68, pp. 93-105.
[6] C.J. Lin, H.M. Lien, H.Y. Chang, C.L. Huang, J.J. Liu, Y.C. Chang, C.C. Chen and C.H. Lai, “Biological evaluation of Phellinus linteus-fermented broths as anti-inflammatory agents” J. Biosci. Bioeng. 2014. Vo. 118, pp. 88-93.
[7] M. Balouiri, M. Sadiki, and S.K. Ibnsouda, “Methods for in vitro evaluating antimicrobial activity: A review” J. Pharm. Anal. 2016, Vol. 6, pp. 71-19.
[8] H. Chen, Tian, T. Miao, H. and Zhao, Y.Y. “Traditional uses, fermentation, phytochemistry and pharmacology of Phellinus linteus: A review” Fitoterapia, Vol. 113, pp 6-26.
[9] C. Valgas, S.M. Souza, E.F.A. Smânia, and A. Smânia-Jr, “Screening methods to determine antibacterial activity of natural products” Bra. J. Microbiol. 2007, pp. 369-379.
[10] W.S. Jo, R. Y.H., Choi, S.G., Seo, G.S., Sung, J.M. and Uhm, J.Y. “the culture conditions for mycelial growth of Phellinus spp.” Mycobiol. 2006, pp. 200-205.
[11] Samchai, S., Seephonkai, P., Sangdee, A., Puntumchai, A. and Klinhom, U. Antioxidant, cytotoxic and antimalarial activities from crude extracts of mushroom Phellinus linteus. J. Biol. Sci. 2009;783-778.
[12] C. Sittiwet, and D. Puangpronpitag, “Anti-Streptococcus aureus activity of Phellinus igniarius aqueous extract” In. J. Pharm. 2008, Vol. 4, PP. 503-505.
[13] S.B. Hong, M.H. Rhee, B.S. Yun, Y.H. Lim, H.G. Song, and K.S. Shin, “Synergistic anti-bacrteial effects of Phellinus baumii ethyl acetate extracts and lactam antimicrobial agents against methicillin-resistant Staphylococcus aureus” Ann. Lab. Med. 2016, Vol. 36, pp. 111-116.
[14] M.J. Alves, I.C.F.R. Ferreira, H.J.C. Froufe, R.M.V. Abreu, A. Martins, and M. Pintado, “Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies” J. Appl. Microbiol. 2013, pp. 1-12.