Extracellular Laccase Production by Co-culture between Galactomyces reesii IFO 10823 and Filamentous Fungal Strains Isolated from Fungus Comb Using Natural Inducer

Authors: P. Chaijak, M. Lertworapreecha, C. Sukkasem

Abstract:

Extracellular laccases are copper-containing microbial enzymes with many industrial biotechnological applications. This study evaluated the ability of nutrients in coconut coir to enhance the yield of extracellular laccase of Galactomyces reesii IFO 10823 and develop a co-culture between this yeast and other filamentous fungi isolated from the fungus comb of Macrotermes sp. The co-culture between G. reesii IFO 10823 and M. indicus FJ-M-5 (G3) gave the highest activity at 580.20 U/mL. When grown in fermentation media prepared from coconut coir and distilled water at 70% of initial moisture without supplement addition, G3 produced extracellular laccase of 113.99 U/mL.

Keywords: Extracellular laccase, production, yeast, natural inducer.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1129175

References:

[1] A. Gupte, D. Madamwar, 1997. Solid state fermentation of lignocellulosic waste for cellulose and -glucosidase production by cocultivation of Aspergillus ellipticus and Aspergillus fumigatus. Biotechnology progress 13, 166-169.
[2] A. Pendey, 1992. Recent process development in solid state fermentation. Process biochemistry 27, 109-117.
[3] Asia and pacific coconut community, 2016. Country profile of Thailand. http://www.apccsec.org/apccsec/media.php?page=country&id=3.Accessed on 01/10/2016
[4] B. Bertrand, F. Martinez-Morales, R. Tinoca-Valencia, S. Rojas, L. Acosta-Urdapilleta, M.R. Trejo-Hernandez, 2015. Biochemical and molecular characterization of laccase isoforms produced by the white-rot fungus Trametes versicolor under submerged culture conditions. Journal of molecular catalysis B: Enzymatic 122, 339-347.
[5] C.H. Neoh, A. Yahya, R. Adnan, Z.A. Majid, and Z. Ibrahim, 2013. Optimization of decolorization of palm oil mill effluent (POME) by growing cultures of Aspergillus fumigatus using response surface methodology. Environmental Science and Pollution Research 20, 2912-2923.
[6] C.H. Neoh, C.Y. Lam, C.K. Lim, A. Yahya, and Z. Ibrahim, 2014. Decolorization of palm oil mill effluent using growing cultures of Curvularia clavata. Environmental Science and Pollution Research 21, 4397-4408.
[7] D.K. Sahoo, R. Gupta, 2005. Evaluation of ligninolytic microorganisms for efficient decolorization of a small pulp and paper mill effluent. Process biochemistry 40, 1573-1578.
[8] E. Birhanli, S. Erdogan, O. Yesilada, Y. Onal, 2013. Laccase production by newly isolated white rot fungus Funalia trogii: Efffect of immobilization matrix on laccase production. Biochemical engineering journal 71, 134-139.
[9] F.E. Vega, M. Blackwell, 2005. Insect-fungal associations: ecology and evolution (United State of America: Oxford university press).
[10] J.D. Castano, C. Cruz, E. Torres, 2015. Optimization of production, purification and characterization of a laccase from the native fungus Xylaria sp. Biocatalysis and agricultural biotechnology 4, 710-716.
[11] P. Chaijak, M. Lertworapreecha, C. Sukkasem, 2017. Decolrization and phenol removal of palm oil mill effluent by termite associated yeast. International Conference on Pollution Control and Waste Management. Dubai, UAE during January 30-31, 2017.
[12] F. Hyo do, T. Inoue, J. I. Azuma, I. Tayasu, T. Abe, 2000. Role of the mutualistic fungus in lignin degradation in the fungus-growing termite Macrotermes gilvus (Isoptera; Macrotermitinae). Soil biology and biochemistry 32, 653-658.
[13] F. Hyodo, T. Inoue, J. I. Azuma, T. Kudo, T. Abe, 2003. Differential role of symbiotic fungi in lignin degradation and food provision for fungus-growing termites (Macrotermitinae: Isoptera). Functional ecology 17, 186-193.
[14] G. Rohrman, 1978. The origin, structure, and nutritional importance of the comb in two species of Macrotermitinae. Pedobiologia 18, 89-98.
[15] G. Rouland-Lefevre, 2000. Symbiosis with fungi. In Termites: evolution, sociality, symbioses, ecology, T. Abe, D.E. Bignell, M. Higashi, ed. (Dordrecht: Kluwer Academic Publisshers) 289-306.
[16] M. Abad, P. Noguera, R. Puchades, A. Maquieira, V. Noguera, 2002. Physiochemical and chemical properties of some coconut coir dusts for use as a peat substitute for containerized ornamental plants. Bioresoure technology 82, 241-245.
[17] M.I. Fonseca, E. Shimizu, P.D. Zapata, L.L. Villalba, 2010. Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina). Enzyme and microbial technology 46, 534-539.
[18] M. Smolander, H. Boer, M. Valkiainen, R. Roozeman, M. Bergelin, J. Eriksson, X. Zhang, A. Koivulaand, L. Viikari, 2008. Development of a printable laccase-based biocathode for fuel cell applications. Enzyme and Microbial Technology 43, 93-102.
[19] P.K.A. Muniswaran, P. Selakumar, N.C.I.M. Charyulu, 1994. Production of cellulose from coconut coir pith in solid state fermentation. Journal of chemical technology and biotechnology 60, 147-161.
[20] P. Muensri, T. Kunanopparat, P. Menut, S. Siriwattanayotin, 2011. Effect of lignin removal on the properties of coconut coir fiber/wheat glutan biocomposite. Composites: Part A 42, 173-179.
[21] R.R. Mohammed, 2013. Decolorisation of Biologically Treated Palm Oil Mill Effluent (POME) Using Adsorption Technique. International Refereed Journal of Engineering and Science (IRJES) 2, 1-11.
[22] R.P. Keiwin, 2008. Copper facts, (Emvironmetal Protection Agency: United Utate of Ameriac) 1-5.
[23] S. Bakshian, H.R. Kariminia, 2011. Development of laccase and manganese peroxidase biocathodes for microbial fuel cell applications. World renewable congress. Linkoping, Sweden during May 8-11, 2011.
[24] S. Rehman, H. Aslam, A. Ahmad, S.A. Khan, M. Sohail, 2015. Production of plant cell wall degrading enzymes by monoculture and co-culture of Aspergillus niger and Aspergillus terreus under SSF of banana peels. Brazilian journal of microbiology 45, 1485-1492.
[25] T. Nobre, C. Rouland-Lefevre, D.K. Aanen, 2010. Comparative biology of fungus cultivation in termites and ants. In Biology of Termites: a Modern Synthesis, D.E. Bignell, Y. Roisin, and N. Lo, ed. (Netherlands: Springer), 193-210.
[26] Y. Taprab, T. Johjima, Y. Maeda, S. Moriya, S. Trakulnaleamsai, N. Noparatnaraporn, M. Ohkuma, T. Kudo, 2005. Symbiotic Fungi Produce Laccases Potentially Involved in Phenol Degradation in Fungus Combs of Fungus-Growing Termites in Thailand. Applied Environmental Microbiology 71, 7696-7704.