The Use of SD Bioline TB AgMPT64® Detection Assay for Rapid Characterization of Mycobacteria in Nigeria
Performing culture and characterization of mycobacteria in low resource settings like Nigeria is a very difficult task to undertake because of the very few and limited laboratories carrying out such an experiment; this is a largely due to stringent and laborious nature of the tests. Hence, a rapid, simple and accurate test for characterization is needed. The “SD BIOLINE TB Ag MPT 64 Rapid ®” is a simple and rapid immunochromatographic test used in differentiating Mycobacteria into Mycobacterium tuberculosis (NTM). The 100 sputa were obtained from patients suspected to be infected with tuberculosis and presented themselves to hospitals for check-up and treatment were involved in the study. The samples were cultured in a class III Biosafety cabinet and level III biosafety practices were followed. Forty isolates were obtained from the cultured sputa, and there were identified as Acid-fast bacilli (AFB) using Zeihl-Neelsen acid-fast stain. All the isolates (AFB positive) were then subjected to the SD BIOLINE Analyses. A total of 31 (77.5%) were characterized as MTBC, while nine (22.5%) were NTM. The total turnaround time for the rapid assay was just 30 minutes as compared to a few days of phenotypic and genotypic method. It was simple, rapid and reliable test to differentiate MTBC from NTM.
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 WHO 2006. Global tuberculosis control: Surveillance, planning, financing. World Health Organization. Geneva. WHO/HTM/TB/2006.
 Wim, H., Van B., Abdullahi, D. B and Mohammed G (2004). Kaduna State tuberculosis and leprosy control Programme. National Tuberculosis and Leprosy Control Programme: Project agreement, Kaduna, pp36.
 Wagner D, Young L. (2004). Non-Tuberculous Mycobacterial infections: A clinical review, Infection 2004: 32; 257 – 70.
 Hasegawa N, Miura T, Ishii K, Yamaguchi TH, Lindner S, Meritt JD (2002). New Sample and rapid test for culture of Mycobacterium tuberculosis complex: A multi-centeric study. J. Clin. Microbiol 40: 908 – 12.
 Steingart KR, Dendukuri N, Henry M et al (2009). Performance of purified antigens for sero-diagnosis of pulmonary tuberculosis: A meta-analysis. Clin vaccine Immunol 16: 260 – 76.
 Andersen P, Askgaad D, Ljingqvist L, et al. (1991). Proteins released from Mycobacterium tuberculosis during growth. Infect Immun 59: 1905 – 10.
 Abe C, Hirano K, Tomiyama T. (1999). Simple and rapid identification Mycobacterium tuberculosis complex by Immunochromatographic assay using anti MPB64 monoclonal antibodies. J. Clin Microbiol 37: 3693 – 7.
 Fur, Wang C, Shi C, Lu M, Fang Z, Lu J. et al (2009). An improved whole blood gamma interferon assay based on the CFP 21 – MPT 64 fusion protein. Clin. Vaccine immunol 16: 686 – 91.
 Akyar I, Kocagoz T, Sinik G, Okten S, Aytekin N, Kocagoz S, (2010). Lateral flow assay for rapid differentiation of Mycobacterium tuberculosis complex and 97 species to mycobacteria other than tuberculosis grown n LJ and TK-SLC medium. Indian Journal of Med Microbiol. 28(4): 481 – 4.
 Martin A, Bombeek D, Muldirs W, Fissette K, De Rijk P, Palomino JC (2011). Evaluation of the TB Ag MPT 64 Rapid test for the identification of Mycobacterium tuberculosis complex. Int. J. Tuberc Lung Dis 15(5): 703 – 5.
 Gennaro ML (2000). Immunologic Diagnosis of Tuberculosis. Proceedings of International Symposium on Tuberculosis Vaccine Development and Evaluation. J. Infect Dis 30(3): 243.
 Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories: Recommendations for CDC-Convened Biosafety Blue Ribbbon Panel (2012). Centre for Disease Control, Atlanta, Georgia, USA.
 National Agency for the control of AIDS NACA 2012. Global AIDS Research Progress Report 2012.
 Gaillard T, Fabre M, Martinaud C, Vong R, Brisou P and Solar C (2011). Assessment of SD Bioline AgMPT64 RapidTM and the MGITTM TBC Identification tests for the diagnosis of tuberculosis. Diagn Microbiol. Infect Dis. 70:154-156.
 Hillemann D, Rusch-Gerades S, and Ritcher E (2005). Application of the capilia TB assay for culture confirmation of M tuberculosis complex isolates. Int. J. Tuberc. Lung Dis, 9:1409-1411.