Haematology and Serum Biochemical Profile of Laying Chickens Reared on Deep Litter System with or without Access to Grass or Legume Pasture under Humid Tropical Climate

Authors: E. Oke, A. O. Ladokun, J. O. Daramola, O. M. Onagbesan

Abstract:

There has been a growing interest on the effects of access to pasture on poultry health status. However, there is a paucity of data on the relative benefits of grass and legume pastures. An experiment was conducted to determine the effects of rearing systems {deep litter system (DL), deep litter with access to legumes (LP) or grass (GP) pastures} haematology and serum chemistry of ISA Brown layers. The study involved the use of two hundred and forty 12 weeks old pullets. The birds were reared until 60 weeks of age. Eighty birds were assigned to each treatment; each treatment had four replicates of 20 birds each. Blood samples (2.5 ml) were collected from the wing vein of two birds per replicate and serum chemistry and haematological parameters were determined. The results showed that there were no significant differences between treatments in all the parameters considered at 18 weeks of age. At 24 weeks old, the percentage of heterophyl (HET) in DL and LP were similar but higher than that of GP. The ratio of H:L was higher (P<0.05) in DL than those of LP and GP while LP and GP were comparable. At week 38 of age, the percentage of PCV in the birds in LP and GP were similar but the birds in DL had significantly lower level than that of GP. In the early production phase, serum total protein of the birds in LP was similar to that of GP but higher (P<0.05) than that of DL. At the peak production phase (week 38), the total protein in GP and DL were similar but significantly lower than that of LP. The albumin level in LP was greater (P<0.05) than GP but similar to that of DL. In the late production phase, the total protein in LP was significantly higher than that of DL but similar to that of GP. It was concluded that rearing chickens in either grass or legume pasture did not have deleterious effects on the health of laying chickens but improved some parameters including blood protein and HET/lymphocyte.

Keywords: Rearing systems, Stylosanthes, Cynodon serum chemistry, haematology, hen.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1339830

References:

[1] Latimer, K. S. and Bienzle, D. 2000. Determination and interpretation of the avian leukogram,” in Schalm's Veterinary Hematology, B. F. Feldman, J. G. Zinkl, and N. C. Jain, Eds., pp. 417–432, LippincottWilliams &Wilikins, Philadelphia, Pa, USA.
[2] Suchy. P., Strakova, E., Jarka, B., Thiemel, J., Vecerek, V. 2004. Differences between metabolic profiles of egg-type and meat-type hybrid hens. Czech Journal of Animal Science, 49: 323-328.
[3] Pavlik, A., Pokludova, M., Zapletal, D., Jelinek, P. 2007) Effects of housing systems on biochemical indicators of blood plasma in laying hens. Acta Vet Brno 76: 339-347.
[4] Nazifi, S., Mosleh, N., Ranjbar, V.R., Khordadmehr, M. 2012. Reference values of serum biochemical parameters in adult male and female ring-necked pheasants. Comp Clin Pathol 21: 981-984.
[5] Suchy P, Strakova E, Kroupa L, Steinhauser L, Herzig I. 2010. Values of selected biochemical and mineral metabolism indicators in feathered game. Acta Vet Brno 79: 9-12.
[6] Kececi T, Co¨l R (2010) Haematological and biochemical values of the blood of pheasants (Phasianus colchicus) of different ages. Turk J Vet Anim Sci 35: 149-156.
[7] Varley, H., Van, E. and Kass I., 1980. Practical clinical chemistry, New York, Inter Science Publishers Inc. 197 – 240
[8] Bousnes, R., Taussky, H.H., 1945. Colorimetric determination of creatinine by Jaffe reaction. Journal of Biochemistry, 158, 581–591.
[9] Menon, D. G. Bennett, D. C. Schaefer, A. M. and Chengm K. M. 2013.Hematological and serum biochemical profile of farm emus (Dromaius novaehollandiae) at the onset of their breeding season. Poultry Science 92 :935–944.
[10] Dal Bosco, A. Castellini, C. 2009. Effect of rearing system and season on the performance and egg characteristics of Ancona laying hens. Ital. J.Anim.Sci. vol. 8, 175-188.
[11] Wang, K. H., S. R. Shi, T. C. Dou, and H. J. Sun. 2009. Effect of a free-range raising system on growth performance, carcass yield, and meat quality of slow-growing chicken. Poult. Sci. 88:2219–2223.
[12] Blokhuis, H., E. Ekkel, S. Korte, H. Hopster, and C. Van Reenen.2000. Farm animal welfare research in interaction with society.Vet. Q. 22:217–222.
[13] Fanatico, A., P. Pillai, L. Cavitt, C. Owens, and J. Emmert. 2005. Evaluation of slower-growing broiler genotypes grown with and without outdoor access: Growth performance and carcass yield. Poult. Sci. 84:1321–1327.
[14] Chen , X. Jiang, W., Tan, H. Z., Xu, G. F. , Zhang, X. B., Wei, S., and Wang, X. Q. 2013. Effects of outdoor access on growth performance, carcass composition, and meat characteristics of broiler chickens. Poultry Science, 92 :435–443.
[15] Casagrande, P. P., Passamonti, F., Asdrubali, G., 2001. La gallina ovaiola allevata a terra e in gabbia. Riv. Avicolt. 3(5-6):12-15.
[16] Minelli, G., Sirri, F., Folegatti, E., Meluzzi, A., Franchini, A., 2007. Egg quality traits of laying hens reared in organic and conventional systems. Ital. J. Anim. Sci. 6(Suppl.1):728-730.
[17] Rossi, M., 2007. Influence of the laying hen housing systems on table egg characteristics. pp 49-51 in Proc. 18th Eur. Symp. WPSA on the Quality of Eggs and Egg Products, Prague, Czech Republic.
[18] Kakade, M. L. and Evans, R. J. 1966. Growth inhibitors of rats fed navy bean fraction Phaseolus vulgaris. Journal of Nutrition. 90: 191-198.
[19] Maxwell, M.H. and Robertson, G.M. 1998. The avian heterophil leucocyte: a review. World’s Poultry Science Journal.; 54:155-178.
[20] Alabi ,O. M., Aderemi, F. A. and Adeniji, O. B. 2015. Effect of Alternative Housing Systems on Blood Profile of Egg-type Chickens in Humid Tropics. American Journal of Experimental Agriculture, 7:197-204.
[21] Talebi, A.., Asri-Rezaei, S., Roszeh-Chai, R. and Sahraei, R. 2005. Comparative studies on haematological values of broilers strains. International Journal of Poultry Science, 5: 573-579.
[22] Sobayo, R. A., Oguntona, E. B., Adeyemi, O. A., Fafiolu, O. A., Oso, A. O., Osionowo, O. A., Sales, J. and Mellet, F. D. 2008. Post-mortem pH decline in different ostrich muscles. Meat Science, 42: 235-238.
[23] Husak, R., Sebranek, J., Bregendahl, K. 2008. A survey of commercially available broilers marketed as organic, free-range, and conventional broilers for cooked meat yields, meat composition, and relative value. Poult. Sci. 87:2367–2376.
[24] Oke, O. E., Ladokun, A. O. and Onagbesan, O. M. Reproductive performance of layer chickens reared on deep litter system with or without access to grass or legume pasture. Journal of Animal Physiology and Animal Nutrition 100 (2016) 229–235.
[25] Benjamin, M. M., 1985. Outline of veterinary clinical pathology. 3rd Ed. The Iowa State Uni. Press, Ames.
[26] Bernard, F. F., Joseph, G. Z. and Jain, N. C. 2000. Schalm’s Veterinary Hematology. 5th Ed. Lippincott Williems and Wilkins, Philadelphia.
[27] Brar, R.S., H.S. Sandhu and A. Singh, 2002. Veterinary clinical diagnosis by laboratory methods. 1st Ed. Kalyani Publishers, New Delhi.
[28] Fossati, P., Principe, L. and G. Berti 1980. Use of 3,5-dichloro-2-hydroxybenzene sulfonic acid/4-aminophenazone chromogenic system in the direct enzymic assay of uric acid in serum and urine. Clin. Chem. 26:227–231.
[29] Stockham, S. L. and M.A. Scott, 2002. Fundamentals of veterinary clinical pathology. Iowa State Press, A Blackwell Publishing Company.
[30] Ikegwuonu, F.I. and Bassir, 0. 1977. Effect of phytohaemaglutinins from immature legume seeds on Ihe function and enzyme activities of the liver on the histopathological changes of some organs of the rat. Toxicol. Appl. Pharma., 40 : 21 7-226.
[31] Olugbemi, T. S., Mutayoba, S. K. and Lekule, F. P. 2010. Effect of Moringa (Moringa oleifera) Inclusion in Cassava-Based Diets Fed to Broiler Chickens. Int. J. Pout. Sci. 9: 363-367.
[32] Puvadolpirod, S., and J. P. Thaxton. 2000. Model of physiological stress in chickens. 1. Response parameters. Poult. Sci. 79:363–369.