Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30840
Vitamin C Status and Nitric Oxide in Buffalo Ovarian Follicular Fluid in Relation to Seasonal Heat Stress and Phase of Estrous Cycle

Authors: H. F. Hozyen, A. M. Abo-El Maaty


Heat stress is a recognized problem causing huge economic losses to the buffalo breeders as well as dairy industry. The aim of the present work was to study the pattern of vitamin C and nitric oxide in follicular fluid of buffalo during different seasons of the year considering phase of estrous cycle. This study was conducted on 208 cyclic buffaloes slaughtered at Al-Qaliobia governorate, Egypt, over one year. The obtained results revealed that vitamin C in follicular fluid was significantly lower in summer than winter and spring. On the other hand, nitric oxide (NO) was significantly higher in summer and autumn than winter and spring. Both vitamin C and NO did not differ significantly between follicular and luteal phases. In conclusion, the present study revealed that alterations in concentrations of follicular fluid vitamin C and NO that occur in summer could be related to low summer fertility in buffalo.

Keywords: vitamin C, buffalo, follicular fluid, NO and heat stress

Digital Object Identifier (DOI):

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 836


[1] S. H. Abdel-Aziem, L. M. Salem, M. S. Hassanane, K. F. Mahrous, "Genetic analysis between and within three Egyptian water buffalo populations using RAPD-PCR", J. Am. Sci., vol 6, 2010, pp 217-226.
[2] I. Soliman, H. Bassiony, "Role of buffalo in international trade", J. Agric. Sci. Technol., B1, 2011, pp 799-809.
[3] I. F. Marai, A. A. Habeeb, "Buffalo's biological functions as affected by heat stress", Livest. Sci., vol 12, 2010, pp 89-109.
[4] A. Kumar, S. Mehrotra, G. Singh, K. Narayanan, G. K. Das, Y. K. Soni, M. Singh, A. S. Mahla, N. Srivastava, M. R. Verma, "Sustained delivery of exogenous melatonin influences biomarkers of oxidative stress and total antioxidant capacity in summer-stressed anestrous water buffalo (Bubalus bubalis)", Theriogenology, vol 83, 2015, pp 1402–1407.
[5] M. M. Nour El-Din, "Proposed climate change adaptation strategy for the ministry of water resources & irrigation in Egypt". Joint programme for climate change risk management in Egypt.
[6] Payne, W.J.A., 1990. An Introduction to Animal Husbandry in the Tropics. Longman Scientific and Technical, England.
[7] R. S. Manik, P. Palta, S. K. Singla, V. Sharma, "Folliculogenesis in buffalo (Bubalus bubalis)". Reprod. Fertil. Dev., vol 14, 2002, pp 315-325.
[8] K. H. El-Shahat, M. Kandil, "Antioxidant capacity of follicular fluid in relation to follicular size and stage of estrous cycle in buffaloes", Theriogenology, vol 77, 2012, pp 1513-1518.
[9] J. L. Leroy, T. Vanholder, J. R. Delanghe, G. Opsomer, A. Van Soom, P. E. Bols, J. Dewulf, A. de Kruif, "Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum", Theriogenology, vol 62, 2004, pp 1131-1143.
[10] H. H. Elkhidir, "Vitamin C status in Sudanese camels". PhD thesis. Utrecht Univ., 2002; 98 p.
[11] S. Nayyar, R. Jindal, "Essentiality of antioxidant vitamins for ruminants in relation to stress and reproduction", Iran. J. Vet. Res., vol 11, No. 1, Ser. No. 30, 2010.
[12] B. Serpek, N. Baspinar, S. Haliloglu, H. Erdem, "The relationship between ascorbic acid, oestradiol 17β and progesterone in plasma and in ovaries during the sexual cycle in cattle", Revue Méd. Vét., vol 152, 2001, pp 253-260.
[13] W. A. Razzaque, R. Ali, A. Hussain, M. Mutha Rao, S. Naafia, U. Sharma, K. Hussain, "Follicular fluid nitric oxide concentration in relation to stages of estrus cycle in ewes", Indian Vet. J., vol 91,2014, pp 35 – 38.
[14] G. O. Zamberlam, "The involvement of nitric oxide in bovine follicular development and ovulation", PhD Thesis, 2013, Université de Montréal.
[15] A. Jablonka-Shariff, L. M. Olson, "Nitric oxide is essential for optimal meiotic maturation of murine cumulus-oocyte complexes in vitro", Mol. Reprod. Dev., 2000, vol 55, pp 412-421.
[16] K. M. Dhandapani, D. W. Brener, "The role of glutamate and nitric oxide in the reproductive neuroendocrine system", Biochem. Cell Biol., 2000, vol 78, pp 165-179.
[17] K. R. L. Schwarz, R. L. Pedro, Pires, Paulo, R., Adona, T. H., Camara de Bem., Claudia, L. and Leal, V., "Influence of nitric oxide during maturation on bovine oocyte meiosis and embryo development in vitro", Reprod. Fertil. Dev., vol 20, 2008, pp 529-536.
[18] A. Revelli, L. D. Piane, S. Casano, E. Molinari, M. Massobrio, P. Rinaudo, "Follicular fluid content and oocyte quality: from single biochemical markers to metabolomic", Reprod. Biol. Endocrinol., 2009, 7:40.
[19] S. Mondal, V. Kumar, I.J. Reddy, S. Khub, "Progesterone and nucleic acid content of buffalo corpus luteum in relation to stages of estrus cycle". Indian J. Anim. Sci., 2004, vol 74, pp 710–712.
[20] P. Jaglan, G.K. Das, B.V. Sunil Kumar, R. Kumar, F.A. Khan, S.K. Meur, "Cyclical changes in collagen concentration in relation to growth and development of buffalo corpus luteum", Vet. Res. Commun., 2010, vol 34, pp 511–518.
[21] L. J. Harris, S. N. Ray, Lancet. 1935, vol 1, pp 71- 462
[22] H. A. C. Montgomery, J. F. Dymock, Analyst,1961, vol 86, pp 414.
[23] M. Tanaka, Y. Kamiya, M. Kamiya, Y. Nakai, “Effect of high environmental temperatures on ascorbic acid, sulfhydryl residue and oxidized lipid concentrations in plasma of dairy cows,” Anim. Sci. J., 2007, vol. 78, no. 3, pp 301–306.
[24] G. A. Megahed, M. M. Anwar, S. I. Wasfy, M. E. Hammadeh, “Influence of heat stress on the cortisol and oxidant antioxidants balance during oestrous phase in buffalo-cows (Bubalus bubalis): thermo-protective role of antioxidant treatment”, Reprod. Domest. Anim., 2008, vol. 43,no. 6, pp.672– 677.
[25] M. Sakatani, A. Z. Balboula, K. Yamanaka, and M. Takahashi, “Effect of summer heat environment on body temperature, estrous cycles and blood antioxidant levels in Japanese black cow”, Anim. Sci. J., 2012, vol. 83, no. 5, pp. 394–402.
[26] F. H. Thomas, R. Leask, V. Srsen, S. C. Riley, N. Spears, E. E. Telfer, "Effect of ascorbic acid on health and morphology of bovine preantral follicles during long-term culture", Reproduction, vol 122, 2001, pp 487–495.
[27] H. A. Hussein, M. R. Abd Ellah, D. R. I. Derar, "Antioxidant concentrations in serum, follicular fluid, and corpus luteum of cyclic buffalo cows", Comp. Clin. Pathol., vol 22, 2013, pp 829–833.
[28] H. F. Hozyen, H. A. Hodallah, G. E. S. Essawy, S. I. A. Shalaby, "Seasonal changes in some oxidant and antioxidant parameters during folliculogenesis in Egyptian buffalo", Anim. Reprod. Sci., vol 151, 2014, pp 131-136.
[29] O. S. Afify, M. N. Makled, "Effect of ascorbic acid on productive and reproductive performance of Bouscat rabbits exposed to heat stress", First Egyptian-Hungarian Conference. Alexandria, Egypt. 17-19 September 1996, pp. 313.
[30] S. Nayyar, R. Jindal, "Essentiality of antioxidant vitamins for ruminants in relation to stress and reproduction", Iran. J. Vet. Res., 2010, vol. 11, no. 1, Ser. no. 30.
[31] P. J. Hansen, "Effects of heat stress on mammalian reproduction", Phil. Trans. R. Soc. B, vol 364, 2009, 3341-3350.
[32] B. I. Van Voorhis, M. S. Dunn, G. D. Synder, C. P. Weiner, "Nitric oxide: an autocrine regulator of human granulosa-luteal cell steroidogenesis", Endocrinology, vol 135, 1994, pp1799-806.
[33] S. Naafia, W. A. A. Razzaque, M. Mutha Rao, and K. Hussain, "Nitric oxide profiles in serum and follicular fluid of cyclic and acyclic sheep", J. Res. Dev., 2011, vol. 11, pp 199-128.
[34] M. Rosselli, P. J. Keller, R. K. Dubey, "Role of nitric oxide in the biology, physiology and pathophysiology of reproduction", Hum. Reprod. Update, 1998, vol 4, pp 3-24.
[35] H. S. Lucky, S. Gupta, Y. Kim, A. Agarwal, "Female infertility and antioxidants", Curr. Womens Health Rev., vol 6, 2010, 84-95.
[36] F. A. Khan, G. K. Das., "Follicular characteristics and intrafollicular concentrations of nitric oxide and ascorbic acid during ovarian acyclicity in water buffalo (Bubalus bubalis)". Trop. Anim. Health Prod., 2012, vol 44, pp 125-131.
[37] A. B. Motta, A. Estevez, M. F. de Gimeno, "The involvement of nitric oxide in corpus luteum regression in the rat: feedback mechanism between prostaglandin F(2alpha) and nitric oxide". Mol. Hum. Reprod., 1999, vol 5, no 11, pp 1011-1016.
[38] P. K. Dubey, V. Tripathi, R. P. Singh, G. T.Sharma, "Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles". J. Vet. Sci., 2011, vol 12, no 3, pp 257-265.