Resveratrol Incorporated Liposomes Prepared from Pegylated Phospholipids and Cholesterol
Liposomes and pegylated liposomes were widely used as drug delivery system in pharmaceutical field since a long time. However, in the former time, polyethylene glycol (PEG) was connected into phospholipid after the liposomes were already prepared. In this paper, we intend to study the possibility of applying phospholipids which already connected with PEG and then they were used to prepare liposomes. The model drug resveratrol was used because it can be applied against different diseases. Cholesterol was applied to stabilize the membrane of liposomes. The thin film technique in a laboratory scale was a preparation method. The liposomes were then characterized by nanoparticle tracking analysis (NTA), photon correlation spectroscopy (PCS) and light microscopic techniques. The stable liposomes can be produced and the particle sizes after filtration were in nanometers. The 2- and 3-chains-PEG-phospholipid (PL) caused in smaller particle size than the 4-chains-PEG-PL. Liposomes from PL 90G and cholesterol were stable during storage at 8 °C of 56 days because the particle sizes measured by PCS were almost not changed. There was almost no leakage of resveratrol from liposomes PL 90G with cholesterol after diffusion test in dialysis tube for 28 days. All liposomes showed the sustained release during measuring time of 270 min. The maximum release amount of 16-20% was detected with liposomes from 2- and 3-chains-PEG-PL. The other liposomes gave max. release amount of resveratrol only of 10%. The release kinetic can be explained by Korsmeyer-Peppas equation.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1127062Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 713
 Suk, J.S., Xu, Q., Kim, N., Hanes, J., Ensign, L.M., PEGylation as a strategy for improving nanoparticle-based drug and gene delivery, Adv Drug Deliv Rev 2016 (1;99), 28-51.
 Kneidl, B., Peller, M., Winter, G., Lindner, L.H., Hossann, M., Thermosensitive liposomal drug delivery systems: state of the art review, Int J Nanomedicine 2014 Sep (16;9), 4387-98.
 Celares GmbH, ZIM Report of Project Number KF2883001 (PEGPHOS).
 Kumpugdee-Vollrath, M., Ibold Y., Increasing solubility of poorly water soluble drug resveratrol by surfactants and cyclodextrins, Advanced Materials Research, 2012 (418-420), 2231-2234.
 Malvern Instruments GmbH. Nanoparticle Tracking Analysis/NanoSight linie; http://www.malvern.com/de/products/technology/nanoparticle-tracking-analysis
 Carr, B., Hole, P., Malloy, A., Nelson, P., Smith, J., Applications of Nanoparticle Tracking Analysis (NTA) in Nanoparticle Research, a Mini-Review, NanoSight Ltd., Amesbury, 2009.
 Römmp Online Database; https://roempp.thieme.de
 Helmis, M., Mohamad, B., Kumpugdee-Vollrath, M., (2016) Influence of Several Excipients on Drug Release of Tablets Containing Resveratrol, M J Pharma 2016 1(1): 007, 1-7.