Commenced in January 2007
Paper Count: 30127
Fabrication of Immune-Affinity Monolithic Array for Detection of α-Fetoprotein and Carcinoembryonic Antigen
Abstract:In this paper, we presented a highly sensitive immune-affinity monolithic array for detection of α-fetoprotein (AFP) and carcinoembryonic antigen (CEA). Firstly, the epoxy functionalized monolith arrays were fabricated using UV initiated copolymerization method. Scanning electron microscopy (SEM) image showed that the poly(BABEA-co-GMA) monolith exhibited a well-controlled skeletal and well-distributed porous structure. Then, AFP and CEA immune-affinity monolithic arrays were prepared by immobilization of AFP and CEA antibodies on epoxy functionalized monolith arrays. With a non-competitive immune response format, the presented AFP and CEA immune-affinity arrays were demonstrated as an inexpensive, flexible, homogeneous and stable array for detection of AFP and CEA.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1126744Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1361
 B. Joshua, O. Miguel, K. Yordan, A.B. Hugh, R. Avraham, “Lensless CCD-based fluorometer using a micromachined optical Söller collimator”, Lab Chip, vol. 11, pp. 941-949, 2011.
 S. Parween, P. Nahar, “Image-based ELISA on an activated polypropylene microtest plate—A spectrophotometer-free low cost assay technique”, Biosens. Bioelectron., vol. 48, pp. 287-292, 2013.
 M. Mirasoli, M. Guardigli, E. Michelini, A. Roda, “Recent advancements in chemical luminescence-based lab-on-chip and microfluidic platforms for bioanalysis”, J. Pharmaceut. Biomed. Anal., vol. 87, pp. 36-52, 2014.
 J. Horak, C. Dincer, H. Bakirci, G. Urban, “A disposable dry film photoresist-based microcapillary immunosensor chip for rapid detection of Epstein–Barr virus infection”, Sensor. Actuat. B Chem., vol. 191, pp. 813-820, 2014.
 L. Li, Y. Lu, Z.J. Bie, H.Y. Chen, Z. Liu, “Photolithographic Boronate Affinity Molecular Imprinting: A General and Facile Approach for Glycoprotein Imprinting”, Angew. Chem. Int. Ed., vol. 52, pp. 7451-7454, 2013.
 C. Cafer, P. F. Jerome, P. L. James, C. Perihan, “Development of a micro-total analysis system (μ-TAS) for the determination of catecholamines”, Anal. Bioanal. Chem., vol. 398, pp. 1909-1917, 2010.
 Y.C. Liu, Y. Lu, Z. Liu, “Restricted access boronate affinity porous monolith as a protein A mimetic for the specific capture of immunoglobulin G”, Chem. Sci., vol. 3, pp. 1467-1471, 2012.
 L. Li, X. D. Bi, M. Feng, J. T. Zhu, “UV-initiated copolymerization route for facile fabrication of epoxy-functionalized micro-zone plates”, J. Applied Polymer Science, vol.131, pp. 39787-39793, 2014.
 L. Li, X. D. Bi, J. Z. Yu, C. L. Ren, Z. Liu, “A new soft lithographic route for the facile fabrication of hydrophilic sandwich microchips”, Electrophoresis, vol. 33, pp. 2591–2597, 2012.
 L. Li, Z.H. He, W.W. Wang, Y.M. Fan, X.P. Mao, D.H. Chen, C. Chen, “Synthesis of amino-functionalized epoxy acrylate film by magnetic-filtered plasma stream”, Prog. Org. Coat., vol. 56, pp. 126-130, 2006.
 L. Li, L. R. Xia, H. Y. Wang, X. D. Bi, “New method for fabricating an α-fetoprotein affinity monolithic polymer array”, J. Applied Polymer Science, vol. 132, pp. 41792-41796, 2015.
 Z. F. Fu, C. Hao, X. Q. Fei, H. X. Ju, “Flow-injection chemiluminescent immunoassay for α-fetoprotein based on epoxysilane modified glass microbeads”, Journal of Immunological Methods, vol. 312, pp. 61-67, 2006.