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Bioremediation of Phenanthrene by Monocultures and Mixed Culture Bacteria Isolated from Contaminated Soil

Authors: A. Fazilah, I. Darah, I. Noraznawati

Abstract:

Three different bacteria capable of degrading phenanthrene were isolated from hydrocarbon contaminated site. In this study, the phenanthrene-degrading activity by defined monoculture was determined and mixed culture was identified as Acinetobacter sp. P3d, Bacillus sp. P4a and Pseudomonas sp. P6. All bacteria were able to grow in a minimal salt medium saturated with phenanthrene as the sole source of carbon and energy. Phenanthrene degradation efficiencies by different combinations (consortia) of these bacteria were investigated and their phenanthrene degradation was evaluated by gas chromatography. Among the monocultures, Pseudomonas sp. P6 exhibited 58.71% activity compared to Acinetobacter sp. P3d and Bacillus sp. P4a which were 56.97% and 53.05%, respectively after 28 days of cultivation. All consortia showed high phenanthrene elimination which were 95.64, 79.37, 87.19, 79.21% for Consortia A, B, C and D, respectively. The results indicate that all of the bacteria isolated may effectively degrade target chemical and have a promising application in bioremediation of hydrocarbon contaminated soil purposes.

Keywords: Acinetobacter sp. P3d, Bacillus sp. P4a, consortia, phenanthrene, Pseudomonas sp. P6.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1126369

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References:


[1] A. Ovchinnikova, A. Vetrova, A. Filonov, A. Boronin, “Phenanthrene biodegradation and the interaction of Pseudomonas putida BS3701 and Burkholderia sp. BS3702 in plant rhizosphere”, Microbiology, vol. 78, no. 4, pp.433-439.2009.
[2] K., Nagarajan, K. C. Loh, “Formulation of microbial cocktails for BTEX biodegradation”, Biodegradation, vol 26, no.1, pp.51-63.2015.
[3] Y. M., Kim, C. K., Ahn, S. H., Woo, G. Y., Jung, and J. M. Park, “Synergic degradation of phenanthrene by consortia of newly isolated bacterial strains”, Journal of Biotechnology, vol. 144, no.4, pp.293-298.2009.
[4] S. Festa, B. M. Coppotelli, I. S. Morelli, “Bacterial diversity and functional interactions between bacterial strains from a phenanthrene-degrading consortium obtained from a chronically contaminated-soil”, International Biodeterioration & Biodegradation, vol. 85, pp.42-51.2013.
[5] F. Moscoso, L. Ferreira, F. J. Deive, P. Morán, M. A. Sanromán, “Viability of phenanthrene biodegradation by an isolated bacterial consortium: optimization and scale-up”, Bioprocess and Biosystems Engineering, vol. 36, no.2, pp. 133-141.2013.
[6] Z. Wang, J. Zhang, Y. Zhang, A. Hesham, M. Yang, “Molecular Characterization of a Bacterial Consortium Enriched from an Oilfield that Degrades Phenanthrene”, Biotechnology Letters, vol. 28 no.9, pp.617-621.2006.
[7] A. Pedetta, K. Pouyte, M. K. Herrera Seitz, P. A. Babay, M. Espinosa, M. Costagliola, “Phenanthrene degradation and strategies to improve its bioavailability to microorganisms isolated from brackish sediments” International Biodeterioration & Biodegradation, vol. 84, pp.161-167.2013.
[8] S. Boochan, M. L., Britz, G. A. Stanlty, “Degradation and mineralization of high-molecular-weight polycyclic aromatic hydrocarbons by defined fungal–bacteria cocultures”, Applied and Environmental Microbiology, vol. 66, no.3, pp.1007-1019. 2000.
[9] I. Avanzi, R. Gracioso, M. D. P. G. Baltazar, B. Karolski, E. A. Perpetuo, C. A. Nascimento, “Aerobic Biodegradation of Gasoline Compounds by Bacteria Isolated from a Hydrocarbon-Contaminated Soil”, Environmental Engineering Science, vol. 32, no.12, pp. 990-997. 2015.