Role of Inflammatory Markers in Arthritic Rats Treated with Ethanolic Bark Extract of Albizia procera
Rheumatoid arthritis (RA) is a chronic, progressive, systemic inflammatory disorder affecting the synovial joints and typically producing symmetrical arthritis that leads to joint destruction, which is responsible for the deformity and disability. Despite improvements in the treatment of RA over the past decade, there still is a need for new therapeutic agents that are efficacious, less expensive, and free of severe adverse reactions. The present study aimed to investigate role of inflammatory markers in arthritic rats treated with ethanolic bark extract of Albizia procera. The protective effect of ethanolic bark extract of Albizia procera against complete Freund’s adjuvant (CFA) induced arthritis in rats. Arthritis was induced by an intradermal injection of 0.1 ml FCA in the foot pad of left hind limb of rats. ETBE (100 and 200 mg/kg b.wt./p.o) and the reference drug diclofenac (25 mg/kg b.wt./p.o) were administered to arthritic rats. Paw volume was measured for all the animals before inducing arthritis and thereafter once in seven days by using plethysmometer for 42 days. Gene expression of inflammatory markers such as IL-1β and IL-10 were investigated in paw tissues. Up regulation of IL-1β and Down regulation IL-10 were observed in CFA injected rats when compared to normal rats. ETBE attenuated these alterations dose dependently when compared to the vehicle treated rats. These results provide insights into the mechanism of anti-arthritic activity, and unravel potential therapeutic use of Albizia procera in arthritis.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1126313Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1074
 Pearson, C. M. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc Soc Exp Biol Med, 1956, 91, 95-101.
 Choy, E. H. & Panayi, G. S. 2001. Cytokine pathways and joint inflammation in rheumatoid arthritis. N Engl J Med, 344, 907-16.
 Feldman, M., Brennan, F.M. & Maini, R.N. ‘Role of cytokines in rheumatoid arthritis’, Annu Rev Immunol, 1996, 14, 397-440.
 Tripathy S, Pradhan D, Anjana M. Anti-inflammatory and antiarthritic potential of Ammania baccifera Linn. Int J Pharm Bio Sci. 2010,1:1–7.
 Shah BN, Nayak BS, Seth AK, Jalalpure SS, Patel KN, Patel MA, Search for medicinal plants as a source of anti-inflammatory and anti-arthritic agents - A review. Pharmacog Mag. 2006;2:77–86.
 Kritikar K.R. and B.D. Basu “International book distribution”, Dehradun, India, Indian medicinal plants, 1987,943.
 OECD, “Guidelines for the Testing of Chemicals / Section 4: Health Effects Test No. 423: Acute Oral toxicity - Acute Toxic Class Method”, Organization for Economic Cooperation and Development, Paris, France, 2002.
 Paran, D., Kidron, D., Mayo, A., Ziv,O., Chowers, Y., Caspi, D., Yaron,M. & Paran, H, “Somatostatin analogue treatment attenuates histological findings of inflammation and increases mRNA expression of interleukin-1 beta in the articular tissues of rats with ongoing adjuvant induced arthritis”, Rheumatol Int, 2005,25(5),350-356.
 Hall, L.R., Mehlotra, R.K., Higgins, A.W., Haxhiu, M.A & Pearlman, E, “An essential role for interleukin-5 and eosinophils in helminth-induced airway hyper responsiveness”, Infection and Immunity, 1998, 66, 4425–4430.
 Olsen, I., Bon-Gharios, S. & Abraham, D. The activation of nresting lymphocytes is accompanied by the biogenesis of lysosomal organnels. Eur J Immunol, 1990, 20, 2161-2170.
 Choi, S. S., Lee, J. K. & Suh, H. W. Anti nociceptive profiles of aspirin and acetaminophen in formalin, substance P and glutamate pain models. Brain Res, 2002,921, 233-9.
 Walz, D.T., Dimartino, M.J. & Misher, A. Adjuvant induced arthritis in rats. II. Drug effects on physiologic, biochemical and immunologic parameters. J Pharmacol Exp Ther, 1971, 178(1), 223-231.