The Correlation of Total Phenol Content with Free Radicals Scavenging Activity and Effect of Ethanol Concentration in Extraction Process of Mangosteen Rind (Garcinia mangostana)

Authors: Ririn Lestari Sri Rahayu, Mustofa Ahda

Abstract:

The use of synthetic antioxidants often causes a negative effect on health and increases the incidence of carcinogenesis. Development of the natural antioxidants should be investigated. However, natural antioxidants have a low toxicity and are safe for human consumption. Ethanol extract of mangosteen rind (Garcinia mangostana) contains natural antioxidant compounds that have various pharmacological activities. Antioxidants from the ethanol extract of mangosteen rind have free radicals scavenging activities. The scavenging activity of ethanol extract of mangosteen rind was determined by DPPH method. The phenolic compound from the ethanol extract of mangosteen rind is determined with Folin-Ciocalteu method. The results showed that the absolute ethanol extract of mangosteen rind has IC₅₀ of 40.072 ug/mL. The correlation of total phenols content with free radical scavenging activity has an equation y: 5.207x + 205.51 and determination value (R²) of 0.9329. Total phenols content from the ethanol extract of mangosteen rind has a good correlation with free radicals scavenging activity of DPPH.

Keywords: Antioxidant, Garcinia mangostana, inhibition concentration 50%, total phenolic compounds.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1125367

References:

[1] Miryanti, A., Lanny, S., Kurniawan, B., and Stephen, I., Antioxidant Extraction of Mangosteen Rind (Garcinia mangostana L.), Bandung: Universitas Katolik Parahyangan, 2012.
[2] Pasaribu, F., Sitorus, P., and Bahri, S., “Ethanol Extract Test of Mangosteen Rind (Garcinia mangostana L.) to Decrease Blood Glucose Level,” Journal of Pharmaceutics and Pharmacology, Vol.1 (1), 2012, pp 1-8.
[3] Supiyanti, W., Wulansari, E., D., and Kusmita, E., “Antioxidant Test and determination Anthocyanin Total Compound of Mangosteen Rind,” Majalah Obat Tradisional, vol. 15 (2), 2010, pp 64-70.
[4] Ho, C. K., Huang, Chen, Garcinone E., “a Xanthone Derivative, Has Potent Cytotoxic Effect Against Hepatocellular Carcinoma Cell Lines,” Planta Med, vol. 68, 2002, pp 975-979.
[5] Jung, H. A., Su, B. N. Keller, W. J. Mehta, R. G. Kinghorn, A. D., “Antioxidant Xanthones from The Pericarp of Garcinia mangostana (Mangosteen),” J Agric. Food. Chem. Vol. 54, 2006, pp 2077-2082.
[6] Weecharangsan, W., Opanasopit, P., Sukma, M., Ngawhirunpat, T., Sotanaphun, U., Siripong, P., “Antioxidative and Neuroprotective Activities of Extracts from The Fruit Hull of Mangosteen (Garcinia mangostana Linn.),” Med Princ Pract, vol. 15, 2006, pp 281-287.
[7] Shibata, M., A., Matoba, Y., Tosa H., and Iinuma, M., “Effects of Mangosteen Pericarp Extracts Against Mammary Cancer,” Altern Integ Med, vol. 2 (8), 2013, pp 1-5.
[8] Kurniawati, M., Mahdi, C., and Aulanni’am, A., “The Effect of Juice Mangosteen Rind (Garcinia Mangostana L.) to Blood Sugar Levels and Histological of Pancreatic Rats with the Induction of Streptozotocin,” J. Pure App. Chem., Res., vol. 3 (1), 2014, pp 1‐6.
[9] Chaverri, J., P., Rodríguez, N., C., Ibarra, M., O., Rojas, J., M., P., “Medicinal Properties of Mangosteen (Garcinia mangostana), Food and Chemical Toxicology,” vol. 6, 2008, pp 3227–3239
[10] Shibata, M. A., Iinuma, M., Morimoto, J., Kurose, H., Akamatsu, K., Okuno, Y., Akao, Y., and Otsuki, Y., “Alfa-Mangostin Extracted from The Pericarp of the Mangosteen (Garcinia mangostana Linn) Reduces Tumor Growth and Lymph Node Metastasis in an Immunocompetent Xenograft Model of Metastatic Mammary Cancer Carrying a P53 Mutation,” BMC Medicine, vol. 9, 2011, pp 69.
[11] Khan, A., Rahman, M., Islam, M., S., “Isolation and Bioactivity of a Xanthone Glycoside from Peperomia pellucid, Life Sciences and Medicine Research,” 2010, LSMR-1.
[12] Purwaningsih, Y., dan Ersam, T., “Xanthone as Antioxidant compound from Garcinia tetranda Pierre woods,” Akta Kimindo, vol. 2, (2), 2007, pp 103 – 108.
[13] Palakawong, C., Sophanodora, P., Pisuchpen, S. and Phongpaichit, S., “Antioxidant and Antimicrobial Activities of Crude Extracts from Mangosteen (Garcinia mangostana L.) Parts and Some Essential Oils,” International Food Research Journal, vol. 17, 2010, pp 583-589.
[14] Dyahnugra, A., A., and Widjanarko, S., B., “The Appropiation of Mengosteen Rind Extract Powder (Garcinia mangostana L.) Increasing Blood Glucose at White Rats Male Strain of Wistar (Rattus norvegicus) with Hyperglycemic Conditions,” Journal of food and agro-industry, Vol. 3 (1), 2015, pp 113-123.
[15] Bondet, V., Williams W., B., and Berset, C., “Kinetics and Mechanisms of Antioxidant Activity Using the DPPH• Free Radical Method, Lebensm.-Wiss. u.,” Technol, vol. 30, 1997, pp 609–615.