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Efficient Callus Induction and Plant Regeneration from Mature Embryo Culture of Barley (Hordeum vulgare L.) Genotypes

Authors: Münüre Tanur Erkoyuncu, Mustafa Yorgancılar


Crop improvement through genetic engineering depends on effective and reproducible plant regeneration systems. Immature embryos are the most widely used explant source for in vitro regeneration in barley (Hordeum vulgare L.). However, immature embryos require the continuous growth of donor plants and the suitable stage for their culture is also certainly limited. On the other hand, mature embryos can be procured and stored easily; they can be studied throughout the year. In this study, an effective callus induction and plant regeneration were aimed to develop from mature embryos of different barley genotypes. The effect of medium (MS1 and MS2), auxin type (2,4-D, dicamba, picloram and 2,4,5-T) and concentrations (2, 4, 6 mg/l) on callus formation and effect of cytokinin type (TDZ, BAP) and concentrations (0.2, 0.5, 1.0 mg/l) on green plant regeneration were evaluated in mature embryo culture of barley. Callus and shoot formation was successful for all genotypes. By depending on genotype, MS1 is the best medium, 4 mg/l dicamba is the best growth regulator in the callus induction and MS1 is the best medium, 1 mg/l BAP is the best growth regulator in the shoot formation were determined.

Keywords: Embryo Culture, callus, barley, mature embryo

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[1] V. K. Sharma, R. Hansch, R. R. Mendel, J. Schulze, “Mature Embryo Axis-Based High Frequency Somatic Embryogenesis and Plant Regeneration from Multiple Cultivars of Barley (Hordeum vulgare L.)”, Journal of Experimental Botany, doi:10.1093/jxb/eri186, pp. 1913-1922, 2005.
[2] A. Tidema, E. Truve, “Efficient regeneration of fertile barley plants from callus cultures of several Nordic cultivars.” Hereditas, vol. 140, pp. 171-176, 2004.
[3] S. Ganeshan, S.V. Chodaparambil, M. Baga, D.B. Fowler, P. Hucl, B.G. Rossnagel, R.N. Chibbar, “In vitro regeneration of cereals based on multiple shoot induction from mature embryos in response to thidiazuron”, Plant Cell Tiss. Org. Cult., vol. 85, pp. 63-73, 2006.
[4] L.S. Dahleen, “Donor-plant environment effects on regeneration from barley embryo derived callus”, Crop Sci., vol. 39, pp. 682-685, 1999.
[5] T. Bartok, F. Sagi, “A new endosperm supported callus induction method for wheat (T. aestivum)”, Plant Cell Tissue Organ Cult., vol. 21, pp. 37-41, 1990.
[6] T. He, J.F. Jia, “High frequency plant regeneration from mature embryo explants of highland barley (Hordeum vulgare L. var. nudum Hk. f.) under endosperm-supported culture”, Plant Cell Tiss Org Cult., vol. 95(2), pp. 251–254, 2008.
[7] T. Murashige, F. Skoog, “A revised medium for rapid growth and bioassays with tobacco tissue cultures”, Physiol. Plant, vol. 15, pp. 473-497, 1962.
[8] Y. Han, X. Jın, F. Wu, G. Zhang, 2011,” Genotypic Differences in Callus Induction and Plant Regeneration from Mature Embryos of Barley (Hordeum vulgare L.)”, Journal of Zhejiang University-Scıence, vol. 12(5), pp. 399-407, May 2011.
[9] A. M. Castillo, B. Egaña, J. M. Sanz, L. Cistué, “Somatic Embryogenesis and Plant Regeneration from Barley Cultivars Grown in Spain”, Plant Cell Reports, vol. 17, pp. 902-906, 1998.
[10] E. Halámková, J. Vagera, L. Ohnoutková, “Regeneration capacity of calli derived from immature embryos in spring barley cultivars”, Biol. Plant., vol. 48, pp. 313-316, 2004.
[11] V. Serhantova, J. Ehrenbergerova, L. Ohnoutkova, “Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic”, Plant Soil Environ, vol. 50, pp. 456-462, 2004.
[12] M. Özgen, M.A. Birsin, S. Önde, “The effect of hybrid vigor on callus induction and plant regeneration from mature embryo culture of barley (Hordeum vulgare)”, Plant Cell Tiss Org Cult., vol. 82(1), pp. 67–74, 2005.
[13] K. Haliloğlu, P. S. Baenzinger, “Screening wheat genotypes for high callus induction and regeneration capability from immature embryos cultures”, J. Plant Biochemistry and Biotechnology, vol. 14, pp. 77–82, 2005.
[14] L.S. Dahleen, “Improved plant regeneration from barley callus cultures by increased copper levels”, Plant Cell Tiss. Org. Cult., vol. 43, pp. 267-269, 1995.
[15] A. Przetakiewicz, W. Orczyk, A. Nadolska-Orczyk, “The effect of auxin on plant regeneration of wheat, barley and triticale”. Plant Cell Tiss. Org. Cult., vol. 73, pp. 245-256, 2003.
[16] J.C. Popelka, F. Altpeter, “Interactions between genotypes and culture media components for improved in vitro response of rye (Secale cereale L.) inbred lines”, Plant Cell Reports, vol. 20, pp. 575–582, 2001.
[17] J.M. Zapata, B. Sabater, M. Martin, “Callus induction and in vitro regeneration from barley mature embryos”. Biol Plant., vol. 48(3), pp. 473–476, 2004.
[18] Z. Vitanova, V. Vitanov, A. Trifonova, D. Savova, A. Atanassov, “Effect of 2,4-D precultivation on regeneration capacity of cultivated barley”, Plant Cell Rep., vol. 14, pp. 437–441, 1995.
[19] A. Gürel, M. Tosun, İ. Demir, “Bazı Makarnalık ve Ekmeklik Buğday Genotiplerinin Anter Kültürüne Reaksiyonları”, J. of AARI., vol. 2, pp. 98-111, 1993.
[20] J. Ren, X. Wang, J. Yın, “Dicamba and sugar effect induction and plant rejeneration from mature embryo culture of wheat”, Agricultural sciences in china, vol. 9(1), pp. 31-37, 2010.
[21] P. Bregitzer, L.S. Dahleen, R.D. Campbell, “Enhancement of plant regeneration from callus of commercial barley cultivars”, Plant Cell Rep., vol. 17(12), pp. 941–945, 1998.
[22] M. G. Mendoza, H. F. Kaeppler, “Auxin and sugar effects on callus induction and plant regeneration frequencies from mature embryos of wheat (Triticum aestivum L.)”, In Vitro Cellular Developmental Biology, vol. 38, pp. 39-45, 2002.
[23] B. Çabuk, “Mısırda (Zea mays L.) farklı 2,4-D dozlarının kallus oluşumu ve kromozomal yapıya etkisi”, Ankara University, Biotechnology Institute (Master thesis), 153 page, Ankara, 2010.
[24] H. Ahmet, M. Adak, “Irak’ta yetiştirilen bazı ekmeklil buğday çeşitlerinde kallus oluşumu ve bitki rejenerasyonu”, Tarım Bilimler Dergisi, vol. 133, pp. 285-292, 2007.
[25] X. Y. Shan, D. S. Li, R. D. Qu, “Thidiazuron promotes in vitro regeneration of wheat and barley”, In vitro Cell. Dev. Biol.-Plant, vol. 36, pp. 207-210, 2010.
[26] M. Gubisova, D. Mıhalık, J. Gubıs, “Optimization of Barley Mature Embryo Regeneration and Comparıson with Immature Embryos of Local Cultıvars”, Nova Biotechnologica et Chimica, vol. 11(1), pp. 57-62, 2012.