Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30135
Blood Lipid Profile and Liver Lipid Peroxidation in Normal Rat Fed with Different Concentrations of Acacia senegal and Acacia seyal

Authors: Eqbal M. A. Dauqan, A. Aminah

Abstract:

The aim of the present study was to evaluate the blood lipid profile and liver lipid peroxidation in normal rat fed with different concentrations of Acacia senegal and Acacia seyal. Thirty six Sprague Dawley male rats each weighing between 180-200g were randomly divided into two groups. Each group contains eighteen rats and were divided into three groups of 6 rats per group. The rats were fed ad libitum with commercial rat’s feed and tap water containing different concentrations of Acacia senegal and Acacia seyal (3% and 6%) for 4 weeks. The results at 4 weeks showed that there was no significant difference (p≤0.05) in the total cholesterol (TC) and triglycerides (TG) between the control group and treated groups while the results for the high density lipoprotein (HDL-C) showed a significant decrease (P≥0.05) at the 3% and 6% of gum arabic treated groups compared to control group. There was a significant increase (P≥0.05) in low density lipoprotein (LDL-C) with 3% and 6% of gum Arabic (GA) groups compared to the control group. The study indicated that there was no significant (p≤0.05) effect on TC and TG but there was significant effect (P≥0.05) on HDL-C and LDL-C in blood lipid profile of normal rat. The results showed that after 4 weeks of treatment the malondialdehyde (MDA) value in rat fed with 6% of A. seyal group was significantly higher (P≥0.05) than control or other treated groups of A. seyal and A. senegal studied. Thus, the two species of gum arabic did not have beneficial effect on blood lipid profile and lipid peroxidation.

Keywords: Acacia senegal, Acacia seyal, lipid profile, lipid peroxidation, malondialdehyde (MDA).

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1124067

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 983

References:


[1] Agrigum. Arabic gum. Agrigum International Limited. 2011 http://www.agrigum.com/docs/Applications_Brochure.pdf
[2] Badreldin, H.A., Amal, Z., and Gerald, B. Biological effects of gum arabic: A review of some recent research. Food and Chemical Toxicology, 2008, vol. 47: pp.1–8.
[3] Abdul-Hadi, A.H., Mahmoud, A.E., and Abdel-Wahab, H. M. Effect of gum grabic on coagulation system of albino rats. International Journal of Pharm Tech Research.2010. vol. 3, pp. 1762-1766.
[4] Wyasu, G. and Okereke, N.Z.J. Improving the film forming ability of gum Arabic. J. Nat. Prod. Plant Resour2012. vol. 2, pp. 314-317
[5] Lelon, J.K., Jumba, I.O., Keter, J.K., Wekesa, C., and Oduor, F.D.O. Assessment of physical properties of gum arabic from Acacia senegal varieties in Baringo District, Kenya. African Journal of Plant Science 2010. Vol.4, pp.95 98. http://www.academicjournals.org/AJPS/PDF/ Pdf2010/April/Lelon%20et%20al.pdf
[6] Abd-Razig, N.M., Sabahelkhier, M.K., and Idris, O.F. Effect of Gum Arabic (Acacia senegal, L. Willd) on lipid profile and performance of Laying Hens. Journal of Applied Biosciences 2010. Vol. 32, pp. 2002 – 2007.http://www.m.elewa.org/JABS/2010/32/7.pdf
[7] Mariana, A.M., María, L.B., Lorena, V., and Claudio, D.B. Gum Arabic: More Than an Edible Emulsifier. Products and Applications of Biopolymers. 2012. ISBN: 978-953-51-0226-7. DOI: 10.5772/33783
[8] Verbeken, D, S., Dierckx, and Dewettinck K. Exudate gums: occurrence, production, and applications. Appl Microbiol Biotechnol, 2003. vol. pp. 63: 10–21.DOI 10.1007/s00253-003-1354-z
[9] Eqbal, D., Halimah, A.S., Aminah, A., and Zalifah M.K. Effect of Different Vegetable Oils (Red Palm Olein, Palm Olein, Corn Oil and Coconut Oil) on Lipid Profile in Rat. Food and Nutrition Sciences 2011. vol. 2, pp. 253-258.
[10] Hatice, G., C. Cem, K. Murat, K. Muge, C. Asuman, and E. Atilla. Peroxidase activity and lipid peroxidation in strawberry (FragariaX ananassa) plants under low temperature. J. Biol. Environ. Sci. 2008.vol. 2, pp. 95-100.
[11] Eqbal, M.A.D., Aminah, A., and Halimah, A.S. Lipid profile and antioxidant enzymes in normal and stressed rat fed with palm olein. American Journal of Applied Sciences 2012. vol. 7, pp. 1071-1078.
[12] Siti Khadijah, A., Ima, N.S., Nor, Aini, U., Norhayati, M., Norazlina, M., and Kamsiah, J. Effects of repeatedly heated palm oil on serum lipid profile, lipid peroxidation and homocysteine levels in a Post-Menopausal rat model. McGill Journal of Medicine 2008. vol. 2, pp 145-151
[13] Niedworok, E., and Bielaszka, A. Comparison of the effect of vitamins A and E on aging processes of edible vegetable oils. Polish J. of Environ. Stud 2007. vol. 6, pp. 861-865.
[14] Jarmila, Z., Jiri, S., Katerina, K., Anna, K., Tomas, H., Vaclav, Z., and Alena, F. Effects of oxidiseddietary cod liver oil on the reproductive functions of wistar rat. Czech J. Food Sci. 2004.vol. 3, pp. 108–120.
[15] Fakher, S.H., Djalali, M., Tabei, S.M.B., Zeraati, H., Javadi, E., Sadeghi, M.R., Mostafavi, E., Fatehi, F. Effect of vitamins A, E, C and omega-3 fatty acids on lipid peroxidation in streptozotocininduced diabetic rat, Iranian J PublHealt. 2007. vol. 2, pp. 58-63
[16] Mohammad, N., Aliyeh, G., Laya, F., Vahideh, S., and Marefat, G.N. Vitamins C, E and lipid peroxidation levels in sperm and seminal plasma of asthenoteratozoospermic and normozoospermic men. Iranian Journal of Reproductive Medicine. 2008.Vol. 1, pp. 1-5http://www.bioline.org.br/pdf?rm08001
[17] Puavilai, W., and Laoragpongse, D. Is Calculated LDL-C by using the new modified Friedewald equation better than the standard Friedewald equation? J. Med. Assoc. Thai., 2004. Vol. 87, pp. 589-593
[18] Oxford biomedical research. Colorimetric assay for lipid peroxidation. 2003, pp. 1-7.
[19] Ohkawa, H., Nobuko, O., and Kunio, Y. Assay for lipid peroxides in animal tissues acid reaction. Anal.Biochem1979. vol. 95, pp. 351-358.
[20] Ali, B. H., Alqarawi, A.A., and Ahmed, I. H. Does treatment with gum Arabic affect experimental chronic renal failure in rats? Blackwell Publishing Fundamental & Clinical. Doi: 10.1111/j.1472-8206.2004.00241.x Pharmacology 2004.Vol. 18, pp. 327–329
[21] Samia, T., kamal, F.E., and Khadija, A. The effect of gum Arabic on body weight and some blood elements in New Zeland cross California and baladi rabbits. Pakistan journal of biological sciences. 2006. vol. 1, pp. 96-98
[22] James, J.K., andAlan, C.T. Effect of pectin, gum arabic and agar on cholesterol absorption, Synthesis, and turnover in rats. The journal of nutrition. 1978. pp. 630-639 .http://jn.nutrition.org/content/108/4/630.full.pdf
[23] Donna, Z.B.T., Peter, S. Charles, R.S., and Gregg, S.R. Supplementation with gum arabic fiber increases fecal nitrogen excretion and lowers serum urea nitrogen concentration in chronic renal failure patients consuming a low-protein diet. Am J C/in Nuir. 1996. Vol. 63, pp. 392-8http://www.fcf.usp.br/Ensino/Graduacao/Disciplinas/Exclusivo/Inserir/Anexos/LinkAnexos/grupo%2018%20artigo%20a.pdf
[24] Badreldin, H.A., Amal, Z., and Gerald, B. Biological effects of gum arabic: A review of some recent research. Food and Chemical Toxicology. 2009. vol. 47, pp. 1–8. http://faculty.ksu.edu.sa/18856/ Articles/Biological%20effects%20of%20gum%20arabic%20A%20review%20of%20some%20recent%20research.pdf
[25] Eva, N.J., Tine, B., Gitte, R., and Lars, O.D. Mini-review: The effects of apples on plasma cholesterol levels and cardiovascular risk – a review of the evidence. Journal of Horticultural Science & Biotechnology. ISAFRUIT Special Issue. 2009. pp. 34–41. http://www.jhortscib.com/isafruit/isa_pp034_041.pdf
[26] David, A.G. The Effects of dietary supplementation with gum arabic on blood pressure and renal function in subjects with type 2 diabetes mellitus. Cardiff University. 2012. pp. 1-180.
[27] Geoffry, A., Rodney, P.T., and David, L.T. Feeding Australian Acacia Gums and Gum Arabic Leads to Non-Starch Polysaccharide Accumulation in the Cecum of Rats. The journal of nutrition. 1995. pp. 283-292.
[28] Mee, K.A., and Gee, D.L. Apple fiber and gum Arabic lowers total and low-densit ylipoprotein cholesterol levels in men with mild hypercholesterolemia. Journal of American Dietetic Association. 1997. Vol. 4, pp. 422-424
[29] Smitha, M.K. Effect of spirulina on lipid profile of hyperlipidemics. University of Agricultural sciences. 2006. pp. 1-76.
[30] Gallaher, D.D., Hassel, C.A., and Lee, K.J. Relationships between viscosity of hydroxypropyl methylcellulose and plasma cholesterol in hamsters. J. Nutr. 1993. 123, 1993. 732–738.
[31] Superko, H.R., Haskell, W.L., Sawrey-Kubicek, L., and Farquhar, J.W. Effects of solidand liquid guar gum on plasma cholesterol and triglyceride concentrations in moderate hypercholesterolemia. Am. J. Cardiol. 1988. vol. 62, pp. 51–55
[32] Topping, D., Illman, R.J., and Trimble, R.P. Volatile fatty acid concentrations in rats fed diets containing gum Arabic and cellulose separately and a mixture. Nutr. Re. Int. 1985. vol. 32, pp. 809–814
[33] Evans, A.J., Hood, R.L., Oaken full, D.G., and Sidhu, G.S. Relationship between structure and function of dietary fibre: a comparative study of the effects of three galactomannans on cholesterol metabolism in the rat. Br. J. Nutr. 1992. Vol. 68, pp. 217–229.
[34] Michael, H.D., Lynn, D.D., Jill, S., Mary, R.D., Kevin, C. M, Frances, C., Richard, C., Max, M., and Kenneth, H. A Low-Viscosity Soluble-Fiber Fruit Juice Supplement Fails to Lower Cholesterol in Hypercholesterolemic Men and Women. The journal of nutrition. 1998. pp. 1927-1932.
[35] Ross, A.H., Eastwood, M.A., Brydon, W.G., Anderson, J.R., Anderson, D.M. A study of the effects of dietary gum arabic in humans. Am. J. Clin. Nutr. 1983. vol. 37, pp. 368–375
[36] Sharma, R.D., Hypocholesterolaemic effect of gum acacia in men. Nutr. Res. 1985. Pp. 1321–1326
[37] Emulsifier. Products and Applications of Biopolymers. 2012. ISBN: 978-953-51-0226-7. DOI: 10.5772/33783
[38] Al-Majed, A. A., Mostafa, A.M., Al-Rikabi, A.C., and Al-Shabanah, O.A. Protective effects of oral arabic gum administration ongentamicin-induced nephrotoxicity in rats. Pharmacol Res. 2002. vol. 5, pp. 445- 51
[39] Ali, B.H., Al-Qarawi, A.A., Haroun, E.M., and Mousa, H.M. The effect of treatment with gum Arabic on gentamicinnephrotoxicity in rats: a preliminary study. Ren Fail. 2003. Vol. 1, pp. 15-20
[40] David, K., Shirley, A. Tepper, S. K., Czarnecki & Kalyana, S. Red palm oil in experimental atherosclerosis. Asia Pacific J Clin Nutr 11(Suppl): 2002. S433–S437
[41] Al-Assaf, S.A., Phillips, G.O., and Williams, P. A. Studies on acacia exudate gums. Part I: the molecular weight of Acacia senegal gum exudate. FoodHydrocolloids1. 2005. vol. 4, pp. 647-660.