Effect of Capsule Storage on Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Yogurt Powder
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Effect of Capsule Storage on Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Yogurt Powder

Authors: Kanchana Sitlaothaworn


Yogurt capsule was made by mixing 14% w/v of reconstitution of skim milk with 2% FOS. The mixture was fermented by commercial yogurt starter comprising Lactobacillus bulgaricus and Streptococcus thermophilus. These yogurts were made as yogurt powder by freeze-dried. Yogurt powder was put into capsule then stored for 28 days at 4oc. 8ml of commercial yogurt was found to be the most suitable inoculum size in yogurt production. After freeze-dried, the viability of L. bulgaricus and S. thermophilus reduced from 109 to 107 cfu/g. The precence of sucrose cannot help to protect cell from ice crystal formation in freeze-dried process, high (20%) sucrose reduced L. bulgaricus and S. thermophilus growth during fermentation of yogurt. The addition of FOS had reduced slowly the viability of both L. bulgaricus and S. thermophilus similar to control (without FOS) during 28 days of capsule storage. The viable cell exhibited satisfactory viability level in capsule storage (6.7x106cfu/g) during 21 days at 4oC.

Keywords: Yogurt capsule, Lactobacillus bulgaricus, Streptococcus thermophilus, freeze-drying, sucrose.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1339155

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[1] C. J. Ziemer, and G. R. Gibson, “An overview of probiotics, prebiotics and synbiotics in the functional food concept:Perspectives and future strategies,” Int. Dairy Journal., vol. 8, 1998, pp. 473-479.
[2] M. B. Roberfroid, “Prebiotics and probiotics: Are they functional foods?,” AM. J. CLIN. NUTR., vol. 71, Apr. 2000, pp. 1682-1687.
[3] N. P. Shah, “Bifidobacterium spp: Applications in fermented milks,” In Encyclopaedia of Dairy Science., May. 2002, pp. 147-151.
[4] P. capela, T. K. C. Hay, and N. p Shah, “Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yougurt and freeze-dried yogurt,” Food Research International., vol. 39, Jul. 2006, pp. 203-211.
[5] R. Fuller, “Probiotics in man and animals,” J. Appl. Bacteriol,, vol. 66, Jan. 1989, pp. 365–378.
[6] R. Fry, “CRYONICE,” (Book style), 3RDQuarter, 2007, vol. 28, pp. 3-5.
[7] S. Rybka, K. Kailasapathy, “The survival of culture bacteria in fresh and freeze-dried AB yoghurt,” Aust. J. Dairy. Tech., vol. 50, 1995, pp. 51- 57.