Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 30517
Surface Characteristics of Bacillus megaterium and Its Adsorption Behavior onto Dolomite

Authors: Mohsen Farahat, Tsuyoshi Hirajima

Abstract:

Surface characteristics of Bacillus megaterium strain were investigated; zeta potential, FTIR and contact angle were measured. Surface energy components including Lifshitz-van der Waals, Hamaker constant, and acid/base components (Lewis acid/Lewis base) were calculated from the contact angle data. The results showed that the microbial cells were negatively charged over all pH regions with high values at alkaline region. A hydrophilic nature for the strain was confirmed by contact angle and free energy of adhesion between microbial cells. Adsorption affinity of the strain toward dolomite was studied at different pH values. The results showed that the cells had a high affinity to dolomite at acid pH comparing to neutral and alkaline pH. Extended DLVO theory was applied to calculate interaction energy between B. megaterium cells and dolomite particles. The adsorption results were in agreement with the results of Extended DLVO approach. Surface changes occurred on dolomite surface after the bio-treatment were monitored; contact angle decreased from 69° to 38° and the mineral’s floatability decreased from 95% to 25% after the treatment.

Keywords: Surface modification, Flotation, Bacillus megaterium, dolomite, adhesion energy

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1327246

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1617

References:


[1] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Yuuki Aiba, Katsumi Doi, Adsorption of SIP E. coli onto quartz and its applications in froth flotation, Minerals Engineering, Volume 21, Issue 5, April 2008, Pages 389–395.
[2] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Katsumi Doi, Adhesion of Escherichia coli onto quartz, hematite and corundum: extended DLVO theory and flotation behavior, Colloids and Surfaces B: Biointerfaces, Volume 74, Issue 1, 1 November 2009, Pages 140-149.
[3] Huifen Yang, Qiongyao Tang, Chuanlong Wang, Jinlong Zhang, Flocculation and flotation response of Rhodococcus erythropolis to pure minerals in hematite ores, Minerals Engineering, Volume 45, May 2013, Pages 67–72.
[4] Leslie Y. Lopez, Antonio G. Merma, Mauricio L. Torem, Gabriela H. Pino, Fundamental aspects of hematite flotation using the bacterial strain Rhodococcus ruber as bioreagent, Minerals Engineering, Volume 75, 1 May 2015, Pages 63–69.
[5] Partha Patra, K.A. Natarajan, Surface chemical studies on selective separation of pyrite and galena in the presence of bacterial cells and metabolic products of Paenibacillus polymyxa, Journal of Colloid and Interface Science, Volume 298, Issue 2, 15 June 2006, Pages 720–729.
[6] M.N Chandraprabha, K.A Natarajan, P Somasundaran, Selective separation of arsenopyrite from pyrite by biomodulation in the presence of Acidithiobacillus ferrooxidans, Journal of Colloid and Interface Science, Volume 276, Issue 2, 15 August 2004, Pages 323–332.
[7] Mohsen Farahat, Tsuyoshi Hirajima, Keiko Sasaki, Adhesion of Ferroplasma acidiphilum onto pyrite calculated from the extended DLVO theory using the van Oss–Good–Chaudhury approach, Journal of Colloid and Interface Science, Volume 349, Issue 2, 15 September 2010, Pages 594–601.
[8] Mohsen Farahat, Tsuyoshi Hirajima, Hydrophilicity of Ferroplasma acidiphilum and its effect on the depression of pyrite, Minerals Engineering, Volumes 36–38, October 2012, Pages 242–247.
[9] Tsuyoshi Hirajima, Yuki Aiba, Mohsen Farahat, Naoko Okibe, Keiko Sasaki, Takehiko Tsuruta, Katsumi Doi, Effect of microorganisms on flocculation of quartz, International Journal of Mineral Processing, Volumes 102–103, 25 January 2012, Pages 107–111.
[10] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A., Surface chemical characterization of Paenibacillus polymyxa before and after adaptation to sulfide minerals, International journal of Mineral Processing, 62 (2001) 3-25.
[11] Rijnaarts, H.M., Norde, W, Lyklema, J., Zehnder, A, The isoelectric point of bacteria as an indicator for the presence of cell surface polymers that inhibit adhesion. Colloids and Surfaces B: Biointerfaces, 4 (1995) 191-197.
[12] Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E., Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions, Hydrometallurgy, 71 (2003) 285–292.
[13] Van der Mei, H. C.; Bos, R.; Busscher, H. J, A reference guide to microbial cell surface hydrophobicity based on contact angles, Colloids and Surfaces B: Biointerfaces, 11 (1998) 213-221.
[14] Patricia S. Vary, Rebekka Biedendieck, Tobias Fuerch, Friedhelm Meinhardt, Manfred Rohd, Wolf-Dieter, Deckwer, Dieter Jahn, Bacillus megaterium—from simple soil bacterium to industrial protein production host, Appl Microbiol Biotechnol (2007) 76:957–967.
[15] Beveridge TJ., Ultrastructure, chemistry, and function of the bacterial wall. International Review of Cytology, 1981;72:229-317.
[16] Wei Jiang, Anuradha Saxena, Bongkeun Song, Bess B. Ward, Terry J. Beveridge, Satish C. B. Myneni, Elucidation of Functional Groups on Gram-Positive and Gram-Negative Bacterial Surfaces Using Infrared Spectroscopy, Langmuir 2004, 20, 11433-11442.
[17] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Microbially induced selective flotation of sphalerite from galena using mineraladapted strains of Bacillus megaterium, Colloids and Surfaces B: Biointerfaces, Volume 112, 1 December 2013, Pages 279–286.
[18] B. Vasanthakumar, H. Ravishankar, S. Subramanian, Basic studies on the role of components of Bacillus megaterium as flotation biocollectors in sulphide mineral separation, Appl Microbiol Biotechnol. 2014 Mar;98(6):2719-28.
[19] Busscher, H.J., Weerkamp, A. H., Van der Mei H.C., van pelt A.W.J, de jong H.P., Arends J., Measurements of surface free energy of bacterial cell surfaces and its relevance to adhesion, Applied environmental microbiology, 48(5) (1984) 980-983.
[20] Van Oss, C.J., Gillman, C.F., Neumann, A.W., 1975, Chapter 2, In: Phagocytic engulfment and cell adhesiveness, Isenberg, H.D., Eds., Dekker, New York, 13-16 Van der Mei, H.C., Bos, R., Busscher, H.J., A reference guide to microbial cell surface hydrophobicity based on contact angles, Colloid and Surfaces B: Biointerfaces, 11 (1998) 213- 221
[21] Van der Mei, H.C., Bos, R., Busscher, H.J., A reference guide to microbial cell Surface hydrophobicity based on contact angles, Colloids and surfaces B: Biointerfaces, 11(1998) 213-221.
[22] Bellamy, L.J., In Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions, Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E., Hydrometallurgy 71 (2003) 285–292.
[23] Twardowski, J., Anzenbacher, P., In Surface characterization of Acidithiobacillus ferrooxidans cells grown under different conditions, Sharmaa, P.K., Dasb, Hanumantha Raoa, A., K., Forssberg, K.S.E., Hydrometallurgy 71 (2003) 285–292.
[24] Sharma, P.K., Hanumantha Rao, K., Forssberg, K.S.E., Natarajan, K.A., Surface chemical characterization of Paenibacillus polymyxa before and after adaptation to sulfide minerals, International journal of Mineral Processing, 62 (2001) 3-25.
[25] Dudley William Thompson, Pamela Gillian Pownall, Surface electrical properties of calcite, Journal of Colloid and Interface Science, Volume 131, Issue 1, August 1989, Pages 74–82.
[26] Mélanie Davranche, Sandrine Beaufreton, Jean-Claude Bollinger, Influence of Carbonates on the Surface Charge of a Natural Solid, Journal of Colloid and Interface Science, volume 249, Issue 1, 1 May 2002, Pages 113–118.
[27] Daniel S Cicerone, Alberto E Regazzoni, Miguel A Blesa, Electrokinetic properties of the calcite/water interface in the presence of magnesium and organic matter, Journal of Colloid and Interface Science, volume 154, Issue 2, December 1992, Pages 423–433.
[28] Reda Marouf, Kheira Marouf-Khelifa, Jacques Schott, Amine Khelifa, Zeta potential study of thermally treated dolomite samples in electrolyte solutions, Microporous and Mesoporous Materials 122 (2009) 99–104.
[29] Van Oss, C. J. Hydrophobicity of biosurfaces-Origin, quantitative determination and interaction energies, Colloids and Surfaces B: Biointerfaces, 5 (1995) 91-110.
[30] Van Oss, C.J., Interfacial forces in aqueous media, Marcel Dekker, New York, (1994).
[31] Brown, Derick G., Effects of Nonionic Surfactants on the Cell Surface Hydrophobicity and Apparent Hamaker Constant of a Sphingomonas sp, Environmental Science and Technology, 40 (1) (2006) 195 -201.
[32] Vernhet, A., Bellon-Fontaine M.N., Role of bentonites in the prevention of Saccharomyces cerevisiae adhesion to solid surfaces, Colloids and Surfaces B: Biointerfaces 3 (I995) 255-262.
[33] Dopson, M., E.B. Lindstrom, Potential role of Thiobacillus caldus in arsenopyrite bioleaching, Applied Environmental Microbiology, 65 (1999) 36-40.