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Assessment of Landfill Pollution Load on Hydroecosystem by Use of Heavy Metal Bioaccumulation Data in Fish

Authors: Gintarė Sauliutė, Gintaras Svecevičius


Landfill leachates contain a number of persistent pollutants, including heavy metals. They have the ability to spread in ecosystems and accumulate in fish which most of them are classified as top-consumers of trophic chains. Fish are freely swimming organisms; but perhaps, due to their species-specific ecological and behavioral properties, they often prefer the most suitable biotopes and therefore, did not avoid harmful substances or environments. That is why it is necessary to evaluate the persistent pollutant dispersion in hydroecosystem using fish tissue metal concentration. In hydroecosystems of hybrid type (e.g. river-pond-river) the distance from the pollution source could be a perfect indicator of such a kind of metal distribution. The studies were carried out in the Kairiai landfill neighboring hybrid-type ecosystem which is located 5 km east of the Šiauliai City. Fish tissue (gills, liver, and muscle) metal concentration measurements were performed on two types of ecologically-different fishes according to their feeding characteristics: benthophagous (Gibel carp, roach) and predatory (Northern pike, perch). A number of mathematical models (linear, non-linear, using log and other transformations) have been applied in order to identify the most satisfactorily description of the interdependence between fish tissue metal concentration and the distance from the pollution source. However, the only one log-multiple regression model revealed the pattern that the distance from the pollution source is closely and positively correlated with metal concentration in all predatory fish tissues studied (gills, liver, and muscle).

Keywords: Heavy Metals, Landfill Leachate, mathematical model, bioaccumulation in fish, hydroecosystem

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[1] R. J. Slack, J. R. Gronow, N. Voulvoulis, “Household hazardous waste in municipal landfills: contaminants in leachate“, Science of the Total Environment, 2005, 337: 119–137.
[2] S. Vasarevičius, J. Čegariova, D. Sližytė, “Investigation and evaluation of landfill leachate permeability in the soil”, Journal of Environmental Engineering and Landscape Management, 2005, XIII (3):108–115.
[3] M. V. Pablos, F. Martini, C. Fernandez, M. M. Babín, I. Herraez, J. Miranda, J. Martínez, G. Carbonell, L. San-Segundo, P. GarcíaHortigüela, J. V. Tarazona, “Correlation between physicochemical and ecotoxicological approaches to estimate landfill leachates toxicity”, Waste Management, 2011, 31: 1841–1847.
[4] J. Y. M. Alkassasbeh, L. Y. Heng, S. Surif, “Toxicity testing and the effect of landfill leachate in Malaysia on behavior of common carp (Cyprinus carpio L., 1758; Pisces, Cyprinidae)”, American Journal of Environmental Sciences, 2009, 5(3): 209–217.
[5] V. Singh, A. K. Mittal, “Toxicity analysis and public health aspects of municipal landfill leachate: A case study of Okhla landfill”, Delhi. 8th World Wide Workshop for Young Environmental Scientists WWW-YES 2009: Urban waters: resource or risks?”, Arcueil, France, 2009.
[6] A. O. Aderemi, G. A. Adewumi, A. A. Otitoloju, “Municipal landfill leachate characterization and its induction of glycogen vacuolation in the liver of Clarias gariepinus”, International Journal of Environmental Protection, 2012, 2(4): 20–24.
[7] V. Tsarpali, S. Dailianis, “Landfill leachate composition and toxic potency in semi-arid areas: an integrated approach with the use of physicochemical and toxicological data”, Third International Symposium on Green chemistry for environment, health and development, 3-5 October 2012, Skiathos Island, Greece.
[8] S. R. Qasim, W. Chiang, “Sanitary Landfill Leachate, Generation, Treatment and Control”, Technomic Publishing Co. Inc., Lancaster, Pennsylvania, 1994, 338 p.
[9] D. L. Jones, K. L. Williamson, A. G. Owen, “Phytoremediation of landfill leachate”, Waste Management, 2006, 26(8): 825–837.
[10] Anonymous, “Closed old landfills. Less contaminated areas“, Baltijos kopa, Vilnius, Lithuania, 2012, 51 p. (in Lithuanian).
[11] B. Słomczyńska, T. Słomczyński, “Physicochemical and toxicological characteristics of leachates from MSW landfills”, Polish Journal of Environmental Studies, 2004, 13(6): 627–37.
[12] C. Bernes, “Persistent Organic Pollutants. A Swedish View of an International Problem”, Swedish Environmental Protection Agency, Monitor 16. Bignert A., 1998.
[13] Directive 2008/105/EC of the European Parliament and of the Council of 496 16 December 2008 on environmental quality standards in the field of water 497 policy, amending and subsequently repealing Council Directives 82/176/EEC, 498 83/513/EEC, 84/156/EEC, 84/491/EEC, 86/280/EEC and amending Directive 499 2000/60/EC of the European Parliament and of the Council. Official Journal of the European Communities L 500 348, 24/12/2008 p. 0084-0097.
[14] US EPA. National recommended water quality criteria. Office of Water, office of Science and Technology (4304T), Washington, DC, 2009, 25, accessed 13 November 2015 via
[15] Scorecard. Water quality indicators. Ambient water quality: four toxic pollutants, 2011, accessed 9 December 2015 via
[16] M. K. Sabullah, S. A. Ahmad, M. Y. Shukor, A. J. Gansau, M. A. Syed, M. R. Sulaiman, N. A. Shamaan, “Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approaches“, International Food Research Journal, 2015, 22(2): 435–454.
[17] M. R. Lasheen, F. Kh. Abdel-Gawad, A. A. Alaneny, H. M. H. Abd El bary, ”Fish as Bio Indicators in Aquatic Environmental Pollution Assessment: A Case Study in Abu-Rawash Area, Egypt“, World Applied Sciences Journal, 2012, 19(2): 265–275.
[18] N. R. Bury, P. A. Walker, C. N. Glover, “Nutritive metal uptake inteleost fish”, Journal of Experimental Biology, 2003, 206: 11–23.
[19] A. Chovanec, R. Hofer, F. Schiemer, “Fishes as bioindicators. In: Markert BA, Breure AM and Zechmeister HG (eds) Bioindicators and biomonitors, principles, concepts and applications”, Amsterdam, Elsevier, 2003, pp 639–676.
[20] E. Has-Schön, I. Bogut, I. Strelec, “Heavy metal profile in five fish species included in human diet, domiciled in the end flow of river Neretva (Croatia)”, Archives of Environmental Contamination and Toxicology, 2006, 50: 545–551.
[21] B. Jezierska, M. Witeska, “The metal uptake and accumulation in fish living in polluted waters. In: Twardowska I et al. (eds) Soil and Water pollution Monitoring”, Protection and Remediation, Springer, 2006, pp. 3–23.
[22] L. Galhardo, R. F. Oliveira, “Psychological Stress and Welfare in Fish”, ARBS Annual Review of Biomedical Sciences, 2009, 11: 1–20.
[23] J. A. Hansen, D. F. Woodward, E. E. Little EE, A. J. DeLonay, H. L. Bergman, ”Behavioral avoidance: A possible mechanism for explaining abundance and distribution of trout species in a metals impacted river”, Environmental Toxicology and Chemistry, 1999, 18: 313–317.
[24] A, Valavanidis, T. Vlachogianni, “Metal pollution in ecosystems: ecotoxicology studies and risk assessment in the marine environment”, Science advances on Environment, Toxicology & Ecotoxicology issues, 17 February 2010, www.chem-tox-ecotox.
[25] S. Eastwood, P. Couture, “Seasonal variations in condition and liver metal concentrations of yellow perch (Perca flavescens) from a metal-contaminated environment”, Aquatic Toxicology, 2002, 58: 43–56.
[26] L. Bervoets, R. Blust, “Metal concentrations in water, sediment and gudgeon (Gobio gobio) from a pollution gradient: relationship with fish condition factor“, Environmental Pollution, 2003, 126: 9–19.
[27] P. D. Couture, J. W. Rajotte, “Morphometric and metabolic indicators of metal stress in wild yellow perch (Perca flavescens) from Sudbury, Ontario: A review“, Journal of Environmental Monitoring, 2003, 5: 216–221.
[28] G. G. Pyle, J. W. Rajotte, P. Couture, “Effects of industrial metals on wild fish populations along a metal contamination gradient“, Ecotoxicology and Environmental Safety, 2005, 61: 287–312.
[29] L. Bervoets, D. Knapen, M. De Jonge, K. Van Campenhout, R. Blust, “Differential hepatic metal and metallothionein levels in three feral fish species along a metal pollution gradient“, PLOS ONE, 2013, 8(3): e60805, doi:10.1371/journal.pone.0060805.
[30] G. Svecevičius, N. Kazlauskienė, V. Kesminas, R. Staponkus, E. Taujanskis, G. Sauliutė, “Heavy Metal Accumulation in Fishes of Different Ecological Groups from Kairiai Landfill Regional Aquatic Ecosystem“, 9th International Conference on Environmental Engineering, 2014, doi: 10.3846/enviro.2014.060.
[31] G. Svecevičius, N. Kazlauskienė, A. Slučkaitė, T. Makaras, “Toxicological assessment of the effects of closed landfill on neighbouring hydroecosystem”, Fresenius Environmental Bulletin, 2014, vol. 23, No 11a, p. 2926–2932.
[32] ISO 11047:2004 (2004a) Soil quality – Determination of cadmium, chromium, cobalt, copper, lead, manganese, nickel and zinc in aqua regia extracts of soil – Flame and electrothermal atomic absorption spectrophotometric methods (identical to ISO 11047:1998) ISO, the International Organization for Standardization.
[33] ISO 16772:2004 (2004b) Soil quality – Determination of mercury in aqua regia soil extracts with cold-vapour atomic spectrometry or cold–vapour atomic fluorescence spectrometry. ISO, the International Organization for Standardization.
[34] K. Benoit, “Linear Regression Models with Logarithmic Transformations“, Methodology Institute. London School of Economics, 2011.
[35] K. P. Burnham, D. R. Anderson, “Model selection and multimodel inference: a practical information-theoretic approach”, 2nd edn. Springer, New York, 2002.
[36] J. Pinheiro, D. Bates, S. DebRoy, D. Sarkar, R Core Team, “nlme: Linear and nonlinear mixed effects models”, 2014, R package version 3.1–118, accessed 19 November 2015 via
[37] M. Canli, G. Atli, “The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species”, Environmental Pollution, 2003, vol. 121, no. 1, pp. 129–136.
[38] V. Yancheva, I. Velcheva, S. Stoyanova, E. Georgieva, “Fish in Ecotoxicological Studies”, Ecologia Balkanica, 2015, Vol. 7, pp. 149–169.
[39] D. H. Evans, P. M. Piermarinis, K. P. Choe, “The mulitfunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste”, Physiological Reviews, 2005, 85, 97–177, 10.1152/physrev.00050.2003.
[40] D. Weihrauch, M. P. Wilkie, P. J. Walsh, “Ammonia and urea transporters in gills of fish and aquatic crustaceans”, Journal of Experimental Biology, 2009, 212, 1716–1730, 10.1242/jeb.024851.
[41] N. R. Bury, S. Schnell, C. Hogstrand, “Gill cell culture systems as models for aquatic environmental monitoring”, The Journal of Experimental Biology, 2014, 217, 639–650, doi:10.1242/jeb.095430.
[42] R. C. Playle, D. G. Dixon, K. Burnison, “Copper and cadmium binding to fish gills: modification by dissolved organic carbon and synthetic ligands”, Canadian Journal of Fisheries and Aquatic Sciences, 2011, 50 (12): 2667–2677.
[43] M. M. N. Authman, M. S. Zaki, E. A. Khallaf, H. H. Abbas, “Use of Fish as Bio-indicator of the Effects of Heavy Metals Pollution”, Aquaculture Research & Developmen, 2015, 6:4, accessed 26 November 2015 via
[44] G. K. Bielmyer, B. J. Bullington, A. C. DeCarlo, S. J. Chalk, K. Smith, “The Effects of Salinity on Acute Toxicity of Zinc to Two Euryhaline Species of Fish, Fundulus heteroclitus and Kryptolebias marmoratus”, Integrative and Comparative Biology, 2012, volume 52, number 6, pp. 753–760, doi:10.1093/icb/ics045.
[45] S. Niyogi, C. M. Wood, “Effects of chronic waterborne and dietary metal exposures on gill metal-binding: Implications for the Biotic Ligand Model”, Human and Ecological Risk Assessment, 2003, 9:813–846.
[46] S. Niyogi, G. G. Pyle, C. M. Wood, “Branchial versus intestinal zinc uptake in wild yellow perch (Perca flavescens) from reference and metal-contaminated aquatic ecosystems”, Canadian Journal of Fisheries and Aquatic Sciences, 2007, 64, 1605–1613.
[47] Canadian Council of Ministers of the Environment. Canadian Council of Resource and Environment Ministries, Appendix XVIII. Canadian Water Quality Guidelines, Winnipeg, MB, Canada, 1995.