Unraveling Biostimulation of Decolorized Mediators for Microbial Fuel Cell-Aided Textile Dye Decontamination

Authors: Pei-Lin Yueh, Bor-Yann Chen, Chuan-Chung Hsueh

Abstract:

This first-attempt study revealed that decolorized intermediates of azo dyes could act as redox mediators to assist wastewater (WW) decolorization due to enhancement of electron-transport phenomena. Electrochemical impedance spectra indicated that hydroxyl and amino-substituent(s) were functional group(s) as redox-mediator(s). As azo dyes are usually multiple benzene-rings structured, their derived decolorized intermediates are likely to play roles of electron shuttles due to lower barrier of energy gap for electron shuttling. According to cyclic voltammetric profiles, redox mediating characteristics of decolorized intermediates of azo dyes (e.g., RBu171, RR198, RR141, RBk5) were clearly disclosed. With supplementation of biodecolorized metabolites of RR141 and 198, decolorization performance of could be evidently augmented. This study also suggested the optimal modes of microbial fuel cell (MFC)-assisted WW decolorization would be plug-flow or batch mode of operation with no mix. Single chamber-MFCs would be more favourable than double chamber MFCs due to non-mixing contacting reactor scheme for operation.

Keywords: Redox mediators, dye decolorization, bioelectricity generation, microbial fuel cells.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1110658

References:

[1] X.Z. Wang, X Cheng, D. Sun, “Autocatalysis in Reactive Black 5 biodecolorization by Rhodopseudomonas palustris W1,” Appl. Microbiol. Biotechnol. Vol. 80, no. 5, pp. 907–915, October 2008.
[2] B.Y. Chen, J. Hong, I.S. Ng, Y.M. Wang, S.Q. Liu, B. Lin, C. Ni, “Deciphering simultaneous bioelectricity generation and reductive decolorization using mixed-culture microbial fuel cells in salty media,” J. Taiwan Inst. Chem. Engrs., vol. 44, no. 3m pp. 446-453, May 2013.
[3] X. Jin, G. Liu, Z. Xu, W. Tao, “Decolourisation of a Dye Industry Effluent by Aspergillus fumigatus XC6,” Appl. Microbiol. Biotechnol., vol. 14, no. 1, pp. 239-243, February 2007.
[4] H. Liu, S. Cheng, B.E. Logan, “Production of Electricity from Acetate or Butyrate Using a Single-Chamber Microbial Fuel Cell,” Environ. Sci. Technol. vol. 39, no. 2, pp. 658–662, 2005.
[5] D.R. Bond, D.E. Holmes, L.M. Tender, D.R. Lovley, “Electrode-reducing microorganisms that harvest energy from marine sediments,” Science, vol. 295, no. 5554, pp. 483–485, 18 January 2002.
[6] M.M. Zhang, W.M. Chen, B.Y. Chen, C.T. Chang, C.C. Hsueh, Y. Ding, K.L. Lin, H. Xu, “Comparative study on characteristics of azo dye decolorization by indigenous decolorizers,” Bioresour. Technol., vol. 101, no.8, pp. 2651–2656, April 2010.
[7] B.E. Logan, J.M. Regan, “Electricity-producing bacterial communities in microbial fuel cells,” Trends in Microbiol. vol. 14, no. 12, pp. 512-518, 2006.
[8] B. E. Logan, Chapt. 2 Exoelectrogens. In: Microbial Fuel Cells. Wiley-Interscience, pp.12-28, 2008.
[9] B.Y. Chen, B. Xu, P.L. Yueh, K. Han, L.J. Qin, C.C. Hsueh, “Deciphering electron-shuttling characteristics of thionine-based textile dyes in microbial fuel cells,” J. Taiwan Inst. Chem. Engrs., vol. 51, pp.63-70, June 2015.
[10] B.Y. Chen, M.M. Zhang, Y. Ding, C.T. Chang, ”Feasibility study of simultaneous bioelectricity generation and dye decolorization using naturally occurring decolorizers,” J. Taiwan Inst. Chem. Engrs, vol. 41, no. 6, pp. 682-688, November 2010.
[11] C.C. Hsueh, Y.M. Wang, B.Y. Chen, “Metabolite analysis on reductive biodegradation of reactive green 19 in Enterobacter cancerogenus bearing microbial fuel cell (MFC) and non-MFC cultures,” J. Taiwan Inst. Chem. Engrs, vol. 45, no. 2, pp. 436-443, March 2014.
[12] Levenspiel, O., Chapter 8 Potpourri of Multiple Reactions. In: Chemical Reaction Engineering. John Wiley & Sons, Inc. 3rd Ed, pp.170-206, 1999.
[13] J.C.W. Lan, K. Raman, C.M. Huang, C.M. Chang, “The impact of monochromatic blue and red LED light upon performance of photo microbial fuel cells (PMFCs) using Chlamydomonas reinhardtii transformation F5 as biocatalyst,” Biochem. Eng. J., vol. 78, no. 15, pp. 39-43, September 2013.
[14] B.Y. Chen, K.W. Lin, Y.M. Wang, C.Y. Yen, “Revealing interactive toxicity of aromatic amines to azo dye decolorizer Aeromonas hydrophila,” J. Hazard. Mater., vo. 166, no. 1, pp.187-194, July 2009.
[15] B.Y. Chen, Y.M. Wang, I-S. Ng, “Understanding interactive characteristics of bioelectricity generation and reductive decolorization using Proteus hauseri,” Bioresour. Technol., vo. 102, no. 2, pp. 1159-1165, January 2011.
[16] B.Y. Chen, Y.M. Wang, I.S. Ng, S.Q. Liu, J.Y. Hung, “Deciphering simultaneous bioelectricity generation and dye decolorization using Proteus hauseri,” J. Biosci. Bioeng., vol. 113, no.4, pp. 502-507, April 2012.
[17] B.Y. Chen, C.C. Hsueh, W.M. Chen, W.D. Li, “Exploring decolorization and halotolerance characteristics by indigenous acclimatized bacteria: Chemical structure of azo dyes and dose–response assessment,” J. Taiwan Inst. Chem. Engrs., vol. 42, no. 5, pp. 816-825, September 2010.
[18] B.Y. Chen, W.M. Chen, F.L. Wu, P.K. Chen, C.Y. Yen, “Revealing azo-dye decolorization of indigenous Aeromonas hydrophila from fountain spring in Northeast Taiwan,” J. Chin. Inst. Chem. Eng., vol. 39, no.5, pp. 495–501, September 2008.
[19] B.Y. Chen, “Understanding decolorization characteristics of reactive azo dyes by Pseudomonas luteola: toxicity and kinetics,” Proc. Biochem., vol. 38, no. 3, pp. 437-446, November 2002.
[20] B.Y. Chen, C.C. Hsueh, S.Q. Liu, J.Y. Hung, Y. Qiao, P.L. Yueh, Y.M. Wang, “Unveiling characteristics of dye-bearing microbial fuel cells for energy and materials recycling: Redox mediators,” International J. Hydrogen Energy, vol. 38, no.35, pp. 15598-15605, November 2013.
[21] B. Xu, B.Y. Chen, C.C. Hsueh, L.J. Qin, C.T. Chang, ”Deciphering characteristics of bicyclic aromatics-mediators for reductive decolorization and bioelectricity generation,” Bioresour. Technol., vol. 163, pp. 280-286, July 2014.
[22] J.I. Aihara, “Reduced HOMO-LUMO Gap as an Index of Kinetic Stability for Polycyclic Aromatic Hydrocarbons,” J. Phys. Chem. A,” vol. 103, no. 37, pp. 7487-7495, August 1999.
[23] K. Watanabe, M. Manefield, M. Lee, A. Kouzuma, “Electron shuttles in biotechnology,” Current Opinion in Biotechnol., vol. 20, no. 6, pp. 633-641, December 2009.
[24] C.C. Hsueh, B.Y. Chen, C.Y. Yen,”Understanding effects of chemical structure on azo dye decolorization characteristics by Aeromonas hydrophila,” J. Hazard. Mater., vol. 167, no. 1-3, pp. 995-1001, August 2009.
[25] Y. Qiao, C.M. Li, S.J. Bao, Q.L. Bao, 2007. “Carbon nanotube/poltaniline composite as anode material for microbial fuel cells,’ J. Power Source, vol. 170, no. 1, pp. 79-84, June 2007.