A New Method to Enhance Contrast of Electron Micrograph of Rat Tissues Sections
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A New Method to Enhance Contrast of Electron Micrograph of Rat Tissues Sections

Authors: Lise P. Labéjof, Raiza S. P. Bizerra, Galileu B. Costa, Thaísa B. dos Santos

Abstract:

This report presents an alternative technique of application of contrast agent in vivo, i.e. before sampling. By this new method the electron micrograph of tissue sections have an acceptable contrast compared to other methods and present no artifact of precipitation on sections. Another advantage is that a small amount of contrast is needed to get a good result given that most of them are expensive and extremely toxic.

Keywords: Image quality, Microscopy research, Staining technique, Ultrathin section.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1108374

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[1] R. A. Bloodgood, “The history of medical histology teaching: where have we come from and where are we going?” FASEB J., vol. 27, pp 191-1, 2013.
[2] J. A. Hightower, F.R. Boockfor, C. A.Blake, and C. F. Millette, “The Standard Medical Microscopic Anatomy Cours,” Histology Circa, The anatomical record (New Anat.), vol. 257, pp. 96–101, 1999.
[3] E. M. Slayter and H S. Slayte, “Light and Electron Microscopy”, Cambridge University Press, Cambridge, pp 100-110.
[4] R.M., Amderson and D, Walck, “Specimen Preparation for Transmission Electron Microscopy IV” in MRS Proceedings, Jun 5, 2014, pp. 480-483.
[5] M. A. Hayat, “Basic Techniques for Transmission Electron Microscopy,” 1986.
[6] J. Kuo, Electron microscopy: methods and protocols. 2 ed. Totowa, N.J.: Humana Press, 2007, pp. 608-624.
[7] I. M. Watt, “The Principles and Practice of Electron Microscopy,” Cambridge University Press, 1997.
[8] J. Fertig and H. Rose, “On the theory of image formation in the electron microscope,” Optik, vol. 54, pp.165- 174, 1979.
[9] L. E. Ross, M Dykstra, “Biological Electron Microscopy: Theory, techniques and troubleshooting” Springer, New York. , 2003.
[10] M. Nakakoshi, H. Nishioka, and E. Katayama, “New versatile staining reagents for biological transmission electron microscopy that substitute for uranyl acetate,” J Electron Microsc., vol. 60, pp. 401-407, 2011.
[11] S. W. Avery, “Routine Transmission Electron Microscopy (TEM) Staining Protocol for Tissues,” in Transmission Electron Microscopy, 2nd ed. vol. 3, J. Peters, Ed. New York: McGraw-Hill, 1964, pp. 15–64
[12] S. W. Avery, E. A. Ellis, “Methods for removing uranyl acetate precipitate from ultrathin sections,” Stain Technol., vol. 53, no. 3, pp. 137-40, 1978.
[13] E. S. Reynolds, “The use of lead citrate at high pH as an electron-opaque stain in electron microscopy,” Journal of Cellular Biology, vol. 17, pp 208-214, 1963.
[14] H. Niederstrasser, “Electron microscopy, Negative staining”, Snaggled Works, 2004 http://www.snaggledworks.com
[15] G. J. Gage, D. R. Kipke, and W. Shain, “Whole Animal Perfusion Fixation for Rodents,” Journal of Visualized Experiments (JoVE), vol 65, pp. 3564, 2012.
[16] R. Kasukurthi, M.J. Brenner, A.M. Morre, A. Moradzadeh, Z.R. Wilson, K.B. Santosa, S.E. Mackinnon, and D. A. Hunter, “Transcardial perfusion versus immersion fixation for assessment of peripheral nerve regeneration,” Journal of Neurosciences Methods, vol. 184, pp. 303-309, 2009.
[17] M. L. Watson, “Staining of Tissue Sections for Electron Microscopy with Heavy Metals,” Journal of Biophysical and Biochemical Biology, vol. 4, no. 4, pp. 475-478, 1958.
[18] R. S. P. Bizerra, G. B. Costa, L. P. Labejof, “Estudo morfológico em microscopia de luz e eletrônica da mucosa intestinal de ratos após administração de acetato de uranilo,” in Abstract Book of the 16° Seminário de Iniciação Cientifica, Universidade Estadual de Santa Cruz, Cruz , Ilhéus, Bahia, 2010, pp. 141-141.
[19] T. B. dos Santos, L. P. Labéjof, “Estudo correlativo em microscopia de luz e eletrônica do modo de acumulação do urânio no enterócito após ingestão experimental,”in Abstract Book of the 18° Seminário de Iniciação Cientifica , Universidade Estadual de Santa Cruz, Cruz , Ilhéus, Bahia, 2012, pp. 765-765.
[20] F. Paquet, “Accumulation and distribution of uranium in rats after chronic exposure by ingestion,” Health Physics, vol. 90, no. 2, pp.139- 147, 2006.
[21] I. Dublineau, “Absorption of uranium through the entire gastrointestinal tract of the rat,” Internat. J. Rad. Biol., vol. 81, no. 6, pp. 473–482, 2005.
[22] I. Dublineau, “Absorption, accumulation and biological effects of depleted uranium in Peyer’s patches of rats,” Toxicology, vol. 227, pp. 227–239, 2006.
[23] W. Rasband, “ImageJ - Image processing and Analysis in Java, National Institute of Mental Health (NIMH) of the National Institutes of Health (NIH), http://rsb.info.nih.gov/ij/.
[24] P. Bankhead. Analyzing fluorescence microscopy images with ImageJ Queen's University Belfast 2014.
[25] F. Zernike, “How I discovered phase contrast” Nobel Lecture, December 11, 1953