Antibacterial Activity of Some Medicinal Plant Extracts

Authors: Hayam M. Ibrahim, Ferial M. Abu-Salem

Abstract:

Medicinal plants are now gaining attractiveness in treatment of bacterial infections and food preservation. The objective of this study was to assess antibacterial activity of some medicinal plants on pathogenic bacteria. Screening of antibacterial activity of aqueous and methanol extracts of some plants: Jojoba, Ginger, Sage, Thyme and Clove against Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Clostridium perfringens and Escherichia coli were investigated. Antibacterial activity was performed by agar diffusion and disc diffusion method. Jatropha, Jojoba, Clove and Ginger extracts showed notable bacterial activity in the first screening step then selected to be tested against Bacillus cereus (Gram+), Staphylococcus aureus (Gram+) and Salmonella typhimurium (Gram−) and their effect was compared using antibiotics as control. Screening results showed potential antibacterial activity of the tested plant extracts against the screened bacterial strains. It was found that methanol extracts exhibited higher antibacterial activity than aqueous extracts. Methanol extract of Jatropha showed the highest inhibition zone against Staphylococcus aureus (Gram+) with 24.00 mm diameter, compared to the other plant extracts followed by clove. Meanwhile, the inhibition zones of methanol extracts of Jojoba and Ginger were the same (12mm).The Gram-positive bacteria were found to be more sensitive to aqueous and methanol extracts than Gram-negative bacteria.

Keywords: Antibacterial activity, Food-borne pathogenic bacteria, Medicinal plants, Plant extracts.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1106769

References:

[1] A. Lucera, C. Costa, A. Conte, and M. A. Del Nobile, “Food applications of natural antimicrobial compounds,” Frontiers in Microbiology, vol. 3, Article 287, 1 – 13, 2012
[2] V. Areekul, P. Jiapiyasakul, and A. Chandrapatya, “In vitro Antimicrobial Screening of Selected Traditional Thai Plants,” Thai Journal of Agricultural Science, vol.42, no.2, 81-89, 2009.
[3] G. J. E. Nychas, C. C. Tassou, and P. Skandamis, “Antimicrobials from herbs and spices,” In S. M. Roller (Ed.), Natural antimicrobials for the minimal processing of foods, (pp. 176–200), New York: wood head Publishers, CRC Press, 2003.
[4] E. S. Al-Sheddi, “Pharmacognostical Study of Salvia merjamie Forsk growing in Saudi Arabia, PhD thesis. King Saud University, 2009.
[5] R. M. Mariita, C. K. P. O. Ogal, N. O. Oguge, and P. O. Okemo, “Methanol extract of three medicinal plants from Samburu in northern Kenya show significant Antimycobacterial, antibacterial and antifungal properties,” Research journal of medicinal plants, vol. 5, no. 1, 54-64, 2011.
[6] S. S. Thalwal, A. Gupta, Abhimanyu, N. Saini, S. K. Patel, and L. Kapoor, “Antimicrobial activity of traditionally used herbs against human pathogens,” International Journal of Biomedical Research, vol.4, no. 6, 236-240, 2013.
[7] B. Mahesh, and S. Satish, “Antimicrobial activity of some important medicinal plant against plant and human pathogens.” World Journal of Agricultural Science, vol.4, pp 839-843, 2008.
[8] T. Selvamohan, V. Ramadas, and S. S. S. Kishore, “Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria.” Advances in Applied Science Research, vol. 3, no.5, pp 3374-3381, 2012.
[9] M. Škrinjar, and N.T. Nemet, “Antimicrobial effects of spices and herbs essential oils,” Acta Periodica technologica, vol. 40, pp195-209, 2009. Jatropha,
[10] J. Billing and P.W. Sherman, “Antimicrobial Functions of Spices: why some like it hot.” The Quarterly Review of Biology Vol. 73, No. 1 (Mar., 1998): pp. 3-49. Jester. (Cited 2012 July 30). Available from: [11] M. Ha. Rasool, “Antimicrobial activity of plant essential oils against the growth of Escherichia coli,” Journal of Pharmacy, volume 3, Issue 6, pp 01-06, 2013.
[12] N. M. A. Chaudhry, and P. Tariq, “Antimicrobial Activity of Cinnamonum cassia against diverse microbial flora with its nutritional and medicinal impacts,” Pakistan Journal of Botany, vol.38, no.1, 2006, 169-17, 2006.
[13] S. Prabuseenivasan, M. Jayakumar, and S. Ignacimuthu, “In vitro antibacterial activity of some plant essential oils,” BMC Complementary and Alternative Medicine 2006, 6:39 doi, (2006), 1186/1472-6882-6-39, this article is available from: http://www.biomedcentral.com/1472- 6882/6/39.
[14] V. O. Oyetayo, “Microbial load and antimicrobial property of two Nigerian herbal remedies,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 5, no.1, 74 – 78, 2008
[15] G. F. Gislene, Nascimento, J. Locatelli, P. C. Freitas, and G. L. Silva, “Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria,” Brazilian Journal of Microbiology, vol. 31, pp 247- 256, 2000.
[16] I, Gull, M. Saeed , H. Shaukat, S.M. Aslam , Z.Q., and Athar A.M. “Inhibitory Effect of Allium Sativum and Zingiber officinale Extracts on Clinically Important Drug Resistant Bacteria, “Annals of Clinical Microbiology and Antimicrobials. 27 April 2012. Biomed Central. (Cited 2012, July, 30). Available from: http://www.annclinmicrob.com. ezproxy.drew.edu/content/pdf/1476-0711-11-8.pdf>.
[17] E. Menghani, C. K. Ojha, R. S. Negi, Y. Agarwal, and A. Pareek, “Screening of Indian Medicinal Plants and their potentials as Antimicrobial Agents, “Global Journal of Science Frontier Research, Volume 11, Issue 2, Version 1.0 March 2011.
[18] M. I. Hayam, A. A. Abou-Arab and F. M. Abu Salem, “Antioxidant and antimicrobial effects of some natural plant extracts added to lamb patties during storage,” grasas y aceites, vol.62, no.2, 139-148, 2011.
[19] M. A.S. Ferial, and H. M. Ibrahim, “Antimicrobial Activity and Phytochemicals Screening of Jojoba (Simmondsia chinensis) Root Extracts and Latex,” International journal of Biological, Veterinary , Agricultural and Food Engineering, vol. 8 , no.5, 507-512, 2014.
[20] S. M. Al-Reza, A. Rahman, J. Lee, and S.C. Kang,, “ Potential roles of essential oil and organic extracts of Zizy phus jujube in inhibiting foodborne pathogens,” Food Chemistry, vol. 119, pp 981–986, 2010.
[21] S. Hawash, N. Kamal, F. Zaher, O. Kenawi, and G. El Diwani, “Biodiesel fuel from Jatropha oil via non catalytic supercritical methanol transesterification,” Fuel, 88, 579-582, 2009.
[22] S. Rachana, A. Tarun, R. Rinki, A. Neha, and R. Meghna, “Comparative Analysis of Antibacterial Activity of Jatropha curcas Fruit,” Journal of Pharmaceutical and Biomedical Science, vol. 15, no. 12, 1- 4, 2012.
[23] Y. Li, C. Xu, Q. Zhang, J. Y. Liu, and R. X. Tan, “In vitro anti- Helicobacter pylori action of 30 Chinese herbal medicines used to treat ulcer diseases,” Journal of Ethno pharmacology, vol 98, 329-333, 2005.
[24] K. Chaieb, H. Hajlaoui, T. Zmantar, A. B. Kahla-Nakbi, M. Rouabhia, K. Mahdouani and A. Bakhrouf., “The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae)” A short review., Phytotheraby Research vol. 21, 501-506, 2007.
[25] Y. J. Fu, Y. G. Zu, L. Y. Chen, X. G. Shi , Z. Wang , S. Sun and T. Efferth “Antimicrobial activity of clove and rosemary essential oils alone and in combination,” Phytotheraby Research vol. 21, 989- 994, 2007
[26] M. O'Hara, D. Kiefer, K. Farrell, and K. Kemper, “A review of 12 commonly used medicinal herbs, “Arch Fam Med, vol. 7, 523 -536, 1998
[27] S. P. Malu, G.O. Obochi, E. N. Tawo, and B. E. Nyong, “Antibacterial activity and medicinal properties of ginger (Zingibre officinale),” Global Journal of Pure and Applied Sciences, vol. 15, 365-368, 2009
[28] A. D. Sebiomo, A. O. Awosanya, F.E. Awotona, and A. J. Ajayi, “Comparative studies of antibacterial effect of some antibiotics and ginger (Zingibre officinale) on two pathogenic bacteria, Journal of Microbiology and Antimicrobial, vol.3, 18- 22, 2011
[29] H.S. Lee, and Y.J. Ahn, “Growth-Inhibiting effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria,” Journal of Agricultural and Food Chemistry, vol.46, 8-12, 1998
[30] F. Sáez, and E. Stahl-Biskup, Essential oil polymorphism in the genus Thymus, In: Thyme: The Genus Thymus (Eds. E. Stahl-Biskup and F. Sáez), 124-143, 2002 Taylor and Francis, London and New York
[31] H. Baydar, O, Sagdic, O. Gulcan, and T. karadogan ,“Antibacterial activity and composition of essential oils from Origanum, Thymbra, an Satureja species with commercial importance in Turkey,” Food Control vol. 15,169-172,2004
[32] M. A. S. Ferial and M. I. Hayam, “Dry fermented buffalo sausage with sage oil extract: Safety and quality,” GRASAS Y ACEITES, vol.61, no.1, 76-87, 2010.
[33] A A. Khadri, M. Neffati, S. Smiti , P. Falé, A.R.L. Lino, M.L.M. Serralheiro, and M.E.M. Araùjo, “Antioxidant, antiacetylcholine sterase and antimicrobial activities of Cymbopogon schoenanthus L. Spreng (lemon grass) from Tunisia,” LWT– Journal of Food Science Technology, vol. 43, 331-336,2010.
[34] F. Sahin, F., M. Gulluce, D. Daferera, A. Sokmen, M. Sokmen, M. Polissiou, G. Agar, and H.Ozer, “Biological activities of the essential oils and methanol extract of Origanum vulgare spp. vulgar in the Eastern Anatolia region of Turkey,” Food Control , vol.15, 549-557, 2004
[35] S. Y. Daniyan, and H. B. Muhammad, "Evaluation of the antimicrobial activities and phytochemical properties of extracts of Tamaridus indica against some diseases causing bacteria" African Journal of Biotechnology, vol. 7, no.14, pp. 2451-2453, 18 July, 2008.
[36] V. K. Bajpai, A. Rahman, A., and S. C. Kang, “Chemical composition and inhibitory parameters of essential oil and extracts of Nandina domestica Thunb. to control food-borne pathogenic and spoilage bacteria,” International Journal of Food Microbiology, 125, 117–12, 2008.
[37] I. Bhattacharjee, S. K. Chatterjee, S. Chatterjee, and G. Chandra, “Antibacteria potentiality of Argemone mexicana solvent extracts against some pathogenic bacteria. Mem◌ rias do Instituto Oswaldo Cruz, vol.101, 645–648, 2006.
[38] B. Ji-Hyun, “Antimicrobial effect of Portulaca oleracea extracts on food-born pathogens,” Journal of food science and nutrition, vol. 9, 306– 311, 2004.
[39] A. Sher, “Antimicrobial activity of natural products from medicinal plants,” Gomal Journal of Medical Science, vol. 7, 72-78, 2009.
[40] P. C. B. Turnbull, and J.M. Kramer, Bacillus. In: Barlows, A., Hausler Jr., W. J., Herrmann, K.L., Isenberg, H.D., Shadomy, H.J. (Eds.), Manuals of Clinical Microbiology, 5th ed. American Society of Microbiology, Washington DC, pp. 345–355, 1991.
[41] P. Kumar, S. Chauhan, H. Padh, and M. Rajani, “Search for antibacterial and antifungal agents from selected Indian medicinal plants,” Journal of Ethnopharmacology, vol.107, 182-188, 2006.
[42] S. Calsamiglia, M. Busquet, P. W. Cardozo, L. Castillejos, and A. Ferret, “Invited Review: Essential oils as modifiers of rumen microbial fermentation,” Journal of Dairy Science, vol. 90, 2580–2595, 2007.