Potential of Safflower (Carthamus tinctorius L.) for Phytoremedation of Soils Contaminated with Heavy Metals

Authors: Violina R. Angelova, Vanja I. Akova, Stefan V. Krustev, Krasimir I. Ivanov

Abstract:

A field study was conducted to evaluate the efficacy of safflower plant for phytoremediation of contaminated soils. The experiment was performed on an agricultural fields contaminated by the Non-Ferrous-Metal Works near Plovdiv, Bulgaria. Field experiments with randomized complete block design with five treatments (control, compost amendments added at 20 and 40 t/daa, and vermicompost amendments added at 20 and 40 t/daa) were carried out. The quality of safflower seeds and oil (heavy metals and fatty acid composition) were determined. Tested organic amendments significantly influenced the chemical composition of safflower seeds and oil. The compost and vermicompost treatments significantly reduced heavy metals concentration in safflower seeds and oils, but the effect differed among them. Addition of vermicompost and compost leads to an increase in the content of palmitic acid and linoleic acid, and a decrease in the stearic and oleic acids compared with the control. A significant increase in the quantity of saturated acids was observed in the variants with 20 t/daa of compost and 20 t/daa of vermicompost (9.1 and 8.9% relative to the control). Safflower is a plant which is tolerant to heavy metals and can be successfully used in the phytoremediation of heavy metal contaminated soils. The processing of seeds to oil and using the obtained oil for nutritional purposes will greatly reduce the cost of phytoremediation.

Keywords: Heavy metals, organic amendments, phytoremediation, safflower.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1106637

References:

[1] A. P. G. C. Marques, R.S. Oliveira, A. O. S. S. Rangel, and P. M. L. Castro, “Application of manure and compost to contaminated soils and its effect on zinc accumulation by Solanum nigrum inoculated with arbuscular mycorrhizal fungi”, Environmental Pollution, vol.151, pp.608–620, 2008.
[2] N. Haque, J. R, Peralta-Videa, G. L. Jones, T. E. Gill, and J. L. Gardea- Torresdey, “Screening the phytoremediation potential of desert broom (Baccharis sarothroides Gray) growing on mine tailings in Arizona, USA”, Environmental Pollution, vol.153, pp.362–368, 2008.
[3] I. D. Pulford and C. Watson, “Phytoremediation of heavy metalcontaminated land by trees--a review”, Environment International, vol.29, pp.528–540, 2003.
[4] R. L. Chaney, M. Malik, Y. M. Li, S. L. Brown, E. P. Brewer, and J. S. Angle, “Phytoremediation of soils metals”, Current Opinion in Biotechnology, 8, pp.279–284, 1997.
[5] J. Ciura, M. Poniedzialek, A. Sekara and E. Jedrszezyk, “The Possibility of Using Crops as Metal Phytoremediants”, Polish Journal of Environmental Studies, vol.14, pp.17-20, 2005.
[6] M. I. Lone, H. Zhen-Li, P. J. Stoffella, and Y. Xiao, “Phytoremediation of heavy metal polluted soils and water: Progresses and perspectives”, Journal of Zhejiang University Sci., B. 9, pp. 210-220, 2008.
[7] X. E. Yang, H. Y. Peng, and L. Y. Jiang, “Phytoremediation of Copper from contaminated soil by Elsholtzia splendens as affected by EDTA, citric acid, and compost”, International Journal of Phytoremediation, vol.7, pp. 69-83, 2005.
[8] M. Komarek, P. Tlustos, J. Szakova, W. Richner, M. Brodbeck, and M. Sennhauser, “The use of maize and poplar in chelant-enhanced phytoextraction of lead from contaminated agricultural soils”, Chemosphere, vol.67, pp.640–651, 2007.
[9] A. Fässler, B. H. Robinson, S. K. Gupta, and R. Schulin, “Phytomanagement of metal-contaminated agricultural land using sunflower, maize and tobacco”, Nutrient Cycling in Agroecosystems, vol.87, pp. 339-352, 2010.
[10] S. Kirkova, Y. Dyulgerski, and T. Milanova, “Properties of new varieties and lines Burley tobacco investigation on consumables”, Bulgarian Journal of Agricultural Science, vol.20, pp. 643-646, 2014.
[11] H. Bozukov, S. Kirkova, and P. Zaprjanova, “Study of technologically unusable tobacco waste and practical solutions for its recovery”, Тутун/Tobaccо, vol.62, pp.109-114, 2014.
[12] Y. Dyulgerski, S. Kirkova, and T.Milanova, “Comparative analysis of Expert evaluation and Chemical composition of Varieties and lines Burley Tobacco”, Agricultural Science, vol.46, pp.47-53, 2013.
[13] S. Pandeliev, L. Angelov, B. Stalev, and M. Papanikolau, “Szanse ekologicznej uprawy winorosli w poludniowych winiarskich regionach bulgarii”, in 2010 Proc. IV Ogolnopolska Konferencja Winiarska. Nowosci w uprawie winorosli I produkcji win, Sulechow, pp. 99-109.
[14] V. Heuzé, G. Tran, P. Chapoutot, D. Bastianelli, F. Lebas, and D. Renaudeau, “Safflower (Carthamus tinctorius) seeds and oil meal”. Feedipedia.org. A programme by INRA, CIRAD, AFZ and FAO, 2012.
[15] A. B. Rahamatalla, E. E. Babiker, A. G. Krishna, and A. H. El Tinay, “Changes in chemical composition, minerals and amino acids during seed growth and development of four safflower cultivars”, Plant Foods for Human Nutrition, vol.52, pp. 161–170, 1988.
[16] A. B. Rahamatalla, E. E. Babiker, A. G. Krishna, and A. H. El Tinay, “Changes in fatty acids composition during seed growth and physicochemical characteristics of oil extracted from four safflower cultivars”, Plant Foods for Human Nutrition, vol.56, pp. 385–395, 2001.
[17] GRDC, “Raising the bar with better safflower agronomy”, GRDC - Grain research and development corporation, 2010, pp.39.
[18] V.Angelova, R. Ivanova, J. Todorov, K. Ivanov, M. Perifanova-Nemska, G. Uzunovа, and V. Ivanova, “The effect of organic amendments on uptake on heavy metals in safflower (Carthamus tinctorius L.)”, in Proceeding 6th Symposium on recycling technologies and sustainable development, September 18 - 21, Soko Banja, Srbija, pp.331-338, 2011.
[19] R. Clemente, D.J. Walker, and M.P. Bernal, “Uptake of heavy metals and As by Brassica juncea grown in a contamination soil in Arnalcollar (Spain): The effect of soil amendments”, Environmental Pollution, vol.136, pp.46 – 58, 2005.
[20] V.Angelova, M. Petkova, K. Ivanov, and P. Zaprjanova, “Effects of Organic Soil Amendments on Soil Physical and Chemical Properties”, in Proceeding 6th Symposium on recycling technologies and sustainable development, September 18 – 21, Soko Banja, Srbija, pp.315-322, 2011.
[21] D. J. Walker, R. Clemente, A. Roig, and M. P. Bernal, “The effect of soil amendments on heavy metal bioavailability in two contaminated Mediterranean soils”, Environmental Pollution, vol.22, pp.303 – 312, 2003.
[22] D. J. Walker, R. Clemente, and M. P. Bernal, “Contrasting effects of manure and compost on soil pH, heavy metal availability and growth of Chenopodium album L. in a soil contaminated by pyritic mine waste”. Chemosphere, vol.57, 215- 224, 2004.
[23] Animal feeding stuffs. Determination of nitrogen content and calculation of crude protein content. Kjeldahl method, BS EN ISO 5983-1,2005.
[24] Animal and vegetable fat and oils – Preparation of methyl esters of fatty acids. ISO 5509, 2000.
[25] Animal and vegetable fat and oils – Determination of methyl esters of fatty acids (Gas chromatographic method). ISO 5508, 2000.
[26] G. Barancikova, and J. Makovnikova, “The influence of humic acid quality on sorption and mobility of heavy metals”, Plant Soil Environ., vol.49, pp.565-571, 2003.
[27] M. M. Kononova, Soil Organic Matter. Pergamon Press, Oxford. 1966, 2nd edition, 1966.
[28] H. J. Hapke, “Metal accumulation in food chain and Load of feed and food”, in Metals and their compounds in the environment. Occurrence, analysis, and biological relevance, E. Merian, Ed. New York: Weinheim, 1991, pp. 469-479.
[29] R. L. Chaney,“Toxic element accumulation in soils and crops: protecting soil fertility and agricultural food-chains”, in: Inorganic contaminants in the Vadose Zone, B. Bar-Yosef, NJ Barrow, J. Goldshmid J, Eds. Berlin: Springer-Verlag, 1989, pp.140 –158.
[30] M. Penumetcha, N. Khan, and S. Parthasarathy, “Dietary oxidized fatty acids: An atherogenic rise”, J. Lipid Res., vol.41, pp. 1473-1480, 2000.
[31] L. Velasco, and J. M. Fernandez-Martinez, “Breeding for oil quality in safflower”, in 2001 Proceedings of the 5th International Safflower Conference. Williston, North Dakota and Sidney, Montana, USA, 133- 137.
[32] R. P. Mensink, E. H. M. Temme, and G. Hornstra, “Dietary saturated and trans fatty acids and lipoprotein metabolism”, Ann. Med., vol.26, pp.461—464, 1994.
[33] M. R. Sabzalian, G. Saeidi, and A. Mirloh, “Oil content and fatty acid composition in seeds of three safflower species”, J Am Oil Chem Soc., vol.85,717–721, 2008.
[34] E. Petcu, A. Arsintescu, and D. Stanciu, “The effect of drought stress on fatty acid composition in some Romanian sunflower hybrids”, Romanian Agricultural Research, vol.15, pp. 39-43, 2001.
[35] E.S. Bassil, and S.R. Kaffka, “Response of safflower (Carthamus tinctorius L.) to saline soils and irrigation: I.Consumptive water use”, Agric. Water Manag., vol.54, pp.67- 80, 2002.
[36] Z. Zhang, and Y. Chen, “Studies on adaptability of safflower germplasms in Xinjiang, China”, in VIth International Safflower Conference, Istanbul 6-10 June 2005. pp.132-139.
[37] S. D. Koutroubas, and D. K. Papadoska, “Adaptation, grain yield and oil content of safflower in Greece”, in VIth International Safflower Conference, Istanbul 6-10 June 2005, pp. 161-167.
[38] P. B. Gawand, S. I. Tambe, and B. N. Reddy, “Evaluation of productivity of safflower cultivars under moisture and nutrient management in rainfeed vertisols”, in VIth International Safflower Conference, Istanbul 6-10 June 2005, pp.205-209.
[39] B. Arslan, and M. Küçük, “Oil content and fatty acid composition of some safflower cultivars in Van (Turkey)”, in VIth International Safflower Conference, Istanbul 6-10 June 2005. pp. 167-175.
[40] V. Kumar, “Nitrogen economy in Indian mustard through use of Azotobacter chroococcum”, Crop Research, vol.8, pp. 449-452, 1994.
[41] S. C. Wu, Z. H. Cao, Z. C. Li, and K. C. Cheung, “Effect of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial”, Geoderma, vol.125, pp.155-166, 2005.
[42] J. D. Scheiner, F.H. Gutiérrez-Boem, and R.S. Lavado, “Sunflower nitrogen requirement and 15N fertilizer recovery in Western Pampas, Argentina”, Eur. J. Agron., vol.17, pp.73-79, 2002.
[43] D. T. Canvin, The effect of temperature on the oil contenit and fatty acid comilpositioln of theoils from several oil seed crops. Canadian. J.Botan., vol.43, pp. 63-69, 1965.
[44] G. Nagaraj, “Safflower seed composition and oil quality - A review”, in Proc. 3rd International Safflower Conference, 14-18 June 1993, Beijing, China.
[45] M. A. Munir, M.A. Malik and M.F. Saleem, “Impact of integration of crop manuring and nitrogen application on growth, yield and quality of spring planted sunflower (Helianthus annuus L.)”, Pak. J. Bot., vol.39, pp. 441-449, 2007.
[46] E. Petcu, E., A. Arsintescu, and D. Stanciu, “The effect of hydric stress on some characteristics of sunflower plants”, Romanian Agricultural Research, vol.16, pp. 15-22, 2001.
[47] P.Akbari, A. Ghalavand, A. M. Modarres Sanavy, and M. Agha Alikhani, “The effect of biofertilizers, nitrogen fertilizer and farmyard manure on grain yield and seed quality of sunflower (Helianthus annus L.)”, Journal of Agricultural Technology, vol.7(1), pp.173-184, 2011.
[48] G. I. Seiler, “Wild annual Helianthus anomalus and H. deserticola for improving oilcontent and quality in sunflower”, Industrial Crops and Products, vol.25, pp.95–100, 2007.
[49] G. I. Seiler, “Analysis of the relationships of environmental factors with seed oil and fatty acid concentrations of wild annual sunflower”, Field Crops Research, vol.15, 57–72, 1986.
[50] U. Gecgel, M. Demirci, E. Esendal, and M. Tasan, “Fatty acid composition of the oil from developing seeds of different varieties of safflower (Carthamus tinctorius L.)”, J Am Oil Chem Soc., vol.84, pp.47–54, 2007.
[51] Z. Zongwen, and C. Yuehua, “Studies on the ecological adaptability of safflower germplasms”, in Proceedings Vlth International Safflowers Conference, 6-10 June 1997, Istanbul, Turkey.
[52] R. K. Downey, and G. F. W. Rakow, “Rapeseed and mustard”, in Principles of Cultivar Development, W. R. Fehr, Ed. New York Macmillan Publishing Co., 1987, pp. 437-886.
[53] N. F. Kheir, E. Z. Harb, H. A. Moursi, and S. H. El-Gayar, “Effect of Salinity and Fertilization on Flax Plants (Linum usitatissimum L.). II. Chemical Composition”, Bull.Faculty Agriculture (Univ. Cairo), vol. 42, pp. 57–70, 1991.
[54] B. T. Steer, and G. I. Seiler, “Changes in fatty acid composition of sunflower (Helianthus annuus L.) seeds in response to time of nitrogen application, supply rates and defoliation”, Journal of the Science of Food and Agriculture, vol.51, pp. 11-26, 1990.