LIFirr with an Indicator of Microbial Activity in Paraffinic Oil

Authors: M. P. Casiraghi, C. M. Quintella, P. Almeida

Abstract:

Paraffinic oils were submitted to microbial action. The microorganisms consisted of bacteria of the genera Pseudomonas sp. and Bacillus lincheniforms. The alterations in interfacial tension were determined using a tensometer and applying the hanging drop technique at room temperature (299 K ±275 K). The alteration in the constitution of the paraffins was evaluated by means of gas chromatography. The microbial activity was observed to reduce interfacial tension by 54 to 78%, as well as consuming the paraffins C19 to C29 and producing paraffins C36 to C44. The LIFirr technique made it possible to determine the microbial action quickly.

Keywords: Paraffins, biosurfactants, LIFirr.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1100356

References:

[1] P. F. Almeida, R. C. Moreira, A. K. Guimarães, A. S. Carvalho, C. M. Quintella, M. C. A. Esperidião, C. A, Taft, “Selection and application of microorganisms to implovise oil recovery”, Eng. Life Sci., vol. 4, no. 4, pp. 319-324, 2004.
[2] G. Andreatta, C. C. Gonçalves, G. Buffin, N. Bostrom, C. M. Quintella, F. Arteagalarios, A. Pérez, O. C. Mullins, “Nanoaggregates and structure-function relations in asphaltenes,” Energ. Fuel., v. 19, no. 4, pp. 1282-1289, 2005.
[3] R. M. Atlas et al., “Response of microbial-populations to environmental disturbance”, Microbiology Reviews, vol. 45, pp. 249-256, 1981.
[4] F. S. Brito, M. S. Teixeira, A. P. Musse, C. M. Quintella, Anais da 25ª Reunião da Sociedade Brasileira de Química, Brazil, 2002.
[5] E. Buenrostro-Gonzalez, H. Groenzin, C. Lira-Galeana, and O. C. Mullins, “The overriding chemical principles that define asphaltenes,” Energ. Fuel.,v.15, pp. 972-978, Jul. 2001.
[6] A. Cosulchi, E. Garciafigueroa, A. García-Bórquez, E. Reguera, Yee-H. Madeira, V. H. Lara, P. Bosch, “Petroleum solid adherence on tubing surface,” Fuel, v. 80, pp. 1963-1968, Feb. 2001.
[7] D. L. Dorset, J. Hanlon, G. Karet, “Epitaxy and structure of paraffindiluent eutectics,” J. Phys. Chem. B, v. 22, no. 5, pp2169-2176, May. 1989.
[8] G. D. Floodgate, “The preservation of marine bacteria,” Aquatic Microbial Ecology, vol. 24, no. 1, pp. 87-93, Nov. 1961.
[9] D. S. Francy, R. L. Raymund, C. H. Ward, “Emulsification of hydrocarbons by subsurface bacteria,” Journal of Industrial Microbiology, v. 8, pp. 237-246, 1991.
[10] Y. H. Jang, M. Blanco, J. Creek, Y. C. Tang, W. A. J. Goddard, “Wax inhibition by comb-like polymers: Support of the incorporationperturbation mechanism from molecular dynamics simulations,” Phys. Chem. B, v. 111, no. 46, pp. 12173-13179, 2007.
[11] J. G. Leahy, R. R. Cowell, “Microbial-degradation of hydrocarbons in the environment,” Microbiology Review, vol. 54, pp. 305-355, 1990.
[12] C.M Quintella, A. P. S. Musse, M. T. P. O. Castro, J. C. Scaiano, L. Mikelsons, Y. N. Watanabe, ”Polymeric surfaces for heavy oil pipelines to inhibit wax deposition: PP, EVA28, and HDPE,” Energ. Fuel., v. 20, pp. 620-624, 2006.
[13] E. B. Silva, C. M. Quintella, A. P. Musse, H. Fraga, L. Friedriech, “Determinação das regiões de oxidação do PEBD através da alteração da tensão interfacial dinâmica por PLF-FI,” Anais da 25ª Reunião da Sociedade Brasileira de Química Brazil, 2005.
[14] C. M. Quintella, L. A. Friedrich, A. P. S. Musse, A. M. V. Lima, M. A. Macedo, R. M. Silva, I. M. Pepe, E. B. Silva, L. C. S. Soares, “Wettability under imposed flow as a function of the baking temperatures of a DGEBA epoxy resin used in the crude oil industry,” Energ. Fuel., v. 21, no. 4, pp. 2311-2316, 2007.
[15] C. M. Quintella, A. M. V. Lima, E. B. Silva, “Selective inhibition of paraffin deposition under high flow rate as a function of the crude oil paraffin type and content by fluorescence depolarization: PP and HDPE,” J. Phys. Chem. v. 110, no. 14, pp. 7587-7591, 2006.
[16] C. M. Quintella, C. C. Gonçalves, I. Pepe, A. M. V. Lima, A. P. Musse, “Intermolecular alignment dependence of ethylene glycol flow on the chemical nature of the solid surface (Borosilicate and SnO2),” J. Braz. Chem. Soc., v. 12, no. 6, 2001.
[17] C. M. Quintella, C. C. Gonçalves, I. Pepe, A. M. V. Lima, A. P. Musse, “Automated system to acquire fluorescence, polarization and anisotropy maps within liquid flows,”Journal of Automated Methodos & Management in Chemistry, v. 24, no. 2, pp. 31-39, Jan. 2002.
[18] A. J. Rosa, R. Carvalho, J. A. D. Xavier, “Engenharia de Reservatórios de Petróleo”, 2006.
[19] V. G. Grishchenkov, R. T. Townsed, T. J. McDonald, R. L. Autenrieth, J. S. Bonner, A. M. Boronin, “Degradation of petroleum hydrocarbons by facultative anaerobic bacteria under aerobic and anaerobic conditions,” Process Biochem., v. 35, pp. 889-896, Nov. 2000.
[20] G. F. Cruz, A. J. Marsaioli, “Processos naturais de biodegradação do petróleo em reservatórios”, Quim. Nova, vol. 35, no. 8, pp. 1628-1634, 2012.