In vivo Iron Availability and Profile Lipid Composition in Anemic Rats Fed on Diets with Black Rice Bran Extract
Authors: E. P. Nurlaili, M. Astuti, Y. Marsono, S. Naruki
Iron is an essential nutrient with limited bioavailability. Nutritional anemia caused mainly by iron deficiency is the most recognized nutritional problem in both countries as well as affluent societies. Rice (Oryza sativa L.) has become the most important cereal crop for the improvement of human health due to the starch, protein, oil, and the majority of micronutrients, particularly in Asian countries. In this study, the iron availability and profile lipid were evaluated for the extracts from Cibeusi varieties (black rices) of ancient rice brans. Results: The quality of K, B, R, E diets groups shows the same effect on the growth of rats. Hematocrit and MCHC levels of rats fed K, B, R and E diets were not significantly (P<0.05). MCV and MCH levels of rats K, B, R were significantly (P<0.05) with E groups but rats K, B, R were not significantly (P<0.05). The iron content in the serum of rats fed with K, B, R and E diets were not significantly (P<0.05). The highest level of iron in the serum was founded in the B group. The iron content in the liver of rats fed with K, B, R and E diets were not significantly (P<0.05). The highest level of iron in the liver was founded in the R group. HDL cholesterol levels were significantly (P<0.05) between rats of fed B, E with K, R, but K and R were not significantly (P<0.05). LDL cholesterol levels of rats fed K and E significantly (P<0.05) with B and R. Conclusions: the bran of pigmented rice varieties has, with some exceptions, greater antioxidant and free-radical scavenging activities. The results also show that pigmented rice extracts acted as prooxidants in the lipid peroxidation assay, possibly by mechanisms described for the pro-oxidant activities of tocopherol and ascorbic. Pigmented rice bran extracts more effectively increases iron stores and reduces the prevalence of iron deficiency.
Keywords: Anemia, black rice bran extract, iron, profile lipid.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1100012Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1842
 Anonym, (2002). Black Rice Concentrate, A Natural Source of Bioavailable Iron and Antioxidants. [email protected].; www.dracoherbs.com.
 Liu, R. H. (2007). Whole grain phytochemicals and health. Journal of Cereal Science, 46, 207–219.
 Butsat, S., and Siriamornpun, S. (2010). Antioxidant capacities and phenolic compounds of the husk, bran and endosperm of Thai rice. Food Chemistry, 119, 606–613.
 Aguilar-Garcia, C., Gavino, G., Baragano-Mosqueda, M., Hevia, P., and Gavino, V. (2007). Correlation of tocopherol, tocotrienol, c-oryzanol and total polyphenol content in rice bran with different antioxidant capacity assays. Food Chemistry, 102, 1228–1232.
 Adom, K. K., and Liu, R. H. (2002). Antioxidant activity of grains. Journal of Agricultural and Food Chemistry, 50, 6182–6187.
 Yawadio, R., Tanimori, S., and Morita, N. (2007). Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food Chemistry, 101, 1616–1625
 Miller, A., and Engel, K.H. (2006). Content of c-oryzanol and composition of sterylferulates in brown rice (Oryza sativa L.) of European origin. Journal of Agricultural and Food Chemistry, 54, 8127– 8133.
 Nam, S. H., Choi, S. P., Kang, M. Y., Kozukue, N., & Friedman, M. (2005). Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays. Journal of Agricultural and Food Chemistry, 53, 816–822
 Ling WH, Wang L.L, Ma J. (2002). Supplementation of the black rice outer layer fraction to rabbits decreases atherosclerotic plaque formation and increases antioxidant status. J Nutr 132: 20–26.
 Xia M., Ling WH., Ma J, Kitts D.D., Zawistowski J. (2003). Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein E deficient mice. J Nutr 133: 744–751
 Stohs SJ, Bagghi D. (1995). Oxidative mechanisms in the toxicity of metal ions. Free Rad BiolMed.; 18:321-36.
 Agil A, Fuller CJ, Jialal I. (1995). Susceptibility of plasma to ferrous iron/hydrogen peroxide- mediated oxidation: demonstration of a possible Fenton reaction. Clin. Chem; 41:220-5.
 Balla G, Jacob HS, Eaton JW, Belcher JD, Vercellotti GM. (1991). Haemin: a possible physiological mediator of low density lipoprotein oxidation and endothelial injury. Arterioscler Thromb; 11:1700-11.
 Herhsko C. (1989). Mechanism of iron toxicity and its possible role in red cell membrane damage. SeminHematol; 26:277-85.
 Jacobs P, Wormald L. (1979). The bioavailability of an iron polymaltose complex for treatment of iron deficiency. Journal of Medicine; 10(4):279-285.
 Buettner, G. R. (1986). Ascorbate autoxidation in the presence of iron ad cnopper chelates. Free Radical Research Communications, 1(6), 349– 353.
 Yamauchi, R., Miyake, N., Kato, K., & Ueno, Y. (1993). Reaction of atocopherol with alkyl and alkylperoxyl radicals of methyl inoleate. Lipids, 28(3), 201–206.