Development of a New Polymeric Material with Controlled Surface Micro-Morphology Aimed for Biosensors Applications

Authors: Elham Farahmand, Fatimah Ibrahim, Samira Hosseini, Ivan Djordjevic, Leo. H. Koole

Abstract:

Compositions of different molar ratios of polymethylmethacrylate-co-methacrylic acid (PMMA-co-MAA) were synthesized via free-radical polymerization. Polymer coated surfaces have been produced on silicon wafers. Coated samples were analyzed by atomic force microscopy (AFM). The results have shown that the roughness of the surfaces have increased by increasing the molar ratio of monomer methacrylic acid (MAA). This study reveals that the gradual increase in surface roughness is due to the fact that carboxylic functional groups have been generated by MAA segments. Such surfaces can be desirable platforms for fabrication of the biosensors for detection of the viruses and diseases.

Keywords: Polymethylmethacrylate-co-methacrylic acid (PMMA-co-MAA), Polymeric material, Atomic Force Microscopy, roughness, carboxylic functional groups.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1099102

References:

[1] S. Hosseini, F. Ibrahim, I. Djordjevic, H. Rothan, R. Yusof, C. Marel and LH. Koole, “Synthesize and processing of ELISA polymer substitute: The influence of surface chemistry and morphology on detection sensitivity”, Applied Surface Science, 317, pp.630-638, 2014.
[2] Y. Jung, JY. Jeong, BH. Chung, “Recent advances in immobilization methods of antibodies on solid support”, Analyst, pp. 697-701. 2008.
[3] S. Hosseini, F. Ibrahim, I. Djordjevic, LH. Koole, “Polymethyl methacrylate-co-methacrylic acid coatings with controllable concentration of surface carboxyl groups: A novel approach in fabrication of polymeric platforms for potential bio-diagnostic devices”, Applied Surface Science, vol. 300, pp.43-50, 2014.
[4] S. Hosseini, F. Ibrahim, I. Djordjevic, LH. Koole, “Recent advances in surface functionalization techniques on polymethacrylate materials for optical biosensor applications”, Analyst, vol. 139, pp.2933-2943, 2014.
[5] E. Salonen and A. Vaher, Journal of immunological methods, Vol.30. pp. 209-218, 1979.
[6] F. Fixe, M. Dufva, P. Telleman, C.B.V. Christensen, “Functionalization of poly (methylmethacrylate)(PMMA) as a substrate for DNA microarray”, Nucleic Acids Res, vol. 32, 2004.
[7] K. Saralidze, C.S.J. van Hooy-Corstjens, L.H. Koole, M.L.W. Knetsch, “New acrylic microspheres for arterial embolization: combining radiopacity for precise localization with immobilized thrombin to trigger local blood coagulation”, Biomaterials, vol. 28, pp.2457–2464, 2007.
[8] Y. Jung, J.Y. Jeong, B.H. Chung, “Recent advances in immobilization methods of antibodies on solid supports”, Analyst, vol. 133, pp. 697– 701, 2008.
[9] Q. Lu, E. Danner, J.H. Waite, J.N. Israelachvili, H. Zeng, D.S. Hwang, “Adhesion of musselfoot proteins to different substrate surfaces”, Journal of The Royal Society Interface, vol.10, 2013.
[10] ] B.R. Coad, M. Jasieniak, S.S. Griesser, H.J. Griesser, “Controlled covalent surface immobilization of proteins and peptides using plasma methods”, Surface and Coatings Technology, 2013.
[11] A. Vesel, M. Mozetic, “Surface modification and ageing of PMMA polymer by oxygen plasma treatment”, Vacuum, Vol.86, pp. 634–637
[12] S.R. Wasserman, Y.T. Tao, G.M. Whitesides, “Structure and reactivity of alkysiloxane monolayers formed by reaction of alkyltrichlorosilanes on silicon substrates”, Langmuir, vol. 5, pp. 1074–1087, 1989.
[13] J.M. Goddard, J.H. Hotchkiss, “Polymer surface modification for the attachmentof bioactive compounds”, Progress in Polymer Science, 32, pp. 698–725, 2007.
[14] Y. Liu, W. Hu, Z. Lu, C.M. Li, “Photografted poly(methyl methacrylate)-basedhigh performance protein microarray for hepatitis B virus biomarker detectionin human serum”, MedChemComm, vol. 1, pp.132–135, 2010.
[15] F. Fixe, M. Dufva, P. Telleman, C.B.V. Christensen, “Functionalization ofpoly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays”, NucleicAcidsRes,vol. 32, 2004.
[16] K. Saralidze, C.S.J. van Hooy-Corstjens, L.H. Koole, M.L.W. Knetsch, “New acrylicmicrospheres for arterial embolization: combining radiopacity for preciselocalization with immobilized thrombin to trigger local blood coagulation”,Biomaterials, vol. 28, pp. 2457–2464, 2007.
[17] Y. Jung, J.Y. Jeong, B.H. Chung, “Recent advances in immobilization methods ofantibodies on solid supports”, Analyst, vol. 133, pp. 697–701, 2008.
[18] Y. Bai, C.G. Koh, M. Boreman, Y.-J. Juang, I.C. Tang, L.J. Lee, et al., “Surfacemodification for enhancing antibody binding on polymer-based microfluidicdevice for enzyme-linked immunosorbent assay”,Langmuirvol. 22, pp. 9458–9467, 2006.
[19] D. Sok, L.-J. Clarizia, L. Farris, M. McDonald, “Novel fluoroimmunoassay for ovarian cancer biomarker CA-125”, Anal. Bioanal. Chem. vol. 393, pp. 1521–1523, 2009.
[20] Q. Lu, E. Danner, J.H. Waite, J.N. Israelachvili, H. Zeng, D.S. Hwang, “Adhesion ofmussel foot proteins to different substrate surfaces”, Journal of The Royal SocietyInterface, vol. 10, 2013.
[21] B.R. Coad, M. Jasieniak, S.S. Griesser, H.J. Griesser, “Controlled covalent surfaceimmobilization of proteins and peptides using plasma methods, Surface andCoatings Technology” 2013.
[22] H. Wensink, F. Benito-Lopez, D.C. Hermes, W. Verboom, H.J.G.E. Gardeniers, D.N.Reinhoudt, et al., “Measuring reaction kinetics in a labon- a-chip by microcoilNMR”, Lab on a Chip, vol. 5, pp. 280–284, 2005.
[23] PE. Scopelliti , A. Borgonovo, M. Indrieri, L.Giorgetti, G. Bongiorno, et al, “The Effect of Surface Nanometre-Scale Morphology on Protein Adsorption”.Plos One, vol.5, Issue.7, 2010.