Isolation and Identification of Diacylglycerol Acyltransferase Type- 2 (GAT2) Genes from Three Egyptian Olive Cultivars
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 32769
Isolation and Identification of Diacylglycerol Acyltransferase Type- 2 (GAT2) Genes from Three Egyptian Olive Cultivars

Authors: Yahia I. Mohamed, Ahmed I. Marzouk, Mohamed A. Yacout

Abstract:

Aim of this work was to study the genetic basis for oil accumulation in olive fruit via tracking DGAT2 (Diacylglycerol acyltransferase type-2) gene in three Egyptian Origen Olive cultivars namely Toffahi, Hamed and Maraki using molecular marker techniques and bioinformatics tools. Results illustrate that, firstly: specific genomic band of Maraki cultivars was identified as DGAT2 (Diacylglycerol acyltransferase type-2) and identical for this gene in Olea europaea with 100% of similarity. Secondly, differential genomic band of Maraki cultivars which produced from RAPD fingerprinting technique reflected predicted distinguished sequence which identified as DGAT2 (Diacylglycerol acyltransferase type-2) in Fragaria vesca subsp. Vesca with 76% of sequential similarity. Third and finally, specific genomic specific band of Hamed cultivars was identified as two fragments, 1- Olea europaea cultivar Koroneiki diacylglycerol acyltransferase type 2 mRNA, complete cds with two matches regions with 99% or 2- Predicted: Fragaria vesca subsp. vesca diacylglycerol O-acyltransferase 2-like (LOC101313050), mRNA with 86 % of similarity.

Keywords: Olea europaea, fingerprinting, Diacylglycerol acyltransferase type- 2 (DGAT2).

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1326826

Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2364

References:


[1] S. Kumar; T. Kahlon and S. Chaudhary. A rapid screening for adulterants in olive oil using DNA barcodes, Food Chemi. 2011, 127,1335–1341
[2] C. Consolandi, L. Palmieri, M. Severgnini, E. Maestri, N. Marmiroli, C. Agrimonti, L. Baldoni, P. Donini, G. Bellis and B. Castiglioni. A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR-universal array analysis. Eur. Food Res. Technol. 2008, 227:1429–1438
[3] S. Pafundo, C. Agrimonti and N. Marmiroli. Traceability of plant contribution in olive oil by amplified fragment length polymorphisms. J. Agric. Food Chem. 2005, 53, 6995-7002.S.
[4] Pafundo, M. Busconi, C. Agrimonti, C. Fogher and M. Marmiroli.. Storage-time effects on olive oil DNA assessed by amplified fragments length polymorphisms. Food Chem. 2010, 123:787–793
[5] E. P. Kennedy. Biosynthesis of complex lipids. Federation Proceedings. 1961, 20, 934–940.
[6] J. Browse, and C. Somerville. Glycerolipid synthesis: biochemistry and regulation. Annu. Rev. of Plant Phys. and Plant Molec. Biol. 1991, 42, 467–506.
[7] C. Jako, A. Kumar, Y. D. Wei, J. T. Zou, D. L. Barton, E. M. Giblin, P. S. Covello and D. C, Taylor. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerolacyltransferase enhances seed oil content and seed weight. Plant Physi. 2001, 126, 861–874.
[8] S. C. Lung, and R. J. Weselake. Diacylglycerolacyltransferase: a key mediator of plant triacylglycerol synthesis. Lipids. 2006, 41, 1073–1088.
[9] B. G. Banials, K. Michael, I. Makariti, A. Kourti and P. Hatzopoulos. The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1. J. of Experi. Bot., 2011, 62 (2). 521–532.
[10] P. Hatzopoulos, G. Banilas, K. Giannoulia, F. Gazis, N. Nikoloudakis, D. Milioni and K Haralampidis. Breeding, molecular markers and molecular biology of the olive tree. Euro. J. of Lipid Scie. & Techno. 2002, 104, 574–586.
[11] S. Doveri and L. Baldoni. Olive. In: Kole C, ed. Genome mapping and molecular breeding in plants, fruits and nuts, Vol. IV. Berlin, Heidelberg: Springer-Verlag. 2007, 253–264.
[12] G. Banilas, and P. Hatzopoulos. Developmental perplexity of oil biosynthesis gene expression in olive. In: Berti L, Maury J, eds. Advances in olive resources. Kerala, India: Transworld Research Network, 2009, 1–22.
[13] G. Vergari, M. Patumi and G. Fontanazza. Use of RAPD markers in the characterization of olive germplasm. Olivae, 1996, 60:19-22.
[14] D.S. Hassawi and T. Hdeib. Genetic analysis of olive genotypes (Olea europaea L.) using random amplified polymorphic DNA (RAPD). J. Genet. & Breed. 2004, 58: 141-148.
[15] Y. I. Mohamed and M. Yacout. Random Amplified Polymorphic DNA (RAPD) Analysis of Olive (Olea europaea L.) cultivars grown in North west coast region of Egypt. Inter. J. of Envir. Engin. 2014,1 (2) 36-40.
[16] K. Giannoulia, K. Haralampids, Z. Poghosyan, D.J. Murphy and P Hatzopoulos. Differential expression of diacylglycerol acyltransferase (DGAT) genes in olive tissues. Biochemi. Soci. Transa. 2000, 28, 695– 697.
[17] K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker and D. J. Hawkins. DGAT2 is a new diacylglycerolacyltransferase gene family: purification, cloning, and expression in insect cells of two polypeptides from Mortierellaramanniana with diacylglycerolacyltransferase activity. J. of Biolo. Chemi. 2001, 276, 38862–38869.
[18] S. Saha, B. Enugutti, S. Rajakumari and R. Rajasekharan. Cytosolic triacylglycerol biosynthetic pathway in oilseeds: molecular cloning and expression of peanut cytosolic diacylglyerolacyltransferase. Plant Phys. 2006, 141, 1533–1543.
[19] M. Nelson. Lopera-Barrero, A. Jayme, P. Povh, R. Ribeiro, C. P. Gomes, B. Carolina. Jacometo and L. Tais da Silva. Comparison of DNA extraction protocols of fish fin and larvae samples: modified salt (NaCl) extraction. Cien. Inv. Agr. 2008, 35(1): 77-86.
[20] http://www.totallab.co
[21] http://www.ncbi.nlm.nin.gov/BLAST/
[22] http://www.Past. com
[23] www.chirimoyo. ac.uma.es/olea gen/