Search results for: R. Eddie Wilson
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 38

Search results for: R. Eddie Wilson

38 Quantifying Freeway Capacity Reductions by Rainfall Intensities Based on Stochastic Nature of Flow Breakdown

Authors: Hoyoung Lee, Dong-Kyu Kim, Seung-Young Kho, R. Eddie Wilson

Abstract:

This study quantifies a decrement in freeway capacity during rainfall. Traffic and rainfall data were gathered from Highway Agencies and Wunderground weather service. Three inter-urban freeway sections and its nearest weather stations were selected as experimental sites. Capacity analysis found reductions of maximum and mean pre-breakdown flow rates due to rainfall. The Kruskal-Wallis test also provided some evidence to suggest that the variance in the pre-breakdown flow rate is statistically insignificant. Potential application of this study lies in the operation of real time traffic management schemes such as Variable Speed Limits (VSL), Hard Shoulder Running (HSR), and Ramp Metering System (RMS), where speed or flow limits could be set based on a number of factors, including rainfall events and their intensities.

Keywords: Capacity randomness, flow breakdown, freeway capacity, rainfall.

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37 New Multi-Solid Thermodynamic Model for the Prediction of Wax Formation

Authors: Ehsan Ghanaei, Feridun Esmaeilzadeh, Jamshid Fathi Kaljahi

Abstract:

In the previous multi-solid models,¤ò approach is used for the calculation of fugacity in the liquid phase. For the first time, in the proposed multi-solid thermodynamic model,γ approach has been used for calculation of fugacity in the liquid mixture. Therefore, some activity coefficient models have been studied that the results show that the predictive Wilson model is more appropriate than others. The results demonstrate γ approach using the predictive Wilson model is in more agreement with experimental data than the previous multi-solid models. Also, by this method, generates a new approach for presenting stability analysis in phase equilibrium calculations. Meanwhile, the run time in γ approach is less than the previous methods used ¤ò approach. The results of the new model present 0.75 AAD % (Average Absolute Deviation) from the experimental data which is less than the results error of the previous multi-solid models obviously.

Keywords: Multi-solid thermodynamic model, PredictiveWilson model, Wax formation.

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36 Mutation Analysis of the ATP7B Gene in 43 Vietnamese Wilson’s Disease Patients

Authors: Huong M. T. Nguyen, Hoa A. P. Nguyen, Mai P. T. Nguyen, Ngoc D. Ngo, Van T. Ta, Hai T. Le, Chi V. Phan

Abstract:

Wilson’s disease (WD) is an autosomal recessive disorder of the copper metabolism, which is caused by a mutation in the copper-transporting P-type ATPase (ATP7B). The mechanism of this disease is the failure of hepatic excretion of copper to bile, and leads to copper deposits in the liver and other organs. The ATP7B gene is located on the long arm of chromosome 13 (13q14.3). This study aimed to investigate the gene mutation in the Vietnamese patients with WD, and make a presymptomatic diagnosis for their familial members. Forty-three WD patients and their 65 siblings were identified as having ATP7B gene mutations. Genomic DNA was extracted from peripheral blood samples; 21 exons and exon-intron boundaries of the ATP7B gene were analyzed by direct sequencing. We recognized four mutations ([R723=; H724Tfs*34], V1042Cfs*79, D1027H, and IVS6+3A>G) in the sum of 20 detectable mutations, accounting for 87.2% of the total. Mutation S105* was determined to have a high rate (32.6%) in this study. The hotspot regions of ATP7B were found at exons 2, 16, and 8, and intron 14, in 39.6 %, 11.6 %, 9.3%, and 7 % of patients, respectively. Among nine homozygote/compound heterozygote siblings of the patients with WD, three individuals were determined as asymptomatic by screening mutations of the probands. They would begin treatment after diagnosis. In conclusion, 20 different mutations were detected in 43 WD patients. Of this number, four novel mutations were explored, including [R723=; H724Tfs*34], V1042Cfs*79, D1027H, and IVS6+3A>G. The mutation S105* is the most prevalent and has been considered as a biomarker that can be used in a rapid detection assay for diagnosis of WD patients. Exons 2, 8, and 16, and intron 14 should be screened initially for WD patients in Vietnam. Based on risk profile for WD, genetic testing for presymptomatic patients is also useful in diagnosis and treatment.

Keywords: ATP7B gene, mutation detection, presymptomatic diagnosis, Vietnamese Wilson’s disease.

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35 Simulation of Activity Stream inside Energy Social Business Environment using Assemblage Theory and Simplicial Complex Tool

Authors: Eddie Soulier, Philippe Calvez, Florie Bugeaud, Francis Rousseaux, Jacky Legrand

Abstract:

Social, mobility and information aggregation inside business environment need to converge to reach the next step of collaboration to enhance interaction and innovation. The following article is based on the “Assemblage" concept seen as a framework to formalize new user interfaces and applications. The area of research is the Energy Social Business Environment, especially the Energy Smart Grids, which are considered as functional and technical foundations of the revolution of the Energy Sector of tomorrow. The assemblages are modelized by means of mereology and simplicial complexes. Its objective is to offer new central attention and decision-making tools to end-users.

Keywords: Activity Streams, Assemblage, Energy Social Business Environment, Simplicial Complex, Smart Grid

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34 Engineered Cement Composite Materials Characterization for Tunneling Applications

Authors: S. Boughanem, D. A. Jesson, M. J. Mulheron, P.A. Smith C. Eddie, S. Psomas, M. Rimes

Abstract:

Cements, which are intrinsically brittle materials, can exhibit a degree of pseudo-ductility when reinforced with a sufficient volume fraction of a fibrous phase. This class of materials, called Engineered Cement Composites (ECC) has the potential to be used in future tunneling applications where a level of pseudo-ductility is required to avoid brittle failures. However uncertainties remain regarding mechanical performance. Previous work has focused on comparatively thin specimens; however for future civil engineering applications, it is imperative that the behavior in tension of thicker specimens is understood. In the present work, specimens containing cement powder and admixtures have been manufactured following two different processes and tested in tension. Multiple matrix cracking has been observed during tensile testing, leading to a “strain-hardening" behavior, confirming the possible suitability of ECC material when used as thick sections (greater than 50mm) in tunneling applications.

Keywords: Cement composite, polymeric fibers, pseudoductility, test-geometry.

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33 A Conceptual Query-Driven Design Framework for Data Warehouse

Authors: Resmi Nair, Campbell Wilson, Bala Srinivasan

Abstract:

Data warehouse is a dedicated database used for querying and reporting. Queries in this environment show special characteristics such as multidimensionality and aggregation. Exploiting the nature of queries, in this paper we propose a query driven design framework. The proposed framework is general and allows a designer to generate a schema based on a set of queries.

Keywords: Conceptual schema, data warehouse, queries, requirements.

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32 ANDASA: A Web Environment for Artistic and Cultural Data Representation

Authors: Carole Salis, Marie F. Wilson, Fabrizio Murgia, Cristian Lai, Franco Atzori, Giulia M. Orrù

Abstract:

ANDASA is a knowledge management platform for the capitalization of knowledge and cultural assets for the artistic and cultural sectors. It was built based on the priorities expressed by the participating artists. Through mapping artistic activities and specificities, it enables to highlight various aspects of the artistic research and production. Such instrument will contribute to create networks and partnerships, as it enables to evidentiate who does what, in what field, using which methodology. The platform is accessible to network participants and to the general public.

Keywords: Cultural promotion, knowledge representation, cultural mapping, ICT.

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31 Analytical Modeling of Globular Protein-Ferritin in α-Helical Conformation: A White Noise Functional Approach

Authors: Vernie C. Convicto, Henry P. Aringa, Wilson I. Barredo

Abstract:

This study presents a conformational model of the helical structures of globular protein particularly ferritin in the framework of white noise path integral formulation by using Associated Legendre functions, Bessel and convolution of Bessel and trigonometric functions as modulating functions. The model incorporates chirality features of proteins and their helix-turn-helix sequence structural motif.

Keywords: Globular protein, modulating function, white noise, winding probability.

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30 Risk Monitoring through Traceability Information Model

Authors: Juan P. Zamora, Wilson Adarme, Laura Palacios

Abstract:

This paper shows a traceability framework for supply risk monitoring, beginning with the identification, analysis, and evaluation of the supply chain risk and focusing on the supply operations of the Health Care Institutions with oncology services in Bogota, Colombia. It includes a brief presentation of the state of the art of the Supply Chain Risk Management and traceability systems in logistics operations, and it concludes with the methodology to integrate the SCRM model with the traceability system.

Keywords: Supply risk, risk monitoring, supply chain risk management, cancer drugs, traceability systems.

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29 AGV Guidance System: An Application of Simple Active Contour for Visual Tracking

Authors: M.Asif, M.R.Arshad, P.A.Wilson

Abstract:

In this paper, a simple active contour based visual tracking algorithm is presented for outdoor AGV application which is currently under development at the USM robotic research group (URRG) lab. The presented algorithm is computationally low cost and able to track road boundaries in an image sequence and can easily be implemented on available low cost hardware. The proposed algorithm used an active shape modeling using the B-spline deformable template and recursive curve fitting method to track the current orientation of the road.

Keywords: Active contour, B-spline, recursive curve fitting.

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28 Multi-board Run-time Reconfigurable Implementation of Intrinsic Evolvable Hardware

Authors: Cyrille Lambert, Tatiana Kalganova, Emanuele Stomeo, Manissa Wilson

Abstract:

A multi-board run-time reconfigurable (MRTR) system for evolvable hardware (EHW) is introduced with the aim to implement on hardware the bidirectional incremental evolution (BIE) method. The main features of this digital intrinsic EHW solution rely on the multi-board approach, the variable chromosome length management and the partial configuration of the reconfigurable circuit. These three features provide a high scalability to the solution. The design has been written in VHDL with the concern of not being platform dependant in order to keep a flexibility factor as high as possible. This solution helps tackling the problem of evolving complex task on digital configurable support.

Keywords: Evolvable Hardware, Evolutionary Strategy, multiboardFPGA system.

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27 Implementing a Database from a Requirement Specification

Authors: M. Omer, D. Wilson

Abstract:

Creating a database scheme is essentially a manual process. From a requirement specification the information contained within has to be analyzed and reduced into a set of tables, attributes and relationships. This is a time consuming process that has to go through several stages before an acceptable database schema is achieved. The purpose of this paper is to implement a Natural Language Processing (NLP) based tool to produce a relational database from a requirement specification. The Stanford CoreNLP version 3.3.1 and the Java programming were used to implement the proposed model. The outcome of this study indicates that a first draft of a relational database schema can be extracted from a requirement specification by using NLP tools and techniques with minimum user intervention. Therefore this method is a step forward in finding a solution that requires little or no user intervention.

Keywords: Information Extraction, Natural Language Processing, Relation Extraction.

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26 Grid Based and Random Based Ant Colony Algorithms for Automatic Hose Routing in 3D Space

Authors: Gishantha Thantulage, Tatiana Kalganova, Manissa Wilson

Abstract:

Ant Colony Algorithms have been applied to difficult combinatorial optimization problems such as the travelling salesman problem and the quadratic assignment problem. In this paper gridbased and random-based ant colony algorithms are proposed for automatic 3D hose routing and their pros and cons are discussed. The algorithm uses the tessellated format for the obstacles and the generated hoses in order to detect collisions. The representation of obstacles and hoses in the tessellated format greatly helps the algorithm towards handling free-form objects and speeds up computation. The performance of algorithm has been tested on a number of 3D models.

Keywords: Ant colony algorithm, Automatic hose routing, tessellated format, RAPID.

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25 Modelling the Occurrence of Defects and Change Requests during User Acceptance Testing

Authors: Kevin McDaid, Simon P. Wilson

Abstract:

Software developed for a specific customer under contract typically undergoes a period of testing by the customer before acceptance. This is known as user acceptance testing and the process can reveal both defects in the system and requests for changes to the product. This paper uses nonhomogeneous Poisson processes to model a real user acceptance data set from a recently developed system. In particular a split Poisson process is shown to provide an excellent fit to the data. The paper explains how this model can be used to aid the allocation of resources through the accurate prediction of occurrences both during the acceptance testing phase and before this activity begins.

Keywords: User acceptance testing. Software reliability growth modelling. Split Poisson process. Bayesian methods.

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24 Coordination on Agrifood Supply Chain

Authors: Martha Liliana Reina Usuga, Wilson Adarme Jaimes, Oscar Eduardo Suarez

Abstract:

Coordinated supply chain represents major challenges for the different actors involved in it, because each agent responds to individual interests. The paper presents a framework with the reviewed literature regarding the system's decision structure and nature of demand. Later, it characterizes an agri food supply chain in the Central Region of Colombia, it responds to a decentralized distribution system and a stochastic demand. Finally, the paper recommends coordinating the chain based on shared information, and mechanisms for each agent, as VMI (vendor-managed inventory) strategy for farmer-buyer relationship, information system for farmers and contracts for transportation service providers.

Keywords: Agri-food supply chain, Coordination mechanisms, Decentralized distribution system, Supply chain coordination.

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23 Isobaric Vapor-Liquid Equilibria of Mesitylene + 1- Heptanol and Mesitylene +1-Octanol at 97.3 kPa

Authors: Seema Kapoor, Sushil K. Kansal, Baljinder K. Gill, Aarti Sharma, Swati Arora

Abstract:

Isobaric vapor-liquid equilibrium measurements are reported for the binary mixtures of Mesitylene + 1-Heptanol and Mesitylene + 1-Octanol at 97.3 kPa. The measurements have been performed using a vapor recirculating type (modified Othmer's) equilibrium still. Both the mixtures show positive deviation from ideality. The Mesitylene + 1-Heptanol mixture forms an azeotrope whereas Mesitylene + 1- Octanol form a non – azeotropic mixture. The activity coefficients have been calculated taking into consideration the vapor phase nonideality. The data satisfy the thermodynamic consistency tests of Herington, and Hirata. The activity coefficients have been satisfactorily correlated by means of the Margules, Redlich-Kister, Wilson, Black, and NRTL equations. The activity coefficient values have also been obtained by UNIFAC method.

Keywords: Binary mixture, Mesitylene, Vapor-liquid equilibrium, 1-Heptanol, 1-Octanol.

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22 Gravitino Dark Matter in (nearly) SLagy D3/D7 m-Split SUSY

Authors: Mansi Dhuria, Aalok Misra

Abstract:

In the context of large volume Big Divisor (nearly) SLagy D3/D7 μ-Split SUSY [1], after an explicit identification of first generation of SM leptons and quarks with fermionic superpartners of four Wilson line moduli, we discuss the identification of gravitino as a potential dark matter candidate by explicitly calculating the decay life times of gravitino (LSP) to be greater than age of universe and lifetimes of decays of the co-NLSPs (the first generation squark/slepton and a neutralino) to the LSP (the gravitino) to be very small to respect BBN constraints. Interested in non-thermal production mechanism of gravitino, we evaluate the relic abundance of gravitino LSP in terms of that of the co-NLSP-s by evaluating their (co-)annihilation cross sections and hence show that the former satisfies the requirement for a potential Dark Matter candidate. We also show that it is possible to obtain a 125 GeV light Higgs in our setup.

Keywords: Split Supersymmetry, Large Volume Swiss-Cheese Calabi-Yau's, Dark Matter, (N)LSP decays, relic abundance.

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21 Expert Solutions to Affordable Housing Finance Challenges in Developing Economies

Authors: Timothy Akinwande, Eddie C. M. Hui

Abstract:

Housing the urban poor has remained a challenge for many years across the world, especially in developing economies, despite the apparent research attention and policy interventions. It is apt to investigate the prevalent affordable housing (AH) provision challenges using unconventional approaches. It is pragmatic to thoroughly examine housing experts to provide supply-side solutions to AH challenges and investigate informal settlers to deduce solutions from AH demand viewpoints. This study, being the supply-side investigation of an ongoing research, interrogated housing experts to determine significant expert solutions. Focus group discussions and in-depth interviews were conducted with housing experts in Nigeria. Through descriptive, content, and systematic thematic analyses of data, major findings are that deliberate finance models designed for the urban poor are the most significant housing finance solution in developing economies. Other findings are that adequately implemented rent control policies, deliberate Public-Private Partnership (PPP) approaches like inclusionary housing and land-value capture, and urban renewal programs to enlighten and tutor the urban poor on how to earn more, spend wisely, and invest in their own better housing will effectively solve AH finance challenges. Study findings are informative for the best approaches to achieve effective, AH finance for the urban poor in Nigeria, which is indispensable for the achievement of sustainable development goals. This research’s originality lies in the exploration of experts’ opinions in relation to AH finance to produce an equation model of critical solutions to AH finance challenges. Study data are useful resources for future pro-poor housing studies. This study makes housing policy-oriented recommendations toward effective, AH for the urban poor in developing countries.

Keywords: Affordable housing, effective affordable housing, housing policy, housing research, sustainable development, urban poor.

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20 Supervisory Fuzzy Learning Control for Underwater Target Tracking

Authors: C.Kia, M.R.Arshad, A.H.Adom, P.A.Wilson

Abstract:

This paper presents recent work on the improvement of the robotics vision based control strategy for underwater pipeline tracking system. The study focuses on developing image processing algorithms and a fuzzy inference system for the analysis of the terrain. The main goal is to implement the supervisory fuzzy learning control technique to reduce the errors on navigation decision due to the pipeline occlusion problem. The system developed is capable of interpreting underwater images containing occluded pipeline, seabed and other unwanted noise. The algorithm proposed in previous work does not explore the cooperation between fuzzy controllers, knowledge and learnt data to improve the outputs for underwater pipeline tracking. Computer simulations and prototype simulations demonstrate the effectiveness of this approach. The system accuracy level has also been discussed.

Keywords: Fuzzy logic, Underwater target tracking, Autonomous underwater vehicles, Artificial intelligence, Simulations, Robot navigation, Vision system.

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19 HIV Treatment Planning on a case-by-CASE Basis

Authors: Marios M. Hadjiandreou, Raul Conejeros, Ian Wilson

Abstract:

This study presents a mathematical modeling approach to the planning of HIV therapies on an individual basis. The model replicates clinical data from typical-progressors to AIDS for all stages of the disease with good agreement. Clinical data from rapid-progressors and long-term non-progressors is also matched by estimation of immune system parameters only. The ability of the model to reproduce these phenomena validates the formulation, a fact which is exploited in the investigation of effective therapies. The therapy investigation suggests that, unlike continuous therapy, structured treatment interruptions (STIs) are able to control the increase in both the drug-sensitive and drug-resistant virus population and, hence, prevent the ultimate progression from HIV to AIDS. The optimization results further suggest that even patients characterised by the same progression type can respond very differently to the same treatment and that the latter should be designed on a case-by-case basis. Such a methodology is presented here.

Keywords: AIDS, chemotherapy, mathematical modeling, optimal control, progression.

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18 Bio-inspired Audio Content-Based Retrieval Framework (B-ACRF)

Authors: Noor A. Draman, Campbell Wilson, Sea Ling

Abstract:

Content-based music retrieval generally involves analyzing, searching and retrieving music based on low or high level features of a song which normally used to represent artists, songs or music genre. Identifying them would normally involve feature extraction and classification tasks. Theoretically the greater features analyzed, the better the classification accuracy can be achieved but with longer execution time. Technique to select significant features is important as it will reduce dimensions of feature used in classification and contributes to the accuracy. Artificial Immune System (AIS) approach will be investigated and applied in the classification task. Bio-inspired audio content-based retrieval framework (B-ACRF) is proposed at the end of this paper where it embraces issues that need further consideration in music retrieval performances.

Keywords: Bio-inspired audio content-based retrieval framework, features selection technique, low/high level features, artificial immune system

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17 Application of Sensory Thermography as Measuring Method to Study Median Nerve Temperatures

Authors: Javier Ordorica Villalvazo, Claudia Camargo Wilson, Jesus Everardo Olguin Tiznado

Abstract:

This paper presents an experimental case using sensory thermography to describe temperatures behavior on median nerve once an activity of repetitive motion was done. Thermography is a noninvasive technique without biological hazard and not harm at all times and has been applied in many experiments to seek for temperature patterns that help to understand diseases like cancer and cumulative trauma disorders (CTD’s). An infrared sensory thermography technology was developed to execute this study. Three women in good shape were selected for the repetitive motion tests for 4 days, two right-handed women and 1 left handed woman, two sensory thermographers were put on both median nerve wrists to get measures. The evaluation time was of 3 hours 30 minutes in a controlled temperature, 20 minutes of stabilization time at the beginning and end of the operation. Temperatures distributions are statistically evaluated and showed similar temperature patterns behavior.

Keywords: Median nerve, temperature, sensory thermography, wrists, CTD’s.

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16 Design of Encoding Calculator Software for Huffman and Shannon-Fano Algorithms

Authors: Wilson Chanhemo, Henry. R. Mgombelo, Omar F Hamad, T. Marwala

Abstract:

This paper presents a design of source encoding calculator software which applies the two famous algorithms in the field of information theory- the Shannon-Fano and the Huffman schemes. This design helps to easily realize the algorithms without going into a cumbersome, tedious and prone to error manual mechanism of encoding the signals during the transmission. The work describes the design of the software, how it works, comparison with related works, its efficiency, its usefulness in the field of information technology studies and the future prospects of the software to engineers, students, technicians and alike. The designed “Encodia" software has been developed, tested and found to meet the intended requirements. It is expected that this application will help students and teaching staff in their daily doing of information theory related tasks. The process is ongoing to modify this tool so that it can also be more intensely useful in research activities on source coding.

Keywords: Coding techniques, Coding algorithms, Codingefficiency, Encodia, Encoding software.

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15 Modeling of Thermal Processes Associated to an Electric Arc

Authors: Allagui Hatem, Ghodbane Fathi

Abstract:

The primary objective of this paper is to study the thermal effects of the electric arc on the breaker apparatus contacts for forecasting and improving the contact durability. We will propose a model which takes account of the main influence factors on the erosion contacts. This phenomenon is very complicated because the amount of ejected metal is not necessarily constituted by the whole melted metal bath but this depends on the balance of forces on the contact surface. Consequently, to calculate the metal ejection coefficient, we propose a method which consists in comparing the experimental results with the calculated ones. The proposed model estimates the mass lost by vaporization, by droplets ejection and by the extraction mechanism of liquid or solid metal. In the one-dimensional geometry, to calculate of the contact heating, we used Green’s function which expresses the point source and allows the transition to the surface source. However, for the two- dimensional model we used explicit and implicit numerical methods. The results are similar to those found by Wilson’s experiments.

Keywords: Electric arc, thermal effect, erosion, contact, durability.

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14 A Black-box Approach for Response Quality Evaluation of Conversational Agent Systems

Authors: Ong Sing Goh, C. Ardil, Wilson Wong, Chun Che Fung

Abstract:

The evaluation of conversational agents or chatterbots question answering systems is a major research area that needs much attention. Before the rise of domain-oriented conversational agents based on natural language understanding and reasoning, evaluation is never a problem as information retrieval-based metrics are readily available for use. However, when chatterbots began to become more domain specific, evaluation becomes a real issue. This is especially true when understanding and reasoning is required to cater for a wider variety of questions and at the same time to achieve high quality responses. This paper discusses the inappropriateness of the existing measures for response quality evaluation and the call for new standard measures and related considerations are brought forward. As a short-term solution for evaluating response quality of conversational agents, and to demonstrate the challenges in evaluating systems of different nature, this research proposes a blackbox approach using observation, classification scheme and a scoring mechanism to assess and rank three example systems, AnswerBus, START and AINI.

Keywords: Evaluation, conversational agents, Response Quality, chatterbots

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13 Response Quality Evaluation in Heterogeneous Question Answering System: A Black-box Approach

Authors: Goh Ong Sing, C. Ardil, Wilson Wong, Shahrin Sahib

Abstract:

The evaluation of the question answering system is a major research area that needs much attention. Before the rise of domain-oriented question answering systems based on natural language understanding and reasoning, evaluation is never a problem as information retrieval-based metrics are readily available for use. However, when question answering systems began to be more domains specific, evaluation becomes a real issue. This is especially true when understanding and reasoning is required to cater for a wider variety of questions and at the same time achieve higher quality responses The research in this paper discusses the inappropriateness of the existing measure for response quality evaluation and in a later part, the call for new standard measures and the related considerations are brought forward. As a short-term solution for evaluating response quality of heterogeneous systems, and to demonstrate the challenges in evaluating systems of different nature, this research presents a black-box approach using observation, classification scheme and a scoring mechanism to assess and rank three example systems (i.e. AnswerBus, START and NaLURI).

Keywords: Evaluation, question answering, response quality.

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12 A Nanosensor System Based On Disuccinimydyl–CYP2E1 for Amperometric Detection of the Anti-Tuberculosis Drug, Pyrazinamide

Authors: R. F. Ajayi, U. Sidwaba, U. Feleni, S. F. Douman, E. Nxusani, L. Wilson, C. Rassie, O. Tovide, P. G. L. Baker, S. L. Vilakazi, R. Tshikhudo, E. I. Iwuoha

Abstract:

Pyrazinamide (PZA) is among the first-line pro-drugs  in the tuberculosis (TB) combination chemotherapy used to treat  Mycobacterium tuberculosis. Numerous reports have suggested that  hepatotoxicity due to pyrazinamide in patients is due to inappropriate  dosing. It is, therefore necessary to develop sensitive and reliable  techniques for determining the PZA metabolic profile of diagnosed  patients promptly and at point-of-care. This study reports the  determination of PZA based on nanobiosensor systems developed  from disuccinimidyl octanedioate modified Cytochrome P450-2E1  (CYP2E1) electrodeposited on gold substrates derivatised with  (poly(8-anilino-1-napthalene sulphonic acid) PANSA/PVP-AgNPs  nanocomposites. The rapid and sensitive amperometric PZA  detection gave a dynamic linear range of 2µM to 16µM revealing a  limit of detection of 0.044µM and a sensitivity of 1.38µA/µM. The  Michaelis-Menten parameters; KM, KM app and IMAX were calculated to  be 6.0µM, 1.41µM and 1.51x10-6 A, respectively, indicating a  nanobiosensor suitable for use in serum.

Keywords: Cytochrome P450-2E1, Disuccinimidyl octanedioate, Pyrazinamide, Tuberculosis.

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11 Prediction of Saturated Hydraulic Conductivity Dynamics in an Iowan Agriculture Watershed

Authors: Mohamed Elhakeem, A. N. Thanos Papanicolaou, Christopher Wilson, Yi-Jia Chang

Abstract:

In this study, a physically-based, modeling framework was developed to predict saturated hydraulic conductivity (Ksat) dynamics in the Clear Creek Watershed (CCW), Iowa. The modeling framework integrated selected pedotransfer functions and watershed models with geospatial tools. A number of pedotransfer functions and agricultural watershed models were examined to select the appropriate models that represent the study site conditions. Models selection was based on statistical measures of the models’ errors compared to the Ksat field measurements conducted in the CCW under different soil, climate and land use conditions. The study has shown that the predictions of the combined pedotransfer function of Rosetta and the Water Erosion Prediction Project (WEPP) provided the best agreement to the measured Ksat values in the CCW compared to the other tested models. Therefore, Rosetta and WEPP were integrated with the Geographic Information System (GIS) tools for visualization of the data in forms of geospatial maps and prediction of Ksat variability in CCW due to the seasonal changes in climate and land use activities. 

Keywords: Saturated hydraulic conductivity, pedotransfer functions, watershed models, geospatial tools.

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10 Studies on Bioaccumulation of 51Cr by Ulva sp. and Ruppia maritima

Authors: Clarissa L. de Araujo, Kátia N. Suzuki, Wilson T. V. Machado, Luis F. Bellido, Alfredo V.B. Bellido

Abstract:

This study aims at contributing to the characterization of the process of biological incorporation of chromium by two benthonic species, the macroalgae Ulva sp. and the aquatic macrophyte Ruppia maritima, to subsidize future activities of monitoring the contamination of aquatic biota. This study is based on laboratory experiments to characterize the incorporation kinetics of the radiotracer 51Cr in two oxidation states (III and VI), under different salinities (7, 15, and 21 ‰). Samples of two benthonic species were collected on the margins of Rodrigo de Freitas Lagoon (Rio de Janeiro, Brazil), acclimated in the laboratory and subsequently subjected to experiments. In tests with 51Cr (III and IV), it was observed that accumulation of the metal in Ulva sp. has inverse relationship with salinity, while for R. maritima, the maximum accumulation occurs in salinity 21‰. In experiments with Cr(III), increases in the uptake of ion by both species were verified. The activity of Cr(III) was up to 19 times greater than the Cr(VI). As regards the potential for accumulation of metals, a better sensitivity of Ulva sp. for any chromium tri or hexavalent forms was verified, while for the Cr(VI) it will require low salinities and longer exposure (>24h). For R. maritima, the results showed the uptake of Cr(VI) increase along with time (>20h), because this species is more resistant for the hexavalent form and useful for any salinity as well.

Keywords: Chromium, Cr-51, macroalgae, macrophyte, uptake.

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9 Effect of Size of the Step in the Response Surface Methodology using Nonlinear Test Functions

Authors: Jesús Everardo Olguín Tiznado, Rafael García Martínez, Claudia Camargo Wilson, Juan Andrés López Barreras, Everardo Inzunza González, Javier Ordorica Villalvazo

Abstract:

The response surface methodology (RSM) is a collection of mathematical and statistical techniques useful in the modeling and analysis of problems in which the dependent variable receives the influence of several independent variables, in order to determine which are the conditions under which should operate these variables to optimize a production process. The RSM estimated a regression model of first order, and sets the search direction using the method of maximum / minimum slope up / down MMS U/D. However, this method selects the step size intuitively, which can affect the efficiency of the RSM. This paper assesses how the step size affects the efficiency of this methodology. The numerical examples are carried out through Monte Carlo experiments, evaluating three response variables: efficiency gain function, the optimum distance and the number of iterations. The results in the simulation experiments showed that in response variables efficiency and gain function at the optimum distance were not affected by the step size, while the number of iterations is found that the efficiency if it is affected by the size of the step and function type of test used.

Keywords: RSM, dependent variable, independent variables, efficiency, simulation

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