Search results for: M. Rajasimman

Authors: M.Rajasimman, S.Subathra

Abstract:

In this work, statistical experimental design was applied for the optimization of medium constituents for Gentamicin production by Micromsonospora echinospora subs pallida (MTCC 708) in a batch reactor and the results are compared with the ANN predicted values. By central composite design, 50 experiments are carried out for five test variables: Starch, Soya bean meal, K2HPO4, CaCO3 and FeSO4. The optimum condition was found to be: Starch (8.9,g/L), Soya bean meal (3.3 g/L), K2HPO4 (0.8 g/L), CaCO3 (4 g/L) and FeSO4 (0.03 g/L). At these optimized conditions, the yield of gentamicin was found to be 1020 mg/L. The R2 values were found to be 1 for ANN training set, 0.9953 for ANN test set, and 0.9286 for RSM.

Keywords: Gentamicin, optimization, Micromonospora echinospora, ANN, RSM

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Authors: M.Rajasimman, S.Subathra

Abstract:

The process parameters, temperature, pH and substrate concentration, were optimized for the production of gentamicin using Micromonouspora echinospora. Experiments were carried out according to central composite design in response surface method. The optimum conditions for the maximum production of gentamicin were found to be: temperature – 31.7oC, pH – 6.8 and substrate concentration – 3%. At these optimized conditions the production of gentamicin was found to be – 1040 mg/L. The R2 value of 0.9465 indicates a good fitness of the model.

Keywords: Gentamicin, Micromonouspora echinospora, response surface method, optimization, central composite design.

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Authors: G.Durai, N.Rajamohan, C.Karthikeyan, M.Rajasimman

Abstract:

In this study, aerobic digestion of tannery industry wastewater was carried out using mixed culture obtained from common effluent treatment plant treating tannery wastewater. The effect of pH, temperature, inoculum concentration, agitation speed and initial substrate concentration on the reduction of organic matters were found. The optimum conditions for COD reduction was found to be pH - 7 (60%), temperature - 30ÔùªC (61%), inoculum concentration - 2% (61%), agitation speed - 150rpm (65%) and initial substrate concentration - 1560 mg COD/L (74%). Kinetics studies were carried by using Monod model, First order, Diffusional model and Singh model. From the results it was found that the Monod model suits well for the degradation of tannery wastewater using mixed microbial consortium.

Keywords: Tannery, Wastewater, Biological treatment, Aerobic, Mixed culture, Kinetics.

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Authors: M. Rajasimman, K. Murugaiyan

Abstract:

In this work, sorption of nickel from aqueous solution on hypnea valentiae, red macro algae, was investigated. Batch experiments have been carried out to find the effect of various parameters such as pH, temperature, sorbent dosage, metal concentration and contact time on the sorption of nickel using hypnea valentiae. Response surface methodology (RSM) is employed to optimize the process parameters. Based on the central composite design, quadratic model was developed to correlate the process variables to the response. The most influential factor on each experimental design response was identified from the analysis of variance (ANOVA). The optimum conditions for the sorption of nickel were found to be: pH – 5.1, temperature – 36.8oC, sorbent dosage – 5.1 g/L, metal concentration – 100 mg/L and contact time – 30 min. At these optimized conditions the maximum removal of nickel was found to be 91.97%. A coefficient of determination R2 value 0.9548 shows the fitness of response surface methodology in this work.

Keywords: Optimization, metal, Hypnea valentia, response surface methodology, red algae.

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Authors: P. Manivannan, M. Rajasimman

Abstract:

Response surface methodology was used for quantitative investigation of water and solids transfer during osmotic dehydration of beetroot in aqueous solution of salt. Effects of temperature (25 – 45oC), processing time (30–150 min), salt concentration (5–25%, w/w) and solution to sample ratio (5:1 – 25:1) on osmotic dehydration of beetroot were estimated. Quadratic regression equations describing the effects of these factors on the water loss and solids gain were developed. It was found that effects of temperature and salt concentrations were more significant on the water loss than the effects of processing time and solution to sample ratio. As for solids gain processing time and salt concentration were the most significant factors. The osmotic dehydration process was optimized for water loss, solute gain, and weight reduction. The optimum conditions were found to be: temperature – 35oC, processing time – 90 min, salt concentration – 14.31% and solution to sample ratio 8.5:1. At these optimum values, water loss, solid gain and weight reduction were found to be 30.86 (g/100 g initial sample), 9.43 (g/100 g initial sample) and 21.43 (g/100 g initial sample) respectively.

Keywords: Optimization, Osmotic dehydration, Beetroot, saltsolution, response surface methodology

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Authors: R. Rajeshkannan, M. Rajasimman, N. Rajamohan

Abstract:

In this study, the sorption of Malachite green (MG) on Hydrilla verticillata biomass, a submerged aquatic plant, was investigated in a batch system. The effects of operating parameters such as temperature, adsorbent dosage, contact time, adsorbent size, and agitation speed on the sorption of Malachite green were analyzed using response surface methodology (RSM). The proposed quadratic model for central composite design (CCD) fitted very well to the experimental data that it could be used to navigate the design space according to ANOVA results. The optimum sorption conditions were determined as temperature - 43.5oC, adsorbent dosage - 0.26g, contact time - 200min, adsorbent size - 0.205mm (65mesh), and agitation speed - 230rpm. The Langmuir and Freundlich isotherm models were applied to the equilibrium data. The maximum monolayer coverage capacity of Hydrilla verticillata biomass for MG was found to be 91.97 mg/g at an initial pH 8.0 indicating that the optimum sorption initial pH. The external and intra particle diffusion models were also applied to sorption data of Hydrilla verticillata biomass with MG, and it was found that both the external diffusion as well as intra particle diffusion contributes to the actual sorption process. The pseudo-second order kinetic model described the MG sorption process with a good fitting.

Keywords: Response surface methodology, Hydrilla verticillata, malachite green, adsorption, central composite design

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