Search results for: Length of shoot and root

Authors: Rahim Ada, Zamari Temory, Hasan Dalgiç

Abstract:

Germination and seedling responses of seven safflower seed genotypes (Dinçer, Remzibey, Black Sun2 cultivars and A19, F4, I1, J19 lines) to different salinity concentrations (0, 5, 10 and 20g l^-1) and temperatures (10 and 20^oC) evaluated in Completely Randomized Factorial Designs in Department of Field Crops of Selcuk University, Konya, Turkey. Seeds in the control (distilled water) had at 10 and 20^oC the highest germination percentage (93.88 and 94.32%), shoot length (4.60 and 8.72cm) and root length (4.27 and 6.54cm) shoot dry weight (22.37mg and 25.99mg) and root dry weight (2.22 and 2.47mg). As the salt concentration increased, values of all characters were decreased. In this experiment, in 20g l^-1 salt concentration found germination percentage (21.28 and 26.66%), shoot (1.32 and 1.35cm) and root length (1.04 and 1.10cm) shoot (8.05mg and 7.49mg) and root dry weight (0.83 and 0.98mg) at 10 and 20^oC.

Keywords: NaCl, Safflower, Temperature.

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Authors: Thitiporn Machikowa, Thanawit Kulrattanarak, Sodchol Wonprasaid

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One problem of synthetic sunflower cultivation is an erratic germination of the seeds. To improve the germination, presowing seed treatment with an ultrasound was tested. All treatments were carried out at 40 kHz frequency with the intensities of 40, 60, 80 and 100% of the ultrasonic generator total power (250 W) for the durations of 5, 10, 15 and 20 minutes. Data on seed germination percentage, seed vigor index (SVI), root and shoot lengths of seedlings were collected. The results showed that germination, SVI, root and shoot lengths of ultrasonic treated seedlings were different from the control, depending on intensity of the ultrasound. The effects of ultrasonic treatment were significant on germination, resulting in a maximum increase of 43% at 40 and 60% intensities compared to that of the control seeds. In addition, seedlings of these 2 treatments had higher SVI and longer root and shoot lengths than that of the control seedlings. All treatment durations resulted in higher germination and SVI, longer root and higher shoot lenghts of seedlings than the control. Among the duration treatments, only SVI and seedling root length were significantly different.

Keywords: Ultrasonic, germination, root length, shoot length, Helianthus annuus L.

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Authors: M. Soltani Howyzeh, N. Kardoni, M. Mojadam

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To evaluate the vigor of wheat seeds and stress of premature aging effects on germination percentage, root length and shoot length of five wheat cultivars that include Vynak, Karkheh, Chamran, Star and Kavir which underwent a period of zero, two, three, four days in terms of premature aging with 41°C temperature and 100% relative humidity. Seed germination percentage, root length and shoot length in these conditions were measured. This experiment was conducted as a factorial completely randomized design with four replications in laboratory conditions. The results showed that each of aging treatments used in this experiment can be used to detect differences in vigor of wheat varieties. Wheat cultivars illustrated significant differences in germination percentage, root length and shoot length in terms of premature aging. The wheat cultivars; Astar and Vynak had maximum germination percentage and Karkheh, respectively Kavir and Chamran had lowest percentage of seed germination. Reactions of root and shoot length of wheat cultivars was also different. The results showed that the seeds with a stronger vigor affected less in premature aging condition and the difference between the percentage of seed germination under normal conditions and stress was significant and the seeds with the weaker vigor were more sensitive to the premature aging stress and the premature aging had more severe negative impact on seed vigor.

Keywords: Wheat cultivars, seed vigor, premature aging effects.

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Authors: Satyendra Nath Mandal, J. Pal Choudhury, Dilip De, S. R. Bhadra Chaudhuri

Abstract:

The production of a plant can be measured in terms of seeds. The generation of seeds plays a critical role in our social and daily life. The fruit production which generates seeds, depends on the various parameters of the plant, such as shoot length, leaf number, root length, root number, etc When the plant is growing, some leaves may be lost and some new leaves may appear. It is very difficult to use the number of leaves of the tree to calculate the growth of the plant.. It is also cumbersome to measure the number of roots and length of growth of root in several time instances continuously after certain initial period of time, because roots grow deeper and deeper under ground in course of time. On the contrary, the shoot length of the tree grows in course of time which can be measured in different time instances. So the growth of the plant can be measured using the data of shoot length which are measured at different time instances after plantation. The environmental parameters like temperature, rain fall, humidity and pollution are also play some role in production of yield. The soil, crop and distance management are taken care to produce maximum amount of yields of plant. The data of the growth of shoot length of some mustard plant at the initial stage (7,14,21 & 28 days after plantation) is available from the statistical survey by a group of scientists under the supervision of Prof. Dilip De. In this paper, initial shoot length of Ken( one type of mustard plant) has been used as an initial data. The statistical models, the methods of fuzzy logic and neural network have been tested on this mustard plant and based on error analysis (calculation of average error) that model with minimum error has been selected and can be used for the assessment of shoot length at maturity. Finally, all these methods have been tested with other type of mustard plants and the particular soft computing model with the minimum error of all types has been selected for calculating the predicted data of growth of shoot length. The shoot length at the stage of maturity of all types of mustard plants has been calculated using the statistical method on the predicted data of shoot length.

Keywords: Fuzzy time series, neural network, forecasting error, average error.

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Authors: Togzhan D. Mukasheva, Anel A. Omirbekova, Raikhan S. Sydykbekova, Ramza Zh. Berzhanova, Lyudmila V. Ignatova

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In studying the possibility of using plants as rhizoremediators, barley and grass mixture which showed resistance to various concentrations of oil were selected. The minimum inhibitory effect of oil on these plants by morphological parameters such as survival of plants, length and biomass of shoot and root compared with the control was showed. In determining physiological parameters, a slight decrease in the number of chlorophyll a and b in the leaves of plants was noted. The differences in the ratio of the total surface of the roots to the work surface with the growth of plants in soil with oil in the study of adsorption of the root surface were showed.

Keywords: Length of shoot and root, biomass, chlorophyll a and b, adsorption surface, barley, grass mixture.

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Authors: Okafor C. Uche, Agbo P. Ejiofor, Okezie C. Eziuche

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This study provides a regeneration protocol for Treculia africana Decne (an endangered plant) through embryo culture. Mature zygotic embryos of T. africana were excised from the seeds aseptically and cultured on varied strengths (full, half and quarter) of Murashige and Skoog (MS) basal medium supplemented. All treatments experienced 100±0.00 percent sprouting except for half and quarter strengths. Plantlets in MS full strength had the highest fresh weight, leaf area, and longest shoot length when compared to other treatments. All explants in full, half, quarter strengths and control had the same number of leaves and sprout rate. Between the treatments, there was a significant difference (P>0.05) in their effect on the length of shoot and root, number of adventitious root, leaf area, and fresh weight. Full strength had the highest mean value in all the above-mentioned parameters and differed significantly (P>0.05) from others except in shoot length, number of adventitious roots, and root length where it did not differ (P<0.05) from half strength. The result of this study indicates that full strength MS basal medium offers a better option for the optimum growth for Treculia africana regeneration in vitro.

Keywords: Medium strengths, Murashige and Skoog, Treculia africana, zygotic embryos.

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Authors: M. E. Ghobadi, M. Ghobadi

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Waterlogging reduces shoot and root growth and final yield of wheat. Waterlogged sites have a combination of low slope, high rainfall, heavy texture and low permeability. This study was aimed the importance of waterlogging on root growth and wheat yield. In order to study the effects of different waterlogging duration (0, 10, 20 and 30 days) at growth stages (1-leaf stage, tillering stage and stem elongation stage) on root growth of wheat cultivars (Chamran, Vee/Nac and Yavaroos), one pot experiment was carried out. The experiment was a factorial according to a RCBD with three replications. Results showed that root dry weight and total root length in the anthesis and grain ripening stages and biological and grain yields were significantly different between cultivars, growth stages and waterlogging durations. Vee/Nac was found superior with respect to other cultivars. Susceptibility to waterlogging at different growth stages for cultivars was 1-leaf stage > tillering stage > stem elongation stage. Under waterlogging treatments, grain and biological yields, were decreased 44.5 and 39.8%, respectively. Root length and root dry weight were reduced 55.1 and 45.2%, respectively, too. In this experiment, decrease at root growth because of waterlogging reduced grain and biological yields. Based on the results, even short period (10 days) of waterlogging had unrecoverable effects on the root growth and grain yield of wheat.

Keywords: Wheat, waterlogging, root length, root dry weight, grain yield.

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Authors: Aree Thongpukdee, Ekasit Nisayan, Chockpisit Thepsithar

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Shoots, with three leaves, of Paphiopedilum 'Delrosi' were used as explants for multiple shoot induction. Modified Hyponex medium was supplemented with thidiazuron (TDZ), N6- benzyladenine (BA) or kinetin (Kn) alone and in combinations with 2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured for 15 weeks. It was found that TDZ alone at the concentration of 0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in combination with 13.56μM 2,4-D promoted multiple shoots. The highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh weight, height, numbers of leaf and root of new shoots and initial explants were discussed.

Keywords: Paphiopedilum, terrestrial orchids, in vitro culture, micropropagation, multiple shoot induction

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Authors: Satyendra Nath Mandal, J. Pal Choudhury, Dilip De, S. R. Bhadra Chaudhuri

Abstract:

The selection for plantation of a particular type of mustard plant depending on its productivity (pod yield) at the stage of maturity. The growth of mustard plant dependent on some parameters of that plant, these are shoot length, number of leaves, number of roots and roots length etc. As the plant is growing, some leaves may be fall down and some new leaves may come, so it can not gives the idea to develop the relationship with the seeds weight at mature stage of that plant. It is not possible to find the number of roots and root length of mustard plant at growing stage that will be harmful of this plant as roots goes deeper to deeper inside the land. Only the value of shoot length which increases in course of time can be measured at different time instances. Weather parameters are maximum and minimum humidity, rain fall, maximum and minimum temperature may effect the growth of the plant. The parameters of pollution, water, soil, distance and crop management may be dominant factors of growth of plant and its productivity. Considering all parameters, the growth of the plant is very uncertain, fuzzy environment can be considered for the prediction of shoot length at maturity of the plant. Fuzzification plays a greater role for fuzzification of data, which is based on certain membership functions. Here an effort has been made to fuzzify the original data based on gaussian function, triangular function, s-function, Trapezoidal and L –function. After that all fuzzified data are defuzzified to get normal form. Finally the error analysis (calculation of forecasting error and average error) indicates the membership function appropriate for fuzzification of data and use to predict the shoot length at maturity. The result is also verified using residual (Absolute Residual, Maximum of Absolute Residual, Mean Absolute Residual, Mean of Mean Absolute Residual, Median of Absolute Residual and Standard Deviation) analysis.

Keywords: Fuzzification, defuzzification, gaussian function, triangular function, trapezoidal function, s-function, , membership function, residual analysis.

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Authors: E. Neamatollahi, M. Bannayan, A. Souhani Darban, A. Ghanbari

Abstract:

In production of medicinal plants, seed germination is very important problem. The treated seeds (control, hydro priming and ZnSO4) of Cumin (Cuminum cyminum L.) were evaluated at germination and seedling growth for tolerance to salt (NaCl and Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, - 0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. The objective of the study was to determine factors responsible for germination and early seedling growth due to salt toxicity or osmotic effect and to optimize the best priming treatment for these stress conditions. Results revealed that germination delayed in both solutions, having variable germination with different priming treatments. Germination, shoot and weight, root and shoot length were higher but mean germination time and abnormal germination percentage were lower in NaCl than Na2SO4 at the same water potential. The root / shoot weight and R/S length increased with increase in osmotic potential in both NaCl and Na2SO4 solutions. NaCl had less inhibitor effect on seedling growth than the germination. It was concluded that inhibition of germination at the same water potential of NaCl and Na2SO4 resulted from salt toxicity rather than osmotic effect. Hydro priming increased germination and seedling growth under salt stress. This protocol has practical importance and could be recommended to farmers to achieve higher germination and uniform emergence under field conditions.

Keywords: Priming, Germination, Nacl, ZnSo4, Na2So4.

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Authors: Maher J. Tadros, Naji K. Al-Mefleh

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The evaluation of non-conventional water resources on seed germination and seedling growth performance at early growth stages is still in progress especially in forage crops. This study was designed to test the effect of four types of water qualities (treated wastewater (TWW), industrial water (IW), grey water (GW), and Distilled water (DW)) on germination and early seedling vigor of Leucaena leucocephala. The results showed that the germination was not significantly affected by the different water qualities. Seed germination reached maximum after 17, 14, 14, and 21 days under GW, IW, TWW, and DW treatments, respectively. The highest mean of shoot length was scored under the GW treatment. And, the highest mean of root length was scored under DW which was not significant from GW treatment. The means of shoot fresh was the highest under the TWW. The means of root fresh weight was not significantly different from each other's under different treatments. The growth performance was in progress with no mortality during 21 days of growth. Thus, the best non-conventional water qualities alternatives based on the cleanness, nutrients, and toxicity are the GW, TWW and IW, respectively.

Keywords: Seed germination, Growth performance, Leucaena, Multipurpose forest trees, Waste water, Grey water

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Authors: O. P. Vinocha , J. S. Bhullar , Manish Gupta

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We considered repeated-root cyclic codes whose block length is divisible by the characteristic of the underlying field. Cyclic self dual codes are also the repeated root cyclic codes. It is known about the one-level squaring construction for binary repeated root cyclic codes. In this correspondence, we introduced of two level squaring construction for binary repeated root cyclic codes of length 2a b , a > 0, b is odd.

Keywords: Squaring Construction, generator matrix, selfdual codes, cyclic codes, coset codes, repeated root cycliccodes.

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Authors: Raheleh Khademian, Roghayeh Aminian

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For the study of salinity stress on Vicia villosa and calcium effect for modulation of that, an experiment was conducted under hydroponic condition, and some important morphological and physiological characteristics were evaluated. This experiment was conducted as a factorial based on randomized complete design with three replications. The treatments include salinity stress in three levels (0, 50, and 100 mM NaCl) and calcium in two levels (content in Hoagland solution and double content). The results showed that all morphological and physiological traits include root and shoot length, root and shoot wet and dry weight, leaf area, leaf chlorophyll content, RWC, CMS, and biological yield was significantly different from the control and is affected by the salinity stress severely. But, calcium effect on them was not significant despite of decreasing salinity effect.

Keywords: Vicia villossa, salinity stress, calcium, hydroponic.

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Authors: Sarma. M.V.R.K, Saharan. K, Prakash. A, Bisaria. V. S, Sahai.V

Abstract:

Vermiculite was used to develop inorganic carrier-based formulations of fluorescent pseudomonad strains R62 and R81. The effect of bio-inoculation of fluorescent pseudomonad strains R62 and R81 (plant growth promoting and biocontrol agent) on growth responses of Vigna-mungo under field condition was enumerated. The combined bioinoculation of these two organisms in a formuation increased the pods yield by 300% in comparison to the control crop. There was also significant increment in the other plant growth responses such as dry root weight, dry shoot weight, shoot length and number of branches per plant.

Keywords: Bio-inoculants formulation, Fluorescent pseudomonad, Plant growth promotion activity.

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Authors: Iriawati, R. R. Esyanti, W. Natalia, N. Zahya

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In vitro plant regeneration has been successfully obtained from basal shoot explant of Vetiveria zizanioides through indirect organogenesis. The explant was cultured in Murashige & Skoog’s (MS) media supplemented with 2,4-D, IAA, and kinetin in various concentrations. Callus was well induced in media supplemented with 2 ppm 2,4-D, 1 ppm IAA, and 1 ppm kinetin. This callus was then transferred to MS media supplemented with 1 - 5 ppm of BAP for shoot regeneration. The media supplemented with 3 ppm BAP was a suitable medium for shoot induction, as well as for shoot multiplication. Rooting was well developed in shoot following transferred to half MS media containing 0.2 ppm IBA. Plantlet was then transferred to husk charcoal for acclimatization, and almost all (90%) of plantlets were survived during acclimatization.

Keywords: Callus, plantlet regeneration, shoot induction, Vetiveria zizanioides.

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Authors: Zheng Zhang, Xin Chen, Guoqing Ding

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Electronic apex locators (EAL) has been widely used clinically for measuring root canal working length with high accuracy, which is crucial for successful endodontic treatment. In order to maintain high accuracy in different measurement environments, this study presented a system for root canal length measurement based on multifrequency impedance method. This measuring system can generate a sweep current with frequencies from 100 Hz to 1 MHz through a direct digital synthesizer. Multiple impedance ratios with different combinations of frequencies were obtained and transmitted by an analog-to-digital converter and several of them with representatives will be selected after data process. The system analyzed the functional relationship between these impedance ratios and the distance between the file and the apex with statistics by measuring plenty of teeth. The position of the apical foramen can be determined by the statistical model using these impedance ratios. The experimental results revealed that the accuracy of the system based on multifrequency impedance ratios method to determine the position of the apical foramen was higher than the dual-frequency impedance ratio method. Besides that, for more complex measurement environments, the performance of the system was more stable.

Keywords: Root canal length, apex locator, multifrequency impedance, sweep frequency.

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Authors: Alireza Dadkhah, Mahmoud Ghorbanzadeh- Neghab

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This study carried out in order to investigate the effects of salinity on carbon isotope discrimination (Δ) of shoots and roots of four sugar beet cultivars (cv) including Madison (British origin) and three Iranian culivars (7233-P12, 7233-P21 and 7233-P29). Plants were grown in sand culture medium in greenhouse conditions. Plants irrigated with saline water (tap water as control, 50 mM, 150 mM, 250 mM and 350 mM of NaCl + CaCl2 in 5 to 1 molar ratio) from 4 leaves stage for 16 weeks. Carbon isotope discrimination significantly decreased with increasing salinity. Significant differences of Δ between shoot and root were observed in all cvs and all levels of salinity. Madison cv showed lower Δ in shoot and root than other three cvs at all levels of salinity expect control, but cv 7233-P29 had significantly higher Δ values at saline conditions of 150 mM and above. Therefore, Δ might be applicable, as a useful tool, for study of salinity tolerance of sugar beet genotypes.

Keywords: Carbon isotope discrimination, Photosynthesis, Salt stress, Sugar beet

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Authors: Arshad Javaid, Syeda F. Naqvi

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Macrophomina phaseolina is a devastating soil-borne fungal plant pathogen that causes charcoal rot disease in many economically important crops worldwide. So far, no registered fungicide is available against this plant pathogen. This study was planned to examine the antifungal activity of an allelopathic grass Cenchrus pennisetiformis (Hochst. & Steud.) Wipff. for the management of M. phaseolina isolated from cowpea [Vigna unguiculata (L.) Walp.] plants suffering from charcoal rot disease. Different parts of the plants viz. inflorescence, shoot and root were extracted in methanol. Laboratory bioassays were carried out using different concentrations (0, 0.5, 1.0, …, 3.0 g mL-1) of methanolic extracts of the test allelopathic grass species to assess the antifungal activity against the pathogen. In general, extracts of all parts of the grass exhibited antifungal activity. All the concentrations of methanolic extracts of shoot and root significantly reduced fungal biomass by 20–73% and 40–80%, respectively. Methanolic shoot extract was fractionated using n-hexane, chloroform, ethyl acetate and n-butanol. Different concentrations of these fractions (3.125, 6.25, …, 200 mg mL-1) were analyzed for their antifungal activity. All the concentrations of n-hexane fraction significantly reduced fungal biomass by 15–96% over corresponding control treatments. Higher concentrations (12.5–200 mg mL-1) of chloroform, ethyl acetate and n-butanol also reduced the fungal biomass significantly by 29–100%, 46–100% and 24–100%, respectively.

Keywords: Antifungal activity, Cenchrus pennisetiformis, Macrophomina phaseolina, natural fungicides

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Authors: Irfan Ahmad, Tahir Siddiqui, Rashid Ahmad Khan, Tahir Munir Butt

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An effort to find out the smaller size of cuttings for propagation of Morus alba was made in experimental area Department of Forestry, Range Management and Wildlife, University of Agriculture, Faisalabad, Pakistan. Different size of cuttings i.e. 2", 4", 6" and 8" were planted in polythene tubes of 3.5"x7". The effort was also made to compare the performance of cuttings in open air and in polythene low tunnel. Root length, number of root branches, root diameter and root fresh and dry weight were found maximum in two inches cuttings while minimum in four inches cuttings. Root growth was found maximum in open air as compared to under polythene sheet.

Keywords: cutting sizes Morus alba, Open air and polythene sheet, root growth

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Authors: Masoud Rafiee, Fatemeh Abdipoor, Hosain Lari

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An attempt was made to study of nitrogen components response of corn (Zea mays L.) to drought stress. A farm research was done in RCBD as split-plot with four replications in Khorramabad, west Iran. Drought stress levels as irrigation regimes after 75 (control), 100, and 120 (stress) mm cumulative evaporation were in main plots, and four seed corn varieties include 500 (medium maturity), 647, 700, and 704 (long maturity) were as subplots. Soluble protein, nitrate and proline amino acid were measured in shoot and root at flowering stage, and grain yield was measured in harvesting stage. As the drought progressed, the amount of nitrate and proline followed an increasing trend, but soluble protein decreased in shoot and root. The highest amount of nitrate and proline was observed in longer maturity varieties than shorter ones, but decrease yield of long maturity varieties was higher than medium maturity varieties in drought condition, because of long duration of stress.

Keywords: Nitrate, Proline, Soluble protein, Yield

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Authors: N. Rezvani, A. Sorooshzadeh, N. Farhadi

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Saffron (Crocus sativus) is cultivated as spices, medicinal and aromatic plant species. At autumn season, heavy rainfall can cause flooding stress and inhibits growth of saffron. Thus this research was conducted to study the effect of silver ion (as an ethylene inhibitor) on growth of saffron under flooding conditions. The corms of saffron were soaked with one concentration of nano silver (0, 40, 80 or 120 ppm) and then planting under flooding stress or non flooding stress conditions. Results showed that number of roots, root length, root fresh and dry weight, leaves fresh and dry weight were reduced by 10 day flooding stress. Soaking saffron corms with 40 or 80 ppm concentration of nano silver rewarded the effect of flooding stress on the root number, by increasing it. Furthermore, 40 ppm of nano silver increased root length in stress. Nano silver 80 ppm in flooding stress, increased leaves dry weight.

Keywords: Flooding stress, Nano-silver, Saffron.

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Authors: Chockpisit Thepsithar, Nongnuch Euawong, Nukul Jonghomkajorn

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The in vitro culture procedure of purple nutsedge (Cyperus rotundus L.) for multiple shoot induction and tuber formation was established. Multiple shoots were significantly induced from a single shoot of about 0.5 – 0.8 cm long, on Murashige and Skoog (MS) medium supplemented with 4.44 μM 6- benzyladinine (BA) alone or in combination with 2.85 μM 1- indoleacetic acid (IAA), providing 17.6 and 15.3 shoots per explant with 31.2 and 27.5 leaves per explant, respectively, within 6 weeks of culturing. Moreover, MS medium supplemented with 4.44 μM BA and 2.85 μM IAA was suitable for tuber induction, obtaining 5.9 tubers with 3.4 rhizomes per explant. In combination with ancymidol and higher concentration of sucrose, 11.1 μM BA and 60 g/L sucrose or 11.1 μM BA, 7.8 μM ancymidol and 60 g/L sucrose induced 3.5 tubers with 1.6 rhizomes or 3.5 tubers without rhizome, respectively. However, MS medium containing 3.9 or 7.8 μM ancymidol in combination with either 60 or 80 g/L sucrose enchanced significant root formation at 20.9 – 23.6 roots per explant.

Keywords: Purple nutsedge, Cyperus rotundus, multiple shoot induction, tuber formation

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Authors: A. Özköse

Abstract:

This study was conducted to determination of yield and yield components of some fodder beet types (Amarilla Barres, Feldherr, Kyros, Magnum ve Rota) under the Konya region conditions. Fodder beet was obtained from the Selcuk University, Faculty of Agriculture, at 2006-2007 season and the experiment was established in a randomized complete block design with three replicates. Differences among the averages of the fodder beet cultivars are statistically important in terms of all the characteristics investigated. Leaf attitude value was 1.2 – 2.2 (1=erect; 5= prostrate), root shape scale value was (1=spheroidal – 9=cylindrical), root diameter 11.0 – 12.2cm, remaining part of root on the ground was 6.3 – 13.7cm, root length was 21.4 – 29.6cm, leaf yield 1592 – 1917 kg/da, root yield was 10083 – 12258 kg/da, root dry matter content was %8.2 – 18.6 and root dry matter yield was 889 – 1887 kg/da. As a result of the study, it was determined that fodder beet cultivars are different conditions in terms of yield and yield components. Therefore, determination of appropriate cultivars for each region affect crop yield importantly.

Keywords: Fodder beet, root yield, yield components.

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Authors: Hamed Zakikhani, Mohd Khanif Yusop, Amin Soltangheisi

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A hydroponic trial was carried out to investigate the effect of molybdenum (Mo) on uptake of phosphorus (P) in different rice cultivars. The experiment was conducted using a randomized complete-block design, with a split-plot arrangement of treatments and three replications. Four rates of Mo (0, 0.01, 0.1 and 1 mg L⁻¹) and five cultivars (MR219, HASHEMI, MR232, FAJRE and MR253) provided the main and sub-plots, respectively. Interaction of molybdenum×variety was significant on shoot phosphorus uptake (p≤0.01). Highest and lowest shoot phosphorus uptake were seen in Mo₃V₃ (0.6% plant^-1) and Mo₀V₃ (0.14% plant^-1) treatments, respectively. Molybdenum did not have a significant effect on root phosphorus content. According to results, application of molybdenum has a synergistic effect on uptake of phosphorus by rice plants.

Keywords: Molybdenum, Phosphorus, Uptake, rice.

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Authors: Pankaj Singh Rawat, Rajeew Kumar, Pradeep Ram, Priyanka Pandey

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Wheat is an important cereal crop for food security. Boosting the wheat production and productivity is the major challenge across the nation. Good quality of seed is required for maintaining optimum plant stand which ultimately increases grain yield. Ensuring a good germination is one of the key steps to ensure proper plant stand and moisture assurance during seed germination may help to speed up the germination. The tiny size of nanoparticles may help in entry of water into seed without disturbing their internal structure. Considering above, a laboratory experiment was conducted during 2012-13 at G.B. Pant University of Agriculture and Technology, Pantnagar, India. The completely randomized design was used for statistical analysis. The experiment was conducted in two phases. In the first phase, the appropriate concentration of nanoparticles for seed treatment was screened. In second phase seed soaking hours of nanoparticles for better seed germination were standardized. Wheat variety UP2526 was taken as test crop. Four nanoparticles (TiO₂, ZnO, nickel and chitosan) were taken for study. The crop germination studies were done in petri dishes and standard package and practices were used to raise the seedlings. The germination studies were done by following standard procedure. In first phase of the experiment, seeds were treated with 50 and 300 ppm of nanoparticles and control was also maintained for comparison. In the second phase of experiment, seeds were soaked for 4 hours, 6 hours and 8 hours with 50 ppm nanoparticles of TiO₂, ZnO, nickel and chitosan along with control treatment to identify the soaking time for better seed germination. Experiment revealed that the application of nanoparticles help to enhance seed germination. The study revealed that seed treatment with  nanoparticles at 50 ppm concentration increases root length, shoot length, seedling length, shoot dry weight, seedling dry weight, seedling vigour index I and seedling vigour index II as compared to seed soaking at 300 ppm concentration. This experiment showed that seed soaking up to 4 hr was better as compared to 6 and 8 hrs. Seed soaking with nanoparticles specially TiO₂, ZnO, and chitosan proved to enhance germination and seedling growth indices of wheat crop.

Keywords: Nanoparticles, seed germination, seed soaking, wheat.

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Authors: K. Uteulin, S. Mukhambetzhanov, I. Rakhimbaev

Abstract:

This experiment was performed to optimize the medium for tissue culture of Taraxacum kok-saghyz Rodin. Different tissue culture approaches such as shoot regeneration from seed, callus formation from leaf explants and plant regeneration from callus were investigated in this study. All the explants were cultured on MS basal medium supplemented with 20g/l sucrose, 7g/l agar and different plant growth regulators. Seeds of Taraxacum kok-saghyzwere cultured on media containing different levels of BA and 2,4-D (0.5, 1.0 and 3.0mg/L) to direct shoot regeneration study. Leaf explants were cultured in different combination of BA (at three levels: 0.5, 1.0 and 3.0mg/L) and zeatin (at two levels: 0.5 and 1.0mg/L) to examine callus formation. After the callus formation the formed calli were cultured on different combinations of BA and NAA for shoot regeneration. BA at three levels (0.5 and 1.0 and 3.0mg/L) and NAA at two levels (0.5 and 1.0mg/L) in all possible combinations were used for shoot regeneration from callus. The results showed that the treatment containing 1.0mg/L 2,4-D in combination with 1.0mg/L BA was found to be the best one for shoot regeneration from seeds. The treatment with 1.0mg/L BA in combination with 1.0mg/L zeatin were found to be suitable treatments for callus production from leaf explants, as well. Moreover, 0.5mg/L BA alone or in combination with 1.0mg/L NAA were found to be the best treatments for shoot regeneration from callus.

Keywords: Taraxacum kok-saghyz Rodin., shoot regeneration, callus.

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Authors: Charlotte C. Shagol, Tongmin Sa

Abstract:

One of the major pollutants in the environment is arsenic (As). Due to the toxic effects of As to all organisms, its remediation is necessary. Conventional technologies used in the remediation of As contaminated soils are expensive and may even compromise the structure of the soil. An attractive alternative is phytoremediation, which is the use of plants which can take up the contaminant in their tissues. Plant growth promoting bacteria (PGPB) has been known to enhance growth of plants through several mechanisms such as phytohormone production, phosphate solubilization, siderophore production and 1-aminocyclopropane-1- carboxylate (ACC) deaminase production, which is an essential trait that aids plants especially under stress conditions such as As stress. Twenty one bacteria were isolated from As-contaminated soils in the vicinity of the Janghang Smelter in Chungnam Province, South Korea. These exhibited high tolerance to either arsenite (As III) or arsenate (As V) or both. Most of these isolates possess several plant growth promoting traits which can be potentially exploited to increase phytoremediation efficiency. Among the identified isolates is Pseudomonas sp. JS1215, which produces ACC deaminase, indole acetic acid (IAA), and siderophore. It also has the ability to solubilize phosphate. Inoculation of JS1215 significantly enhanced root and shoot length and biomass accumulation of maize under normal conditions. In the presence of As, particularly in lower As level, inoculation of JS1215 slightly increased root length and biomass. Ethylene increased with increasing As concentration, but was reduced by JS1215 inoculation. JS1215 can be a potential bioinoculant for increasing phytoremediation efficiency.

Keywords: As-tolerant bacteria, plant growth promoting bacteria, As stress, phytoremediation.

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Authors: Sathida Phonoy, Bongotrut Pitiyont, Vichien kitpreechavanich

Abstract:

Sugarcane Shoots is an abundantly available residual resources consisting of lignocelluloses which take it into the benefit. The present study was focused on utilizing of sugarcane shoot for reducing sugar production as a substrate in ethanol production. Physical and chemical pretreatments of sugarcane shoot were investigated. Results showed that the size of sugarcane shoot influenced the cellulose content. The maximum cellulose yield (60 %) can be obtained from alkaline pretreated sugarcane shoot with 1.0 M NaOH at 30 oC for 90 min. The cellulose yield reached up to 93.9% (w/w). Enzymatically hydrolyzed of cellulosic residual in 0.04 citrate buffer (pH 5) with celluclast 1.5L (0.7 FPU/ml) resulted in the highest amount of reducing sugar at a rate of 32.1 g/l after 4 h incubation at 50°C, and 100 oC for 5 min . Cellulose conversion was 55.5%.

Keywords: Conversion, Sugarcane Shoots, Reducing Sugars.

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Authors: Luh Putu T. Darmayanti, A. A. Duwipayana, I Nengah K. Putra, Nyoman S. Antara

Abstract:

Processing tabah bamboo shoot as fermented pickle is one of the way to increase the shelf life of this bamboo shoot. The advantage of this shoot is low concentration of hydro cyanic acid (HCN) make it potential for functional food product. This study aimed to determine the characteristic of tabah bamboo shoot pickle such as total of lactic acid bacteria (LAB), pH, total acidity, and hydro cyanic acid (HCN) content, and also find the LAB’s type involved during fermentation, and organic acids’ profiles. The pickle was made by natural fermentation with 6% salt concentration and fermentation conducted for 13 days. The result showed during the fermentation time, in the 4th day LAB’s number was highest as much as 72 x 107 CFU/ml and the lowest pH was 3.09. We also found decreasing in HCN from 37.8 ppm at the beginning to 20.52 ppm at the end of fermentation process. The organic acids detected during the fermentation were lactic acid with the highest concentration was 0.0546 g/100 g and small amount of acetic acid. By using PCR method, the 18 of LABs which had rod shape were detected as member of Lactobacillus spp., in which 17 strains detected as L. plantarum.

Keywords: Fermentation, LAB, pickle, tabah bamboo shoot.

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Authors: M. Hajzler, J. Klimesova, T. Streda, K. Vejrazka, V. Marecek, T. Cholastova

Abstract:

The most planted cover crops in the Czech Republic are mustard (Sinapis alba) and phacelia (Phacelia tanacetifolia Benth.). A field trial was executed to evaluate root system size (RSS) in eight varieties of mustard and five varieties of phacelia on two locations, in three BBCH phases and in two years. The relationship between RSS and aboveground biomass was inquired. The root system was assessed by measuring its electric capacity. Aboveground mass and root samples to be evaluated by means of a digital image analysis were recovered in the BBCH phase 70. The yield of aboveground biomass of mustard was always statistically significantly higher than that of phacelia. Mustard showed a statistically significant negative correlation between root length density (RLD) within 10 cm and aboveground biomass weight (r = - 0.46*). Phacelia featured a statistically significant correlation between aboveground biomass production and nitrate nitrogen content in soil (r=0.782**).

Keywords: Aboveground Biomass, Cover crop, Nitrogen content, Root system size

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