Search results for: Eyring%20Accelerated%20Model

2 Appling Eyring-s Accelerated Life Testing Model to “Times to Breakdown“ of Insulating Fluid: A Combined Approach of an Accelerated and a Sequential Life Testing

Authors: D. I. De Souza, D. R. Fonseca, D. Kipper

Abstract:

In this paper, the test purpose will be to assess whether or not the accelerated model proposed by Eyring will be able to translate results for the shape and scale parameters of an underlying Weibull model, obtained under two accelerating using conditions, to expected normal using condition results for these parameters. The product being analyzed is a new type of insulate fluid, and the accelerating factor is the voltage stresses applied to the fluid at two different levels (30KV and 40KV). The normal operating voltage is 25KV. In this case, it was possible to test the insulate fluid at normal voltage using condition. Both results for the two parameters of the Weibull model, obtained under normal using condition and translated from accelerated using conditions to normal conditions, will be compared to each other to assess the accuracy of the Eyring model when the accelerating factor is only the voltage stress.

Keywords: Eyring Accelerated Model, Sequential Life Testing, Two-Parameter Weibull Distribution, Voltage Stresses.

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1 Comparing Spontaneous Hydrolysis Rates of Activated Models of DNA and RNA

Authors: Mohamed S. Sasi, Adel M. Mlitan, Abdulfattah M. Alkherraz

Abstract:

This research project aims to investigate difference in relative rates concerning phosphoryl transfer relevant to biological catalysis of DNA and RNA in the pH-independent reactions. Activated Models of DNA and RNA for alkyl-aryl phosphate diesters (with 4-nitrophenyl as a good leaving group) have successfully been prepared to gather kinetic parameters. Eyring plots for the pH– independent hydrolysis of 1 and 2 were established at different temperatures in the range 100–160 °C. These measurements have been used to provide a better estimate for the difference in relative rates between the reactivity of DNA and RNA cleavage. Eyring plot gave an extrapolated rate of kH2O = 1 × 10-10 s -1 for 1 (RNA model) and 2 (DNA model) at 25°C. Comparing the reactivity of RNA model and DNA model shows that the difference in relative rates in the pH-independent reactions is surprisingly very similar at 25°. This allows us to obtain chemical insights into how biological catalysts such as enzymes may have evolved to perform their current functions.

Keywords: DNA & RNA Models, Relative Rates, Reactivity.

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