Search results for: LDL Surface Concentration (LSC)

Authors: D. Vainauska, S. Kozireva, A. Karpovs, M. Chistyakovs, M. Baryshev

Abstract:

Liposomal magnetofection is a simple, highly efficient technology for cell transfection, demonstrating better outcome than a number of other common gene delivery methods. However, aggregate complexes distribution over the cell surface is non-uniform due to the gradient of the permanent magnetic field. The aim of this study was to estimate the efficiency of liposomal magnetofection for prostate carcinoma PC3 cell line using newly designed device, “DynaFECTOR", ensuring magnetofection in a dynamic gradient magnetic field. Liposomal magnetofection in a dynamic gradient magnetic field demonstrated the highest transfection efficiency for PC3 cells – it increased for 21% in comparison with liposomal magnetofection and for 42% in comparison with lipofection alone. The optimal incubation time under dynamic magnetic field for PC3 cell line was 5 minutes and the optimal rotation frequency of magnets – 5 rpm. The new approach also revealed lower cytotoxic effect to cells than liposomal magnetofection.

Keywords: Dynamic gradient magnetic field, gene delivery, liposomal magnetofection, prostate cancer cell line

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Authors: Jin Sun Oh, Kyung Ho Lee, S ang Gug Chung, Kyehan Rhee

Abstract:

Microbubbbles incorporating ultrasound have been used to increase the efficacy of targeted drug delivery, because microstreaming induced by cavitating bubbles affects the drug perfusion into the target cells and tissues. In order to clarify the physical effects of microstreaming on drug perfusion into tissues, a preliminary experimental study of perfusion enhancement by a stably oscillating microbubble was performed. Microstreaming was induced by an oscillating bubble at 15 kHz, and perfusion of dye into an agar phantom was optically measured by histology on agar phantom. Surface color intensity and the penetration length of dye in the agar phantom were increased more than 70% and 30%, respectively, due to the microstreaming induced by an oscillating bubble. The mass of dye perfused into a tissue phantom for 30 s was increased about 80% in the phantom with an oscillating bubble. This preliminary experiment shows the physical effects of steady streaming by an oscillating bubble can enhance the drug perfusion into the tissues while minimizing the biological effects.

Keywords: Bubble, Mass Transfer, Microstreaming, Drug Delivery, Acoustic Wave.

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Authors: A. Movaghar, R. A. Mahdavinejad

Abstract:

To analyze the effect of various parameters of fluid on the material properties such as surface and depth defects and/or cracks, it is possible to determine the affection of pressure field on these specifications. Stress tensor analysis is also able to determine the points in which the probability of defection creation is more. Besides, from pressure field, it is possible to analyze the affection of various fluid specifications such as viscosity and density on defect created in the material. In this research, the concerned boundary conditions are analyzed first. Then the solution network and stencil used are mentioned. With the determination of relevant equation on the fluid flow between notch and matrix and their discretion according to the governed boundary conditions, these equations can be solved. Finally, with the variation creations on fluid parameters such as density and viscosity, the affection of these variations can be determined on pressure field. In this direction, the flowchart and solution algorithm with their results as vortex and current function contours for two conditions with most applications in pressing process are introduced and discussed.

Keywords: Pressing, notch, matrix, flow function, vortex.

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Authors: Gül Çevik

Abstract:

This paper summarizes the work conducted to assess the root cause of the failure of a medium commercial vehicle leaf spring failed in service. Macro- and micro-fractographic analyses by scanning electron microscope as well as material verification tests were conducted in order to understand the failure mechanisms and root cause of the failure. Findings from the fractographic analyses indicated that failure mechanism is fatigue. Crack initiation was identified to have occurred from a point on the top surface near to the front face and to the left side. Two other crack initiation points were also observed, however, these cracks did not propagate. The propagation mode of the fatigue crack revealed that the cyclic loads resulting in crack initiation and propagation were unidirectional bending. Fractographic analyses have also showed that the root cause of the fatigue crack initiation and propagation was loading the part above design stress. Material properties of the part were also verified by chemical composition analysis, microstructural analysis by optical microscopy and hardness tests.

Keywords: Leaf spring, failure analysis, fatigue, fractography.

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Authors: Behzad Mohammadzadeh, Huyk Chun Noh

Abstract:

Plate is one of the popular structural elements used in a wide range of industries and structures. They may be subjected to blast loads during explosion events, missile attacks or aircraft attacks. This study is to investigate dynamic responses of the rectangular plate subjected to explosive loads. The effects of material properties and plate thickness on responses of the plate are to be investigated. The compressive pressure is applied to the surface of the plate. Different amounts of thickness in the range from 1mm to 30mm are considered for the plate to evaluate the changes in responses of the plate with respect to plate thickness. Two different properties are considered for the steel. First, the analysis is performed by considering only the elastic-plastic properties for the steel plate. Later on damping is considered to investigate its effects on the responses of the plate. To do analysis, numerical method using a finite element based package ABAQUS is applied. Finally, dynamic responses and graphs showing the relation between maximum displacement of the plate and aim parameters are provided.

Keywords: Impulsive loaded plates, dynamic analysis, abaqus, material nonlinearity.

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Authors: John Abraham, Ramesh Saravana Kumar, Francis Xavier, Deepak Mathew

Abstract:

Broiler slaughter waste has become a major source of pollution throughout the world. Utilization of broiler slaughter waste by dry rendering process produced Rendered Chicken Oil (RCO), a cheap raw material for biodiesel production and Carcass Meal a feed ingredient for pets and fishes. Conversion of RCO into biodiesel may open new vistas for generating wealth from waste besides controlling the major havoc of environmental pollution. A two-step process to convert RCO to good quality Biodiesel was invented. Acid catalysed esterification of FFA followed by base catalysed transesterification of triglycerides was carried out after meticulously standardizing the methanol molar ratio, catalyst concentration, reaction temperature, and reaction time to obtain the maximum biodiesel yield of 97.62% and lowest glycerol yield of 6.96%. RCO biodiesel blend was tested in a CRDI diesel engine. The results revealed that the blending of commercial diesel with 20% RCO biodiesel (B20) lead to less engine wear, a quieter engine and better fuel economy. The better lubricating qualities of RCO B20 prevented over heating of engine, which prolongs the engine life. RCO B20 can reduce the import of crude oil and substantially reduce the engine emissions as proved by significantly lower smoke levels, thus mitigating climatic changes.

Keywords: Biodiesel, Broiler Waste, Engine Testing, Rendered Chicken Oil.

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Authors: F.Ebrahimi, A.Rastgo

Abstract:

Analytical investigation of the free vibration behavior of circular functionally graded (FG) plates integrated with two uniformly distributed actuator layers made of piezoelectric (PZT4) material on the top and bottom surfaces of the circular FG plate based on the classical plate theory (CPT) is presented in this paper. The material properties of the functionally graded substrate plate are assumed to be graded in the thickness direction according to the power-law distribution in terms of the volume fractions of the constituents and the distribution of electric potential field along the thickness direction of piezoelectric layers is simulated by a quadratic function. The differential equations of motion are solved analytically for clamped edge boundary condition of the plate. The detailed mathematical derivations are presented and Numerical investigations are performed for FG plates with two surface-bonded piezoelectric layers. Emphasis is placed on investigating the effect of varying the gradient index of FG plate on the free vibration characteristics of the structure. The results are verified by those obtained from threedimensional finite element analyses.

Keywords: Circular plate, CPT, Functionally graded, Piezoelectric.

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Authors: D. Erdenechimeg, T. Bujinlkham, N. Erdenepurev

Abstract:

The anti-corrosion performance of fatty acid coated mild steel samples is studied. Samples of structural steel coated with collector reagents deposited from surfactant in ethanol solution and overcoated with an epoxy barrier paint. A quantitative corrosion rate was determined by linear polarization resistance method using biopotentiostat/galvanostat 400. Coating morphology was determined by scanning electronic microscopy. A test for hydrophobic surface of steel by surfactant was done. From the samples, the main component or high content iron was determined by chemical method and other metal contents were determined by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES) method. Prior to measuring the corrosion rate, mechanical and chemical treatments were performed to prepare the test specimens. Overcoating the metal samples with epoxy barrier paint after exposing them with surfactant the corrosion rate can be inhibited by 34-35 µm/year.

Keywords: Corrosion, linear polarization resistance, coating, surfactant.

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Authors: Simona Dostalova, Ana Maria Jimenez Jimenez, Marketa Vaculovicova, Vojtech Adam, Rene Kizek

Abstract:

Various nanomaterials can be used as a drug delivery vehicles in nanomedicine, called nanocarriers. They can either be organic or inorganic, synthetic or natural-based. Although synthetic nanocarriers are easier to produce, they can often be toxic for the organism and thus not suitable for use in treatment. From naturalbased nanocarriers, the most commonly used are protein cages or viral capsids. In this work, virus bacteriophage λ was used for delivery of different cytotoxic drugs (cisplatin, carboplatin, oxaliplatin and doxorubicin). Large quantities of phage λ were obtained from phage λ-producing strain of E. coli cultivated in medium with 0.2% maltose. After killing of E. coli with chloroform and its removal by centrifugation, the phage was concentrated by ultracentrifugation at 130 000×g and 4°C for 3 h. The encapsulation of the drugs was performed by infusion method and four different concentrations of the drugs were encapsulated (200; 100; 50; 25 μg·mL-1). Free drug molecules were removed by filtration. The encapsulation was verified using the absorbance for doxorubicin and atomic absorption spectrometry for platinum cytostatics. The amount of encapsulated drug linearly increased with the increasing concentration of applied drug with the determination coefficient R2=0.989 for doxorubicin; R2=0.967 for cisplatin; R2=0.989 for carboplatin and R2=0.996 for oxaliplatin. The overall encapsulation efficiency was calculated as 50% for doxorubicin; 8% for cisplatin; 6% for carboplatin and 10% for oxaliplatin.

Keywords: Bacteriophage λ, doxorubicin, platinum cytostatics, protein-based nanocarrier, viral capsid.

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Authors: Emma R. Arakelova, Stepan G. Grigoryan, Ashot M. Khachatryan, Karapet E. Avjyan, Lilia M. Savchenko, Flora G. Arsenyan

Abstract:

The paper presents a new drugs delivery system, based on the thin film technology. As a model antitumor drug, highly toxic doxorubicin is chosen. The system is based on the technology of obtaining zinc oxide composite of doxorubicin by deposition of nanosize ZnO films on the surface of doxorubicin coating on glass substrate using DC magnetron sputtering of zinc targets in Ar:O₂ medium at room temperature. For doxorubicin zinc oxide compositions in the form of coatings and gels with 180-200nm thick ZnO films, higher (by a factor 2) in vivo (ascitic Ehrlich's carcinoma) antitumor activity is observed at low doses of doxorubicin in comparison with that of the initial preparation at therapeutic doses. The vector character of the doxorubicin zinc oxide composite transport to tumor tissues ensures the increase in antitumor activity as well as decrease of toxicity in comparison with the initial drug.

Keywords: Antitumor activity, doxorubicin, DC magnetron sputtering, zinc oxide.

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Authors: Srinivasa Reddy Mallampati, Chi-Hyeon Lee, Nguyen Thi Thanh Truc, Byeong-Kyu Lee

Abstract:

In the present study, feasibility of the selective surface hydrophilization of polyvinyl chloride (PVC) by microwave treatment was evaluated to facilitate the separation from automotive shredder residue (ASR), by the froth flotation. The combination of 60 sec microwave treatment with PAC, a sharp and significant decrease about 16.5° contact angle of PVC was observed in ASR plastic compared with other plastics. The microwave treatment with the addition of PAC resulted in a synergetic effect for the froth flotation, which may be a result of the 90% selective separation of PVC from ASR plastics, with 82% purity. While, simple mixing with a nanometallic Ca/CaO/PO4 dispersion mixture immobilized 95-100% of heavy metals in ASR soil/residues. The quantity of heavy metals leached from thermal residues after treatment by nanometallic Ca/CaO/PO4 was lower than the Korean standard regulatory limit for hazardous waste landfills. Microwave treatment can be a simple and effective method for PVC separation from ASR plastics.

Keywords: Automotive shredder residue, microwave treatment, chlorinated plastics, separation, heavy metals, Immobilization, separation.

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Authors: Ashutosh Kumar Choudhary, Satish Kumar, Chhaya Sharma

Abstract:

This study evaluates the performance of horizontal subsurface flow constructed wetland (HSSF-CW) for the removal of chlorinated resin and fatty acids (RFAs) from pulp and paper mill wastewater. The dimensions of the treatment system were 3.5 m x 1.5 m x 0.28 m with surface area of 5.25 m2, filled with fine sand and gravel. The cell was planted with an ornamental plant species Canna indica. The removal efficiency of chlorinated RFAs was in the range of 92-96% at the hydraulic retention time (HRT) of 5.9 days. Plant biomass and soil (sand and gravel) were analyzed for chlorinated RFAs content. No chlorinated RFAs were detected in plant biomass but detected in soil samples. Mass balance studies of chlorinated RFAs in HSSF-CW were also carried out.

Keywords: Canna indica, Chlorinated resin & fatty acids, Constructed wetland, Pulp and paper mill wastewater.

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Authors: A. Maria G. Melero, A. M. Senna, J. A. Domingues, M. A. Hausen, E. Aparecida R. Duek, V. R. Botaro

Abstract:

A hydrogel from cellulose acetate cross linked with ethylenediaminetetraacetic dianhydride (HAC-EDTA) was synthesized by our research group, and submitted to characterization and biological tests. Cytocompatibility analysis was performed by confocal microscopy using human adipocyte derived stem cells (ASCs). The FTIR analysis showed characteristic bands of cellulose acetate and hydroxyl groups and the tensile tests evidence that HAC-EDTA present a Young’s modulus of 643.7 MPa. The confocal analysis revealed that there was cell growth at the surface of HAC-EDTA. After one day of culture the cells presented spherical morphology, which may be caused by stress of the sequestration of Ca²⁺ and Mg²⁺ ions at the cell medium by HAC-EDTA, as demonstrated by ICP-MS. However, after seven days and 14 days of culture, the cells present fibroblastoid morphology, phenotype expected by this cellular type. The results give efforts to indicate this new material as a potential biomaterial for tissue engineering, in the future in vivo approach.

Keywords: Cellulose acetate, hydrogel, biomaterial, cellular growth.

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Authors: P.P. Diale, S.S.L. Mkhize, E. Muzenda, J. Zimba

Abstract:

For more than 120 years, gold mining formed the backbone the South Africa-s economy. The consequence of mine closure was observed in large-scale land degradation and widespread pollution of surface water and groundwater. This paper investigates the feasibility of using natural zeolite in removing heavy metals contaminating the Wonderfonteinspruit Catchment Area (WCA), a water stream with high levels of heavy metals and radionuclide pollution. Batch experiments were conducted to study the adsorption behavior of natural zeolite with respect to Fe2+, Mn2+, Ni2+, and Zn2+. The data was analysed using the Langmuir and Freudlich isotherms. Langmuir was found to correlate the adsorption of Fe2+, Mn2+, Ni2+, and Zn2+ better, with the adsorption capacity of 11.9 mg/g, 1.2 mg/g, 1.3 mg/g, and 14.7 mg/g, respectively. Two kinetic models namely, pseudo-first order and pseudo second order were also tested to fit the data. Pseudo-second order equation was found to be the best fit for the adsorption of heavy metals by natural zeolite. Zeolite functionalization with humic acid increased its uptake ability.

Keywords: gold-mining, natural zeolites, water pollution, WestRand.

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Authors: T. Shyam Ramanath, A. Sudharsan, A. Kavitha

Abstract:

As is needless to say; a majority of accidents, which occur, are due to drunk driving. As such, there is no effective mechanism to prevent this. Here we have designed an integrated system for the same purpose. Alcohol content in the driver-s body is detected by means of an infrared breath analyzer placed at the steering wheel. An infrared cell directs infrared energy through the sample and any unabsorbed energy at the other side is detected. The higher the concentration of ethanol, the more infrared absorption occurs (in much the same way that a sunglass lens absorbs visible light, alcohol absorbs infrared light). Thus the alcohol level of the driver is continuously monitored and calibrated on a scale. When it exceeds a particular limit the fuel supply is cutoff. If the device is removed also, the fuel supply will be automatically cut off or an alarm is sounded depending upon the requirement. This does not happen abruptly and special indicators are fixed at the back to avoid inconvenience to other drivers using the highway signals. Frame work for integration of sensors and control module in a scalable multi-agent system is provided .A SMS which contains the current GPS location of the vehicle is sent via a GSM module to the police control room to alert the police. The system is foolproof and the driver cannot tamper with it easily. Thus it provides an effective and cost effective solution for the problem of drunk driving in vehicles.

Keywords: Global system monitoring, global positioning system.

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Authors: M. Baryshev, D.Vainauska, S. Kozireva, A.Karpovs

Abstract:

Liposomal magnetofection is the most powerful nonviral method for the nucleic acid delivery into the cultured cancer cells and widely used for in vitro applications. Use of the static magnetic field condition may result in non-uniform distribution of aggregate complexes on the surface of cultured cells. To prevent this, we developed the new device which allows to concentrate aggregate complexes under dynamic magnetic field, assisting more contact of these complexes with cellular membrane and, possibly, stimulating endocytosis. Newly developed device for magnetofection under dynamic gradient magnetic field, “DynaFECTOR", was used to compare transfection efficiency of human liver hepatocellular carcinoma cell line HepG2 with that obtained by lipofection and magnetofection. The effect of two parameters on transfection efficiency, incubation time under dynamic magnetic field and rotation frequency of magnet, was estimated. Liposomal magnetofection under dynamic gradient magnetic field showed the highest transfection efficiency for HepG2 cells.

Keywords: Dynamic magnetic field, Lipofection, Magnetofection

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Authors: Moaz H. Ali, M. N. M. Ansari

Abstract:

This paper presents a study the effect of nose radius (Rz-mm) on cutting force components and temperatures during the machining simulation in an orthogonal cutting process for titanium alloy (Ti-6Al-4V). The cutting process was performed at various nose radiuses (Rz-mm) while the depth of cut (d-mm), feed rate (fmm/ tooth) and cutting speed (vc-m/ min) were remained constant. The main cutting force (Fc), feed cutting force (Ft) and temperatures were estimated by using finite element modeling (FEM) through ABAQUS/EXPLICIT software and the simulation was developed the two-dimension via an orthogonal cutting process during machining titanium alloy (Ti-6Al-4V). The results led to the conclusion that the nose radius (Rz-mm) has affected directly on the cutting force components. However, temperature gave no indication or has no significant relation with nose radius during machining titanium alloy (Ti-6Al-4V). Hence, any increase or decrease in the nose radius (Rzmm) during machining operation led to effect on the cutting forces and thus it will be effective on surface finish, quality, and quantity of products.

Keywords: Finite element modeling (FEM), nose radius, cutting force, temperature, titanium alloy (Ti-6Al-4V).

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Authors: Tian Kai, Yang Sheng-sheng, Li De-tian, Miao Yu-jun, Xue Yu-xiong Wang Yi, Yan Ze-dong, Ma Ya-li, ZHuang Jian-hong

Abstract:

The charge-exchange xenon (CEX) ion generated by ion thruster can backflow to the surface of spacecraft and threaten to the safety of spacecraft operation. In order to evaluate the effects of the induced plasma environment in backflow regions on the spacecraft, we designed a spherical single Langmuir probe of 5.8cm in diameter for measuring low-density plasma parameters in backflow region of ion thruster. In practice, the tests are performed in a two-dimensional array (40cm×60cm) composed of 20 sites. The experiment results illustrate that the electron temperature ranges from 3.71eV to 3.96eV, with the mean value of 3.82eV and the standard deviation of 0.064eV. The electron density ranges from 8.30×1012/m3 to 1.66×1013/m3, with the mean value of 1.30×1013/m3 and the standard deviation of 2.15×1012/m3. All data is analyzed according to the “ideal" plasma conditions of Maxwellian distributions.

Keywords: Langmuir Probe, Plasma parameters, Ion thruster, Backflow region.

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Authors: F. Elashhab, L. Sheha, R. Fawzi Elsupikhe, A. E. A. Youssef, R. M. Sheltami, T. Alfazani

Abstract:

Chitosans, CSs, in solution are increasingly used in a range of geometric properties in various academic and industrial sectors, especially in the domain of pharmaceutical and biomedical engineering. In order to provide a tailoring guide of CSs to the applicants, gamma (γ)-irradiation technology and simple viscosity measurements have been used in this study. Accordingly, CS solid discs (0.5 cm thickness and 2.5 cm diameter) were exposed in air to Cobalt-60 (γ)-radiation, at room temperature and constant 50 kGy dose for different periods of exposer time (tγ). Diluted solutions of native and different irradiated CS were then prepared by dissolving 1.25 mg cm-3 of each polymer in 0.1 M NaCl/0.2 M CH3COOH. The single-concentration relative viscosity (ƞr) measurements were employed to obtain their intrinsic viscosity ([ƞ]) values and interrelated parameters, like: the molar mass (Mƞ), hydrodynamic radiuses (RH,ƞ), radius of gyration (RG,ƞ), and second virial coefficient (A2,ƞ) of CSs in the solution. The results show an exponential decrease of ƞr, [ƞ], Mƞ, RH,ƞ and RG,ƞ with increasing tγ. This suggests the influence of random chain-scission of CSs glycosidic bonds, with rate constant kr and kr-1 (lifetime τr ~ 0.017 min-1 and 57.14 min, respectively). The results also show an exponential decrease of A2ƞ with increasing tγ, which can be attributed to the growth of excluded volume effect in CS segments by tγ and, hence, better solution quality. The results are represented in following scaling laws as a tailoring guide to the applicants: RH,ƞ = 6.98 x 10-3 Mr0.65; RG,ƞ = 7.09 x 10-4 Mr0.83; A2,ƞ = 121.03 Mƞ,r-0.19.

Keywords: Gamma irradiation, geometric properties, kinetic model, scaling laws, viscosity measurement.

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Authors: Mohamed Amine Belmiloud, Nord Eddine Sad Chemloul

Abstract:

In this study, we investigated numerically heat transfer by mixed convection coupled to radiation in a square cavity; the upper horizontal wall is movable. The purpose of this study is to see the influence of the emissivity ε and the varying of the Richardson number Ri on the variation of average Nusselt number Nu. The vertical walls of the cavity are differentially heated, the left wall is maintained at a uniform temperature higher than the right wall, and the two horizontal walls are adiabatic. The finite volume method is used for solving the dimensionless Governing Equations. Emissivity values used in this study are ranged between 0 and 1, the Richardson number in the range 0.1 to 10. The Rayleigh number is fixed to Ra=104 and the Prandtl number is maintained constant Pr=0.71. Streamlines, isothermal lines and the average Nusselt number are presented according to the surface emissivity. The results of this study show that the Richardson number Ri and emissivity ε affect the average Nusselt number.

Keywords: Numerical study, mixed convection, square cavity, wall emissivity, lid-driven.

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Authors: Javier Sedano-Cibrián, Julio Manuel de Luis-Ruiz, Rubén Pérez-Álvarez, Raúl Pereda-García, Beatriz Malagón-Picón

Abstract:

Structural inspection activities are necessary to ensure the correct functioning of infrastructures. UAV techniques have become more popular than traditional techniques. Specifically, UAV Photogrammetry allows time and cost savings. The development of this technology has permitted the use of low-cost thermal sensors in UAVs. The representation of 3D thermal models with this type of equipment is in continuous evolution. The direct processing of thermal images usually leads to errors and inaccurate results. In this paper, a methodology is proposed for the generation of 3D thermal models using dual sensors, which involves the application of RGB and thermal images in parallel. Hence, the RGB images are used as the basis for the generation of the model geometry, and the thermal images are the source of the surface temperature information that is projected onto the model. Mining/industrial facilities representations that are obtained can be used for inspection activities.

Keywords: Aerial thermography, data processing, drone, low-cost, point cloud.

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Authors: Hamoud Al-Hadrami, Hossein Emadi, Athar Hussain

Abstract:

Green hydrogen is quickly emerging as a new source of the renewable energy for the world. Hydrogen production using water electrolysis is deemed as an environmentally friendly and safe source of energy for transportation and other industries. However, storing high volumes of hydrogen seems to be a significant challenge. Abandoned hydrocarbon reservoirs are considered as viable hydrogen storage options because of the availability of the required infrastructure such as wells and surface facilities. However, long-term wellbore integrity in these wells could be a serious challenge. The aim of this research is to investigate the effect of stored hydrogen on the wellbore integrity such as casing cement. The methodology is to experimentally expose hydrogen to wet and dry cement and measure the impact on cement rheological and mechanical properties. Hydrogen reduces the compressive strength of a set cement if it gets in contact with the cement slurry. Also, mixing hydrogen with cement slurry slightly increases its density and rheological properties which need to be considered to have a successful primary cementing operation.

Keywords: Green hydrogen, underground storage, wellbore integrity, cement, compressive strength.

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Authors: Gabriela R. Fernandes, Danilo H. Konda, Luiz C. F. Sanches

Abstract:

In this work, the plate bending formulation of the boundary element method - BEM, based on the Reissner?s hypothesis, is extended to the analysis of plates reinforced by beams taking into account the membrane effects. The formulation is derived by assuming a zoned body where each sub-region defines a beam or a slab and all of them are represented by a chosen reference surface. Equilibrium and compatibility conditions are automatically imposed by the integral equations, which treat this composed structure as a single body. In order to reduce the number of degrees of freedom, the problem values defined on the interfaces are written in terms of their values on the beam axis. Initially are derived separated equations for the bending and stretching problems, but in the final system of equations the two problems are coupled and can not be treated separately. Finally are presented some numerical examples whose analytical results are known to show the accuracy of the proposed model.

Keywords: Boundary elements, Building floor structures, Platebending.

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Authors: M. Gomaa, A. J. Alimin, K. A. Kamarudin

Abstract:

Nowadays, the focus on renewable energy and alternative fuels has increased due to increasing oil prices, environment pollution, and also concern on preserving the nature. Biodiesel has been known as an attractive alternative fuel although biodiesel produced from edible oil is very expensive than conventional diesel. Therefore, the uses of biodiesel produced from non-edible oils are much better option. Currently Jatropha biodiesel (JBD) is receiving attention as an alternative fuel for diesel engine. Biodiesel is non-toxic, biodegradable, high lubricant ability, highly renewable, and its use therefore produces real reduction in petroleum consumption and carbon dioxide (CO2) emissions. Although biodiesel has many advantages, but it still has several properties need to improve, such as lower calorific value, lower effective engine power, higher emission of nitrogen oxides (NOX) and greater sensitivity to low temperature. Exhaust gas recirculation (EGR) is effective technique to reduce NOX emission from diesel engines because it enables lower flame temperature and oxygen concentration in the combustion chamber. Some studies succeeded to reduce the NOX emission from biodiesel by EGR but they observed increasing soot emission. The aim of this study was to investigate the engine performance and soot emission by using blended Jatropha biodiesel with different EGR rates. A CI engine that is water-cooled, turbocharged, using indirect injection system was used for the investigation. Soot emission, NOX, CO2, carbon monoxide (CO) were recorded and various engine performance parameters were also evaluated.

Keywords: EGR, Jatropha biodiesel, NOX, Soot emission.

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Authors: A. I. Alateyah, H. N. Dhakal, Z. Y. Zhang

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Contact angle measurement was utilized in order to study the subject of the wettability and surface chemistry of the nanocomposites materials. Water and glycerol droplets were used in this study. The incorporation of layered silicate into the vinyl ester matrix helped to improve the wettability and reduced the θ values of both liquids used. The addition of 2 wt.% clay loading reduced the θ values of water and glycerol by up to 21% and 6% respectively. Likewise, the incorporation of 4 wt.% clay loading reduced the water and glycerol θ values by 49% and 38% respectively. Also this study confirms the findings in the literature regarding the relationship between the intercalation nanocomposites level and the wettability. Wide Angle X-ray Diffraction, Scanning Electron Microscopy and Transmission Electron Microscopy were utilised in order to characterise the interlamellar structure of nanocomposites.

Keywords: Vinyl ester, nanocomposites, layered silicate, characterisations, contact angle measurement, wettability.

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Authors: H. Madi, L. Mouzai, M. Bouhadef

Abstract:

The purpose of this article is to study the effects of plants cover on overland flow and, therefore, its influences on the amount of eroded and transported soil. In this investigation, all the experiments were conducted in the LEGHYD laboratory using a rainfall simulator and a soil tray. The experiments were conducted using an experimental plot (soil tray) which is 2m long, 0.5 m wide and 0.15 m deep. The soil used is an agricultural sandy soil (62,08% coarse sand, 19,14% fine sand, 11,57% silt and 7,21% clay). Plastic rods (4 mm in diameter) were used to simulate the plants at different densities: 0 stem/m2 (bared soil), 126 stems/m², 203 stems/m², 461 stems/m² and 2500 stems/m²). The used rainfall intensity is 73mm/h and the soil tray slope is fixed to 3°. The results have shown that the overland flow velocities decreased with increasing stems density, and the density cover has a great effect on sediment concentration. Darcy–Weisbach and Manning friction coefficients of overland flow increased when the stems density increased. Froude and Reynolds numbers decreased with increasing stems density and, consequently, the flow regime of all treatments was laminar and subcritical. From these findings, we conclude that increasing the plants cover can efficiently reduce soil loss and avoid denuding the roots plants.

Keywords: Soil erosion, vegetation, stems density, overland flow.

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Authors: Z. Rihova, K. Saksl, C. Siemers, D. Ostroushko

Abstract:

Titanium alloys like the modern alloy Ti 6Al 2Sn 4Zr 6Mo (Ti-6246) combine excellent specific mechanical properties and corrosion resistance. On the other hand,due to their material characteristics, machining of these alloys is difficult to perform. The aim of the current study is the analyses of wear mechanisms of coated cemented carbide tools applied in orthogonal cutting experiments of Ti-6246 alloy. Round bars were machined with standard coated tools in dry conditions on a CNC latheusing a wide range of cutting speeds and cutting depths. Tool wear mechanisms were afterwards investigated by means of stereo microscopy, optical microscopy, confocal microscopy and scanning electron microscopy. Wear mechanisms included fracture of the tool tip (total failure) and abrasion. Specific wear features like crater wear, micro cracks and built-up edgeformation appeared depending of the mechanical and thermal conditions generated in the workpiece surface by the cutting action.

Keywords: Alloy 6246, machining, tool wear, optical microscopy, SEM, EDX analysis

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Authors: Tanwarat Kajsongkram, Saowalux Rotamporn, Sirinat Limbunruang, Sirinan Thubthimthed

Abstract:

High Performance Liquid Chromatography (HPLC) method was developed and validated for simultaneous estimation of 6-Gingerol(6G) and 6-Shogaol(6S) in joint pain relief gel containing ginger extract. The chromatographic separation was achieved by using C18 column, 150 x 4.6mm i.d., 5μ Luna, mobile phase containing acetonitrile and water (gradient elution). The flow rate was 1.0 ml/min and the absorbance was monitored at 282 nm. The proposed method was validated in terms of the analytical parameters such as specificity, accuracy, precision, linearity, range, limit of detection (LOD), limit of quantification (LOQ), and determined based on the International Conference on Harmonization (ICH) guidelines. The linearity ranges of 6G and 6S were obtained over 20- 60 and 6-18 μg/ml respectively. Good linearity was observed over the above-mentioned range with linear regression equation Y= 11016x- 23778 for 6G and Y = 19276x-19604 for 6S (x is concentration of analytes in μg/ml and Y is peak area). The value of correlation coefficient was found to be 0.9994 for both markers. The limit of detection (LOD) and limit of quantification (LOQ) for 6G were 0.8567 and 2.8555 μg/ml and for 6S were 0.3672 and 1.2238 μg/ml respectively. The recovery range for 6G and 6S were found to be 91.57 to 102.36 % and 84.73 to 92.85 % for all three spiked levels. The RSD values from repeated extractions for 6G and 6S were 3.43 and 3.09% respectively. The validation of developed method on precision, accuracy, specificity, linearity, and range were also performed with well-accepted results.

Keywords: Ginger, 6-gingerol, HPLC, 6-shogaol.

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Authors: Ali Reza Tahavvor, Saeed Hosseini, Afshin Karimzadeh Fard

Abstract:

In this paper the effect of wall waviness of side walls in a two-dimensional wavy enclosure is numerically investigated. Two vertical wavy walls and straight top wall are kept isothermal and the bottom wall temperature is higher and spatially varying with cosinusoidal temperature distribution. A computational code based on Finite-volume approach is used to solve governing equations and SIMPLE method is used for pressure velocity coupling. Test is performed for several different numbers of undulations. The Prandtl number was kept constant and the Ra number denotes that the flow is laminar. Temperature and velocity fields are determined. Therefore, according to the obtained results a correlation is proposed for average Nusselt number as a function of number of side wall waves. The results indicate that the Nusselt number is highly affected by number of waves and increasing it decreases the wavy walls Nusselt number; although the Nusselt number is not highly affected by surface waviness when the number of undulations is below one.

Keywords: Cavity, natural convection, Nusselt number, wavy wall.

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Authors: L. Haddad, Z. Aouachria

Abstract:

The development of transport systems has negative impacts on the environment although it has beneficial effects on society. The car policy caused many problems such as: - the spectacular growth of fuel consumption hence the very vast increase in urban pollution, traffic congestion in certain places and at certain times, the increase in the number of accidents. The exhaust emissions from cars and weather conditions are the main factors that determine the level of pollution in urban atmosphere. These conditions lead to the phenomenon of heat transfer and radiation occurring between the air and the soil surface of any town. These exchanges give rise, in urban areas, to the effects of heat islands that correspond to the appearance of excess air temperature between the city and its surrounding space. In this object, we perform a numerical simulation of the plume generated by the cars exhaust gases and show that these gases form a screening effect above the urban city which cause the heat island in the presence of wind flow. This study allows us: 1. To understand the different mechanisms of interactions between these phenomena.2. To consider appropriate technical solutions to mitigate the effects of the heat island.

Keywords: Atmospheric pollution, impact on the health, urban transport, heat island.

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