Search results for: output of the main agricultural commodity
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 4672

Search results for: output of the main agricultural commodity

2572 On Reversal and Transposition Medians

Authors: Martin Bader

Abstract:

During the last years, the genomes of more and more species have been sequenced, providing data for phylogenetic recon- struction based on genome rearrangement measures. A main task in all phylogenetic reconstruction algorithms is to solve the median of three problem. Although this problem is NP-hard even for the sim- plest distance measures, there are exact algorithms for the breakpoint median and the reversal median that are fast enough for practical use. In this paper, this approach is extended to the transposition median as well as to the weighted reversal and transposition median. Although there is no exact polynomial algorithm known even for the pairwise distances, we will show that it is in most cases possible to solve these problems exactly within reasonable time by using a branch and bound algorithm.

Keywords: Comparative genomics, genome rearrangements, me-dian, reversals, transpositions.

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2571 Biosensor Measurement of Urea Coonncentration in Human Blood Serum

Authors: O. L. Kukla, S. V. Marchenko, O. A. Zinchenko, O. S. Pavluchenko, O. M. KKuukla, S. V. Dzyadevych, O. P. Soldatkin

Abstract:

An application of the highly biosensor based on pH-sensitive field immobilized urease for urea analysis was demo The main analytical characteristics of the bios determined; the conditions of urea measureme blood were optimized. A conceptual possibility biosensor for detection of urea concentratio patients suffering from renal insufficiency was sensitive and selective effect transistor and monstrated in this work. iosensor developed were ment in real samples of ility of application of the tion in blood serum of as shown.

Keywords: Biosensor, blood serum, pH transistor, urea, urease, field-effect

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2570 Frequency Domain Analysis for Hopf Bifurcation in a Delayed Competitive Web-site Model

Authors: Changjin Xu, Yusen Wu

Abstract:

In this paper, applying frequency domain approach, a delayed competitive web-site system is investigated. By choosing the parameter α as a bifurcation parameter, it is found that Hopf bifurcation occurs as the bifurcation parameter α passes a critical values. That is, a family of periodic solutions bifurcate from the equilibrium when the bifurcation parameter exceeds a critical value. Some numerical simulations are included to justify the theoretical analysis results. Finally, main conclusions are given.

Keywords: Web-site system, stability, Nyquist criterion, Hopf bifurcation, frequency domain.

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2569 Distributional Impacts of Changes in Value Added Tax Rates in the Czech Republic

Authors: Ondřej Bayer

Abstract:

The paper evaluates the ongoing reform of VAT in the Czech Republic in terms of impacts on individual households. The main objective is to analyse the impact of given changes on individual households. The adopted method is based on the data related to household consumption by individual household quintiles; obtained data are subjected to micro-simulation examining. Results are discussed in terms of vertical tax justice. Results of the analysis reveal that VAT behaves regressively and a sole consolidation of rates at a higher level only increases the regression of this tax in the Czech Republic.

Keywords: Consolidation of rates, household quintiles, tax impact, VAT.

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2568 Optimum Cascaded Design for Speech Enhancement Using Kalman Filter

Authors: T. Kishore Kumar

Abstract:

Speech enhancement is the process of eliminating noise and increasing the quality of a speech signal, which is contaminated with other kinds of distortions. This paper is on developing an optimum cascaded system for speech enhancement. This aim is attained without diminishing any relevant speech information and without much computational and time complexity. LMS algorithm, Spectral Subtraction and Kalman filter have been deployed as the main de-noising algorithms in this work. Since these algorithms suffer from respective shortcomings, this work has been undertaken to design cascaded systems in different combinations and the evaluation of such cascades by qualitative (listening) and quantitative (SNR) tests.

Keywords: LMS, Kalman filter, Speech Enhancement and Spectral Subtraction.

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2567 Assessment the Quality of Telecommunication Services by Fuzzy Inferences System

Authors: Oktay Nusratov, Ramin Rzaev, Aydin Goyushov

Abstract:

Fuzzy inference method based approach to the forming of modular intellectual system of assessment the quality of communication services is proposed. Developed under this approach the basic fuzzy estimation model takes into account the recommendations of the International Telecommunication Union in respect of the operation of packet switching networks based on IPprotocol. To implement the main features and functions of the fuzzy control system of quality telecommunication services it is used multilayer feedforward neural network.

Keywords: Quality of communication, IP-telephony, Fuzzy set, Fuzzy implication, Neural network.

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2566 Extended Minimal Controller Synthesis for Voltage-Fed Induction Motor Based on the Hyperstability Theory

Authors: A. Ramdane, F.Naceri, S. Ramdane

Abstract:

in this work, we present a new strategy of direct adaptive control denoted: Extended minimal controller synthesis (EMCS). This algorithm is designed for an induction motor, which includes both electrical and mechanical dynamics under the assumptions of linear magnetic circuits. The main motivation of the EMCS control is to enhance the robustness of the MRAC algorithms, i.e. the rejection of bounded effects of rapidly varying external disturbances.

Keywords: Adaptive Control, Simple model reference adaptive control (SMRAC), Extended Minimal Controller synthesis (EMCS), Induction Motor (IM)

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2565 Cogeneration Unit for Small Stove

Authors: Michal Spilacek, Marian Brazdil, Otakar Stelcl, Jiri Pospisil

Abstract:

This paper shows an experimental testing of a small unit for combustion of solid fuels, such as charcoal and wood logs, that can provide electricity. One of the concepts is that the unit does not require qualified personnel for its operation. The unit itself is composed of two main parts. The design requires a heat producing stove and electricity producing thermoelectric generator. After the construction the unit was tested and the results show that the emission release is within the legislative requirements for emission production and environmental protection. That qualifies such unit for indoor application.

Keywords: Micro-cogeneration, thermoelectric generator, biomass combustion, wood stove.

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2564 What Are the Factors Underlying the Differences between Young Saudi Women in Traditional Families That Choose to Conform to the Society Norms, and Young Saudi Women Who Do Not Conform?

Authors: Mai Al-Subaie

Abstract:

This research suggests that women in traditional families of Saudi Arabia are divided into two groups, the one who conforms to the society and the new type of women that has been emerged due to the changing and development of the culture, who do not want to conform to the rules. The factors underlying the differences were explored by using a test and an interview. And that concluded some of the main factors that were a real affect of why some women still want to follow the society and traditional rules, and other want to break free.

Keywords: Conformity, Non-Conformity, Saudi Arabia, Women.

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2563 Development of a Kinetic Model for the Photodegradation of 4-Chlorophenol using a XeBr Excilamp

Authors: M. Gomez, M. D. Murcia, E. Gomez, J. L. Gomez, N. Christofi

Abstract:

Excilamps are new UV sources with great potential for application in wastewater treatment. In the present work, a XeBr excilamp emitting radiation at 283 nm has been used for the photodegradation of 4-chlorophenol within a range of concentrations from 50 to 500 mg L-1. Total removal of 4-chlorophenol was achieved for all concentrations assayed. The two main photoproduct intermediates formed along the photodegradation process, benzoquinone and hydroquinone, although not being completely removed, remain at very low residual concentrations. Such concentrations are insignificant compared to the 4-chlorophenol initial ones and non-toxic. In order to simulate the process and scaleup, a kinetic model has been developed and validated from the experimental data.

Keywords: 4-chlorophenol, excilamps, kinetic model, photodegradation.

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2562 Oscillation Theorems for Second-order Nonlinear Neutral Dynamic Equations with Variable Delays and Damping

Authors: Da-Xue Chen, Guang-Hui Liu

Abstract:

In this paper, we study the oscillation of a class of second-order nonlinear neutral damped variable delay dynamic equations on time scales. By using a generalized Riccati transformation technique, we obtain some sufficient conditions for the oscillation of the equations. The results of this paper improve and extend some known results. We also illustrate our main results with some examples.

Keywords: Oscillation theorem, second-order nonlinear neutral dynamic equation, variable delay, damping, Riccati transformation.

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2561 Life Cycle Assessment of Seawater Desalinization in Western Australia

Authors: Wahidul K. Biswas

Abstract:

Perth will run out of available sustainable natural water resources by 2015 if nothing is done to slow usage rates, according to a Western Australian study [1]. Alternative water technology options need to be considered for the long-term guaranteed supply of water for agricultural, commercial, domestic and industrial purposes. Seawater is an alternative source of water for human consumption, because seawater can be desalinated and supplied in large quantities to a very high quality. While seawater desalination is a promising option, the technology requires a large amount of energy which is typically generated from fossil fuels. The combustion of fossil fuels emits greenhouse gases (GHG) and, is implicated in climate change. In addition to environmental emissions from electricity generation for desalination, greenhouse gases are emitted in the production of chemicals and membranes for water treatment. Since Australia is a signatory to the Kyoto Protocol, it is important to quantify greenhouse gas emissions from desalinated water production. A life cycle assessment (LCA) has been carried out to determine the greenhouse gas emissions from the production of 1 gigalitre (GL) of water from the new plant. In this LCA analysis, a new desalination plant that will be installed in Bunbury, Western Australia, and known as Southern Seawater Desalinization Plant (SSDP), was taken as a case study. The system boundary of the LCA mainly consists of three stages: seawater extraction, treatment and delivery. The analysis found that the equivalent of 3,890 tonnes of CO2 could be emitted from the production of 1 GL of desalinated water. This LCA analysis has also identified that the reverse osmosis process would cause the most significant greenhouse emissions as a result of the electricity used if this is generated from fossil fuels

Keywords: Desalinization, Greenhouse gas emissions, life cycle assessment.

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2560 Study on the Design of Supermarket Store Layouts: The Principle of “Sales Magnet“

Authors: Masao Ohta, Yoshiyuki Higuchi

Abstract:

This study analyses store layout among the many factors that underlie supermarket store design, this; in terms of what to display in a shop and where to place the items. This report examines newly-opened stores and evaluates their interior shop floor layouts, which we then attempt to categorize by various styles. We then consider the interaction between shop floor layout and customer behavior from the perspective of the supermarket as the seller. At this point, we focus on the “store magnets"–the main sections within the shop likely to attract customers into the store.

Keywords: Supermarket Store Layout, Sales magnet, Customer Circulation Rate, Section Drop-by Rates.

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2559 Utilizing 5G Mobile Connection as a Node in Layer 1 Proof of Authority Blockchain Used for Microtransaction

Authors: Frode van der Laak

Abstract:

The paper contributes to the feasibility of using a 5G mobile connection as a node for a Proof of Authority (PoA) blockchain, which is used for microtransactions at the same time. It uses the phone number identity of the users that are linked to the crypto wallet address. It also proposed a consensus protocol based on PoA blockchain; PoA is a permission blockchain where consensus is achieved through a set of designated authority rather than through mining, as is the case with a Proof of Work (PoW) blockchain. This report will first explain the concept of a PoA blockchain and how it works. It will then discuss the potential benefits and challenges of using a 5G mobile connection as a node in such a blockchain, and finally, the main open problem statement and proposed solutions with the requirements.

Keywords: 5G, mobile, connection, node, PoA, blockchain, microtransaction.

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2558 Research of the Main Indexes of Freshness Anchovy (Engraulis engrasicolus Linnaeus, 1758) and Sardines (Sardina pilchardus Walbaum 1792) of Mediterranean

Authors: G.R.A. Alberio, D. Scalone, G. Spagna

Abstract:

Anchovy (Engraulis Encrasicholus) and sardine (Sardina Pilchardus) are blue fishes linked to our alimentary tradition of Mediterranean. In our work, particularly, we tested for the first time physical and enzymatic methods to verify the freshness of species of blue fish, anchovy and sardine of Mediterranean. In connection with to the lowering of the pH after post-mortem stage we assisted to a increase in proteolytic activity of calpaine and catpsine. Already after 2 h in post-mortem there was a significant increase.

Keywords: Engraulis encrasicholus, Sardina pilchardus, freshness, index rigor.

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2557 Mathematical Reconstruction of an Object Image Using X-Ray Interferometric Fourier Holography Method

Authors: M. K. Balyan

Abstract:

The main principles of X-ray Fourier interferometric holography method are discussed. The object image is reconstructed by the mathematical method of Fourier transformation. The three methods are presented – method of approximation, iteration method and step by step method. As an example the complex amplitude transmission coefficient reconstruction of a beryllium wire is considered. The results reconstructed by three presented methods are compared. The best results are obtained by means of step by step method.

Keywords: Dynamical diffraction, hologram, object image, X-ray holography.

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2556 The removal of Ni, Cu and Fe from a Mixed Metal System using Sodium Hypophosphite as a Reducing Agent

Authors: Promise Sethembiso Ngema, Freeman Ntuli, Mohamed Belaid

Abstract:

The main objective of this study was to remove and recover Ni, Cu and Fe from a mixed metal system using sodium hypophosphite as a reducing agent and nickel powder as seeding material. The metal systems studied consisted of Ni-Cu, Ni-Fe and Ni-Cu-Fe solutions. A 5 L batch reactor was used to conduct experiments where 100 mg/l of each respective metal was used. It was found that the metals were reduced to their elemental form with removal efficiencies of over 80%. The removal efficiency decreased in the order Fe>Ni>Cu. The metal powder obtained contained between 97-99% Ni and was almost spherical and porous. Size enlargement by aggregation was the dominant particulate process.

Keywords: crystallization, electroless plating, heavy metal removal, wastewater treatment

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2555 Aircraft Gas Turbine Engines Technical Condition Identification System

Authors: A. M. Pashayev, C. Ardil, D. D. Askerov, R. A. Sadiqov, P. S. Abdullayev

Abstract:

In this paper is shown that the probability-statistic methods application, especially at the early stage of the aviation gas turbine engine (GTE) technical condition diagnosing, when the flight information has property of the fuzzy, limitation and uncertainty is unfounded. Hence is considered the efficiency of application of new technology Soft Computing at these diagnosing stages with the using of the Fuzzy Logic and Neural Networks methods. Training with high accuracy of fuzzy multiple linear and non-linear models (fuzzy regression equations) which received on the statistical fuzzy data basis is made. Thus for GTE technical condition more adequate model making are analysed dynamics of skewness and kurtosis coefficients' changes. Researches of skewness and kurtosis coefficients values- changes show that, distributions of GTE work parameters have fuzzy character. Hence consideration of fuzzy skewness and kurtosis coefficients is expedient. Investigation of the basic characteristics changes- dynamics of GTE work parameters allows to draw conclusion on necessity of the Fuzzy Statistical Analysis at preliminary identification of the engines' technical condition. Researches of correlation coefficients values- changes shows also on their fuzzy character. Therefore for models choice the application of the Fuzzy Correlation Analysis results is offered. For checking of models adequacy is considered the Fuzzy Multiple Correlation Coefficient of Fuzzy Multiple Regression. At the information sufficiency is offered to use recurrent algorithm of aviation GTE technical condition identification (Hard Computing technology is used) on measurements of input and output parameters of the multiple linear and non-linear generalised models at presence of noise measured (the new recursive Least Squares Method (LSM)). The developed GTE condition monitoring system provides stage-bystage estimation of engine technical conditions. As application of the given technique the estimation of the new operating aviation engine temperature condition was made.

Keywords: Gas turbine engines, neural networks, fuzzy logic, fuzzy statistics.

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2554 Comparative Analysis of Chemical Composition and Biological Activities of Ajuga genevensis L. in in vitro Culture and Intact Plants

Authors: Naira Sahakyan, Margarit Petrosyan, Armen Trchounian

Abstract:

One of the tasks in contemporary biotechnology, pharmacology and other fields of human activities is to obtain biologically active substances from plants. They are very essential in the treatment of many diseases due to their actually high therapeutic value without visible side effects. However, sometimes the possibility of obtaining the metabolites is limited due to the reduction of wild-growing plants. That is why the plant cell cultures are of great interest as alternative sources of biologically active substances. Besides, during the monitored cultivation, it is possible to obtain substances that are not synthesized by plants in nature. Isolated culture of Ajuga genevensis with high growth activity and ability of regeneration was obtained using MS nutrient medium. The agar-diffusion method showed that aqueous extracts of callus culture revealed high antimicrobial activity towards various gram-positive (Bacillus subtilis A1WT; B. mesentericus WDCM 1873; Staphylococcus aureus WDCM 5233; Staph. citreus WT) and gram-negative (Escherichia coli WKPM M-17; Salmonella typhimurium TA 100) microorganisms. The broth dilution method revealed that the minimal and half maximal inhibitory concentration values against E. coli corresponded to the 70 μg/mL and 140 μg/mL concentration of the extract respectively. According to the photochemiluminescent analysis, callus tissue extracts of leaf and root origin showed higher antioxidant activity than the same quantity of A. genevensis intact plant extract. A. genevensis intact plant and callus culture extracts showed no cytotoxic effect on K-562 suspension cell line of human chronic myeloid leukemia. The GC-MS analysis showed deep differences between the qualitative and quantitative composition of callus culture and intact plant extracts. Hexacosane (11.17%); n-hexadecanoic acid (9.33%); and 2-methoxy-4-vinylphenol (4.28%) were the main components of intact plant extracts. 10-Methylnonadecane (57.0%); methoxyacetic acid, 2-tetradecyl ester (17.75%) and 1-Bromopentadecane (14.55%) were the main components of A. genevensis callus culture extracts. Obtained data indicate that callus culture of A. genevensis can be used as an alternative source of biologically active substances.

Keywords: Ajuga genevensis, antibacterial activity, antioxidant activity, callus cultures.

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2553 A Way of Converting Color Images to Gray Scale Ones for the Color-Blind -Applying to the Part of the Tokyo Subway Map-

Authors: Katsuhiro Narikiyo, Shota Hashikawa

Abstract:

This paper proposes a way of removing noises and reducing the number of colors contained in a JPEG image. Main purpose of this project is to convert color images to monochrome images for the color-blind. We treat the crispy color images like the Tokyo subway map. Each color in the image has an important information. But for the color blinds, similar colors cannot be distinguished. If we can convert those colors to different gray values, they can distinguish them. Therefore we try to convert color images to monochrome images.

Keywords: Color-blind, JPEG, Monochrome image, Denoise.

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2552 Investigation of Relationship between Organizational Climate and Organizational Citizenship Behavior: A Research on Health Sector

Authors: Serdar Öge, Pınar Erdogan

Abstract:

The main objective of this research is to describe the relationship between organizational climate and organizational citizenship behavior. In order to examine this relationship, a research is intended to be carried out in relevant institutions and organizations operating in the health sector in Turkey. It will be researched that whether there is a statistically significant relationship between organizational climate and organizational citizenship behavior through elated scientific research methods and statistical analysis. In addition, relationships between the dimensions of organizational climate and organizational citizenship behavior subscales will be questioned statistically.

Keywords: Organizational climate, organizational citizenship, organizational citizenship behavior, climate.

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2551 Representing Data without Lost Compression Properties in Time Series: A Review

Authors: Nabilah Filzah Mohd Radzuan, Zalinda Othman, Azuraliza Abu Bakar, Abdul Razak Hamdan

Abstract:

Uncertain data is believed to be an important issue in building up a prediction model. The main objective in the time series uncertainty analysis is to formulate uncertain data in order to gain knowledge and fit low dimensional model prior to a prediction task. This paper discusses the performance of a number of techniques in dealing with uncertain data specifically those which solve uncertain data condition by minimizing the loss of compression properties.

Keywords: Compression properties, uncertainty, uncertain time series, mining technique, weather prediction.

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2550 CASTE: a Cloud-Based Automatic Software Test Environment

Authors: Fuyang Peng, Bo Deng, Chao Qi

Abstract:

This paper presents the design and implementation of CASTE, a Cloud-based automatic software test environment. We first present the architecture of CASTE, then the main packages and classes of it are described in detail. CASTE is built upon a private Infrastructure as a Service platform. Through concentrated resource management of virtualized testing environment and automatic execution control of test scripts, we get a better solution to the testing resource utilization and test automation problem. Experiments on CASTE give very appealing results.

Keywords: Software testing, test environment, test script, cloud computing, IaaS, test automation.

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2549 Effect of Halo Protection Device on the Aerodynamic Performance of Formula Racecar

Authors: Mark Lin, Periklis Papadopoulos

Abstract:

This paper explores the aerodynamics of the formula racecar when a ‘halo’ driver-protection device is added to the chassis. The halo protection device was introduced at the start of the 2018 racing season as a safety measure against foreign object impacts that a driver may encounter when driving an open-wheel racecar. In the one-year since its introduction, the device has received wide acclaim for protecting the driver on two separate occasions. The benefit of such a safety device certainly cannot be disputed. However, by adding the halo device to a car, it changes the airflow around the vehicle, and most notably, to the engine air-intake and the rear wing. These negative effects in the air supply to the engine, and equally to the downforce created by the rear wing are studied in this paper using numerical technique, and the resulting CFD outputs are presented and discussed. Comparing racecar design prior to and after the introduction of the halo device, it is shown that the design of the air intake and the rear wing has not followed suit since the addition of the halo device. The reduction of engine intake mass flow due to the halo device is computed and presented for various speeds the car may be going. Because of the location of the halo device in relation to the air intake, airflow is directed away from the engine, making the engine perform less than optimal. The reduction is quantified in this paper to show the correspondence to reduce the engine output when compared to a similar car without the halo device. This paper shows that through aerodynamic arguments, the engine in a halo car will not receive unobstructed, clean airflow that a non-halo car does. Another negative effect is on the downforce created by the rear wing. Because the amount of downforce created by the rear wing is influenced by every component that comes before it, when a halo device is added upstream to the rear wing, airflow is obstructed, and less is available for making downforce. This reduction in downforce is especially dramatic as the speed is increased. This paper presents a graph of downforce over a range of speeds for a car with and without the halo device. Acknowledging that although driver safety is paramount, the negative effect of this safety device on the performance of the car should still be well understood so that any possible redesign to mitigate these negative effects can be taken into account in next year’s rules regulation.

Keywords: Automotive aerodynamics, halo device, downforce. engine intake.

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2548 Mobility Analysis of the Population of Rabat-Salé-Zemmour-Zaer

Authors: F. Ghaiti

Abstract:

In this paper, we present the 2006 survey study origin destination and price that we carried out during 2006 fall in the area in the Moroccan region of Rabat-Salé-Zemmour-Zaer. The survey concerns the people-s characteristics, their displacements behavior and the price that they will be able to pay for a tramway ticket. The main objective is to study a set of relative features to the households and to their displacement's habits and to their choices among public and privet transport modes. A comparison between this survey results and that of the 1996's is made. A pricing scheme is also given according to the tram capacity. (The Rabat-Salé tramway is under construction right now and it will be operational beginning 2010).

Keywords: Matrix O/D, Theory of pricing, Urban transport survey.

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2547 Limits of Phase Modulated Frequency Shifted Holographic Vibrometry at Low Amplitudes of Vibrations

Authors: Pavel Psota, Vít Lédl, Jan Václavík, Roman Doleček, Pavel Mokrý, Petr Vojtíšek

Abstract:

This paper presents advanced time average digital holography by means of frequency shift and phase modulation. This technique can measure amplitudes of vibrations at ultimate dynamic range while the amplitude distribution evaluation is done independently in every pixel. The main focus of the paper is to gain insight into behavior of the method at low amplitudes of vibrations. In order to reach that, a set of experiments was performed. Results of the experiments together with novel noise suppression show the limit of the method to be below 0.1 nm.

Keywords: Acousto-optical modulator, digital holography, low amplitudes, vibrometry.

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2546 Orders Preparation and Control on the Productive Process Efficiency Preparation

Authors: F. Charrua-Santos, S. Dias, J. Matias, F. Brójo, S. Azevedo.

Abstract:

The main objective of this paper is to analyse the influence of preparation and control of orders on performance. The focused activities explored in this research are: procurement, production and distribution. These changes in performance were obtained through improvement of the supply chain. It is proved using all the company activities that it is possible to increase de efficiency and do services in an adequate way, placing the products in the market efficiently. For that, it was explored the importance of the supply chain, with privilege to the practical environment and the quantification of the obtained results.

Keywords: Competitiveness, Order Preparation and Control, Procurement Process and Operations Efficiency, Supply Chain Global Costs

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2545 Coverage Strategies for Wireless Sensor Networks

Authors: Nor Azlina Ab. Aziz, Kamarulzaman Ab. Aziz, Wan Zakiah Wan Ismail

Abstract:

Coverage is one of the main research interests in wireless sensor networks (WSN), it is used to determine the quality of service (QoS) of the networks. Therefore this paper aims to review the common strategies use in solving coverage problem in WSN. The strategies studied are used during deployment phase where the coverage is calculated based on the placement of the sensors on the region of interest (ROI). The strategies reviewed are categorized into three groups based on the approaches used, namely; force based, grid based or computational geometry based approach.

Keywords: Computational geometry, coverage, Delaunay triangulation, force, grid, Voronoi diagram, wireless sensor networks.

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2544 Detection of Transgenes in Cotton (Gossypium hirsutum L.) by Using Biotechnology/Molecular Biological Techniques

Authors: Ahmad Ali Shahid, Muhammad Shakil Shaukat, Kamran Shehzad Bajwa, Abdul Qayyum Rao, Tayyab Husnain

Abstract:

Agriculture is the backbone of economy of Pakistan and cotton is the major agricultural export and supreme source of raw fiber for our textile industry. To combat severe problems of insect and weed, combination of three genes namely Cry1Ac, Cry2A and EPSPS genes was transferred in locally cultivated cotton variety MNH-786 with the use of Agrobacterium mediated genetic transformation. The present study focused on the molecular screening of transgenic cotton plants at T3 generation in order to confirm integration and expression of all three genes (Cry1Ac, Cry2A and EPSP synthase) into the cotton genome. Initially, glyphosate spray assay was used for screening of transgenic cotton plants containing EPSP synthase gene at T3 generation. Transgenic cotton plants which were healthy and showed no damage on leaves were selected after 07 days of spray. For molecular analysis of transgenic cotton plants in the laboratory, the genomic DNA of these transgenic cotton plants were isolated and subjected to amplification of the three genes. Thus, seventeen out of twenty (Cry1Ac gene), ten out of twenty (Cry2A gene) and all twenty (EPSP synthase gene) were produced positive amplification. On the base of PCR amplification, ten transgenic plant samples were subjected to protein expression analysis through ELISA. The results showed that eight out of ten plants were actively expressing the three transgenes. Real-time PCR was also done to quantify the mRNA expression levels of Cry1Ac and EPSP synthase gene. Finally, eight plants were confirmed for the presence and active expression of all three genes at T3 generation.

Keywords: Agriculture, Cotton, Transformation, Cry Genes, ELISA and PCR.

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2543 Conductivity and Selection of Copper Clad Steel Wires for Grounding Applications

Authors: George Eduful, Kingsford J. A. Atanga

Abstract:

Copper clad steel wire (CCS) is primarily used for grounding applications to reduce the high incidence of copper ground conductor theft in electrical installations. The cross sectional area of the CCS is selected by relating the diameter equivalence to a copper conductor. The main difficulty is how to use a simple analytical relation to determine the right conductivity of CCS for a particular application. The use of Eddy-Current instrument for measuring conductivity is known but in most cases, the instrument is not readily available. The paper presents a simplified approach on how to size and determine CCS conductivity for a given application.

Keywords: Copper clad steel wire, conductivity, grounding, skin effect.

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