Search results for: method and laboratory bias
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 8750

Search results for: method and laboratory bias

7850 Trajectory Planning Design Equations and Control of a 4 - axes Stationary Robotic Arm

Authors: T.C. Manjunath,

Abstract:

This paper features the trajectory planning design of a indigenously developed 4-Axis SCARA robot which is used for doing successful robotic manipulation task in the laboratory. Once, a trajectory is being designed and given as input to the robot, the robot's gripper tip moves along that specified trajectory. Trajectories have to be designed in the work space only. The main idea of this paper is to design a continuous path trajectory model for the indigenously developed SCARA robot arm during its maneuvering from one point to another point (during pick and place operations) in a workspace avoiding all the obstacles in its path of motion.

Keywords: SCARA, Trajectory, Planning.

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7849 Numerical Simulation of Fluid Structure Interaction Using Two-Way Method

Authors: Samira Laidaoui, Mohammed Djermane, Nazihe Terfaya

Abstract:

The fluid-structure coupling is a natural phenomenon which reflects the effects of two continuums: fluid and structure of different types in the reciprocal action on each other, involving knowledge of elasticity and fluid mechanics. The solution for such problems is based on the relations of continuum mechanics and is mostly solved with numerical methods. It is a computational challenge to solve such problems because of the complex geometries, intricate physics of fluids, and complicated fluid-structure interactions. The way in which the interaction between fluid and solid is described gives the largest opportunity for reducing the computational effort. In this paper, a problem of fluid structure interaction is investigated with two-way coupling method. The formulation Arbitrary Lagrangian-Eulerian (ALE) was used, by considering a dynamic grid, where the solid is described by a Lagrangian formulation and the fluid by a Eulerian formulation. The simulation was made on the ANSYS software.

Keywords: ALE, coupling, FEM, fluid-structure interaction, one-way method, two-way method.

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7848 An Improved Illumination Normalization based on Anisotropic Smoothing for Face Recognition

Authors: Sanghoon Kim, Sun-Tae Chung, Souhwan Jung, Seongwon Cho

Abstract:

Robust face recognition under various illumination environments is very difficult and needs to be accomplished for successful commercialization. In this paper, we propose an improved illumination normalization method for face recognition. Illumination normalization algorithm based on anisotropic smoothing is well known to be effective among illumination normalization methods but deteriorates the intensity contrast of the original image, and incurs less sharp edges. The proposed method in this paper improves the previous anisotropic smoothing-based illumination normalization method so that it increases the intensity contrast and enhances the edges while diminishing the effect of illumination variations. Due to the result of these improvements, face images preprocessed by the proposed illumination normalization method becomes to have more distinctive feature vectors (Gabor feature vectors) for face recognition. Through experiments of face recognition based on Gabor feature vector similarity, the effectiveness of the proposed illumination normalization method is verified.

Keywords: Illumination Normalization, Face Recognition, Anisotropic smoothing, Gabor feature vector.

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7847 Design of Angular Estimator of Inertial Sensor Using the Least Square Method

Authors: Ji Hoon Kim, Hyung Gi Min, Jae Dong Cho, Jae Hoon Jang, Sung-Ha Kwon, Eun Tae Jeung

Abstract:

Since MEMS gyro sensors measure not angle of rotation but angular rate, an estimator is designed to estimate the angles in many applications. Gyro and accelerometer are used to improve estimating accuracy of the angle. This paper presents a method of finding filter coefficients of the well-known estimator which is to get rotation angles from gyro and accelerometer data. In order to verify the performance of our method, the estimated angle is compared with the encoder output in a rotary pendulum system.

Keywords: gyro, accelerometer, estimator, least square.

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7846 Consideration a Novel Manner for Data Sending Quality in Heterogeneous Radio Networks

Authors: Mohammadreza Amini, Omid Moradtalab, Ebadollah Zohrevandi

Abstract:

In real-time networks a large number of application programs are relying on video data and heterogeneous data transmission techniques. The aim of this research is presenting a method for end-to-end vouch quality service in surface applicationlayer for sending video data in comparison form in wireless heterogeneous networks. This method tries to improve the video sending over the wireless heterogeneous networks with used techniques in surface layer, link and application. The offered method is showing a considerable improvement in quality observing by user. In addition to this, other specifications such as shortage of data load that had require to resending and limited the relation period length to require time for second data sending, help to be used the offered method in the wireless devices that have a limited energy. The presented method and the achieved improvement is simulated and presented in the NS-2 software.

Keywords: Heterogeneous wireless networks, adaptation mechanism, multi-level, Handoff, stop mechanism, graceful degrades, application layer.

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7845 Power Generation from Sewage by a Micro-Hydraulic Turbine

Authors: Tomomi Uchiyama, Tomoko Okayama, Yukio Ide

Abstract:

This study is concerned with the development of a micro-hydraulic turbine for power generation installed in sewer pipes. The runner has a circular hollow around the central (rotating) axis so that solid materials included in water can be easily flow through the runner without blocking the turbine. The laboratory experiments are also conducted. The hollow is very effective to make polyester fibers pass through the turbine. The guide vane is useful to heighten the turbine performance. But it is easily blocked by the fibers, making the turbine lose the function.

Keywords: Generation of electricity, micro-hydraulic turbine, sewage, sewer pipe.

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7844 Hydrological Modeling of Watersheds Using the Only Corresponding Competitor Method: The Case of M’Zab Basin, South East Algeria

Authors: Oulad Naoui Noureddine, Cherif ELAmine, Djehiche Abdelkader

Abstract:

Water resources management includes several disciplines; the modeling of rainfall-runoff relationship is the most important discipline to prevent natural risks. There are several models to study rainfall-runoff relationship in watersheds. However, the majority of these models are not applicable in all basins of the world.  In this study, a new stochastic method called The Only Corresponding Competitor method (OCC) was used for the hydrological modeling of M’ZAB   Watershed (South East of Algeria) to adapt a few empirical models for any hydrological regime.  The results obtained allow to authorize a certain number of visions, in which it would be interesting to experiment with hydrological models that improve collectively or separately the data of a catchment by the OCC method.

Keywords: Empirical model, modeling, OCC, rainfall-runoff relationship.

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7843 Method of Parameter Calibration for Error Term in Stochastic User Equilibrium Traffic Assignment Model

Authors: Xiang Zhang, David Rey, S. Travis Waller

Abstract:

Stochastic User Equilibrium (SUE) model is a widely used traffic assignment model in transportation planning, which is regarded more advanced than Deterministic User Equilibrium (DUE) model. However, a problem exists that the performance of the SUE model depends on its error term parameter. The objective of this paper is to propose a systematic method of determining the appropriate error term parameter value for the SUE model. First, the significance of the parameter is explored through a numerical example. Second, the parameter calibration method is developed based on the Logit-based route choice model. The calibration process is realized through multiple nonlinear regression, using sequential quadratic programming combined with least square method. Finally, case analysis is conducted to demonstrate the application of the calibration process and validate the better performance of the SUE model calibrated by the proposed method compared to the SUE models under other parameter values and the DUE model.

Keywords: Parameter calibration, sequential quadratic programming, Stochastic User Equilibrium, traffic assignment, transportation planning.

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7842 Efficient Solution for a Class of Markov Chain Models of Tandem Queueing Networks

Authors: Chun Wen, Tingzhu Huang

Abstract:

We present a new numerical method for the computation of the steady-state solution of Markov chains. Theoretical analyses show that the proposed method, with a contraction factor α, converges to the one-dimensional null space of singular linear systems of the form Ax = 0. Numerical experiments are used to illustrate the effectiveness of the proposed method, with applications to a class of interesting models in the domain of tandem queueing networks.

Keywords: Markov chains, tandem queueing networks, convergence, effectiveness.

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7841 Integral Tracking Control for a Piezoelectric Actuator System

Authors: J. H. Park, S. C. Jeong, J. H. Koo, H. Y. Jung, S. M. Lee

Abstract:

We propose an integral tracking control method for a piezoelectric actuator system. The proposed method achieves the output tracking without requiring any hysteresis observer or schemes to compensate the hysteresis effect. With the proposed control law, the system is converted into the standard singularly perturbed model. Using Tikhonov-s theorem, we guarantee that the tracking error can be reduced to arbitrarily small bound. A numerical example is given to illustrate the effectiveness of our proposed method.

Keywords: Piezoelectric actuator, tracking control, hysteresis effect.

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7840 Loudspeaker Parameters Inverse Problem for Improving Sound Frequency Response Simulation

Authors: Y. T. Tsai, Jin H. Huang

Abstract:

The sound pressure level (SPL) of the moving-coil loudspeaker (MCL) is often simulated and analyzed using the lumped parameter model. However, the SPL of a MCL cannot be simulated precisely in the high frequency region, because the value of cone effective area is changed due to the geometry variation in different mode shapes, it is also related to affect the acoustic radiation mass and resistance. Herein, the paper presents the inverse method which has a high ability to measure the value of cone effective area in various frequency points, also can estimate the MCL electroacoustic parameters simultaneously. The proposed inverse method comprises the direct problem, adjoint problem, and sensitivity problem in collaboration with nonlinear conjugate gradient method. Estimated values from the inverse method are validated experimentally which compared with the measured SPL curve result. Results presented in this paper not only improve the accuracy of lumped parameter model but also provide the valuable information on loudspeaker cone design.

Keywords: Inverse problem, cone effective area, loudspeaker, nonlinear conjugate gradient method.

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7839 Design of Stable IIR Digital Filters with Specified Group Delay Errors

Authors: Yasunori Sugita, Toshinori Yoshikawa

Abstract:

The design problem of Infinite Impulse Response (IIR) digital filters is usually expressed as the minimization problem of the complex magnitude error that includes both the magnitude and phase information. However, the group delay of the filter obtained by solving such design problem may be far from the desired group delay. In this paper, we propose a design method of stable IIR digital filters with prespecified maximum group delay errors. In the proposed method, the approximation problems of the magnitude-phase and group delay are separately defined, and these two approximation problems are alternately solved using successive projections. As a result, the proposed method can design the IIR filters that satisfy the prespecified allowable errors for not only the complex magnitude but also the group delay by alternately executing the coefficient update for the magnitude-phase and the group delay approximation. The usefulness of the proposed method is verified through some examples.

Keywords: Filter design, Group delay approximation, Stable IIRfilters, Successive projection method.

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7838 Determining the Best Method of Stability Landslide by Using of DSS (Case Study: Landslide in Hasan Salaran, Kurdistan Province in Iran)

Authors: S. Kamyabi, M. Salari, H. Shahabi

Abstract:

One of the processes of slope that occurs every year in Iran and some parts of world and cause a lot of criminal and financial harms is called landslide. They are plenty of method to stability landslide in soil and rock slides. The use of the best method with the least cost and in the shortest time is important for researchers. In this research, determining the best method of stability is investigated by using of Decision Support systems. DSS is made for this purpose and was used (for Hasan Salaran area in Kurdistan). Field study data from topography, slope, geology, geometry of landslide and the related features was used. The related data entered decision making managements programs (DSS) (ALES).Analysis of mass stability indicated the instability potential at present. Research results show that surface and sub surface drainage the best method of stabilizing. Analysis of stability shows that acceptable increase in security coefficient is a consequence of drainage.

Keywords: Landslide, Decision Support systems, stability, Hasan Salaran landslide, Kurdistan province, Iran.

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7837 Finite Volume Method for Flow Prediction Using Unstructured Meshes

Authors: Juhee Lee, Yongjun Lee

Abstract:

In designing a low-energy-consuming buildings, the heat transfer through a large glass or wall becomes critical. Multiple layers of the window glasses and walls are employed for the high insulation. The gravity driven air flow between window glasses or wall layers is a natural heat convection phenomenon being a key of the heat transfer. For the first step of the natural heat transfer analysis, in this study the development and application of a finite volume method for the numerical computation of viscous incompressible flows is presented. It will become a part of the natural convection analysis with high-order scheme, multi-grid method, and dual-time step in the future. A finite volume method based on a fully-implicit second-order is used to discretize and solve the fluid flow on unstructured grids composed of arbitrary-shaped cells. The integrations of the governing equation are discretised in the finite volume manner using a collocated arrangement of variables. The convergence of the SIMPLE segregated algorithm for the solution of the coupled nonlinear algebraic equations is accelerated by using a sparse matrix solver such as BiCGSTAB. The method used in the present study is verified by applying it to some flows for which either the numerical solution is known or the solution can be obtained using another numerical technique available in the other researches. The accuracy of the method is assessed through the grid refinement.

Keywords: Finite volume method, fluid flow, laminar flow, unstructured grid.

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7836 Aircraft Selection Problem Using Decision Uncertainty Distance in Fuzzy Multiple Criteria Decision Making Analysis

Authors: C. Ardil

Abstract:

Aircraft have different capabilities and specifications according to the required strategic goals and objectives in operations. With various types on the market with different aircraft characteristics, it becomes difficult to select a suitable aircraft for certain operations and requirements. The entropy weighting method (EWM) is a useful, highly consistent, and reliable method for obtaining the weights of the criteria and is worth integrating with the decision uncertainty distance (DUD) method, which is more applicable and requires less computation than other methods. An illustrative example is presented to demonstrate the validity and usability of the proposed methodology. Comparing the ranking results matches the distance-based approach, which is the technique for order preference by similarity to ideal solution (TOPSIS) method, which shows the robustness of the entropy DUD hybrid method. Validity analysis shows that the proposed hybrid multiple criteria decision-making analysis (MCDMA) methodology is quantitatively stable and reliable.

Keywords: aircraft selection, decision uncertainty distance (DUD), multiple criteria decision making analysis, MCDMA, TOPSIS

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7835 Key Frames Extraction for Sign Language Video Analysis and Recognition

Authors: Jaroslav Polec, Petra Heribanová, Tomáš Hirner

Abstract:

In this paper we proposed a method for finding video frames representing one sign in the finger alphabet. The method is based on determining hands location, segmentation and the use of standard video quality evaluation metrics. Metric calculation is performed only in regions of interest. Sliding mechanism for finding local extrema and adaptive threshold based on local averaging is used for key frames selection. The success rate is evaluated by recall, precision and F1 measure. The method effectiveness is compared with metrics applied to all frames. Proposed method is fast, effective and relatively easy to realize by simple input video preprocessing and subsequent use of tools designed for video quality measuring.

Keywords: Key frame, video, quality, metric, MSE, MSAD, SSIM, VQM, sign language, finger alphabet.

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7834 Analytical Based Truncation Principle of Higher-Order Solution for a x1/3 Force Nonlinear Oscillator

Authors: Md. Alal Hosen

Abstract:

In this paper, a modified harmonic balance method based an analytical technique has been developed to determine higher-order approximate periodic solutions of a conservative nonlinear oscillator for which the elastic force term is proportional to x1/3. Usually, a set of nonlinear algebraic equations is solved in this method. However, analytical solutions of these algebraic equations are not always possible, especially in the case of a large oscillation. In this article, different parameters of the same nonlinear problems are found, for which the power series produces desired results even for the large oscillation. We find a modified harmonic balance method works very well for the whole range of initial amplitudes, and the excellent agreement of the approximate frequencies and periodic solutions with the exact ones has been demonstrated and discussed. Besides these, a suitable truncation formula is found in which the solution measures better results than existing solutions. The method is mainly illustrated by the x1/3 force nonlinear oscillator but it is also useful for many other nonlinear problems.

Keywords: Approximate solutions, Harmonic balance method, Nonlinear oscillator, Perturbation.

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7833 Statically Fused Unbiased Converted Measurements Kalman Filter

Authors: Zhengkun Guo, Yanbin Li, Wenqing Wang, Bo Zou

Abstract:

Active radar and sonar systems often report Doppler measurements in addition to the position measurements such as range and bearing. The tracker can perform better by making full use of the Doppler measurements. However, due to the high nonlinearity of the Doppler measurements with respect to the target state in the Cartesian coordinate systems, those measurements are not always fully exploited. This paper mainly focuses on dealing with the Doppler measurements as well as the position measurements in Polar coordinates. The Statically Fused Converted Position and Doppler Measurements Kalman Filter (SF-CMKF) with additive debiased measurement conversion has been presented. However, the exact compensation for the bias of the measurement conversion are multiplicative and depend on the statistics of the cosine of the angle measurement errors. As a result, the consistency and performance of the SF-CMKF may be suboptimal in the large angle error situations. In this paper, the multiplicative unbiased position and Doppler measurement conversion for two-dimensional (Polar-to-Cartesian) tracking are derived, and the SF-CMKF is improved by using those conversion. Monte Carlo simulations are presented to demonstrate the statistic consistency of the multiplicative unbiased conversion and the superior performance of the modified SF-CMKF (SF-UCMKF).

Keywords: Measurement conversion, Doppler, Kalman filter, estimation, tracking.

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7832 Slip Suppression of Electric Vehicles using Model Predictive PID Controller

Authors: Tohru Kawabe

Abstract:

In this paper, a new model predictive PID controller design method for the slip suppression control of EVs (electric vehicles) is proposed. The proposed method aims to improve the maneuverability and the stability of EVs by controlling the wheel slip ratio. The optimal control gains of PID framework are derived by the model predictive control (MPC) algorithm. There also include numerical simulation results to demonstrate the effectiveness of the method.

Keywords: Model Predictive Control, PID controller, Electric Vehicle, Slip suppression

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7831 Model Order Reduction for Frequency Response and Effect of Order of Method for Matching Condition

Authors: Aref Ghafouri, Mohammad Javad Mollakazemi, Farhad Asadi

Abstract:

In this paper, model order reduction method is used for approximation in linear and nonlinearity aspects in some experimental data. This method can be used for obtaining offline reduced model for approximation of experimental data and can produce and follow the data and order of system and also it can match to experimental data in some frequency ratios. In this study, the method is compared in different experimental data and influence of choosing of order of the model reduction for obtaining the best and sufficient matching condition for following the data is investigated in format of imaginary and reality part of the frequency response curve and finally the effect and important parameter of number of order reduction in nonlinear experimental data is explained further.

Keywords: Frequency response, Order of model reduction, frequency matching condition.

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7830 Simulation of PM10 Source Apportionment at An Urban Site in Southern Taiwan by a Gaussian Trajectory Model

Authors: Chien-Lung Chen, Jeng-Lin Tsai, Feng-Chao Chung, Su-Ching Kuo, Kuo-Hsin Tseng, Pei-Hsuan Kuo, Li-Ying Hsieh, Ying I. Tsai

Abstract:

This study applied the Gaussian trajectory transfer-coefficient model (GTx) to simulate the particulate matter concentrations and the source apportionments at Nanzih Air Quality Monitoring Station in southern Taiwan from November 2007 to February 2008. The correlation coefficient between the observed and the calculated daily PM10 concentrations is 0.5 and the absolute bias of the PM10 concentrations is 24%. The simulated PM10 concentrations matched well with the observed data. Although the emission rate of PM10 was dominated by area sources (58%), the results of source apportionments indicated that the primary sources for PM10 at Nanzih Station were point sources (42%), area sources (20%) and then upwind boundary concentration (14%). The obvious difference of PM10 source apportionment between episode and non-episode days was upwind boundary concentrations which contributed to 20% and 11% PM10 sources, respectively. The gas-particle conversion of secondary aerosol and long range transport played crucial roles on the PM10 contribution to a receptor.

Keywords: back trajectory model, particulate matter, sourceapportionment

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7829 Heuristic Method for Judging the Computational Stability of the Difference Schemes of the Biharmonic Equation

Authors: Guang Zeng, Jin Huang, Zicai Li

Abstract:

In this paper, we research the standard 13-point difference schemes for solving the biharmonic equation. Heuristic method is applied to judging the stability of multi-level difference schemes of the biharmonic equation. It is showed that the standard 13-point difference schemes are stable.

Keywords: Finite-difference equation, computational stability, hirt method.

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7828 Application of KL Divergence for Estimation of Each Metabolic Pathway Genes

Authors: Shohei Maruyama, Yasuo Matsuyama, Sachiyo Aburatani

Abstract:

Development of a method to estimate gene functions is an important task in bioinformatics. One of the approaches for the annotation is the identification of the metabolic pathway that genes are involved in. Since gene expression data reflect various intracellular phenomena, those data are considered to be related with genes’ functions. However, it has been difficult to estimate the gene function with high accuracy. It is considered that the low accuracy of the estimation is caused by the difficulty of accurately measuring a gene expression. Even though they are measured under the same condition, the gene expressions will vary usually. In this study, we proposed a feature extraction method focusing on the variability of gene expressions to estimate the genes' metabolic pathway accurately. First, we estimated the distribution of each gene expression from replicate data. Next, we calculated the similarity between all gene pairs by KL divergence, which is a method for calculating the similarity between distributions. Finally, we utilized the similarity vectors as feature vectors and trained the multiclass SVM for identifying the genes' metabolic pathway. To evaluate our developed method, we applied the method to budding yeast and trained the multiclass SVM for identifying the seven metabolic pathways. As a result, the accuracy that calculated by our developed method was higher than the one that calculated from the raw gene expression data. Thus, our developed method combined with KL divergence is useful for identifying the genes' metabolic pathway.

Keywords: Metabolic pathways, gene expression data, microarray, Kullback–Leibler divergence, KL divergence, support vector machines, SVM, machine learning.

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7827 Design of Speed and Power Control System for Wind Turbine with Reference Tracking Method

Authors: H. Ghanbari, H. Nikbakht, A. Zahedi, M. Ghanbari

Abstract:

This paper is focusing on designing a control system for wind turbine which can control the speed and output power according to arbitrary algorithm. Reference Tracking Method is used to control the turbine spinning speed in order to increase its output energy.

Keywords: Wind Turbine, Simulink, Reference Tracking Method.

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7826 On-line Identification of Continuous-time Hammerstein Systems via RBF Networks and Immune Algorithm

Authors: Tomohiro Hachino, Kengo Nagatomo, Hitoshi Takata

Abstract:

This paper deals with an on-line identification method of continuous-time Hammerstein systems by using the radial basis function (RBF) networks and immune algorithm (IA). An unknown nonlinear static part to be estimated is approximately represented by the RBF network. The IA is efficiently combined with the recursive least-squares (RLS) method. The objective function for the identification is regarded as the antigen. The candidates of the RBF parameters such as the centers and widths are coded into binary bit strings as the antibodies and searched by the IA. On the other hand, the candidates of both the weighting parameters of the RBF network and the system parameters of the linear dynamic part are updated by the RLS method. Simulation results are shown to illustrate the proposed method.

Keywords: Continuous-time System, Hammerstein System, OnlineIdentification, Immune Algorithm, RBF network.

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7825 Vendor Selection and Supply Quotas Determination by using Revised Weighting Method and Multi-Objective Programming Methods

Authors: Tunjo Perić, Marin Fatović

Abstract:

In this paper a new methodology for vendor selection and supply quotas determination (VSSQD) is proposed. The problem of VSSQD is solved by the model that combines revised weighting method for determining the objective function coefficients, and a multiple objective linear programming (MOLP) method based on the cooperative game theory for VSSQD. The criteria used for VSSQD are: (1) purchase costs and (2) product quality supplied by individual vendors. The proposed methodology has been tested on the example of flour purchase for a bakery with two decision makers.

Keywords: Cooperative game theory, multiple objective linear programming, revised weighting method, vendor selection.

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7824 Design, Construction and Performance Evaluation of a HPGe Detector Shield

Authors: M. Sharifi, M. Mirzaii, F. Bolourinovin, H. Yousefnia, M. Akbari, K. Yousefi-Mojir

Abstract:

A multilayer passive shield composed of low-activity lead (Pb), copper (Cu), tin (Sn) and iron (Fe) was designed and manufactured for a coaxial HPGe detector placed at a surface laboratory for reducing background radiation and radiation dose to the personnel. The performance of the shield was evaluated and efficiency curves of the detector were plotted by using of various standard sources in different distances. Monte Carlo simulations and a set of TLD chips were used for dose estimation in two distances of 20 and 40 cm. The results show that the shield reduced background spectrum and the personnel dose more than 95%.

Keywords: HPGe shield, background count, personnel dose, efficiency curve.

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7823 Geometric Operators in Decision Making with Minimization of Regret

Authors: José M. Merigó, Montserrat Casanovas

Abstract:

We study different types of aggregation operators and the decision making process with minimization of regret. We analyze the original work developed by Savage and the recent work developed by Yager that generalizes the MMR method creating a parameterized family of minimal regret methods by using the ordered weighted averaging (OWA) operator. We suggest a new method that uses different types of geometric operators such as the weighted geometric mean or the ordered weighted geometric operator (OWG) to generalize the MMR method obtaining a new parameterized family of minimal regret methods. The main result obtained in this method is that it allows to aggregate negative numbers in the OWG operator. Finally, we give an illustrative example.

Keywords: Decision making, Regret, Aggregation operators, OWA operator, OWG operator.

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7822 Screen of MicroRNA Targets in Zebrafish Using Heterogeneous Data Sources: A Case Study for Dre-miR-10 and Dre-miR-196

Authors: Yanju Zhang, Joost M. Woltering, Fons J. Verbeek

Abstract:

It has been established that microRNAs (miRNAs) play an important role in gene expression by post-transcriptional regulation of messengerRNAs (mRNAs). However, the precise relationships between microRNAs and their target genes in sense of numbers, types and biological relevance remain largely unclear. Dissecting the miRNA-target relationships will render more insights for miRNA targets identification and validation therefore promote the understanding of miRNA function. In miRBase, miRanda is the key algorithm used for target prediction for Zebrafish. This algorithm is high-throughput but brings lots of false positives (noise). Since validation of a large scale of targets through laboratory experiments is very time consuming, several computational methods for miRNA targets validation should be developed. In this paper, we present an integrative method to investigate several aspects of the relationships between miRNAs and their targets with the final purpose of extracting high confident targets from miRanda predicted targets pool. This is achieved by using the techniques ranging from statistical tests to clustering and association rules. Our research focuses on Zebrafish. It was found that validated targets do not necessarily associate with the highest sequence matching. Besides, for some miRNA families, the frequency of their predicted targets is significantly higher in the genomic region nearby their own physical location. Finally, in a case study of dre-miR-10 and dre-miR-196, it was found that the predicted target genes hoxd13a, hoxd11a, hoxd10a and hoxc4a of dre-miR- 10 while hoxa9a, hoxc8a and hoxa13a of dre-miR-196 have similar characteristics as validated target genes and therefore represent high confidence target candidates.

Keywords: MicroRNA targets validation, microRNA-target relationships, dre-miR-10, dre-miR-196.

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7821 Laser-Ultrasonic Method for Measuring the Local Elastic Moduli of Porous Isotropic Composite Materials

Authors: Alexander A. Karabutov, Natalia B. Podymova, Elena B. Cherepetskaya, Vladimir A. Makarov, Yulia G. Sokolovskaya

Abstract:

The laser-ultrasonic method is realized for quantifying the influence of porosity on the local Young’s modulus of isotropic composite materials. The method is based on a laser thermooptical method of ultrasound generation combined with measurement of the phase velocity of longitudinal and shear acoustic waves in samples. The main advantage of this method compared with traditional ultrasonic research methods is the efficient generation of short and powerful probing acoustic pulses required for reliable testing of ultrasound absorbing and scattering heterogeneous materials. Using as an example samples of a metal matrix composite with reinforcing microparticles of silicon carbide in various concentrations, it is shown that to provide an effective increase in Young’s modulus with increasing concentration of microparticles, the porosity of the final sample should not exceed 2%.

Keywords: Laser ultrasonic, longitudinal and shear ultrasonic waves, porosity, composite, local elastic moduli.

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