Search results for: mesoscopic perovskite solar cells
490 Experimental and Finite Element Analysis for Mechanics of Soil-Tool Interaction
Authors: A. Armin, R. Fotouhi, W. Szyszkowski
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In this paper a 3-D finite element (FE) investigation of soil-blade interaction is described. The effects of blade’s shape and rake angle are examined both numerically and experimentally. The soil is considered as an elastic-plastic granular material with non-associated Drucker-Prager material model. Contact elements with different properties are used to mimic soil-blade sliding and soil-soil cutting phenomena. A separation criterion is presented and a procedure to evaluate the forces acting on the blade is given and discussed in detail. Experimental results were derived from tests using soil bin facility and instruments at the University of Saskatchewan. During motion of the blade, load cells collect data and send them to a computer. The measured forces using load cells had noisy signals which are needed to be filtered. The FE results are compared with experimental results for verification. This technique can be used in blade shape optimization and design of more complicated blade’s shape.
Keywords: Finite element analysis, soil-blade contact modeling, blade force, experimental results.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1182489 A Robust Method for Finding Nearest-Neighbor using Hexagon Cells
Authors: Ahmad Attiq Al-Ogaibi, Ahmad Sharieh, Moh’d Belal Al-Zoubi, R. Bremananth
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In pattern clustering, nearest neighborhood point computation is a challenging issue for many applications in the area of research such as Remote Sensing, Computer Vision, Pattern Recognition and Statistical Imaging. Nearest neighborhood computation is an essential computation for providing sufficient classification among the volume of pixels (voxels) in order to localize the active-region-of-interests (AROI). Furthermore, it is needed to compute spatial metric relationships of diverse area of imaging based on the applications of pattern recognition. In this paper, we propose a new methodology for finding the nearest neighbor point, depending on making a virtually grid of a hexagon cells, then locate every point beneath them. An algorithm is suggested for minimizing the computation and increasing the turnaround time of the process. The nearest neighbor query points Φ are fetched by seeking fashion of hexagon holistic. Seeking will be repeated until an AROI Φ is to be expected. If any point Υ is located then searching starts in the nearest hexagons in a circular way. The First hexagon is considered be level 0 (L0) and the surrounded hexagons is level 1 (L1). If Υ is located in L1, then search starts in the next level (L2) to ensure that Υ is the nearest neighbor for Φ. Based on the result and experimental results, we found that the proposed method has an advantage over the traditional methods in terms of minimizing the time complexity required for searching the neighbors, in turn, efficiency of classification will be improved sufficiently.
Keywords: Hexagon cells, k-nearest neighbors, Nearest Neighbor, Pattern recognition, Query pattern, Virtually grid
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2802488 Identification of Differentially Expressed Gene(DEG) in Atherosclerotic Lesion by Annealing Control Primer (ACP)-Based Genefishing™ PCR
Authors: M. Maimunah, G. A. Froemming, H. Nawawi, M. I. Nafeeza, O. Effat, M. Y. Rosmadi, M. S. Mohamed Saifulaman
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Atherosclerosis was identified as a chronic inflammatory process resulting from interactions between plasma lipoproteins, cellular components (monocyte, macrophages, T lymphocytes, endothelial cells and smooth muscle cells) and the extracellular matrix of the arterial wall. Several types of genes were known to express during formation of atherosclerosis. This study is carried out to identify unknown differentially expressed gene (DEG) in atherogenesis. Rabbit’s aorta tissues were stained by H&E for histomorphology. GeneFishing™ PCR analysis was performed from total RNA extracted from the aorta tissues. The DNA fragment from DEG was cloned, sequenced and validated by Real-time PCR. Histomorphology showed intimal thickening in the aorta. DEG detected from ACP-41 was identified as cathepsin B gene and showed upregulation at week-8 and week-12 of atherogenesis. Therefore, ACP-based GeneFishing™ PCR facilitated identification of cathepsin B gene which was differentially expressed during development of atherosclerosis.
Keywords: Atherosclerosis, GeneFishing™ PCR, cathepsin B gene.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1956487 Effects of Allelochemical Gramine on Metabolic Activity and Ultrastructure of Cyanobacterium Microcystis aeruginosa
Authors: Y. Hong, H. Y. Hu, A. Sakoda, M. Sagehashi
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In this study, inhibition of Microcystis aeruginosa by antialgal alleochemical gramine, was studied by analyzing algal metabolic activity (represented by esterase and total dehydrogenase activities) and cell ultrastructure (showing morphological and ultrastructure alterations using transmission electron microscopy and DNA ladder analysis). After gramine exposure, esterase and total dehydrogenase activities were increased firstly but decreased later. In contrast with the controls, the cells exposed to gramine showed apparent ultrastructure alterations with thylakoids in breakage, phycobilins in decrease, lipid and cyanophycin granules abundant firstly but dissolved afterwards, DNA in fragementation. The occurrence of increase of metabolic activity and specific granules reflected that the resistance of cellular response to gramine was initiated. DNA fragementation associated with the increase of metabolic activity and specific granules hinted that gramine caused M. aeruginosa cells to initiate some morphotype of programmed cell death.Keywords: Allelochemical, gramine, metabolic activity, Microcystis aeruginosa, ultrastructure.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1783486 Study of the Oxidation Resistance of Coated AISI 441 Ferritic Stainless Steel for SOFCs
Authors: M. B. Limooei, Hadi Ebrahimifar, Sh. Hosseini
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Protective coatings that resist oxide scale growth and decrease chromium evaporation are necessary to make stainless steel interconnect materials for long-term durable operation of solid oxide fuel cells (SOFCs). In this study a layer of cobalt was electroplated on the surface of AISI 441 ferritic stainless steel which is used in solid oxide fuel cells for interconnect applications. The oxidation behavior of coated substrates was studied as a function of time at operating conditions of SOFCs. Cyclic oxidation has been also tested at 800ºC for 100 cycles. Cobalt coating during isothermal oxidation caused to the oxide growth resistance by limiting the outward diffusion of Cr cation and the inward diffusion of oxygen anion. Results of cyclic oxidation exhibited that coated substrates demonstrate an excellent resistance against the spallation and cracking.
Keywords: Oxidation resistance, full cell, Cobalt coating, ferritic stainless steel.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2032485 Effect of Pre-drying Treatments on Quality Characteristics of Dehydrated Tomato Slices
Authors: Sharareh Mohseni, Reihaneh Ahmadzadeh Ghavidel
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Tomato powder has good potential as substitute of tomato paste and other tomato products. In order to protect physicochemical properties and nutritional quality of tomato during dehydration process, investigation was carried out using different drying methods and pretreatments. Solar drier and continuous conveyor (tunnel) drier were used for dehydration where as calcium chloride (CaCl2), potassium metabisulphite (KMS), calcium chloride and potassium metabisulphite (CaCl2 +KMS), and sodium chloride (NaCl) selected for treatment.. lycopene content, dehydration ratio, rehydration ratio and non-enzymatic browning in addition to moisture, sugar and titrable acidity were studied. Results show that pre-treatment with CaCl2 and NaCl increased water removal and moisture mobility in tomato slices during drying of tomatoes. Where CaCl2 used along with KMS the NEB was recorded the least compared to other treatments and the best results were obtained while using the two chemicals in combination form. Storage studies in LDPE polymeric and metalized polyesters films showed less changes in the products packed in metallized polyester pouches and even after 6 months lycopene content did not decrease more than 20% as compared to the control sample and provide extension of shelf life in acceptable condition for 6 months. In most of the quality characteristics tunnel drier samples presented better values in comparison to solar drier.
Keywords: Dehydration, Tomato powder, Lycopene, Browning
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2321484 Signal and Thermodynamic Analysis for Evaluation of Thermal and Power of Gas Turbine-Solid Oxide Fuel Cell Hybrid System
Authors: R. Mahjoub, K. Maghsoudi Mehraban
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In recent years, solid oxide fuel cells have been used as one of the main technologies for the production of electrical energy with high-efficiency ratio, which is used hydrogen and other hydrocarbons as fuels. The fuel cell technology can be used either alone or in hybrid gas turbines systems. In this study, thermodynamics analysis for GT-SOFC hybrid system is developed, and then mass balance and exergy equations have been applied not only on the process but also on the individual components of the hybrid system, which enable us to estimate the thermal efficiency of the hybrid systems. Furthermore, various sources of irreversibility in the solid oxide fuel cell system are discussed, and modeling and parametric analyses like heat and pressure are carried out. This study enables us to consider the irreversible effects of solid oxide fuel cells, and also it leads to the specification of efficiency of the system accurately. Next in the study, both methane and hydrogen as a fuel for SOFC are used and implemented, and finally, our results are compared with other references.
Keywords: hybrid system, gas turbine, entropy and exergy analysis, irreversibility analysis
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 494483 Blood Cell Dynamics in a Simple Shear Flow using an Implicit Fluid-Structure Interaction Method Based on the ALE Approach
Authors: Choeng-Ryul Choi, Chang-Nyung Kim, Tae-Hyub Hong
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A numerical method is developed for simulating the motion of particles with arbitrary shapes in an effectively infinite or bounded viscous flow. The particle translational and angular motions are numerically investigated using a fluid-structure interaction (FSI) method based on the Arbitrary-Lagrangian-Eulerian (ALE) approach and the dynamic mesh method (smoothing and remeshing) in FLUENT ( ANSYS Inc., USA). Also, the effects of arbitrary shapes on the dynamics are studied using the FSI method which could be applied to the motions and deformations of a single blood cell and multiple blood cells, and the primary thrombogenesis caused by platelet aggregation. It is expected that, combined with a sophisticated large-scale computational technique, the simulation method will be useful for understanding the overall properties of blood flow from blood cellular level (microscopic) to the resulting rheological properties of blood as a mass (macroscopic).Keywords: Blood Flow, Fluid-Structure Interaction (FSI), Micro-Channels, Arbitrary Shapes, Red Blood Cells (RBCs)
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2310482 Effect of Wavy Leading-Edges on Wings in Different Planetary Atmospheres
Authors: Vatasta Koul, Ayush Gupta, Vaibhav Sharma, Rajesh Yadav
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Today we are unmarking the secrets of the universe by exploring different stars and planets and most of the space exploration is done by unmanned space robots. In addition to our planet Earth, there are pieces of evidence that show other astronomical objects in our solar system such as Venus, Mars, Saturn’s moon Titan and Uranus support the flight of fixed wing air vehicles. In this paper, we take forward the concept of presence of large rounded tubercles along the leading edge of a wing and use it as a passive flow control device that will help in improving its aerodynamic performance and maneuverability. Furthermore, in this research, aerodynamic measurements and performance analysis of wavy leading tubercles on the fixed wings at 5-degree angle of attack are carried out after determination of the flow conditions on the selected planetary bodies. Wavelength and amplitude for the sinusoidal modifications on the leading edge are analyzed and simulations are carried out for three-dimensional NACA 0012 airfoil maintaining unity AR (Aspect Ratio). Tubercles have consistently demonstrated the ability to delay and decrease the severity of stall as per the studies were done in the Earth’s atmosphere. Implementing the same design on the leading edges of Micro-Air Vehicles (MAVs) and UAVs could make these aircrafts more stable over a greater range of angles of attack in different planetary environments of our solar system.
Keywords: Amplitude, NACA0012, tubercles, unmanned space robots.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 659481 Studies on Physiochemical Properties of Tomato Powder as Affected by Different Dehydration Methods and Pretreatments
Authors: Reihaneh Ahmadzadeh Ghavidel, Mehdi Ghiafeh Davoodi
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Tomato powder has good potential as substitute of tomato paste and other tomato products. In order to protect physicochemical properties and nutritional quality of tomato during dehydration process, investigation was carried out using different drying methods and pretreatments. Solar drier and continuous conveyor (tunnel) drier were used for dehydration where as calcium chloride (CaCl2), potassium metabisulphite (KMS), calcium chloride and potassium metabisulphite (CaCl2 +KMS), and sodium chloride (NaCl) selected for treatment.. lycopene content, dehydration ratio, rehydration ratio and non-enzymatic browning in addition to moisture, sugar and titrable acidity were studied. Results show that pre-treatment with CaCl2 and NaCl increased water removal and moisture mobility in tomato slices during drying of tomatoes. Where CaCl2 used along with KMS the NEB was recorded the least compared to other treatments and the best results were obtained while using the two chemicals in combination form. Storage studies in LDPE polymeric and metalized polyesters films showed less changes in the products packed in metallized polyester pouches and even after 6 months lycopene content did not decrease more than 20% as compared to the control sample and provide extension of shelf life in acceptable condition for 6 months. In most of the quality characteristics tunnel drier samples presented better values in comparison to solar drier.
Keywords: Dehydration, Tomato powder, Lycopene, Browning
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 4026480 Generalized Maximal Ratio Combining as a Supra-optimal Receiver Diversity Scheme
Authors: Jean-Pierre Dubois, Rania Minkara, Rafic Ayoubi
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Maximal Ratio Combining (MRC) is considered the most complex combining technique as it requires channel coefficients estimation. It results in the lowest bit error rate (BER) compared to all other combining techniques. However the BER starts to deteriorate as errors are introduced in the channel coefficients estimation. A novel combining technique, termed Generalized Maximal Ratio Combining (GMRC) with a polynomial kernel, yields an identical BER as MRC with perfect channel estimation and a lower BER in the presence of channel estimation errors. We show that GMRC outperforms the optimal MRC scheme in general and we hereinafter introduce it to the scientific community as a new “supraoptimal" algorithm. Since diversity combining is especially effective in small femto- and pico-cells, internet-associated wireless peripheral systems are to benefit most from GMRC. As a result, many spinoff applications can be made to IP-based 4th generation networks.
Keywords: Bit error rate, femto-internet cells, generalized maximal ratio combining, signal-to-scattering noise ratio.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2152479 Vibration Analysis of a Solar Powered UAV
Authors: Kevin Anderson, Sukhwinder Singh Sandhu, Nouh Anies, Shilpa Ravichandra, Steven Dobbs, Donald Edberg
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This paper presents the results of a Finite Element based vibration analysis of a solar powered Unmanned Aerial Vehicle (UAV). The purpose of this paper was to quantify the free vibration, forced vibration response due to differing point inputs in order to predict the relative response magnitudes and frequencies at various wing locations of vibration induced power generators (magnet in coil) excited by gust and/or control surface pulse-decays used to help power the flight of the electric UAV. A Fluid Structure Interaction (FSI) study was performed in order to ascertain pertinent design stresses and deflections as well as aerodynamic parameters of the UAV airfoil. The 10 ft span airfoil is modeled using Mylar as the primary material. Results show that the free mode in bending is 4.8 Hz while the first forced bending mode is on range of 16.2 to 16.7 Hz depending on the location of excitation. The free torsional bending mode is 28.3 Hz, and the first forced torsional mode is range of 26.4 to 27.8 Hz, depending on the location of excitation. The FSI results predict the coefficients of aerodynamic drag and lift of 0.0052 and 0.077, respectively, which matches hand-calculations used to validate the Finite Element based results. FSI based maximum von Mises stresses and deflections were found to be 0.282 MPa and 3.4 mm, respectively. Dynamic pressures on the airfoil range from 1.04 to 1.23 kPa corresponding to velocity magnitudes in range of 22 to 66 m/s.Keywords: ANSYS, finite element, FSI, UAV, vibrations.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2751478 A Heat-Inducible Transgene Expression System for Gene Therapy
Authors: Masaki Yamaguchi, Akira Ito, Noriaki Okamoto, Yoshinori Kawabe, Masamichi Kamihira
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Heat-inducible gene expression vectors are useful for hyperthermia-induced cancer gene therapy, because the combination of hyperthermia and gene therapy can considerably improve the therapeutic effects. In the present study, we developed an enhanced heat-inducible transgene expression system in which a heat-shock protein (HSP) promoter and tetracycline-responsive transactivator were combined. When the transactivator plasmid containing the tetracycline-responsive transactivator gene was co-transfected with the reporter gene expression plasmid, a high level of heat-induced gene expression was observed compared with that using the HSP promoter without the transactivator. In vitro evaluation of the therapeutic effect using HeLa cells showed that heat-induced therapeutic gene expression caused cell death in a high percentage of these cells, indicating that this strategy is promising for cancer gene therapy.Keywords: Inducible gene expression, Gene therapy, Hyperthermia, Heat shock protein, Tetracycline transactivator.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2135477 Application of Genetic Engineering for Chromium Removal from Industrial Wastewater
Authors: N. K. Srivastava, M. K. Jha, I. D. Mall, Davinder Singh
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The treatment of the industrial wastewater can be particularly difficult in the presence of toxic compounds. Excessive concentration of Chromium in soluble form is toxic to a wide variety of living organisms. Biological removal of heavy metals using natural and genetically engineered microorganisms has aroused great interest because of its lower impact on the environment. Ralston metallidurans, formerly known as Alcaligenes eutrophus is a LProteobacterium colonizing industrial wastewater with a high content of heavy metals. Tris-buffered mineral salt medium was used for growing Alcaligenes eutrophus AE104 (pEBZ141). The cells were cultivated for 18 h at 30 oC in Tris-buffered mineral salt medium containing 3 mM disodium sulphate and 46 mM sodium gluconate as the carbon source. The cells were harvested by centrifugation, washed, and suspended in 10 mM Tris HCl, pH 7.0, containing 46 mM sodium gluconate, and 5 mM Chromium. Interaction among induction of chr resistance determinant, and chromate reduction have been demonstrated. Results of this study show that the above bacteria can be very useful for bioremediation of chromium from industrial wastewater.Keywords: Chromium, Genetic Engineering, IndustrialWastewater, Plasmid
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2341476 An Assessment of the Small Hydropower Potential of Sisakht Region of Yasuj
Authors: F. Boustani
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Energy generated by the force of water in hydropower can provide a more sustainable, non-polluting alternative to fossil fuels, along with other renewable sources of energy, such as wind, solar and tidal power, bio energy and geothermal energy. Small scale hydroelectricity in Iran is well suited for “off-grid" rural electricity applications, while other renewable energy sources, such as wind, solar and biomass, can be beneficially used as fuel for pumping groundwater for drinking and small scale irrigation in remote rural areas or small villages. Small Hydro Power plants in Iran have very low operating and maintenance costs because they consume no fossil or nuclear fuel and do not involve high temperature processes. The equipment is relatively simple to operate and maintain. Hydropower equipment can adjust rapidly to load changes. The extended equipment life provides significant economic advantages. Some hydroelectric plants installed 100 years ago still operate reliably. The Polkolo river is located on Karun basin at southwest of Iran. Situation and conditions of Polkolo river are evaluated for construction of small hydropower in this article. The topographical conditions and the existence of permanent water from springs provide the suitability to install hydroelectric power plants on the river Polkolo. The cascade plant consists of 9 power plants connected with each other and is having the total head as 1100m and discharge about 2.5cubic meter per second. The annual production of energy is 105.5 million kwh.Keywords: Hydropower potential, Iran, SHP, Yasuj.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1986475 Rice cDNA Encoding PROLM is Capable of Rescuing Salt Sensitive Yeast Phenotypes G19 and Axt3K from Salt Stress
Authors: Prasad Senadheera, Younousse Saidi, Frans JM Maathuis
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Rice seed expression (cDNA) library in the Lambda Zap 11® phage constructed from the developing grain 10-20 days after flowering was transformed into yeast for functional complementation assays in three salt sensitive yeast mutants S. cerevisiae strain CY162, G19 and Axt3K. Transformed cells of G19 and Axt3K with pYES vector with cDNA inserts showed enhance tolerance than those with empty pYes vector. Sequencing of the cDNA inserts revealed that they encode for the putative proteins with the sequence homologous to rice putative protein PROLM24 (Os06g31070), a prolamin precursor. Expression of this cDNA did not affect yeast growth in absence of salt. Axt3k and G19 strains expressing the PROLM24 were able to grow upto 400 mM and 600 mM of NaCl respectively. Similarly, Axt3k mutant with PROLM24 expression showed comparatively higher growth rate in the medium with excess LiCl (50 mM). The observation that expression of PROLM24 rescued the salt sensitive phenotypes of G19 and Axt3k indicates the existence of a regulatory system that ameliorates the effect of salt stress in the transformed yeast mutants. However, the exact function of the cDNA sequence, which shows partial sequence homology to yeast UTR1 is not clear. Although UTR1 involved in ferrous uptake and iron homeostasis in yeast cells, there is no evidence to prove its role in Na+ homeostasis in yeast cells. Absence of transmembrane regions in Os06g31070 protein indicates that salt tolerance is achieved not through the direct functional complementation of the mutant genes but through an alternative mechanism.Keywords: Rice seed expression, salt stress, prolamin, salinitytolerance, Oryza sativa
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1907474 Realignment of f-actin Cytoskeleton in Osteocytes after Mechanical Loading
Authors: R. S. A. Nesbitt, J. Macione, E. Babollah, B. Adu-baffour, S. P. Kotha
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F-actin fibrils are the cytoskeleton of osteocytes. They react in a dynamic manner to mechanical loading, and strength and reposition their efforts to reinforce the cells structure. We hypothesize that f-actin is temporarly disrupted after loading and repolymerizes in a new orientation to oppose the applied load. In vitro studies are conducted to determine f-actin disruption after varying mechanical stimulus parameters that are known to affect bone formation. Results indicate that the f-actin cytoskeleton is disrupted in vitro as a function of applied mechanical stimulus parameters and that the f-actin bundles reassemble after loading induced disruption within 3 minutes after cessation of loading. The disruption of the factin cytoskeleton depends on the magnitude of stretch, the numbers of loading cycles, frequency, the insertion of rest between loading cycles and extracellular calcium. In vivo studies also demonstrate disruption of the f-actin cytoskeleton in cells embedded in the bone matrix immediately after mechanical loading. These studies suggest that adaptation of the f-actin fiber bundles of the cytoskeleton in response to applied loads occurs by disruption and subsequent repolymerization.Keywords: Mechanical loading of osteocytes, f-actin cytoskeleton, disruption, re-polymerization.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1561473 Sex Differences in Thyroid Gland Structure of Rabbits
Authors: Parchami A., Fatahian Dehkordi RF.
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The aim of the present investigation was to compare sex differences in thyroid gland structure of rabbits. Five adult male and five adult female (3.1-3.5 kg body weight) New Zealand white rabbits were used in the experiment. Results showed that at light microscopic level, there was no sex difference in microscopic appearance of the thyroid glands. At electron microscopic level, however, the mitochondria and the microvilli of the follicular cells are more numerous and the Golgi complex is also more extensive in male rabbits in comparison to females. Results obtained from micrometric measurements showed that the volume density of the follicles is higher in males than in females, but the differences are not statistically significant .The volume density of epithelium and the height of follicular cells are significantly greater in males than in females and reverse is true about the volume density of interstitium (p<0.05). The volume density of colloid is also greater in females (66±6) than in males (60±7) but the differences are not statistically significant .It was concluded that sex has limited effects on histomorphometric properties of thyroid gland in rabbits.Keywords: Rabbit, Thyroid Gland, Sex difference, Electron microscope
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2405472 Phenotypes of B Cells Differ in EBV-positive Burkitt-s lymphoma Derived Cell Lines
Authors: Irina Spaka, Rita Birkenfelde, Svetlana Kozireva, Jevgenija Osmjana, Madara Upmane, ElenaKashuba, Irina Kholodnyuk Holodnuka
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Epstein-Barr virus (EBV) is implicated in the pathogenesis of the endemic Burkitt-s lymphoma (BL). The EBVpositive BL-derived cell lines initially maintain the original tumor phenotype of EBV infection (latency I, LatI), but most of them drift toward a lymphoblast phenotype of EBV latency III (LatIII) during in vitro culturing. The aim of the present work was to characterize the B-cell subsets in EBV-positive BL cell lines and to verify whether a particular cell subset correlates with the type of EBV infection. The phenotype analysis of two EBV-negative and eleven EBV-positive (three of LatI and eight of LatIII) BL cell lines was performed by polychromatic flow cytomery, based on expression pattern of CD19, CD10, CD38, CD27, and CD5 markers. Two cell subsets, CD19+CD10+ and CD19+CD10-, were defined in LatIII BL cell lines. In both subsets, the CD27 and CD5 cell surface expression was detected in a proportion of the cells.
Keywords: B-cell subsets, Burkitt's lymphoma cell lines, EBV latency, phenotype profiles.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1958471 A Forward Automatic Censored Cell-Averaging Detector for Multiple Target Situations in Log-Normal Clutter
Authors: Musa'ed N. Almarshad, Saleh A. Alshebeili, Mourad Barkat
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A challenging problem in radar signal processing is to achieve reliable target detection in the presence of interferences. In this paper, we propose a novel algorithm for automatic censoring of radar interfering targets in log-normal clutter. The proposed algorithm, termed the forward automatic censored cell averaging detector (F-ACCAD), consists of two steps: removing the corrupted reference cells (censoring) and the actual detection. Both steps are performed dynamically by using a suitable set of ranked cells to estimate the unknown background level and set the adaptive thresholds accordingly. The F-ACCAD algorithm does not require any prior information about the clutter parameters nor does it require the number of interfering targets. The effectiveness of the F-ACCAD algorithm is assessed by computing, using Monte Carlo simulations, the probability of censoring and the probability of detection in different background environments.Keywords: CFAR, Log-normal clutter, Censoring, Probabilityof detection, Probability of false alarm, Probability of falsecensoring.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1916470 Mechanical Behaviour and Electrical Conductivity of Oxygen Separation Membrane under Uniaxial Compressive Loading
Authors: Wakako Araki, Jürgen Malzbender
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The mechanical deformation and the electrical conductivity of lanthanum strontium cobalt ferrite oxide under uniaxial compression were investigated at various temperatures up to 1073 K. The material reveals a rather complex mechanical behaviour related to its ferroelasticity and completely different stress-strain curves are obtained during the 1st and 2nd loading cycles. A distinctive ferroelastic creep was observed at 293 K whilst typical ferroelastic stress-strain curve were obtained in the temperature range from 473 K to 873 K. At 1073 K, on the other hand, high-temperature creep deformation was observed instead of ferroelastic deformation. The conductivity increases with increasing compressive stress at all the temperatures. The increase in conductivity is related to both geometrical and piezoelectric effects. From 293 K to 873 K, where the material exhibits ferroelastic behaviour, the variation in the total conductivity decreases with increasing temperature. The contribution of the piezoelectric effect to the total conductivity variation also decreases with increasing temperature and the maximum in piezoconductivity has a value of about 0.75 % at 293 K for a compressive stress of 100 MPa. There is no effect of domain switching on conductivity except for the geometric effect. At 1073 K, the conductivity is simply proportional to the compressive strain.
Keywords: Ferroelasticity, Piezoconductivity, oxygen separation membrane, perovskite.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1758469 High Temperature Oxidation of Cr-Steel Interconnects in Solid Oxide Fuel Cells
Authors: Saeed Ghali, Azza Ahmed, Taha Mattar
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Solid Oxide Fuel Cell (SOFC) is a promising solution for the energy resources leakage. Ferritic stainless steel becomes a suitable candidate for the SOFCs interconnects due to the recent advancements. Different steel alloys were designed to satisfy the needed characteristics in SOFCs interconnect as conductivity, thermal expansion and corrosion resistance. Refractory elements were used as alloying elements to satisfy the needed properties. The oxidation behaviour of the developed alloys was studied where the samples were heated for long time period at the maximum operating temperature to simulate the real working conditions. The formed scale and oxidized surface were investigated by SEM. Microstructure examination was carried out for some selected steel grades. The effect of alloying elements on the behaviour of the proposed interconnects material and the performance during the working conditions of the cells are explored and discussed. Refractory metals alloying of chromium steel seems to satisfy the needed characteristics in metallic interconnects.
Keywords: SOFCs, Cr-steel, interconnects, oxidation.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1225468 Applicability of Overhangs for Energy Saving in Existing High-Rise Housing in Different Climates
Authors: Qiong He, S. Thomas Ng
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Upgrading the thermal performance of building envelope of existing residential buildings is an effective way to reduce heat gain or heat loss. Overhang device is a common solution for building envelope improvement as it can cut down solar heat gain and thereby can reduce the energy used for space cooling in summer time. Despite that, overhang can increase the demand for indoor heating in winter due to its function of lowering the solar heat gain. Obviously, overhang has different impacts on energy use in different climatic zones which have different energy demand. To evaluate the impact of overhang device on building energy performance under different climates of China, an energy analysis model is built up in a computer-based simulation program known as DesignBuilder based on the data of a typical high-rise residential building. The energy simulation results show that single overhang is able to cut down around 5% of the energy consumption of the case building in the stand-alone situation or about 2% when the building is surrounded by other buildings in regions which predominantly rely on space cooling though it has no contribution to energy reduction in cold region. In regions with cold summer and cold winter, adding overhang over windows can cut down around 4% and 1.8% energy use with and without adjoining buildings, respectively. The results indicate that overhang might not an effective shading device to reduce the energy consumption in the mixed climate or cold regions.Keywords: Overhang, energy analysis, computer-based simulation, high-rise residential building, mutual shading, climate.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1447467 Cloning and Expression of D-Threonine Aldolase from Ensifer arboris NBRC100383
Authors: Sang-Ho Baik
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D-erythro-cyclohexylserine (D chiral unnatural β-hydroxy amino acid expected for the synthesis of drug for AIDS treatment. To develop a continuous bioconversion system with whole cell biocatalyst of D-threonine aldolase (D genes for the D-erythro-CHS production, D-threonine aldolase gene was amplified from Ensifer arboris 100383 by direct PCR amplication using two degenerated oligonucleotide primers designed based on genomic sequence of Shinorhizobium meliloti Sequence analysis of the cloned DNA fragment revealed one open-reading frame of 1059 bp and 386 amino acids. This putative D-TA gene was cloned into NdeI and EcoRI (pEnsi His-tag sequence or BamHI (pEnsi-DTA[2]) sequence of the pET21(a) vector. The expression level of the cloned gene was extremely overexpressed by E. coli BL21(DE3) transformed with pEnsi-DTA[1] compared to E. coli BL21(DE3) transformed with pEnsi-DTA[2]. When the cells expressing the wild used for D-TA enzyme activity, 12 mM glycine was successfully detected in HPLC analysis. Moreover, the whole cells harbouring the recombinant D-TA was able to synthesize D-erythro of 0.6 mg/ml in a batch reaction.Keywords: About four key words or phrases in alphabetical order, separated by commas.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1744466 Malaria Parasite Detection Using Deep Learning Methods
Authors: Kaustubh Chakradeo, Michael Delves, Sofya Titarenko
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Malaria is a serious disease which affects hundreds of millions of people around the world, each year. If not treated in time, it can be fatal. Despite recent developments in malaria diagnostics, the microscopy method to detect malaria remains the most common. Unfortunately, the accuracy of microscopic diagnostics is dependent on the skill of the microscopist and limits the throughput of malaria diagnosis. With the development of Artificial Intelligence tools and Deep Learning techniques in particular, it is possible to lower the cost, while achieving an overall higher accuracy. In this paper, we present a VGG-based model and compare it with previously developed models for identifying infected cells. Our model surpasses most previously developed models in a range of the accuracy metrics. The model has an advantage of being constructed from a relatively small number of layers. This reduces the computer resources and computational time. Moreover, we test our model on two types of datasets and argue that the currently developed deep-learning-based methods cannot efficiently distinguish between infected and contaminated cells. A more precise study of suspicious regions is required.Keywords: Malaria, deep learning, DL, convolution neural network, CNN, thin blood smears.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 655465 Synthesis and Applications of Heteronanostructured ZnO Nanowires Array
Authors: Minsu Seol, Youngjo Tak, Guenjai Kwak, Kijung Yong
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ZnO heteronanostructured nanowires arrays have been fabricated by low temperature solution method. Various heterostructures were synthesized including CdS/ZnO, CdSe/CdS/ZnO nanowires and Co3O4/ZnO, ZnO/SiC nanowires. These multifunctional heterostructure nanowires showed important applications in photocatalysts, sensors, wettability control and solar energy conversion.Keywords: ZnO nanowires, Heterostructure nanowires, solarenergy conversion, photocatalsis.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2117464 Combining ASTER Thermal Data and Spatial-Based Insolation Model for Identification of Geothermal Active Areas
Authors: Khalid Hussein, Waleed Abdalati, Pakorn Petchprayoon, Khaula Alkaabi
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In this study, we integrated ASTER thermal data with an area-based spatial insolation model to identify and delineate geothermally active areas in Yellowstone National Park (YNP). Two pairs of L1B ASTER day- and nighttime scenes were used to calculate land surface temperature. We employed the Emissivity Normalization Algorithm which separates temperature from emissivity to calculate surface temperature. We calculated the incoming solar radiation for the area covered by each of the four ASTER scenes using an insolation model and used this information to compute temperature due to solar radiation. We then identified the statistical thermal anomalies using land surface temperature and the residuals calculated from modeled temperatures and ASTER-derived surface temperatures. Areas that had temperatures or temperature residuals greater than 2σ and between 1σ and 2σ were considered ASTER-modeled thermal anomalies. The areas identified as thermal anomalies were in strong agreement with the thermal areas obtained from the YNP GIS database. Also the YNP hot springs and geysers were located within areas identified as anomalous thermal areas. The consistency between our results and known geothermally active areas indicate that thermal remote sensing data, integrated with a spatial-based insolation model, provides an effective means for identifying and locating areas of geothermal activities over large areas and rough terrain.
Keywords: Thermal remote sensing, insolation model, land surface temperature, geothermal anomalies.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1025463 A Neuro Adaptive Control Strategy for Movable Power Source of Proton Exchange Membrane Fuel Cell Using Wavelets
Authors: M. Sedighizadeh, A. Rezazadeh
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Movable power sources of proton exchange membrane fuel cells (PEMFC) are the important research done in the current fuel cells (FC) field. The PEMFC system control influences the cell performance greatly and it is a control system for industrial complex problems, due to the imprecision, uncertainty and partial truth and intrinsic nonlinear characteristics of PEMFCs. In this paper an adaptive PI control strategy using neural network adaptive Morlet wavelet for control is proposed. It is based on a single layer feed forward neural networks with hidden nodes of adaptive morlet wavelet functions controller and an infinite impulse response (IIR) recurrent structure. The IIR is combined by cascading to the network to provide double local structure resulting in improving speed of learning. The proposed method is applied to a typical 1 KW PEMFC system and the results show the proposed method has more accuracy against to MLP (Multi Layer Perceptron) method.Keywords: Adaptive Control, Morlet Wavelets, PEMFC.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1867462 Safety Study of Intravenously Administered Human Cord Blood Stem Cells in the Treatment of Symptoms Related to Chronic Inflammation
Authors: Brian M. Mehling, Louis Quartararo, Marine Manvelyan, Paul Wang, Dong-Cheng Wu
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Numerous investigations suggest that Mesenchymal Stem Cells (MSCs) in general represent a valuable tool for therapy of symptoms related to chronic inflammatory diseases. Blue Horizon Stem Cell Therapy Program is a leading provider of adult and children’s stem cell therapies. Uniquely we have safely and efficiently treated more than 600 patients with documenting each procedure. The purpose of our study is primarily to monitor the immune response in order to validate the safety of intravenous infusion of human umbilical cord blood derived MSCs (UC-MSCs), and secondly, to evaluate effects on biomarkers associated with chronic inflammation. Nine patients were treated for conditions associated with chronic inflammation and for the purpose of antiaging. They have been given one intravenous infusion of UCMSCs. Our study of blood test markers of 9 patients with chronic inflammation before and within three months after MSCs treatment demonstrates that there is no significant changes and MSCs treatment was safe for the patients. Analysis of different indicators of chronic inflammation and aging included in initial, 24-hours, two weeks and three months protocols showed that stem cell treatment was safe for the patients; there were no adverse reactions. Moreover data from follow up protocols demonstrates significant improvement in energy level, hair, nails growth and skin conditions. Intravenously administered UC-MSCs were safe and effective in the improvement of symptoms related to chronic inflammation. Further close monitoring and inclusion of more patients are necessary to fully characterize the advantages of UC-MSCs application in treatment of symptoms related to chronic inflammation.Keywords: Chronic inflammatory diseases, intravenous infusion, mesenchymal stem cells (MSCs), umbilical cord blood.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1932461 A Grid Synchronization Method Based on Adaptive Notch Filter for SPV System with Modified MPPT
Authors: Priyanka Chaudhary, M. Rizwan
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This paper presents a grid synchronization technique based on adaptive notch filter for SPV (Solar Photovoltaic) system along with MPPT (Maximum Power Point Tracking) techniques. An efficient grid synchronization technique offers proficient detection of various components of grid signal like phase and frequency. It also acts as a barrier for harmonics and other disturbances in grid signal. A reference phase signal synchronized with the grid voltage is provided by the grid synchronization technique to standardize the system with grid codes and power quality standards. Hence, grid synchronization unit plays important role for grid connected SPV systems. As the output of the PV array is fluctuating in nature with the meteorological parameters like irradiance, temperature, wind etc. In order to maintain a constant DC voltage at VSC (Voltage Source Converter) input, MPPT control is required to track the maximum power point from PV array. In this work, a variable step size P & O (Perturb and Observe) MPPT technique with DC/DC boost converter has been used at first stage of the system. This algorithm divides the dPpv/dVpv curve of PV panel into three separate zones i.e. zone 0, zone 1 and zone 2. A fine value of tracking step size is used in zone 0 while zone 1 and zone 2 requires a large value of step size in order to obtain a high tracking speed. Further, adaptive notch filter based control technique is proposed for VSC in PV generation system. Adaptive notch filter (ANF) approach is used to synchronize the interfaced PV system with grid to maintain the amplitude, phase and frequency parameters as well as power quality improvement. This technique offers the compensation of harmonics current and reactive power with both linear and nonlinear loads. To maintain constant DC link voltage a PI controller is also implemented and presented in this paper. The complete system has been designed, developed and simulated using SimPower System and Simulink toolbox of MATLAB. The performance analysis of three phase grid connected solar photovoltaic system has been carried out on the basis of various parameters like PV output power, PV voltage, PV current, DC link voltage, PCC (Point of Common Coupling) voltage, grid voltage, grid current, voltage source converter current, power supplied by the voltage source converter etc. The results obtained from the proposed system are found satisfactory.
Keywords: Solar photovoltaic systems, MPPT, voltage source converter, grid synchronization technique.
Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 1968