Search results for: enzymatic treatment
Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 1466

Search results for: enzymatic treatment

1076 Application of Statistical Approach for Optimizing CMCase Production by Bacillus tequilensis S28 Strain via Submerged Fermentation Using Wheat Bran as Carbon Source

Authors: A. Sharma, R. Tewari, S. K. Soni

Abstract:

Biofuels production has come forth as a future technology to combat the problem of depleting fossil fuels. Bio-based ethanol production from enzymatic lignocellulosic biomass degradation serves an efficient method and catching the eye of scientific community. High cost of the enzyme is the major obstacle in preventing the commercialization of this process. Thus main objective of the present study was to optimize composition of medium components for enhancing cellulase production by newly isolated strain of Bacillus tequilensis. Nineteen factors were taken into account using statistical Plackett-Burman Design. The significant variables influencing the cellulose production were further employed in statistical Response Surface Methodology using Central Composite Design for maximizing cellulase production. The optimum medium composition for cellulase production was: peptone (4.94 g/L), ammonium chloride (4.99 g/L), yeast extract (2.00 g/L), Tween-20 (0.53 g/L), calcium chloride (0.20 g/L) and cobalt chloride (0.60 g/L) with pH 7, agitation speed 150 rpm and 72 h incubation at 37oC. Analysis of variance (ANOVA) revealed high coefficient of determination (R2) of 0.99. Maximum cellulase productivity of 11.5 IU/ml was observed against the model predicted value of 13 IU/ml. This was found to be optimally active at 60oC and pH 5.5.

Keywords: Bacillus tequilensis, CMCase, Submerged Fermentation, Optimization, Plackett-Burman Design, Response Surface Methodology.

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1075 Transforming Ganges to be a Living River through Waste Water Management

Authors: P. M. Natarajan, Shambhu Kallolikar, S. Ganesh

Abstract:

By size and volume of water, Ganges River basin is the biggest among the fourteen major river basins in India. By Hindu’s faith, it is the main ‘holy river’ in this nation. But, of late, the pollution load, both domestic and industrial sources are deteriorating the surface and groundwater as well as land resources and hence the environment of the Ganges River basin is under threat. Seeing this scenario, the Indian government began to reclaim this river by two Ganges Action Plans I and II since 1986 by spending Rs. 2,747.52 crores ($457.92 million). But the result was no improvement in the water quality of the river and groundwater and environment even after almost three decades of reclamation, and hence now the New Indian Government is taking extra care to rejuvenate this river and allotted Rs. 2,037 cores ($339.50 million) in 2014 and Rs. 20,000 crores ($3,333.33 million) in 2015. The reasons for the poor water quality and stinking environment even after three decades of reclamation of the river are either no treatment/partial treatment of the sewage. Hence, now the authors are suggesting a tertiary level treatment standard of sewages of all sources and origins of the Ganges River basin and recycling the entire treated water for nondomestic uses. At 20million litres per day (MLD) capacity of each sewage treatment plant (STP), this basin needs about 2020 plants to treat the entire sewage load. Cost of the STPs is Rs. 3,43,400 million ($5,723.33 million) and the annual maintenance cost is Rs. 15,352 million ($255.87 million). The advantages of the proposed exercise are: we can produce a volume of 1,769.52 million m3 of biogas. Since biogas is energy, can be used as a fuel, for any heating purpose, such as cooking. It can also be used in a gas engine to convert the energy in the gas into electricity and heat. It is possible to generate about 3,539.04 million kilowatt electricity per annum from the biogas generated in the process of wastewater treatment in Ganges basin. The income generation from electricity works out to Rs 10,617.12million ($176.95million). This power can be used to bridge the supply and demand gap of energy in the power hungry villages where 300million people are without electricity in India even today, and to run these STPs as well. The 664.18 million tonnes of sludge generated by the treatment plants per annum can be used in agriculture as manure with suitable amendments. By arresting the pollution load the 187.42 cubic kilometer (km3) of groundwater potential of the Ganges River basin could be protected from deterioration. Since we can recycle the sewage for non-domestic purposes, about 14.75km3 of fresh water per annum can be conserved for future use. The total value of the water saving per annum is Rs.22,11,916million ($36,865.27million) and each citizen of Ganges River basin can save Rs. 4,423.83/ ($73.73) per annum and Rs. 12.12 ($0.202) per day by recycling the treated water for nondomestic uses. Further the environment of this basin could be kept clean by arresting the foul smell as well as the 3% of greenhouse gages emission from the stinking waterways and land. These are the ways to reclaim the waterways of Ganges River basin from deterioration.

Keywords: Holy Ganges River, lifeline of India, wastewater treatment and management, making Ganges permanently holy.

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1074 Treatment of Acid Mine Drainage Using Un- Activated Bentonite and Limestone

Authors: Thembelihle Nkonyane, Freeman Ntuli, Edison Muzenda

Abstract:

The use of un-activated bentonite, and un-activated bentonite blended with limestone for the treatment of acid mine drainage (AMD) was investigated. Batch experiments were conducted in a 5 L PVC reactor. Un-activated bentonite on its own did not effectively neutralize and remove heavy metals from AMD. The final pH obtained was below 4 and the metal removal efficiency was below 50% for all the metals when bentonite solid loadings of 1, 5 and 10% were used. With un-activated bentonite (1%) blended with 1% limestone, the final pH obtained was approximately 7 and metal removal efficiencies were greater than 60% for most of the metals. The Langmuir isotherm gave the best fit for the experimental data giving correlation coefficient (R2) very close to 1. Thus, it was concluded that un-activated bentonite blended with limestone is suitable for potential applications in removing heavy metals and neutralizing AMD.

Keywords: acid mine drainage, bentonite, limestone, heavy metal removal.

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1073 Investigating the Treatability of a Compost Leachate in a Hybrid Anaerobic Reactor: An Experimental Study

Authors: Shima Rajabi, Leila Vafajoo

Abstract:

Compost manufacturing plants are one of units where wastewater is produced in significantly large amounts. Wastewater produced in these plants contains high amounts of substrate (organic loads) and is classified as stringent waste which creates significant pollution when discharged into the environment without treatment. A compost production plant in the one of the Iran-s province treating 200 tons/day of waste is one of the most important environmental pollutant operations in this zone. The main objectives of this paper are to investigate the compost wastewater treatability in hybrid anaerobic reactors with an upflow-downflow arrangement, to determine the kinetic constants, and eventually to obtain an appropriate mathematical model. After starting the hybrid anaerobic reactor of the compost production plant, the average COD removal rate efficiency was 95%.

Keywords: Leachate treatment, anaerobic hybrid reactor

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1072 Effects of Oilfield Water Treated by Electroflocculation and Reverse Osmosis in a Typical Brazilian Semiarid Soil

Authors: P. S. A. Souza, M. R. C. Marques, M. M. Rigo, A. A. Cerqueira, J. L. Paiva, F. Merçon, D. V. Perez

Abstract:

Produced water (PW), which is water extracted along with oil, is the largest waste stream in the oil and gas industry. With the proper treatment, this wastewater can be used in agricultural irrigation. This study evaluated the effects the application of PW treated by electroflocculation (EF) and combined electroflocculation-reverse osmosis (EF-RO) on soil salinity and sodification parameters. Excessive sodium levels in PW treated by EF may affect soil structural stability and plant growth, and tends to accumulate in upper layers, displacing the nutrient K to deeper layers of the soil profile. PW treated by EF-RO did not promote salinization and soil sodification, indicating that this combined technique may be a viable alternative for oily water treatment aiming at irrigation use in semiarid regions.

Keywords: Electroflocculation, irrigation, produced water, reverse osmosis, soil.

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1071 Literature-Based Discoveries in Lupus Treatment

Authors: Oluwaseyi Jaiyeoba, Vetria Byrd

Abstract:

Systemic lupus erythematosus (aka lupus) is a chronic disease known for its chameleon-like ability to mimic symptoms of other diseases rendering it hard to detect, diagnose and treat. The heterogeneous nature of the disease generates disparate data that are often multifaceted and multi-dimensional. Musculoskeletal manifestation of lupus is one of the most common clinical manifestations of lupus. This research links disparate literature on the treatment of lupus as it affects the musculoskeletal system using the discoveries from literature-based research articles available on the PubMed database. Several Natural Language Processing (NPL) tools exist to connect disjointed but related literature, such as Connected Papers, Bitola, and Gopalakrishnan. Literature-based discovery (LBD) has been used to bridge unconnected disciplines based on text mining procedures. The technical/medical literature consists of many technical/medical concepts, each having its  sub-literature. This approach has been used to link Parkinson’s, Raynaud, and Multiple Sclerosis treatment within works of literature.  Literature-based discovery methods can connect two or more related but disjointed literature concepts to produce a novel and plausible approach to solving a research problem. Data visualization techniques with the help of natural language processing tools are used to visually represent the result of literature-based discoveries. Literature search results can be voluminous, but Data visualization processes can provide insight and detect subtle patterns in large data. These insights and patterns can lead to discoveries that would have otherwise been hidden from disjointed literature. In this research, literature data are mined and combined with visualization techniques for heterogeneous data to discover viable treatments reported in the literature for lupus expression in the musculoskeletal system. This research answers the question of using literature-based discovery to identify potential treatments for a multifaceted disease like lupus. A three-pronged methodology is used in this research: text mining, natural language processing, and data visualization. These three research-related fields are employed to identify patterns in lupus-related data that, when visually represented, could aid research in the treatment of lupus. This work introduces a method for visually representing interconnections of various lupus-related literature. The methodology outlined in this work is the first step toward literature-based research and treatment planning for the musculoskeletal manifestation of lupus. The results also outline the interconnection of complex, disparate data associated with the manifestation of lupus in the musculoskeletal system. The societal impact of this work is broad. Advances in this work will improve the quality of life for millions of persons in the workforce currently diagnosed and silently living with a musculoskeletal disease associated with lupus.

Keywords: Systemic lupus erythematosus, LBD, Data Visualization, musculoskeletal system, treatment.

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1070 Correction of Frequent English Writing Errors by Using Coded Indirect Corrective Feedback and Error Treatment: The Case of Reading and Writing English for Academic Purposes II

Authors: Chaiwat Tantarangsee

Abstract:

The purposes of this study are 1) to study the frequent English writing errors of students registering the course: Reading and Writing English for Academic Purposes II, and 2) to find out the results of writing error correction by using coded indirect corrective feedback and writing error treatments. Samples include 28 2nd year English Major students, Faculty of Education, Suan Sunandha Rajabhat University. Tool for experimental study includes the lesson plan of the course; Reading and Writing English for Academic Purposes II, and tool for data collection includes 4 writing tests of short texts. The research findings disclose that frequent English writing errors found in this course comprise 7 types of grammatical errors, namely Fragment sentence, Subject-verb agreement, Wrong form of verb tense, Singular or plural noun endings, Run-ons sentence, Wrong form of verb pattern and Lack of parallel structure. Moreover, it is found that the results of writing error correction by using coded indirect corrective feedback and error treatment reveal the overall reduction of the frequent English writing errors and the increase of students’ achievement in the writing of short texts with the significance at .05.

Keywords: Coded indirect corrective feedback, error correction, and error treatment.

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1069 Mechanical Properties of Enset Fibers Obtained from Different Breeds of Enset Plant

Authors: Diriba T. Balcha, Boris Kulig, Oliver Hensel, Eyassu Woldesenbet

Abstract:

Enset fiber is agricultural waste and available in a surplus amount in Ethiopia. However, the hypothesized variation in properties of this fiber due to diversity of its plant source breed, fiber position within plant stem and chemical treatment duration had not proven that its application for the development of composite products is problematic. Currently, limited data are known on the functional properties of the fiber as a potential functional fiber. Thus, an effort is made in this study to narrow the knowledge gaps by characterizing it. The experimental design was conducted using Design-Expert software and the tensile test was conducted on Enset fiber from 10 breeds: Dego, Dirbo, Gishera, Itine, Siskela, Neciho, Yesherkinke, Tuzuma, Ankogena, and Kucharkia. The effects of 5% Na-OH surface treatment duration and fiber location along and across the plant pseudostem was also investigated. The test result shows that the rupture stress variation is not significant among the fibers from 10 Enset breeds. However, strain variation is significant among the fibers from 10 Enset breeds that breed Dego fiber has the highest strain before failure. Surface treated fibers showed improved rupture strength and elastic modulus per 24 hours of treatment duration. Also, the result showed that chemical treatment can deteriorate the load-bearing capacity of the fiber. The raw fiber has the higher load-bearing capacity than the treated fiber. And, it was noted that both the rupture stress and strain increase in the top to bottom gradient, whereas there is no significant variation across the stem. Elastic modulus variation both along and across the stem was insignificant. The rupture stress, elastic modulus, and strain result of Enset fiber are 360.11 ± 181.86 MPa, 12.80 ± 6.85 GPa and 0.04 ± 0.02 mm/mm, respectively. These results show that Enset fiber is comparable to other natural fibers such as abaca, banana, and sisal fibers and can be used as alternatives natural fiber for composites application. Besides, the insignificant variation of properties among breeds and across stem is essential for all breeds and all leaf sheath of the Enset fiber plant for fiber extraction. The use of short natural fiber over the long is preferable to reduce the significant variation of properties along the stem or fiber direction. In conclusion, Enset fiber application for composite product design and development is mechanically feasible.

Keywords: Agricultural waste, chemical treatment, fiber characteristics, natural fiber.

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1068 Scope and Application of Collaborative Tools and Digital Manufacturing in Dentistry

Authors: S. Mohan Kumar, Rajashekar Patil, Tanuja Ajit Desphande

Abstract:

It is necessary to incorporate technological advances achieved in the field of engineering into dentistry in order to enhance the process of diagnosis, treatment planning and enable the doctors to render better treatment to their patients. To achieve this ultimate goal long distance collaborations are often necessary. This paper discusses the various collaborative tools and their applications to solve a few burning problems confronted by the dentists. Customization is often the solution to most of the problems. But rapid designing, development and cost effective manufacturing is a difficult task to achieve. This problem can be solved using the technique of digital manufacturing. Cases from 6 major branches of dentistry have been discussed and possible solutions with the help of state of art technology using rapid digital manufacturing have been proposed in the present paper. The paper also entails the usage of existing tools in collaborative and digital manufacturing area.

Keywords: Customisation, collaborative tools, dentistry, digital manufacturing.

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1067 Thiosulfate Leaching of the Auriferous Ore from Castromil Deposit: A Case Study

Authors: Rui Sousa, Aurora Futuro, António Fiúza

Abstract:

The exploitation of gold ore deposits is highly dependent on efficient mineral processing methods, although actual perspectives based on life-cycle assessment introduce difficulties that were unforeseen in a very recent past. Cyanidation is the most applied gold processing method, but the potential environmental problems derived from the usage of cyanide as leaching reagent led to a demand for alternative methods. Ammoniacal thiosulfate leaching is one of the most important alternatives to cyanidation. In this article, some experimental studies carried out in order to assess the feasibility of thiosulfate as a leaching agent for the ore from the unexploited Portuguese gold mine of Castromil. It became clear that the process depends on the concentrations of ammonia, thiosulfate and copper. Based on this fact, a few leaching tests were performed in order to assess the best reagent prescription, and also the effects of different combination of these concentrations. Higher thiosulfate concentrations cause the decrease of gold dissolution. Lower concentrations of ammonia require higher thiosulfate concentrations, and higher ammonia concentrations require lower thiosulfate concentrations. The addition of copper increases the gold dissolution ratio. Subsequently, some alternative operatory conditions were tested such as variations in temperature and in the solid/liquid ratio as well as the application of a pre-treatment before the leaching stage. Finally, thiosulfate leaching was compared to cyanidation. Thiosulfate leaching showed to be an important alternative, although a pre-treatment is required to increase the yield of the gold dissolution.

Keywords: Gold, leaching, pre-treatment, thiosulfate.

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1066 Evaluation of Superabsorbent Application on Corn Yield under Deficit Irrigation

Authors: D. Khodadadi Dehkordi

Abstract:

This research was planned in order to study the effect of drought stress and different levels of Superabsorbent and their effect on grain yield, biologic yield and harvest index. In this study, 3 different depths of irrigation were considered as the main treatment I1, I2, I3 as 100, 75 and 50 percent of water requirement of plants respectively and different levels of Superabsorbent were used as secondary treatment (S0, S1, S2 and S3, equal to 0 (control), 15, 30 and 45 gr/m2 respectively). According to the results, independent effects of irrigation and Superabsorbent treatments at 1% level on biologic and grain yield of corn were significant. In addition, independent effect of irrigation treatments at 5% level on harvest index was significant. But independent effect of Superabsorbent treatments on harvest index was not significant.

Keywords: Corn, Deficit irrigation, Superabsorbent, Yield.

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1065 Integrated Wastewater Reuse Project of the Faculty of Sciences Ain Chock, Morocco

Authors: Nihad Chakri, Btissam El Amrani, Faouzi Berrada, Fouad Amraoui

Abstract:

In Morocco, water scarcity requires the exploitation of non-conventional resources. Rural areas are under-equipped with sanitation infrastructure, unlike urban areas. Decentralized and low-cost solutions could improve the quality of life of the population and the environment. In this context, the Faculty of Sciences Ain Chock (FSAC) has undertaken an integrated project to treat part of its wastewater using a decentralized compact system. The project will propose alternative solutions that are inexpensive and adapted to the context of peri-urban and rural areas in order to treat the wastewater generated and to use it for irrigation, watering and cleaning. For this purpose, several tests were carried out in the laboratory in order to develop a liquid waste treatment system optimized for local conditions. Based on the results obtained at laboratory scale of the different proposed scenarios, we designed and implemented a prototype of a mini wastewater treatment plant for the faculty. In this article, we will outline the steps of dimensioning, construction and monitoring of the mini-station in our faculty.

Keywords: Wastewater, purification, response methodology surfaces optimization, vertical filter, Moving Bed Biofilm Reactors, MBBR process, sizing, prototype, Faculty of Sciences Ain Chock, decentralized approach, mini wastewater treatment plant, reuse of treated wastewater reuse, irrigation, sustainable development.

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1064 Application of Moringa oleifera Seed in Removing Colloids from Turbid Wastewater

Authors: H. Zemmouri, H. Lounic, N. Mameri

Abstract:

The present study aims to investigate the performance of Moringa oleifera seed extract as natural coagulant in clarification of secondary wastewater treatment plant (MWWTP) located in East of Algiers, Algeria. Coagulation flocculation performance of Moringa oleifera was evaluated through supernatant residual turbidity after jar test trials. Various influence parameters namely Moringa oleifera dosage and pH have been considered. Tests on Reghaia wastewater, having 129 NTU of initial turbidity, showed a removal of 69.45% of residual turbidity with only 1.5 mg/l of Moringa oleifera. This sufficient removal capability encourages the use of this bioflocculant for treatment of turbid waters. Indeed, Moringa oleifera which is a natural resource available locally (South of Algeria) coupled to the non-toxicity, biocompatibility and biodegradability, may be a very interesting alternative to the conventional coagulants used so far.

Keywords: Coagulation flocculation, colloids, Moringa oleifera, secondary wastewater.

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1063 Vibrational Behavior of Cylindrical Shells in Axial Magnetic Field

Authors: Sedrak Vardanyan

Abstract:

The investigation of the vibrational character of magnetic cylindrical shells placed in an axial magnetic field has important practical applications. In this work, we study the vibrational behaviour of such a cylindrical shell by making use of the so-called exact space treatment, which does not assume any hypothesis. We discuss the effects of several practically important boundary conditions on the vibrations of the described setup. We find that, for some cases of boundary conditions, e.g. clamped, simply supported or peripherally earthed, as well as for some values of the wave numbers, the vibrational frequencies of the shell are approximately zero. The theoretical and numerical exploration of this fact confirms that the vibrations are absent or attenuate very rapidly. For all the considered cases, the imaginary part of the frequencies is negative, which implies stability for the vibrational process.

Keywords: Free vibrations, magnetic cylindrical shells, exact space treatment, bending vibrational frequencies.

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1062 Formation of Byproducts during Regeneration of Various Graphitic Adsorbents in a Batch Electrochemical Reactor

Authors: S. N. Hussain, H. M. A. Asghar, H. Sattar, N. W. Brown, E. P. L. Roberts

Abstract:

A water treatment technology employing the adsorption of dissolved organic contaminants from water and their electrochemical regeneration has been commercialized by Arvia Technology Ltd, UK. This technology focuses the adsorption of pollutants onto the surface of low surface area graphite based adsorbents followed by the anodic oxidation of adsorbed species in an electrochemical cell. However, some of the adsorbed species may lead to the formation of intermediate breakdown products due to incomplete oxidation. The information regarding the formation of breakdown products during electrochemical regeneration of these adsorbents is important for the effective application of this process to water treatment. In the present paper, the formation of the break down products during electrochemical regeneration of various graphite based adsorbents has been demonstrated.

Keywords: Arvia®, Adsorption, Electrochemical Regeneration, Breakdown products.

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1061 Optimization of Air Pollution Control Model for Mining

Authors: Zunaira Asif, Zhi Chen

Abstract:

The sustainable measures on air quality management are recognized as one of the most serious environmental concerns in the mining region. The mining operations emit various types of pollutants which have significant impacts on the environment. This study presents a stochastic control strategy by developing the air pollution control model to achieve a cost-effective solution. The optimization method is formulated to predict the cost of treatment using linear programming with an objective function and multi-constraints. The constraints mainly focus on two factors which are: production of metal should not exceed the available resources, and air quality should meet the standard criteria of the pollutant. The applicability of this model is explored through a case study of an open pit metal mine, Utah, USA. This method simultaneously uses meteorological data as a dispersion transfer function to support the practical local conditions. The probabilistic analysis and the uncertainties in the meteorological conditions are accomplished by Monte Carlo simulation. Reasonable results have been obtained to select the optimized treatment technology for PM2.5, PM10, NOx, and SO2. Additional comparison analysis shows that baghouse is the least cost option as compared to electrostatic precipitator and wet scrubbers for particulate matter, whereas non-selective catalytical reduction and dry-flue gas desulfurization are suitable for NOx and SO2 reduction respectively. Thus, this model can aid planners to reduce these pollutants at a marginal cost by suggesting control pollution devices, while accounting for dynamic meteorological conditions and mining activities.

Keywords: Air pollution, linear programming, mining, optimization, treatment technologies.

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1060 Higher Plants Ability to Assimilate Explosives

Authors: G. Khatisashvili, M. Gordeziani, G. Adamia, E. Kvesitadze, T. Sadunishvili, G. Kvesitadze

Abstract:

The ability of agricultural and decorative plants to absorb and detoxify TNT and RDX has been studied. All tested 8 plants, grown hydroponically, were able to absorb these explosives from water solutions: Alfalfa > Soybean > Chickpea> Chikling vetch >Ryegrass > Mung bean> China bean > Maize. Differently from TNT, RDX did not exhibit negative influence on seed germination and plant growth. Moreover, some plants, exposed to RDX containing solution were increased in their biomass by 20%. Study of the fate of absorbed [1-14ðí]-TNT revealed the label distribution in low and high-molecular mass compounds, both in roots and above ground parts of plants, prevailing in the later. Content of 14ðí in lowmolecular compounds in plant roots are much higher than in above ground parts. On the contrary, high-molecular compounds are more intensively labeled in aboveground parts of soybean. Most part (up to 70%) of metabolites of TNT, formed either by enzymatic reduction or oxidation, is found in high molecular insoluble conjugates. Activation of enzymes, responsible for reduction, oxidation and conjugation of TNT, such as nitroreductase, peroxidase, phenoloxidase and glutathione S-transferase has been demonstrated. Among these enzymes, only nitroreductase was shown to be induced in alfalfa, exposed to RDX. The increase in malate dehydrogenase activities in plants, exposed to both explosives, indicates intensification of Tricarboxylic Acid Cycle, that generates reduced equivalents of NAD(P)H, necessary for functioning of the nitroreductase. The hypothetic scheme of TNT metabolism in plants is proposed.

Keywords: Higher plants, TNT, RDX, transformation.

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1059 Construction of a Fusion Gene Carrying E10A and K5 with 2A Peptide-Linked by Using Overlap Extension PCR

Authors: Tiancheng Lan

Abstract:

E10A is a kind of replication-defective adenovirus which carries the human endostatin gene to inhibit the growth of tumors. Kringle 5(K5) has almost the same function as angiostatin to also inhibit the growth of tumors since they are all the byproduct of the proteolytic cleavage of plasminogen. Tumor size increasing can be suppressed because both of the endostatin and K5 can restrain the angiogenesis process. Therefore, in order to improve the treatment effect on tumor, 2A peptide is used to construct a fusion gene carrying both E10A and K5. Using 2A peptide is an ideal strategy when a fusion gene is expressed because it can avoid many problems during the expression of more than one kind of protein. The overlap extension PCR is also used to connect 2A peptide with E10A and K5. The final construction of fusion gene E10A-2A-K5 can provide a possible new method of the anti-angiogenesis treatment with a better expression performance.

Keywords: E10A, Kringle 5, 2A peptide, overlap extension PCR.

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1058 Removal of Hexavalent Chromium from Wastewater by Use of Scrap Iron

Authors: Marius Gheju, Rodica Pode

Abstract:

Hexavalent chromium is highly toxic to most living organisms and a known human carcinogen by the inhalation route of exposure. Therefore, treatment of Cr(VI) contaminated wastewater is essential before their discharge to the natural water bodies. Cr(VI) reduction to Cr(III) can be beneficial because a more mobile and more toxic chromium species is converted to a less mobile and less toxic form. Zero-valence-state metals, such as scrap iron, can serve as electron donors for reducing Cr(VI) to Cr(III). The influence of pH on scrap iron capacity to reduce Cr(VI) was investigated in this study. Maximum reduction capacity of scrap iron was observed at the beginning of the column experiments; the lower the pH, the greater the experiment duration with maximum scrap iron reduction capacity. The experimental results showed that highest maximum reduction capacity of scrap iron was 12.5 mg Cr(VI)/g scrap iron, at pH 2.0, and decreased with increasing pH up to 1.9 mg Cr(VI)/g scrap iron at pH = 7.3.

Keywords: hexavalent chromium, heavy metals, scrap iron, reduction capacity, wastewater treatment.

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1057 Inorganic Anion Removal from Water Using Natural Adsorbents

Authors: A. Ortuzar, I. Escondrillas, F. Mijangos

Abstract:

There is a need for new systems that can be attached to drinking water treatment plants and have the required treatment capacity as well as the selectivity regarding components derived from anthropogenic activities. In a context of high volumes of water and low concentration of contaminants, adsorption/interchange processes are appealing since they meet the required features. Iron oxides such as siderite and molysite, which are respectively based on FeCO3 and FeCl3, can be found in nature. In this work, their observed performance, raw or roasted at different temperatures, as adsorbents of some inorganic anions is discussed. Roasted 1:1 FeCO3: FeCl3 mixture was very selective for arsenic and allowed a 100% removal of As from a 10 mg L-1 As solution. Besides, the 1:1 FeCO3 and FeCl3 mixture roasted at 500 ºC showed good selectivity for, in order of preference, arsenate, bromate, phosphate, fluoride and nitrate anions with distribution coefficients of, respectively, 4200, 2800, 2500 0.4 and 0.03 L g-1.

Keywords: Drinking water, natural adsorbent materials, removal, selectivity.

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1056 High-Intensity Nanosecond Pulsed Electric Field effects on Early Physiological Development in Arabidopsis thaliana

Authors: Wisuwat Songnuan, Phumin Kirawanich

Abstract:

The influences of pulsed electric fields on early physiological development in Arabidopsis thaliana were studied. Inside a 4-mm electroporation cuvette, pre-germination seeds were subjected to high-intensity, nanosecond electrical pulses generated using laboratory-assembled pulsed electric field system. The field strength was varied from 5 to 20 kV.cm-1 and the pulse width and the pulse number were maintained at 10 ns and 100, respectively, corresponding to the specific treatment energy from 300 J.kg-1 to 4.5 kJ.kg-1. Statistical analyses on the average leaf area 5 and 15 days following pulsed electric field treatment showed that the effects appear significant the second week after treatments with a maximum increase of 80% compared to the control (P < 0.01).

Keywords: Arabidopsis thaliana, full-wave analysis, leaf area, high-intensity nanosecond pulsed electric fields

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1055 Influence of Garbage Leachate on Soil Reaction,Salinity and Soil Organic Matter in East of Isfahan

Authors: Ebrahim Panahpour, Ali Gholami, Amir Hossein Davami

Abstract:

During this day a considerable amount of Leachate is produced with high amounts of organic material and nutrients needed plants. This study has done in order to scrutinize the effect of Leachate compost on the pH, EC and organic matter percentage in the form of statistical Factorial plan through randomizing block design with three main and two minor treatments and also three replications during three six month periods. Major treatments include N: Irrigation with the region-s well water as a control, I: Frequent irrigation with well water and Leachate, C: Mixing Leachate and water well (25 percent leachate + 75 percent ordinary well water) and secondary treatments, include DI: surface drip irrigation and SDI: sub surface drip irrigation. Results of this study indicated significant differences between treatments and also there were mixing up with the control treatment in the reduction of pH, increasing soluble salts and also increasing the organic matter percentage. This increase is proportional to the amount of added Leachate and in the treatment also proportional to higher mixture of frequent treatment. Therefore, since creating an acidic pH increases the ability to absorb some nutrient elements such as phosphorus, iron, zinc, copper and manganese are increased and the other hand, organic materials also improve many physical and chemical properties of soil are used in Leachate trash Consider health issues as refined in the green belts around cities as a liquid fertilizer recommended.

Keywords: Leachate, compost, drip irrigation, liquid fertilizer, soil reaction.

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1054 Analysis of Fertilizer Effect in the Tilapia Growth of Mozambique (Oreochromis mossambicus)

Authors: Sérgio Afonso Mulema, Andrés Carrión García, Vicente Ernesto

Abstract:

This paper analyses the effect of fertilizer (organic and inorganic) in the growth of tilapia. An experiment was implemented in the Aquapesca Company of Mozambique; there were considered four different treatments. Each type of fertilizer was applied in two of these treatments; a feed was supplied to the third treatment, and the fourth was taken as control. The weight and length of the tilapia were used as the growth parameters, and to measure the water quality, the physical-chemical parameters were registered. The results show that the weight and length were different for tilapias cultivated in different treatments. These differences were evidenced mainly by organic and feed treatments, where there was the largest and smallest value of these parameters, respectively. In order to prove that these differences were caused only by applied treatment without interference for the aquatic environment, a Fisher discriminant analysis was applied, which confirmed that the treatments were exposed to the same environment condition.

Keywords: Fertilizer, tilapia, growth, statistical methods.

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1053 Effect of Filler Metal Diameter on Weld Joint of Carbon Steel SA516 Gr 70 and Filler Metal SFA 5.17 in Submerged Arc Welding SAW

Authors: A. Nait Salah, M. Kaddami

Abstract:

This work describes an investigation on the effect of filler metals diameter to weld joint, and low alloy carbon steel A516 Grade 70 is the base metal. Commercially SA516 Grade70 is frequently used for the manufacturing of pressure vessels, boilers and storage tank, etc. In fabrication industry, the hardness of the weld joint is between the important parameters to check, after heat treatment of the weld. Submerged arc welding (SAW) is used with two filler metal diameters, and this solid wire electrode is used for SAW non-alloy and for fine grain steels (SFA 5.17). The different diameters were selected (Ø = 2.4 mm and Ø = 4 mm) to weld two specimens. Both specimens were subjected to the same preparation conditions, heat treatment, macrograph, metallurgy micrograph, and micro-hardness test. Samples show almost similar structure with highest hardness. It is important to indicate that the thickness used in the base metal is 22 mm, and all specifications, preparation and controls were according to the ASME section IX. It was observed that two different filler metal diameters performed on two similar specimens demonstrated that the mechanical property (hardness) increases with decreasing diameter. It means that even the heat treatment has the same effect with the same conditions, the filler metal diameter insures a depth weld penetration and better homogenization. Hence, the SAW welding technique mentioned in the present study is favorable to implicate for the industry using the small filler metal diameter.

Keywords: ASME, base metal, filler metal, micro-hardness test, submerged arc welding.

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1052 Seed Treatment during Germination in Linseed to Overcome Salt and Drought Stresses (Linum usitatissimum L.)

Authors: Kadkhodaie A., Bagheri M.

Abstract:

Evaluation of crop plants resistance to environmental stresses specially in germination stage is a critical factor in their selection in different conditions of cultivation. Therefore use of a procedure in controllable situation can help to evaluate plants reaction to stress quickly and precisely. In order to study germination characteristics of flax in water and salinity stress conditions were conducted two laboratories experimental. The two experimental were conducted in 4-replicant completing random design for salinity and water stress. The treatment, for salinity and water stress was three potential (zero, 40, 80 mM) of NaCl and three potential (zero, -2, -4 bar) of PEG respectively. Germination percentage and rate, in addition to Radical and plumule length and dry-weight and plumule/Radical ration were measured. All of characteristics reduce under water stress conditions. salinity stress significant reduce germination rate and Radical and plumule length of flax seeds. Hydropriming and osmopriming significant increased germination rate, plumule length and plumule/Radical ration ration of flax seeds. But germination percentage and Radical and plumule dry weight significant increased only in hydropriming treat. Hydropriming and osmopriming could not be used to improved germination under saline and drought stress. But has more tolerance in salinity and drought stress in flax by less reduce in Radical and plumule length under saline and drought stress.

Keywords: linseed, salt stress, water stress, seed treatment, Germination

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1051 Treatment of Biowaste (Generated in Biodiesel Process) - A New Strategy for Green Environment and Horticulture Crop

Authors: Shivani Chaturvedi, Santosh Satya, S. K. Tiwari

Abstract:

Recent research on seeds of bio-diesel plants like Jatropha curcas, constituting 40-50% bio-crude oil indicates its potential as one of the most promising alternatives to conventional sources of energy. Also, limited studies on utilization of de-oiled cake have revealed that Jatropha bio-waste has good potential to be used as organic fertilizers produced via aerobic and anaerobic treatment. However, their commercial exploitation has not yet been possible. The present study aims at developing appropriate bio-processes and formulations utilizing Jatropha seed cake as organic fertilizer, for improving the growth of Polianthes tuberose L. (Tuberose). Pot experiments were carried out by growing tuberose plants on soil treated with composted formulations of Jatropha de-oiled cake, Farm Yard Manure (FYM) and inorganic fertilizers were also blended in soil. The treatment was carried out through soil amendment as well as foliar spray. The growth and morphological parameters were monitored for entire crop cycle. The growth Length and number of leaves, spike length, rachis length, number of bulb per plant and earliness of sprouting of bulb and yield enhancement were comparable to that achieved under inorganic fertilizer. Furthermore, performance of inorganic fertilizer also showed an improvement when blended with composted bio-waste. These findings would open new avenues for Jatropha based bio-wastes to be composted and used as organic fertilizers for commercial floriculture.

Keywords: Organic fertilizer, Jaropha cake, Tuberose (Polianthes tuberosa L.).

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1050 Refining Waste Spent Hydroprocessing Catalyst and Their Metal Recovery

Authors: Meena Marafi, Mohan S. Rana

Abstract:

Catalysts play an important role in producing valuable fuel products in petroleum refining; but, due to feedstock’s impurities catalyst gets deactivated with carbon and metal deposition. The disposal of spent catalyst falls under the category of hazardous industrial waste that requires strict agreement with environmental regulations. The spent hydroprocessing catalyst contains Mo, V and Ni at high concentrations that have been found to be economically significant for recovery. Metal recovery process includes deoiling, decoking, grinding, dissolving and treatment with complexing leaching agent such as ethylene diamine tetra acetic acid (EDTA). The process conditions have been optimized as a function of time, temperature and EDTA concentration in presence of ultrasonic agitation. The results indicated that optimum condition established through this approach could recover 97%, 94% and 95% of the extracted Mo, V and Ni, respectively, while 95% EDTA was recovered after acid treatment.

Keywords: Spent catalyst, deactivation, hydrotreating, spent catalyst.

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1049 Mechanical Properties of the Palm Fibers Reinforced HDPE Composites

Authors: Daniella R. Mulinari, Araujo J. F. Marina, Gabriella S. Lopes

Abstract:

Natural fibers are used in polymer composites to improve mechanical properties to replace inorganic reinforcing agents produced by non-renewable resources. The present study investigates the tensile and flexural behaviors of palm fibers-high density polyethylene (HDPE) composite as a function of volume fraction. The surface of the fibers was treated by mercerization treatments to improve the wetting behavior of the apolar HDPE. The treatment characterization was obtained by scanning electron microscopy, X-Ray diffraction and infrared spectroscopy. Results evidences that a good adhesion interfacial between fibers-matrix caused an increase strength and modulus flexural as well as tensile strength in the modified fibers/HDPE composites when compared to the pure HDPE and untreated fibers reinforced composites.

Keywords: Mechanical properties, palm fibers, polymer composites, surface treatment.

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1048 Investigation on Fluid Flow Characteristics of the Orifice in Nuclear Power Plant

Authors: Nam-Seok Kim, Sang-Kyu Lee, Byung-Soo Shin, O-Hyun Keum

Abstract:

The present paper represents a methodology for investigating flow characteristics near orifice plate by using a commercial computational fluid dynamics code. The flow characteristics near orifice plate which is located in the auxiliary feedwater system were modeled via three different levels of grid and four different types of Reynolds Averaged Navier-Stokes (RANS) equations with proper near-wall treatment. The results from CFD code were compared with experimental data in terms of differential pressure through the orifice plate. In this preliminary study, the Realizable k-ε and the Reynolds stress models with enhanced wall treatment were suitable to analyze flow characteristics near orifice plate, and the results had a good agreement with experimental data.

Keywords: Auxiliary Feedwater, Computational Fluid Dynamics, Orifice, Nuclear Power Plant

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1047 Development of Composite Adsorbent for Waste Water Treatment Using Adsorption and Electrochemical Regeneration

Authors: H. M. A. Asghar, S. N. Hussain, E. P. L. Roberts, N. W. Brown, H. Sattar

Abstract:

A unique combination of adsorption and electrochemical regeneration with a proprietary adsorbent material called Nyex 100 was introduced at the University of Manchester for waste water treatment applications. Nyex 100 is based on graphite intercalation compound. It is non porous and electrically conducing adsorbent material. This material exhibited very small BET surface area i.e. 2.75 m2g-1, in consequence, small adsorptive capacities for the adsorption of various organic pollutants were obtained. This work aims to develop composite adsorbent material essentially capable of electrochemical regeneration coupled with improved adsorption characteristics. An organic dye, acid violet 17 was used as standard organic pollutant. The developed composite material was successfully electrochemically regenerated using a DC current of 1 A for 60 minutes. Regeneration efficiency was maintained at around 100% for five adsorption-regeneration cycles.

Keywords: Adsorption, electrically conducting adsorbent material, electrochemical regeneration, waste water.

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