Yuji Ito

Publications

1 Sewer Culvert Installation Method to Accommodate Underground Construction in an Urban Area with Narrow Streets (The Development of Shield Switching Type Micro-Tunneling Method and the Introduction of Construction Examples)

Authors: Osamu Igawa, Hiroshi Kouchiwa, Yuji Ito

Abstract:

In recent years, a reconstruction project for sewer  pipelines has been progressing in Japan with the aim of renewing old  sewer culverts. However, it is difficult to secure a sufficient base area  for shafts in an urban area because many streets are narrow with a  complex layout. As a result, construction in such urban areas is  generally very demanding.  In urban areas, there is a strong requirement for a safe, reliable and  economical construction method that does not disturb the public’s  daily life and urban activities. With this in mind, we developed a new  construction method called the “shield switching type micro-tunneling  method,” which integrates the micro-tunneling method and shield  method.  In this method, pipeline is constructed first for sections that are  gently curved or straight using the economical micro-tunneling  method, and then the method is switched to the shield method for  sections with a sharp curve or a series of curves without establishing  an intermediate shaft.  This paper provides the information, features and construction  examples of this newly developed method.

 

Keywords: micro-tunneling method, sharp curve, shield method, switching type, Secondary lining applied RC segment

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Abstracts

3 Characterization of Monoclonal Antibodies Specific for Synthetic Cannabinoids

Authors: Hiroshi Nakayama, Yuji Ito

Abstract:

Synthetic cannabinoids have attracted much public attention recently in Japan. 1-pentyl-3-(1-naphthoyl)-indole (JWH-018), 1-pentyl-2-methyl-3-(1-naphthoyl) indole (JWH-015), 1-(5-fluoropentyl)-3- (1-(2,2,3,3- tetramethylcyclopropyl)) indole (XLR-11) and 1-methyl-3- (1-admantyl) indole (JWH-018 adamantyl analog) are known as synthetic cannabinoids and are also considered dangerous illegal drugs in Japan. It has become necessary to develop sensitive and useful methods for detection of synthetic cannabinoids. We produced two monoclonal antibodies (MAb) against synthetic cannabinoids, named NT1 (IgG1) and NT2 (IgG1), using Hybridoma technology. The cross-reactivity of these produced MAbs was evaluated using a competitive enzyme-linked immunosorbent assay (ELISA). In the results, we found both of these antibodies recognize many kinds of synthetic cannabinoids analog. However, neither of these antibodies recognizes naphtoic acid, 1-methyl-indole and indole known as a raw material of synthetic cannabinoid. Thus, the MAbs produced in this study could be a useful tool for the detection of synthetic cannabinoids.

Keywords: Sensor, Monoclonal Antibody, ELISA, synthetic cannabinoid

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2 Introduction of PMMA-Tag to VHH for Improving Recovery and Immobilization Rate of VHHS

Authors: Bongmun Kang, Kagnari Yamakawa, Yoshihisa Hagihara, Yuji Ito, Michimasa Kishimoto, Yoichi Kumada

Abstract:

The PMMA-tag was genetically fused with the C-terminal region of VHH molecules. This antibody, VHH, is known as a single-chain domain, which is devoid of light chains. The PMMA-tag, which could affect the isoelectric point (pI) changeable with a charge of amino acid in VHHs were closely related to the solubility of VHH molecules during refolding. The genetic fusion of PMMA-tag to C-terminal region of VHHs significantly affects the recovery of their soluble protein during refolding by 50 mM TAPS at pH 8.5. It could be refolded with a recovery of more than 95% by dialysis at pH 8.5. A marked difference in the antigen-binding activities in the adsorption state was significantly high in VHH-PM compared to the wild type of VHH. There are approximately 8-fold differences in the antigen-binding activities in the adsorption state between VHH-PM and VHH.

Keywords: solubility, ELISA, immobilization, VHH, PMMA-tag, isoelectric point, refolding

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1 Sewer Culvert Installation Method to Accommodate Underground Construction in an Urban Area with Narrow Streets

Authors: Osamu Igawa, Hiroshi Kouchiwa, Yuji Ito

Abstract:

In recent years, a reconstruction project for sewer pipelines has been progressing in Japan with the aim of renewing old sewer culverts. However, it is difficult to secure a sufficient base area for shafts in an urban area because many streets are narrow with a complex layout. As a result, construction in such urban areas is generally very demanding. In urban areas, there is a strong requirement for a safe, reliable and economical construction method that does not disturb the public’s daily life and urban activities. With this in mind, we developed a new construction method called the 'shield switching type micro-tunneling method' which integrates the micro-tunneling method and shield method. In this method, pipeline is constructed first for sections that are gently curved or straight using the economical micro-tunneling method, and then the method is switched to the shield method for sections with a sharp curve or a series of curves without establishing an intermediate shaft. This paper provides the information, features and construction examples of this newly developed method.

Keywords: micro-tunneling method, secondary lining applied RC segment, sharp curve, shield method, switching type

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